• Journal of Life Science
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• v.25 no.8
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• pp.910-916
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• 2015

Scientists have recently shown an interest in the characteristics of Bacillus mojavensis strains because of their increasing use in plants as a defense against diseases and mycotoxins. We have shown here that B. mojavensis KJS-3 possesses the typical characteristics of B. mojavensis strains including a strong resistance to high temperatures (≤50℃), tolerance to high salt concentrations (7% NaCl), ethanol tolerance (40% ethanol), and pH range for growth (pH 5-9). B. mojavensis KJS-3 has been used for the production of cyclic lipopeptides including important antifungal substances such as surfactin, iturin, and fengycin. Polymerase chain reaction analysis in this study showed that B. mojavensis KJS-3 can be used for the production of fengycin and the findings of LC-MS/MS analyses suggest that B. mojavensis KJS-3 can be used to produce iturin and surfactin. Antifungal activity analys is confirmed that B. mojavensis KJS-3 has antifungal effects on Botrytis cinerea, Rhizoctonia solani AG-4, Sclerotinia sclerotiorum, and Colletotricum goeosporioides. A microscopy assessment of the roots of wild ginseng plants planted together with B. mojavensis KJS-3 revealed that the roots contained B. mojavensis KJS-3, confirming the bacteria to be a plant growth promoting endophyte (PGPE) which acts against plant diseases and mycotoxins. Our findings lead us to conclude that B. mojavensis KJS-3 can be produced at an industrial level as a microbial pesticide or microbial fertilizer.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.5
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• pp.631-639
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• 2001

Two experiments were carried out concerning the effects of urea-molasses cake (UMC) and its separate components as supplements on rumen environment, in sacco feed degradability and intake of swamp buffaloes fed rice straw, grasses or a mixture of grasses and rice straw. Experiment 1 was a change-over design with 4 animals and 6 treatments. The buffaloes were fed rice straw ad libitum, and the experimental treatments were: no supplementation (R); 700 g of the complete urea-molasses cake (RUMC); 53.2 g urea (RU); 276 g rice bran and 52.5 coconut meal (RRC); 26.6 g salt, 26.6 g bone meal and 2.1 g trace minerals (RMi); and 25 g molasses (RMo). Experiment 2 was a Latin square design with four diets and four animals. The treatments were: rice straw ad libitum and mixed grass (RG) at 2.5 g dry matter per kg live weight (LW); RG plus 700 g urea-molasses cake (RGUMC); mixed grass ad libitum (G); and G plus 700 g cake (GUMC). In both experiments the supplements were fed once daily. In Exp. 1 although the rumen pH was significantly different (p<0.05) among diets, it varied only from 6.90 to 7.06. The ruminal ammonia was also significantly (p<0.05) different among the diets with RUMC significantly higher than R. Total bacterial and protozoal counts were significantly (p<0.05) higher for the RUMC, RU, RMo and RRC diets. Total feed and rice straw intakes were highest for RUMC (p<0.05) and lowest for the RMi and RMo diets, but in sacco degradability of four different roughages were not significantly different among diets. In Exp. 2, rumen pHs of the diets differed significantly and (p<0.01) ranged from 7.04 - 7.19. Ruminal $NH_3-N$ concentrations (mg/100 ml) were also significantly different (p<0.05), and higher for the RGUMC, G and GUMC diets. The total counts of bacteria and protozoa were significantly (p<0.05) higher for the RGUMC, G and GUMC diets. The total feed intake and roughage intake were significantly (p<0.05) higher for the RGUMC, G and GUMC diets compared to the RG diet. Correspondingly, LW changes also differed among treatments (p=0.06). It was concluded that there were significant increases in rumen $NH_3-N$ concentration, microbial populations and feed intake in the buffaloes by UMC supplementation, whereas the significant difference in in sacco DM degradation was not found by any type of supplementation. There seemed to be a need of a combination of urea, molasses, minerals and other protein nitrogen sources to enhance rice straw intake. Adding grass to the rice straw diet at 0.25% LW (DM) should also be considered to maintain buffalo rumen function and production with UMC supplementation, when rice straw is the main roughage.

• Culinary science and hospitality research
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• v.15 no.2
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• pp.150-160
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• 2009

This study analyzed the quality characteristics of demi-glace sauce with different addition of roux which is usually used as a thickener. The trust level and acceptability for commercial demi-glace sauce were surveyed in order to draw the visual idea and direction for the development of large scale commercial production of demi-glace sauce. Also, the optimal level of salt and roux content in the sauce was determined with various cooking conditions. As the roux content increased, the color became light and the viscosity of the sauce increased while pH, salinity and brix were not affected much by amount of roux. The professional chefs as well as general sensory panel preferred the demi-glace sauce prepared with 9% roux. To sum up, it can be claimed that the optimal conditions established in this study for commercial production of demi-glace sauce ensure the desired quality and economic feasibility.

• Microbiology and Biotechnology Letters
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• v.2 no.2
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• pp.79-82
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• 1974

Present experiments were designed to estimate the effects of pretreatments by various kinds of alkalis to the agricultural wastes such as cereal straws as the substrate on the production of cellulosic single-cell protein. Among the various kinds of alkalis NaOH was proved to be the most effective on improving the digestibility of cellulose by the bacteria isolated. NH$_4$OH which is inferior to NaOH in the effectiveness of treatment might have more economic advantage in the price, and the ammonium salt resulted from the neutralization can be used as the nitrogen source by bacteria. The treatment with higher concentration than 1 normality of NH$_4$OH didn't increase the productivility of cell mass. About five per cent of (NH$_4$)$_2$SO$_4$ in medium resulted from the neutralization didn't have any influence in the cell mass productivility. Futhermore, the cell mass productibility was higher in the case of neutralization than alkali free washing. The digestibility of straws was increased from 7.9％ to 46.0％ by NH$_4$OH treatment, and 6.3∼6.45g of dry cell were obtained from 40g of NH$_4$OH treated straws. In the case of NaOH treatment, 8.6g of cell mass was obtained from 40g of substrate.

• Applied Biological Chemistry
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• v.30 no.3
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• pp.250-257
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• 1987

The most active strain for the amylase activity from the native Youngkwang koji, was isolated and identified as Aspergjllus awamori. The optimal conditions for the production of ${\alpha}-amylase$ and glucoamylase were investigated and the properties of these enzymes were also examined. Maximum yields of ${\alpha}-amylase$ and glucoamylase were obtained at $30^{\circ}C$, pH 5.0 for days. The production of these two enzymes were increased with the addition of maltose, urea, $K_2HPO_4$, $MgSO_4{\cdot}7H_2O$, and $CaCl_2{\cdot}2H_2O$. The activities of these enzymes were maximized at $50^{\circ}C$, $pH\;5{\sim}6$. The heat stabilites of ${\alpha}-amylase$ and glucoamylase were maintained at $50^{\circ}C$ for 20min and 40min, respectively. Also, the addtion of $MgSO_4{\cdot}7H_2O$, $K_2HPO_4$, and $CaCl_2{\cdot}2H_2O$ salt increased the heat stabilities of these enzymes.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.2
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• pp.113-118
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• 2005

This experiment was conducted to compare the dry matter yields and fled values of winter forage crops using too cultivars of Barley, Rye md Italia ryegrass at the Dae-Ho reclaimed tideland, Korea from 2002 to 2004 2rowing season. Soil salt contents of three forage crops showed highest at sowing time, respectively and after wintering continuously lowed till harvest time. The dry matter yield was 6,668.8, 4,455.6 md 2,591.2 kg / ha, respectively, for Italian ryegrass, Barley and Rye. The highest Crude protein(CP) content was recorded in Italian ryegrass. Acid detergent fiber(ADF) contents were lowed in row with Barley, Italian ryegrass and Rye. Sodium contents in plant tissue were recorded high in line with Barley, Italian ryegrass and Rye. results mentions above suggest Italian ryegrass is suitable winter forage crops for cultivation on reclaimed tideland in view of the good emergence, forage production and its feed value.

• Journal of the Korea Organic Resources Recycling Association
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• v.9 no.4
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• pp.81-88
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• 2001

This experiment was carried out to evaluate the effect of residual food compost application on plant growth and physicochemical properties of soil when residual food compost made from the composting process as part of organic waste recycling was applied in soil as variable rate. The treatments were composed non-fertilizer treatment, control treatment applied with pig compost $20Mg(D.W.)ha^{-1}$, and residual food compost treatments each of applied with 20, 40, 60, $80Mg(D.W.)ha^{-1}$ and were randomized complete block design with three replication. Soil pH after experiment was more increased as more increase application of residual food compost. Other chemical properties of soil including EC were also showed the similar results. The plant growth in treatments applied much of residual food compost at the early growth stage was very damaged and these results were proved at the first growth measurement. Production of the red pepper especially reduced in treatments taken growth demage at the early stage and that of $20Mgha^{-1}$ treatment almost same as control treatment. Bulk density was reduced but porosity was increased according to increase of residual food compost application. Considering the reduction of red pepper production and the demage of growth at the early stage by plenty of salt and water soluble application volume of residual food compost was not permitted over of $30Mgha^{-1}$. Additively, to settle the application volume of residual food compost and to evaluate the plant growth and changes of physicochemical properties of soil, the results taken from continual applying is concluded more important than single application.

• Journal of Aquaculture
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• v.17 no.2
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• pp.151-157
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• 2004

Effects of supplementing selected fatty acids on fatty acid incorporation (17 days) , and progeny production (14 days) in Artemia franciscana (Great Salt Lake, USA) were studied. To compare with the control four diets, which differed in fatty acid composition alone contain Dunalieia tertiolecta and an emulsion either rich in OA (oleic acid, 18: 1 n-9), ARA (arachidonic acid, 20:4n-6), EPA (eicosapentaenoic acid,20:5n-3), or DHA (docosahexaenoic acid, 22:6n-3). Each of these emulsions was supplemented at a ratio of 20 % of the daily dose of D. tertiolecta (% algal dry weight). The initial OA and ARA values were 33.5 and 1.7 mg/g DW of freshly-hatched nauplii, respectively. After 11 days of feeding, these values increased to 38.8 and 7.6 mg/g DW in Artemia receiving the fatty acid sup-plement rich in each of the respective fatty acids. After 14 days, the levels were almost doubled, reaching 62.8 and 13.4 mg/g respectively. On EPA supplementation, its level after 11 days of feeding was 14.3 and 17.3 mg/g in male and female, respectively and was 16.0 and 23.1 mg/g in the male and female after 14 days, respectively. The EPA accumulated more in the body (39.1 mg/g) than in ovisac (16.9 mg/g). In the DHA supplementation group also, DHA levels after 11 days of feeding were 3.1 and 5.5 mg/g in male and female, respectively. After 14 days, the DHA level continued to increase in male. but slightly decreased to 4.6 mg/g in female. It was not richer in ovisac (2.6 mg/g) than in the remaining body of female (4.6 mg/g). In conclusion, fatty acids supplied by a lipid emulsion as a supplement to the algal diet are well incorporated in the adult Artemia. Apart from being an extra source of energy, these emulsions may function as source of HUFA which may play an essential role for growth and progeny production (fecundity) of Artemia.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.5
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• pp.445-454
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• 1997

Rice bran commonly available in Bangladesh is a mixture of rice hulls (60%), bran (35%) and polishing (5%), referred here as rice mill feed (RMF). Dose response effect of RMF supplementation to a straw diet including a zero level was measured on the intake, digestibility, nitrogen balance, microbial N yield and growth rate of growing native (Bos indicus) bulls. Twelve bulls of 33 months old and $272{\pm}31.5kg$ weight were randomly allocated to diets having 0 (T1), 1 (T2) and 2 (T3) kg RMF in addition to 200 g wheat bran, 200 g molasses, 60 g salt and 30 g oyestershe\l powder. Concentrate intake was 5.5, 19.2 and 29.5% of the dietary intake for the T1, T2 and T3 treatment respectively. RMF supplementation had no significant effect on the straw DM intake. However, with the increasing levels of RMF supplementation, total DM & digestible OM intake and the whole gut digestibilities of DM, OM, N & ADF increased but in deminishig return. Total microbial N yield estimated from the urinary purine excretion were 15.35, 26.56 and 38.44 g/d for the treatment T1, T2 and T3 respectively. Both the N intake and the N balance increased linearly in response to increasing level of RMF. Supplementation of RMF linearly increased the energy intake and dietary energy concentration. Growth rate in the T1, T2 and T3 treatments were 112, 125 and 250 g/d respctively. The basal N excretion and the maintenance energy requirement of the experimental animals were estimated to be 615 mg/kg $W^{0.75}/d$ and 447 kJ/kg $W^{0.75}/d$ respectively. The estimated efficiency on N utilization was 0.83 mg/mg of N intake ($r^2=0.997$) while the efficiency of metabolizable energy utilization for growth was 0.15. Since animal refused higher levels of RMF, inclusion up to 2 kg level (about 25% of the total DM intake) appears to have no depressing effect on the performances of animal. However, RMF itself fail to meet the critical nutrient need of the rumen microbes. Therefore response of supplementing RMF after correcting the critical nutrient deficiency need to be studied.