• Journal of the Korean Electrochemical Society
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• v.2 no.4
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• pp.227-231
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• 1999

Disposable, amperometric glucose sensor was constructed using platinised carbon paste electrode. The sensor response was studied by amperometry and cyclic voltammetry applying sample solutions on the strip-type electrode. Platinization of screen-printed carbon paste electrode effectively improved the electrochemical reversibility of a mediator and the analytical characteristics of the sensor. The heterogeneous rate constant for $[Fe(CN)_6]^{4-/3-}$ was $1.45\times10^{-2}cm{\cdot}s^{-1}$. An applied potential of 0.3V vs. Ag/AgCl resulted in the best selectivity for glucose. The apparent Michaelis-Menten constant for glucose on the strip sensor, $K_m^{app}$, was 24.5 mM. To evaluate the analytical performance of the glucose sensor strip, a correlation study was performed with the NOVA S.P, Ultra M analyzer for 30 serum samples containing $80\~297mg/dL$ of glucose: the correlation coefficient value was 0.983. It can be seen that the strip sensor has satisfactory precision and accuracy.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.2
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• pp.385-390
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• 2006

This paper examines the sampling and jitter specifications and considerations for Global Navigation Satellite Systems (GNSS) software receivers. Software radio (SWR) technologies are being used in the implementation of communication receivers in general and GNSS receivers in particular. With the advent of new GPS signals, and a range of new Galileo and GLONASS signals soon becoming available, GNSS is an application where SWR and software-defined radio (SDR) are likely to have an impact. The sampling process is critical for SWR receivers, where it occurs as close to the antenna as possible. One way to achieve this is by BandPass Sampling (BPS), which is an undersampling technique that exploits aliasing to perform downconversion. BPS enables removal of the IF stage in the radio receiver. The sampling frequency is a very important factor since it influences both receiver performance and implementation efficiency. However, the design of BPS can result in degradation of Signal-to-Noise Ratio (SNR) due to the out-of-band noise being aliased. Important to the specification of both the ADC and its clocking Phase- Locked Loop (PLL) is jitter. Contributing to the system jitter are the aperture jitter of the sample-and-hold switch at the input of ADC and the sampling-clock jitter. Aperture jitter effects have usually been modeled as additive noise, based on a sinusoidal input signal, and limits the achievable Signal-to-Noise Ratio (SNR). Jitter in the sampled signal has several sources: phase noise in the Voltage-Controlled Oscillator (VCO) within the sampling PLL, jitter introduced by variations in the period of the frequency divider used in the sampling PLL and cross-talk from the lock line running parallel to signal lines. Jitter in the sampling process directly acts to degrade the noise floor and selectivity of receiver. Choosing an appropriate VCO for a SWR system is not as simple as finding one with right oscillator frequency. Similarly, it is important to specify the right jitter performance for the ADC. In this paper, the allowable sampling frequencies are calculated and analyzed for the multiple frequency BPS software radio GNSS receivers. The SNR degradation due to jitter in a BPSK system is calculated and required jitter standard deviation allowable for each GNSS band of interest is evaluated. Furthermore, in this paper we have investigated the sources of jitter and a basic jitter budget is calculated that could assist in the design of multiple frequency SWR GNSS receivers. We examine different ADCs and PLLs available in the market and compare known performance with the calculated budget. The results obtained are therefore directly applicable to SWR GNSS receiver design.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.4
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• pp.438-445
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• 2017

Tanzania has abundant copper deposits, but copper-metal extraction remains low there, owing to the lack of suitable copper recovery processes and insufficient funds for developing mining technologies. Accordingly, leaching and solvent extraction methods for the extraction of copper from copper ore were studied with a particular emphasis on developing a simple processing method for small-scale copper mining. Chrysocolla ore was used as the copper-bearing mineral and sulfuric acid was used as the leaching reagent. A maximum copper recovery of 95.1% was obtained when the particles in the sample were smaller than $53{\mu}m$, the concentration of 98%(w/w) sulfuric acid in the leaching solution was 5.0 g/L and the stirring rate was between 60 and 80 rpm. The highest selectivity of $Cu^2+$ in the solvent extraction was obtained using 15% LIX-70 in kerosene. In the pH range from 0.5 to 3.0, the efficiency of $Cu^2+$ extraction increased with increasing pH. However, at pH values higher than 3.0, other metal ions were extracted into the organic phase more readily than $Cu^2+$. The highest solvent extraction rate obtained was 96.5% at pH values of 2.0 and 3.0 using 15% LIX-70.

• Applied Chemistry for Engineering
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• v.32 no.6
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• pp.684-689
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• 2021

In this paper, we develop a cost effective and disposable voltammetric sensing platform involving screen-printed carbon electrode (SPCE) modified with the nanocomposites composed of multi-walled carbon nanotubes, polyelectrolyte, and tyrosinase for bisphenol A. This is known as an endocrine disruptor which is also related to chronic diseases such as obesity, diabetes, cardiovascular and female reproductive diseases, precocious puberty, and infertility. A negatively charged oxidized multi-walled carbon nanotubes (MWCNTs) wrapped with a positively charged polyelectrolyte, e.g., polydiallyldimethylammonium, was first wrapped with a negatively charged tyrosinae layer via electrostatic interaction and assembled onto oxygen plasma treated SPCE. The nanocomposite modified SPCE was then immersed into different concentrations of bisphenol A for a given time where the tyrosinase reacted with OH group in the bisphenol A to produce the product, 4,4'-isopropylidenebis(1,2-benzoquinone). Cyclic and differential pulse voltammetries at the potential of -0.08 V vs. Ag/AgCl was employed and peak current changes responsible to the reduction of 4,4'-isopropylidenebis(1,2-benzoquinone) were measured which linearly increased with respect to the bisphenol A concentration. In addition, the SPCE based sensor showed excellent selectivity toward an interferent agent, bisphenol S, which has a very similar structure. Finally, the sensor was applied to the analysis of bisphenol A present in an environmental sample solution prepared in our laboratory.

• Analytical Science and Technology
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• v.36 no.3
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• pp.105-112
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• 2023

Lycopene, a carotenoid hydrocarbon is known to have effects on reducing cardiovascular risk factors, blood lipids, and blood pressure. Thus, a lot of supplementary foods with lycopene in several dosage forms like soft capsule filled with liquid and hard capsule filled with powder are available in a market. Recently, however, our research group found that the lycopene assay in Supplementary Food Code of South Korea is only valid for oily lycopene preparation. Thus, here, we developed a simple method to determine lycopene in solid preparations for Supplementary Food Code of South Korea using saponification and liquid chromatography with an absorbance detector. The method was validated following Ministry of Food and Drug Safety guidelines. All validation parameters observed in this study were within acceptable criteria of the guidelines (selectivity, linearity of r² ≥ 0.991, lower limit of quantification of 0.0149 mg/mL, accuracy as recovery (R) between 92.70 and 97.18 %, repeatability as relative standard deviation (RSD) values of R between 0.85 and 1.59 %, and reproducibility as the RSD value of interlaboratory R of 3.70 %). Additionally, the practical sample applicability of the validated method was confirmed by accuracy between 98.81 and 101.59 % observed from its lycopene certified reference material (CRM) analyses. Therefore, the present method could contribute to fortify the supplementary food safety management system in South Korea.

• Analytical Science and Technology
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• v.35 no.3
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• pp.136-142
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• 2022

According to media reports, the carcasses of euthanized abandoned dogs were processed at high temperature and pressure to make powder, and then used as feed materials (meat and bone meal), raising the possibility of residuals in the feed of the anesthetic ketamine and dexmedetomidine used for euthanasia. Therefore, a simultaneous analysis method using QuEChERS combined with high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry was developed for rapid residue analysis. The method developed in this study exhibited linearity of 0.999 and higher. Selectivity was evaluated by analyzing blank and spiked samples at the limit of quantification. The MRM chromatograms of blank samples were compared with those of spiked samples with the analyte, and there were no interferences at the respective retention times of ketamine and dexmedetomidine. The detection and quantitation limits of the instrument were 0.6 ㎍/L and 2 ㎍/L, respectively. The limit of quantitation for the method was 10 ㎍/kg. The results of the recovery test on meat and bone meal, meat meal, and pet food showed ketamine in the range of 80.48-98.63 % with less than 5.00 % RSD, and dexmedetomidine in the range of 72.75-93.00 % with less than 4.83 % RSD. As a result of collecting and analyzing six feeds, such as meat and bone meal, prepared at the time the raw material was distributed, 10.8 ㎍/kg of ketamine was detected in one sample of meat and bone meal, while dexmedetomidine was found to have a concentration below the limit of quantitation. It was confirmed that the detected sample was distributed before the safety issue was known, and thereafter, all the meat and bone meal made with the carcasses of euthanized abandoned dogs was recalled and completely discarded. To ensure the safety of the meat and bone meal, 32 samples of the meat and bone meal as well as compound feed were collected, and additional residue investigations were conducted for ketamine and dexmedetomidine. As a result of the analysis, no component was detected. However, through this investigation, it was confirmed that some animal drugs, such as anesthetics, can remain without decomposition even at high temperature and pressure; therefore, there is a need for further investigation of other potentially hazardous substances not controlled in the feed.

• Analytical Science and Technology
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• v.31 no.4
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• pp.161-170
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• 2018

The epidemic of disorders associated with synthetic stimulants, such as methamphetamine (MA) and amphetamine (AP), is a health, social, legal, and financial problem. Owing to the high potential of their abuse and addiction, reliable analytical methods are required to detect and identify MA, AP, and their metabolites in biological samples. Thus, a dilute-and-shoot liquid chromatography-tandem mass spectrophotometry (LC-MS/MS) was developed for simultaneous determination of MA, 4-hydroxymethamphetamine (4HMA), AP, and 4-hydroxyamphetamine (4HA) in urine. Urine sample ($100{\mu}L$) was mixed with $50{\mu}L$ of mobile phase consisting of 0.4 % formic acid and methanol and $50{\mu}L$ of working internal-standard solution. Aliquots of $8{\mu}L$ diluted urine was injected into the LC-MS/MS system. For all analytes, chromatographic separation was performed using a C18 reversed-phase column with gradient elution and a total run time of 5 min. The identification and quantification were performed by multiple reaction monitoring (MRM). Linear least-squares regression was conducted to generate a calibration curve, with $1/x^2$ as the weighting factor. The linear ranges were 2.0-200, 1.0-800, and 10-2500 ng/mL for 4HA and 4HMA, AP, and MA, respectively. The inter- and intraday precisions were within 6.6 %, whereas the inter- and intraday accuracies ranged from -14.9 to 11.3 %. The low limits of quantification were 2.0 ng/mL (4HA and 4HMA), 1.0 ng/mL (AP), and 10 ng/mL (MA). The proposed method exhibited satisfactory selectivity, dilution integrity, matrix effect, and stability, which are required for validation. Moreover, the purification efficiency of high-speed centrifugation was clearly higher than 6-15 % for QC samples (n=5), which was higher than that of the membrane-filtration method. The applicability of the proposed method was tested by forensic analysis of urine samples from drug abusers.

• Journal of the Korean Chemical Society
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• v.54 no.5
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• pp.556-566
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• 2010

A new kinetic spectrophotometric method is developed for the measurement of Mn(II) in natural water samples. The method is based on the catalytic effect of Mn(II) with the oxidation of Gallocyanin by $KIO_4$ using nitrilotriacetic acid (NTA) as an activation reagent at 620 nm. The optimum conditions obtained are $4.00{\times}1^{-5}\;M$ Gallocyanin, $KIO_4$, $1.00{\times}10^{-4}\;M$ NTA, 0.1 M HAc/NaAc buffer of pH = 3.50, the reaction time of 5 min and the temperature of $30^{\circ}C$. Under the optimum conditions, the proposed method allows the measurement of Mn(II) in a range of $0.1\;-\;4.0\;ng\;mL^{-1}$ and with a detection limit of down to $0.025\;ng\;mL^{-1}$. The recovery efficiency in measuring the standard Mn(II) solution is in a range of 98.5 - 102%, and the RSD is in a range of 0.76 - 1.25%. The newly developed kinetic method has been successfully applied to the measurement of Mn(II) in both some environmental water samples and certified standard reference river water sample, JAC-0031 with satisfying results. Moreover, few cations and anions interfere with the measurement of Mn(II). Compared with the other catalytic-kinetic methods and instrumental methods, the proposed kinetic method shows fairly good selectivity and sensitivity, low cost, cheapness, low detection limit and rapidity. It can easily and successfully be applied to the real water samples with relatively low salt content and complex matrices such as bottled drinking water, cold and hot spring waters, lake water, river water samples.

• Clean Technology
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• v.22 no.2
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• pp.122-131
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• 2016

Caesium radioisotopes, ¹³⁴Cs and ¹³⁷Cs which come from the atmospheric nuclear tests and discharges from nuclear power plants, are very important to study artificial radioactivity. In this work, in order to lower the minimum detection activity (MDA) we investigated environmental radioactivity according to the Environment Measurement Laboratory procedure by ¹³⁷Cs and ¹³⁴Cs which is similar to chemical and environmental behaviors of ¹³⁷Cs. The environmental soils in high mountain areas near nuclear power plant were collected, and an Ammonium Molybdophosphate (AMP) precipitation method, which showed high selectivity toward Cs⁺ ions, was applied to chemically extract and concentrate Caesium radioisotopes. Radioactivity was estimated by a gamma-ray spectrometry. In gamma energy spectrum, with an increasing of ⁴⁰K radioactivity, it increased the MDA of ¹³⁴Cs and ¹³⁷Cs. Therefore, if the natural radionuclides were removed from the soil samples, the MDA of Caesium may be reduced, and the contents of ¹³⁷Cs of in the environmental soils can effectively be estimated. In the standard soil sample of Korea Institute of Nuclear Safety, radioactivity of ⁴⁰K was removed more than 84% on average, and the MDA of ¹³⁴Cs was reduced 2 times. The content of ¹³⁷Cs was recovered over 84%. On the other hand, in environmental soils, AMP precipitation method showed removal ratio of ⁴⁰K up to 180 times, which reduced the MDA about 5 times smaller than those of Direct method. ¹³⁷Cs recovery ratio showed from 54.54% to 70.06%. When considering the MDA and recovery ratio, AMP precipitation method is effective for detection of Caesium radioisotopes in low concentration.