• Journal of Life Science
• /
• v.21 no.11
• /
• pp.1532-1540
• /
• 2011

The purpose of the present study was to investigate the effect of climbing resistance training on GLUT-4 protein and LDH isozyme activities of the soleus and gastrocnemius muscles in rats. Each experimental group was randomly divided into a control group (n=6) and a resistance exercise (n=6) group. Sprague-Dawley rats were made to climb a 180 cm tower for 12 wk. Weight changes in the resistance exercise group were significantly higher than in the control group (p<0.05). GLUT-4 protein expression of the soleus and gastrocnemius muscles was significantly higher (p<0.05) in the resistance exercise group than in the control group. There was no difference in soleus tissue LDHA4 isozyme activity between the groups. In the case of other LDH isozyme, when compared with the control group, the resistance exercise group showed a significantly higher activity (p<0.05). LDHA4 activity of gastrocnemius muscle tissue was not different between the groups. However, the activity of the resistance exercise group of all the other LDH isozymes was significantly higher than that of the control group (p<0.05). In summary, based on the results of this study, over 12 weeks of resistance training, the total body weight of the rats was reduced and the GLUT-4 activity in the gastrocnemius and soleus muscles was increased. In addition, except for LDH A4 all of the other LDH isozymes activities were increased. These results suggest that climbing resistance training affects the balance of body composition, increases LDH B-type isoenzymes and glucose metabolism capacity, and improves mitochondrial function.

• Journal of Korean Neurosurgical Society
• /
• v.62 no.6
• /
• pp.681-690
• /
• 2019

Objective : To determine whether the outcomes of percutaneous epidural neuroplasty (PEN) are influenced by the type of lumbar disc herniation (LDH) and evaluate the effectiveness of PEN in patients with single-level LDH. Methods : This study included 430 consecutive patients with single-level LDH who underwent PEN. Before treatment, the LDH type was categorized as bulging, protrusion, extrusion, and sequestration, while Pfirrmann grades were assigned according to imaging findings. Visual analog scale (VAS) scores for back and leg pain and success rates (Odom's criteria) were assessed at 1, 3, 6, and 12 months after treatment. Results : The mean preoperative VAS scores for back and leg pain were 6.90 and 4.23, respectively; these decreased after PEN as follows : 2.25 and 1.45, respectively, at 1 month; 2.61 and 1.68, respectively, at 3 months; 2.28 and 1.48, respectively, at 6 months; and 2.88 and 1.48, respectively, at 12 months (p<0.001). The decrease in VAS scores for leg pain was significantly greater in the extrusion and sequestration groups than in the other two groups (p<0.05); there were no other significant differences among groups. More than 70% patients exhibited good or excellent 12-month outcomes according to Odom's criteria. Subsequent surgery was required for 59 patients (13.7%), with a significantly higher rate in the extrusion (25.0%) and sequestration (30.0%) groups than in the bulging (7.3%) and protrusion (13.8%) groups (p<0.05). Nevertheless, subsequent surgery was not required for >70% patients with extrusion or sequestration. Patients with Pfirrmann grades 1-3 (14.0-21.5%) showed a significantly higher rate of subsequent surgery than those with Pfirrmann grade 0 (4.9%; p<0.05). Conclusion : Our findings suggest that PEN is an effective treatment for back and leg pain caused by single-level LDH, with the outcomes remaining unaffected by the LDH type.

• Journal of Korean Physical Therapy Science
• /
• v.11 no.4
• /
• pp.5-10
• /
• 2004

This study selected 10 university football male players and repeatedly treated them as sports massage group, exercise resumption group, and stability resumption. After maximum exercise, when sport massage, exercise resumption, and stability resumption were applied during recovery, blood lactic acid concentration and $LDH_5$ isozyme concentration were examined. Finally this study obtains the following conclusion 1. There was a significant difference in both lactic acid concentration and time between groups. Besides, the cross-action on group and time was significant. 2. There was a significant difference in both $LDH_5$ concentration and time between groups. Besides, the cross-action on group and time was significant. Based on the above findings, this study suggests that sport massage resumption and exercise resumption remove blood lactic acid more quickly than stability resumption to promote recovery. That means that sport massage and exercise resumption anre very effective for lowering the level of fatigue. Thus sport massage or exercise resumption treatment is considered to have a very positive effect on player's quick recovery and it should be used aggressively in a field.

• Journal of Oriental Neuropsychiatry
• /
• v.17 no.2
• /
• pp.37-59
• /
• 2006

Objective : This study was designed to asses the effect of Hwangryunhaedoktang and herbs on Mouse neuroblastoma 2a cells damaged by hypoxia-reoxygenation. Method : Mouse neuroblastoma 2a (N2a) cells were measured by MTT assay and LDH assay after 48h hypoxia and 6h reoxygenation. Mouse neuroblastoma 2a (N2a) cells were treated by Hwangryunhaedoktang and herbs. Result : 1. Hwangryunhaedoktang was effective on LDH assay of hypoxia and reoxygenation. 2. All of herbs were generally effective on LDH assay of hypoxia and reoxygenation. In LDH assay of hypoxia, the effects of herbs depended on concentration. In MTT assay of hypoxia, Coptidis Rhizoma and Gardeniae Fructus were effective. In MTT assay of reoxygenation most of herbs were not effective. But Phellodendri Cortex was effective in high concentration. Conclusion : The results imply that Hwangryunhaedoktang and all herbs of it nay have protective effect on dementia and aging.

• Journal of Oriental Neuropsychiatry
• /
• v.16 no.1
• /
• pp.19-41
• /
• 2005

Object : This study was designed to asses the effect of Sunghyangjungkisan-ga-pogongng and herbs on Mouse neuroblastoma 2a cells damaged by hypoxia-reoxygenation. Method : Mouse neuroblastoma 2a (N2a) cells were measured by MTT assay and LDH assay after 48h hypoxia and 6h reoxygenation, Mouse neuroblastoma 2a (N2a) cells were treated by SHJG+P and herbs. Result : 1. SHJG+P was effective on LDH assay of hypoxia and reoxygenation. 2. The herbs were generally effective on LDH assay of hypoxia and reoxygenation. In MTT assay of hypoxia JP and GC were effecctive. In LDH assay of hypoxia all of herbs were effective. DMH, BC, SY, NS were more effective than other herbs. In LDH assay of reoxygenation KH, BH, BBR, DMH were especially effective. In MTT assay of reoxygenation most of herbs were not effective. But GC, SY, BH, JP were effective. Conclusion : The results imply that SHJG+P and all of berbs may have protective effect on dementia and GC, SY, BH, JP may have protective effect.

• Journal of International Academy of Physical Therapy Research
• /
• v.10 no.1
• /
• pp.1725-1733
• /
• 2019

Background: In some clinical guidelines followed in clinical practice, nonsurgical treatments are recommended as the primary intervention for patients with lumbar disc herniation (LDH). However, the effect of a therapeutic exercise program based on stabilization of the lumbar spine for treatment of multilevel LDH has not been evaluated thoroughly. Objective: To investigate the effects of therapeutic exercise on pain, physical function, and magnetic resonance imaging (MRI) findings in a patient with multilevel LDH. Design: Case Report Methods: A 43-year-old female presented with low back pain, radicular pain and multilevel LDH (L3-L4, L4-L5, L5-S1). The therapeutic exercise program was conducted. in 40-min sessions, three times a week, for 12 weeks. Low back and radicular pain, lumbar disability, and physical function were measured before and after 6 and 12 weeks of the exercise program. MRI was performed before and after 12 weeks of the program. Results: After 6 and 12 weeks of the therapeutic exercise, low back and radicular pain and lumbar disability had decreased, and lumbar range of motion (ROM) was improved bilaterally, compared with the initial values. Also improved at 6 and 12 weeks were isometric lumbar strength and endurance, and the functional movement screen score. The size of disc herniations was decreased on MRI obtained after 12 weeks of therapeutic exercise than on the pre-exercise images. Conclusions: We observed that therapeutic exercise program improved spinal ROM, muscle strength, functional capacity, and size of disc herniation in LDH patient.

• The Korean Journal of Pain
• /
• v.30 no.1
• /
• pp.44-50
• /
• 2017

Background: Low back pain is a frequent condition that results in substantial disability and causes admission of patients to neurosurgery clinics. To evaluate and present the therapeutic outcomes in lumbar disc hernia (LDH) patients treated by means of a conservative approach, consisting of bed rest and medical therapy. Methods: This retrospective cohort was carried out in the neurosurgery departments of hospitals in KahramanmaraŞ city and 23 patients diagnosed with LDH at the levels of L3-L4, L4-L5 or L5-S1 were enrolled. Results: The average age was $38.4{\pm}8.0$ and the chief complaint was low back pain and sciatica radiating to one or both lower extremities. Conservative treatment was administered. Neurological examination findings, durations of treatment and intervals until symptomatic recovery were recorded. $Las{\grave{e}}gue$ tests and neurosensory examination revealed that mild neurological deficits existed in 16 of our patients. Previously, 5 patients had received physiotherapy and 7 patients had been on medical treatment. The number of patients with LDH at the level of L3-L4, L4-L5, and L5-S1 were 1, 13, and 9, respectively. All patients reported that they had benefit from medical treatment and bed rest, and radiologic improvement was observed simultaneously on MRI scans. The average duration until symptomatic recovery and/or regression of LDH symptoms was $13.6{\pm}5.4$ months (range: 5-22). Conclusions: It should be kept in mind that lumbar disc hernias could regress with medical treatment and rest without surgery, and there should be an awareness that these patients could recover radiologically. This condition must be taken into account during decision making for surgical intervention in LDH patients devoid of indications for emergent surgery.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.12
• /
• pp.1670-1674
• /
• 2001

To understand molecular mechanisms that regulate deposition and release of intramuscular fat, a fasting-induced clone was identified by differential screening from cDNA library of adipose tissues of Korean cattle. The clone had a total length of 1,319 nucleotides coding for 334 amino acids. It was identified as one encoding L-lactate dehydrogenase H chain (LDH-B). Comparison of the deduced amino acid sequences of bovine LDH-B with those of pig, human, rat, and mouse showed 98%, 98%, 97%, and 96% identity, respectively. Food deprivation for 48 h increased mRNA levels of LDH-B gene in adipose tissues of Korean cattle compared to fed- and 6 h refed- tissues. The expression of obese mRNA was examined for individual adipose tissue from several fat depots. Fasting induced expression of LDH-B gene in subcutaneous adipose tissues, but it did not affect expression levels in abdominal, perirenal and intramuscular tissues. Results demonstrate that induction of LDH-B gene during fasting may represent a metabolic shift from anaerobic state to aerobic predominance in fasted adipose tissues and that its responses to fasting are different among several adipose tissues.

• Applied Biological Chemistry
• /
• v.13 no.1
• /
• pp.93-96
• /
• 1970

In order to study the difference in metabolic efficiency of the cotyledon and seedlings of soybean during germination, the authors assayed lactic dehydrogenase activities in the two tissues for the purpose of comparison, with the following conclusions. 1. The LDH activity in the cotyledon of soybean increases during the 3 days after germination, followed by abrupt decrease during the later 3 days of germination. 2. The LDH activity in the seedlings of soybean increases during the 3 days after germination under fairly good correlation with the variation observed in the cotyledon; but, unlike the cotyledon LDH, the activity remains high through the later 3days of germination. 3. It is, therefore, concluded that the metabolic efficiency in the cotyledon seems to begin to stop at the 3rd day following germination, while the efficiency in the seedlings remains high.

• Korean Journal of Pharmacognosy
• /
• v.22 no.1
• /
• pp.36-44
• /
• 1991

This study was attempted to investigate the acute toxicity of Liocolae vermiculus(Liocola brevitarsis) extract in mice, the effect on GOT, GPT Al.p, LDH activities and level of total cholesterol in serum of $CCl_4-intoxicated$ rats. In acute toxicity test, Liocolae vermiculus extract showed 10% mortality at 2,000 mg/kg, p.o. and at 1,000 mg/kg, i.p.. The Liocolae verculus extract caused a remarkable decrease in serum transaminase as well as Al.p activities in $CCl_4-intoxicated$ rats at $300{\sim}1,000{\;}mg/kg$ dosage ranges. The activities of -LDH and the level of total cholesterol were significantly decreased in all sample-treated group, when compared with the control group. The body weight decreased, and the liver and spleen weight increased in $CCl_4-intoxicated$ rats were significantly recovered by the administration of the extracts.