Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Cloning and Expression of Lactate Dehydrogenase H Chain Gene in Adipose Tissues of Korean Cattle

  • Kim, H.H. (Department of Genetic Engineering, Biotechnology Research Institute Chonnam National University) ;
  • Seol, M.B. (Department of Genetic Engineering, Biotechnology Research Institute Chonnam National University) ;
  • Jeon, D.H. (Department of Genetic Engineering, Biotechnology Research Institute Chonnam National University) ;
  • Sun, S.S. (Department of Animal Science, Biotechnology Research Institute, Chonnam National University) ;
  • Kim, K.H. (Department of Animal Science, Biotechnology Research Institute, Chonnam National University) ;
  • Choi, Y.J. (School of Agricultural Biotechnology, Seoul National University) ;
  • Baik, M.G. (Department of Genetic Engineering, Biotechnology Research Institute Chonnam National University)
  • Received : 2001.03.02
  • Accepted : 2001.07.11
  • Published : 2001.12.01
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Abstract

To understand molecular mechanisms that regulate deposition and release of intramuscular fat, a fasting-induced clone was identified by differential screening from cDNA library of adipose tissues of Korean cattle. The clone had a total length of 1,319 nucleotides coding for 334 amino acids. It was identified as one encoding L-lactate dehydrogenase H chain (LDH-B). Comparison of the deduced amino acid sequences of bovine LDH-B with those of pig, human, rat, and mouse showed 98%, 98%, 97%, and 96% identity, respectively. Food deprivation for 48 h increased mRNA levels of LDH-B gene in adipose tissues of Korean cattle compared to fed- and 6 h refed- tissues. The expression of obese mRNA was examined for individual adipose tissue from several fat depots. Fasting induced expression of LDH-B gene in subcutaneous adipose tissues, but it did not affect expression levels in abdominal, perirenal and intramuscular tissues. Results demonstrate that induction of LDH-B gene during fasting may represent a metabolic shift from anaerobic state to aerobic predominance in fasted adipose tissues and that its responses to fasting are different among several adipose tissues.

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