• 한국식품영양과학회지
• /
• 제39권12호
• /
• pp.1814-1818
• /
• 2010

본 연구에서는 타우린 첨가 김치의 발효과정 중 화학적 특성의 변화를 조사하였다. 대조군, Taurine I, Taurine II 및 Taurine III의 환원당과 비타민 C 함량은 발효 46일 후에 0일째와 비교하여 감소하였다. 대조군에서 타우린은 검출되지 않았으며, 타우린군의 경우 타우린 함량은 타우린첨가량에 비례하였고 발효기간 동안 타우린 함량은 크게 변하지 않았다. 이러한 결과로부터 첨가한 타우린이 김치 발효 숙성 중에 영향을 받지 않음을 알 수 있었다. 아미노태 질소 함량은 Taurine III> Taurine II> Taurine I> 대조군의 순이었다. 모든 분석 항목에서 타우린 첨가가 저온에서의 김치 발효에 영향을 미치지 않은 것으로 사료된다.

• 한국지역사회생활과학회지
• /
• 제14권3호
• /
• pp.89-98
• /
• 2003

To assess the actual status of businesses selling fermented soybean products, this study surveyed 130 Farmhouse style small size soybean sauce processing sites supported by the Rural Development Administration nationwide. The sex composition of the businesses representatives of 2% male and 98% female showed that a farmhouse soybean processing business is generally operated by rural women as a non-filming business. The percentage of co-worked sites was 71.2 and that of sites with permits was 39.2. The main products were meju, doenjang, and kanjang. Total production volume was found to be 523 tons, 256 tons, and 135 k$\ell$s, respectively. The number of years of experience of the manufacturers of the kanjang and doenjang was found to be 25.7 on average which shows that long-experienced, skilled manufacturers are participating in this project. The traditional measuring unit differed greatly depending on the region. This difference in the measuring unit of soybeans resulted in a large difference in the volume of doenjang and kanjang produced Per Province. For one kg of raw soybeans, the weight of Doenjang produced also varied from 2.14 kg in the Gyeongbuk area to 1.62 kg in Jeju. In the aspect of salt use, 1.75 $\ell$ (translated by volumetric unit 0.704 due) of salt pet kg of meju, in Jeju, was the highest in Korea. Government supervision would be beneficial in the areas of raw material products, standardization of measuring units and production methods, organization and operation of a conference for the different groups of producers, and in construction of a nationwide database.

• 한국동물위생학회지
• /
• 제35권1호
• /
• pp.47-52
• /
• 2012

Crocodile farms are getting popular in Bangladesh in an economic point of view. In one of the farms, some crocodiles were found sick and three of them died between May and July in 2006. This investigation was performed to diagnose the cause of the death. Routine postmortem examination was conducted. Samples were collected in 10% neutral buffered formalin for histopathology and in falcon tube for microbiological study. Additional swabs were collected in nutrient broth. Histopathological and microbiological studies were conducted using routine procedures. In addition Giemsa, Gram and PAS stains were performed to detect the organism in tissues. Grossly, esophagus, trachea, lungs, liver, spleen, heart and kidney were congested. Intestine, rectum and colon were hemorrhagic. Clay colored material was found in colo-rectum. Purulent exudates in lungs and thick and cloudy pericardial fluid in pericardial sac were found. Histologically, multifocal granulomatous inflammation was evident in lung, liver, kidney, intestine and colon with bacterial colonies, fungal spores and hyphae. These bacteria were appeared as Gram negative. Fungal hyphae and spores were detected in liver, lungs and colon by using PAS stain. Bacteriologically, E. coli were isolated from lungs exudates, pericardial fluids and intestinal fluids. Therefore, it can be concluded that 3 crocodiles died due to E. coli septicemia concurrent with mycotic infection.

• Journal of Microbiology and Biotechnology
• /
• 제18권5호
• /
• pp.821-827
• /
• 2008

The unicellular green alga Dunaliella salina is a halotolerant eukaryotic organism. Its halophytic properties provide an important advantage for open pond mass cultivation, since D. salina can be grown selectively. D. salina was originally described by E. C. Teodoresco in 1905. Since that time, numerous isolates of D. salina have been identified from hypersaline environments on different continents. The new Dunaliella strain used for this study was isolated from the salt farm area of the west coastal side of South Korea. Cells of the new strain were approximately oval- or pear-shaped (approximately $16-24\;{\mu}m$ long and $10-15\;{\mu}m$ wide), and contained one pyrenoid, cytoplasmatic granules, and no visible eyespot. Although levels of $\beta$-carotene per cell were relatively low in cells grown at salinities between 0.5 to 2.5 M NaCl, cells grown at 4.5 M NaCl contained about a ten-fold increase in cellular levels of $\beta$-carotene, which demonstrated that cells of the new Korean strain of Dunaliella can overaccumulate $\beta$-carotene in response to salt stress. Analysis of the ITS1 and ITS2 regions of the new Korean isolate showed that it is in the same clade as D. salina. Consequently, based on comparative cell morphology, biochemistry, and molecular phylogeny, the new Dunaliella isolate from South Korea was classified as D. salina KCTC10654BP.

• Asian-Australasian Journal of Animal Sciences
• /
• 제25권10호
• /
• pp.1466-1472
• /
• 2012

A bacterial isolate derived from soil samples near a cattle farm was found to display extracellular phytase activity. Based on 16S rRNA sequence analysis, the strain was named Bacillus sp. T4. The optimum temperature for the phytase activity toward magnesium phytate (Mg-$InsP_6$) was $40^{\circ}C$ without 5 mM $Ca^{2+}$ and $50^{\circ}C$ with 5 mM $Ca^{2+}$. T4 phytase had a characteristic bi-hump two pH optima of 6.0 to 6.5 and 7.4 for Mg-$InsP_6$. The enzyme showed higher specificity for Mg-$InsP_6$ than sodium phytate (Na-$InsP_6$). Its activity was fairly inhibited by EDTA, $Cu^{2+}$, $Mn^{2+}$, $Co^{2+}$, $Ba^{2+}$ and $Zn^{2+}$. T4 phytase may have great potential for use as an eco-friendly feed additive to enhance the nutritive quality of phytate and reduce phosphorus pollution.

• 대한수의학회지
• /
• 제47권3호
• /
• pp.299-302
• /
• 2007

Neoheterobothrium hirame (N. hirame) is a large blood-feed gill-worm infecting the highly prized olive flounder. The present study was reported the occurrence and elimination of Neoheterobothrium infection from young cultured oliver flounder in the western coast of Korea. Flounder (weight 18-25 g, length 10-15 cm) were obtained from a cultured farm at Taean of Korea at May 2006. A lot of flounder died with serious anemia. We isolated N. hirame from the buccal cavity wall of dead flounder. Adult parasites were 5-15 mm in total length, with 4 pairs of clamps on the pedunculated haptor and isthmus region embedded in host tissue, while immature and sub-adults were identified the attachment to the gill filaments, rakers and arches with the clamps. All fish with infection were treated with 8% NaCl-supplemented seawater for 1 and 10 min at $22^{\circ}C$, respectively. The significant reduction in mortality (6.7%) was observed among fish treated with 8% salt water for 10 minutes, when compared with that of non-treatment group (100%).

• Journal of Applied Biological Chemistry
• /
• 제48권1호
• /
• pp.11-15
• /
• 2005

Salinity of soils in greenhouse has been increased by massive application of fertilizers. Nitrogen fertilizer was most popular, and thus nitrate became the majority of soil salinity. Accumulation of nitrate led to deleterious effects on the growth and development of crops and vegetables. Microbial strains able to utilize nitrate and thus remove excess nitrate from farm land soils were isolated from 15 different soils of greenhouses and plastic film houses. Four strains able to grow in medium containing 50 mM $KNO_3$ were isolated, among which only E0461 showed high capacity of nitrate uptake. Nitrate uptake by E0461 was dependent on culture medium and was increased by addition of tryptone and peptone. Although E0461 was able to grow without tryptone and peptone, growth was slow, and no nitrate uptake was observed. Nitrate appeared to facilitate E0461 growth in the presence of tryptone and peptone. Through kinetic analysis, nitrate uptake was measured at various concentrations of nitrate, and half-life was calculated. Nitrate concentration decreased with increasing incubation period, and plot between half-lives and initial concentrations of nitrate fitted to single exponential function. These results suggest one major factor plays an important role in microbial nitrate uptake.

• 한국환경농학회지
• /
• 제40권2호
• /
• pp.83-91
• /
• 2021

본 연구는 폐자원 재활용 측면에서 농업적 유용활용(beneficial use)이 가능할 것으로 판단되는 영가철과 석탄회로 제조한 토양개량제를 투입하여 토양 염류 농도와 작물 생산량에 미치는 영향을 배양시험과 재배시험으로 평가하였다. 토양개량제는 석탄회를 구형으로 제형하고 영가철이 함유된 용액(0.1, 1.0%)을 표면에 살포하여 제조하였다. 토양개량제는 밭토양으로 충진된 와그너포트에 40 g/10a과 400 g/10a를 처리하였고, 30일 간 배양한 후 토양 EC와 염 농도를 분석하였다. 토양의 EC는 400 g/10a 실험구에서 대조구 대비 약 50% 감소한 것으로 나타났다. 국내 대표적인 염류인 유효인산의 경우 개량제의 처리 후 1/3 수준으로 감소되었다. 개량제 처리에 따른 토양 내 EC와 염 농도의 감소는 매우 유의한 수준으로 분석되었다(r=0.672~0.985). 또한 개량제 처리 후 뿌리와 줄기의 생육은 약 10% 증가였고, 건중량 또한 대조구에 비해 줄기와 뿌리에서 각각 30-50%와 60-75% 유의하게 증가하였다. 석탄회와 영가철을 농업용 토양개량제로 재활용할 경우 농업 환경 개선뿐만 아니라 농업 생산성 증대에도 기여할 수 있을 것으로 판단된다.

• 한국습지학회지
• /
• 제15권2호
• /
• pp.251-264
• /
• 2013

도요 물떼새들은 만조시에 해안 내륙 휴식지에서 휴식을 취하는데, 휴식지를 선택할 때 여러 가지 요소들을 고려한다. 우리는 도요 물떼새 8종(알락꼬리마도요 Numenius madagascariensis, 청다리도요 Tringa nebularia, 큰뒷부리도요 Limosa lapponica, 개꿩 Pluvialis squatarola, 민물도요 Calidris alpine, 중부리도요 Numenius phaeopus, 마도요 Numenius arquata, 뒷부리도요 Xenus cinereus)을 대상으로 휴식지의 시공간적 이용과 그 이용에 영향을 미치는 요인들을 분석하였다. 휴식지 내 고인 물의 면적과 길이, 기온, 풍속, 종별로 이동시기, 방해정도를 변수로 고려하였다. 시공간적으로, 이용하는 종이 달랐으며 개체수 또한 변동을 거듭하였다. 그리고 도요 물떼새의 휴식지 이용에 영향을 미치는 요인은 종-특이적이었다. 연구지역의 휴식지는 폐장한 새우양식장이었으나 조사 기간 중 염생습지에서 자라는 함초 Salicornia herbacea 농장으로 전환하였다. 휴식지가 필요한 상황에서, 도요 물떼새들의 생태를 기초로 해서 기존 휴식지를 관리할 것을 권한다. 더불어, 여름철 가뭄에는 양식장의 수문을 열어주어 도요 물떼새들이 물을 이용해 체온관리를 할 수 있게 해주고, 만조시 도요 물떼새들이 양식장 안으로 날아 들어올 수 있도록 새들이 휴식지를 찾기 시작하는 만조 전네 시간부터는 농장 작업을 안 할 것을 권한다.

• 한국농공학회논문집
• /
• 제60권3호
• /
• pp.113-121
• /
• 2018

The objectives of the research were 1) to develop the low-cost and high efficient desalination system to treat brackish water having high salt contents for irrigation at greenhouses near coast, and 2) to monitor and assess the effects of the brackish water desalination system on soil environment and growth in squash greenhouse cultivation area. The monitoring site was one of the squash greenhouse cultivation farm at Choengam-ri, Jinsang-myun, Gwangyang-si, Jeonnam-Do Monitoring results for groundwater irrigation water quality, and salinity showed a remarkable difference between control and treatment group. The salinity of soil at treatment group was less than at control group. While, the system made possible to increase the squash quantity from 4.7 ea to 6.3 ea at each and the average weight of the harvested squash was increased from 277.2 g to 295.1 g. The applied brackish water desalination system may be appled to reclaim sea or brackish irrigated area as alternative water resources, although long-term monitoring is needed to get more representative results at different level of salinity.