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Comparison between the liquid-liquid partition method and modified QuEChERS method for the analysis of pesticide residues in beef fat (소지방 중 잔류농약분석을 위한 액-액분배법과 modified QuEChERS법 비교)

  • Kim, Yoen-Joo;Choi, Yoon-Hwa;Shin, Bang-Woo;Lee, Jung-Hark
    • Korean Journal of Veterinary Service
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    • v.34 no.4
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    • pp.429-439
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    • 2011
  • This article described the comparison of a quick, easy, cheap, effective, rugged and safe (QuEChERS) sample preparation and the classical method established by National Veterinary Research and Quarantine Service (NVRQS) for the determination of pesticide residues in livestock products using GC-tandem mass spectrometry. The classical method by NVRQS used liquid-liquid partioning followed by evaporizing. The modified QuEChERS entailed extraction of 2 g sample with 15 ml acetonitrile containing 1% acetic acid followed by addition of 6 g anhydrous magnesium sulfate and 1.5 g sodium acetate. After centrifugation, 6 ml of the extract underwent a cleanup step (in a technique known as column-based solid phase extraction) using 400 mg each of $C_{18}$ and primary secondary amine sorbents plus 1,200 mg magnesium sulfate. The quantitation of individual pesticides by both methods was based on tissue standard calibration curves with a correlation coefficient in excess of 0.98 for the 24 pesticides. The detection limits by the classical method were ranged 1.3~5.0 ${\mu}g$/kg, with mean recoveries between 76.2% and 114.3% except aldrin (59.3%) and deltamethrin (63.6%). The detection limits by modified QuEChERS were ranged 0.3~6.2 ${\mu}g$/kg, with mean recoveries between 68.0% and 114.3% except dimethipin (152.6%), chlorfenvinphos (138.1%), 4,4-DDT (61.5%), aldrin (60.4%) and chinomethionate (30.3%).

Infection Properties of Oak Wilt Disease in Bukhansan National Park Adjacent to Metropolitan Areas in Korea

  • Choi, Jin-Woo;Yeum, Jung-Hun
    • Journal of Environmental Science International
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    • v.26 no.7
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    • pp.803-815
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    • 2017
  • In this study of the oak wilt disease that has occurred in a large scale in a protected area located near South Korea's metropolitan region, a detailed analysis has been conducted on the terrain, species and Diameters at Breast Height (DBH) of infected trees to identify the distribution of infection properties in the affected area. Taking into consideration the distribution of oak tree vegetation, a total of 4,640 quadrats in a size of 10 m by 10 m, have been set; and oak tree species, the DBH and infection damage per quadrat have been investigated. Geological properties have been analyzed according to elevation, slope, aspect and micro topography while a weighted value has been given according to the degree of infection in order to calculate an infection index. Through correlation analysis, the infection ratio of seriously-damaged and withered trees and the infection index have been analyzed with regards to the geological properties, tree species and DBH. The analysis shows that the disease tends to affect an area with medium elevation rather than those in the highest or lowest areas and that serious damage has been observed at rugged spots with a steep gradient (more than $30^{\circ}$). Although there has been no distinct tendency with regards to aspect, the infection ratio is relatively high in areas facing the north while the seriously-damaged and withered ratio are high in areas facing the south. In terms of micro topography, more damage has been spotted in valley terrain. Quercus mongolica has sustained more damage than other species. When it comes to the DBH, as seen in previous studies, large trees have suffered severe damage, but the analysis has also revealed conspicuous damage to medium trees with a DBH of 15-20 cm, which had not previously been considered at high risk.

Korean Groal Potential Habitat Suitability Model at Soraksan National Park Using Fuzzy Set and Multi-Criteria Evaluation (설악산국립공원내 산양(Nemorhaedus Caudatus Raddeanus)의 잠재 서식지 적합성 모형; 다기준평가기법(MCE)과 퍼지집합(Fuzzy Set)의 도입을 통하여)

  • Choi Tae-Young;Park Chong-Hwa
    • Journal of the Korean Institute of Landscape Architecture
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    • v.32 no.4
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    • pp.28-38
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    • 2004
  • Korean goral (Nemorhaedus caudatus raddeanus) is one of the endangered species in Korea, and the rugged terrain of the Soraksan National Park (373㎢) is a critical habitat for the species. But the goral population is threatened by habitat fragmentation caused by roads and hiking trails. The objective of this study was to develop a potential habitat suitability model for Korean goral in the park, and the model was based on the concepts of fuzzy set theory and multi-criteria evaluation. The process of the suitability modeling could be divided into three steps. First, data for the modeling was collected by using field work and a literature survey. Collected data included 204 points of GPS data obtained through a goral trace survey and through the number of daily visitors to each hiking trail during the peak season of the park. Second, fuzzy set theory was employed for building a GIS data base related to environmental factors affecting the suitability of the goral habitat. Finally, a multiple-criteria evaluation was performed as the final step towards a goral habitat suitability model. The results of the study were as follows. First, characteristics of suitable habitats were the proximity to rock cliffs, scattered pine (Pinus densiflora) patches, ridges, the elevation of 700∼800m, and the aspect of south and southeast. Second, the habitat suitability model had a high classification accuracy of 93.9% for the analysis site, and 95.7% for the validation site at a cut off value of 0.5. Finally, 11.7% of habitatwith more than 0.5 of habitat suitability index was affected by roads and hiking trails in the park.

The Implementation of High speed Memory module Interface in the Military Single Board Computer (군용Single Board Computer에서의 고속메모리모듈 I/F구현)

  • Lee, Teuc-Soo;Kim, Young-Kil
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.15 no.3
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    • pp.521-527
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    • 2011
  • POWER PC series are common to the Central Processing Unit for Military Single Board Computer. Among them, G4 group, which contains the 74xx series supported by Freescale manufacturer is mainly used in the Military applications. We focus on the Interface between memory and controller. PCB stacking method, component routing, impedance matching and harsh environment for Military spec are the main constraints for implementation. Also, we developed memory as a module for the consideration of Military environments. The overall type of SBC should be designed by the form of 6U VME or 3U VME. Therefore this study suggests the electrically optimum Interface matching, Artwork technology based on the signal cross over and PCB stacking method on the harsh environment.

The Fabrication and Electrical Characteristics of Pentacene TFT using Polyimide and Polyacryl as a Gate Dielectric Layer (Polymide와 Polyacryl을 게이트 절연층으로 이용한 pentacene TFT의 제작과 전기적 특성에 관한 연구)

  • Kim, Yun-Myoung;Kim, Ok-Byoung;Kim, Young-Kwan;Kim, Jung-Soo
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.50 no.4
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    • pp.161-168
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    • 2001
  • Organic thin film transitors(TFTs) are of interest for use in broad area electronic applications. For example, in active matrix liquid crystal displays(AMLCDs), organic TFTs would allow the use of inexpensive, light-weight, flexible, and mechanically rugged plastic substrates as an alternative to the glass substrates needed for commonly used hydrogenated amorphous silicon(a-Si:H). Recently pentacene TFTs with carrier field effect, mobility as large as 2 $cm^2V^{-1}s^{-1}$ have been reported for TFTs fabricated on silicon substrates, and it is higher than that of a-Si:H. But these TFTs are fabricated on silicon wafer and $SiO_2$ was used as a gate insulator. $SiO_2$ deposition process requires a high insulator which is polyimide and photo acryl. We investigated trasfer and output characteristics of the thin film transistors having active layer of pentacene. We calculated field effect mobility and on/off ratio from transfer characteristics of pentacene thin film transistor, and measured IR absorption spectrum of polymide used as the gate dielectric layer. It was found that using the photo acryl as a gate insulator, threshold voltage decreased from -12.5 V to -7 V, field effect mobility increased from 0.012 $cm^2V^{-1}s^{-1}$ to 0.039 $cm^2V^{-1}s^{-1}$ , and on/off current ratio increased from $10^5\;to\;10^6$. It seems that TFTs using photo acryl gate insulator is apt to form channel than TFTs using polyimide gate insulator.

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Ecological Features of 'Pingguoli'(Pyrus sp.) Fruits Produced in Korea and China (한국산과 중국산 '사과배' 과실의 생태적 특성)

  • Piao, Yi-Long;Hwang, Yong-Soo;Lee, Jae-Chang
    • Korean Journal of Agricultural Science
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    • v.29 no.1
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    • pp.1-7
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    • 2002
  • Postharvest characteristics of 'Pingguoli' fruit produced between China and Korea were compared to find an ecological difference. There was a difference in fresh weight per fruit, thus, fruit weight produced in Korea was much higher than those in China. However, no significant differences were confirmed in the level of soluble solid content and flesh firmness. The acidity of Korean 'Pingguoli' was higher than Chinese ones. The external appearance of Korean 'Pingguoli' was not attractive due to the rough and rugged surface. Thus, the prospect of 'Pingguoli' cultivation in Korea seems not to be promised. The general trend of respiration and ethylene evolution during storage did not significantly differed but the amount of $CO_2$ and ethylene production was higher in fruits produced in China. There was a strong similarity in respiration and ethylene evolution pattern to typical climacteric fruits. Thus, 'Pingguoli' is considered as a climacteric fruit. Even though the rate of ethylene production of 'Pingguoli' was relatively high, the storability of 'Pingguoli' showed a strong potential. The further studies in postharvest physiology of pear using 'Pingguoli' and in its breeding potentials as a source of long storage life are needed.

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Simultaneous Analysis of Conazole Fungicides in Garlic by Q-TOF Mass Spectrometer Coupled with a Modified QuEChERS Method

  • Bong, Min-Sun;Yang, Si-Young;Lee, Seung-Ho;Seo, Jung-Mi;Kim, In-Seon
    • Korean Journal of Environmental Agriculture
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    • v.30 no.3
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    • pp.323-329
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    • 2011
  • BACKGROUND: The conazoles, difenoconazole, diniconazole, hexaconazole, penconazole and tetraconazole are a large class of synthetic fungicides used extensively for foliage and seed treatments in agricultural crops. The extensive use of conazoles has brought concerns on the potentiality of environmental contamination and toxicity. Thus studies on the development of methods for monitoring the conazoles are required. METHODS AND RESULTS: A modified quick, easy, effective, rugged and safe (QuEChERS) method was involved in sample preparation. Quadrapole time of flight mass spectrometer (Q-TOF MS) in electron spray ionization (ESI) mode was employed to determine conazoles in garlic samples. The limit of detection (LOD) and limit of quantification (LOQ) of conazoles by Q-TOF-MS ranged from 0.001 to 0.002 mg/L and 0.002 to 0.005 mg/L, respectively. Q-TOF-MS analysis exhibited less than 2.6 ppm error of accurate mass measurements for the detection of conazoles spiked at 0.05 mg/L in garlic matrix. Recovery values of conazoles fortified in garlic samples at 0.02, 0.05 and 0.1 mg/L were between 79.2 and 106.2% with a maximum 11.8% of standard deviation. No detectable conazoles were found in the domestic market samples by using the Q-TOF-MS method. CONCLUSION(s): High degree of confirmation for conazoles by accurate mass measurements demonstrated that Q-TOF-MS analysis combined with a QuEChERS method may be applicable to simultaneous determination of conazoles in garlic samples.

Development of Simultaneous Analytical Method for Thiodicarb and its Metabolite Methomyl in Livestock Products (축산물 중 Thiodicarb와 대사산물 Methomyl의 동시분석법개발)

  • Chang, Hee-Ra;You, Jung-Sun;Ban, Sun-Woo;Gwak, Hye-min
    • Korean Journal of Environmental Agriculture
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    • v.40 no.2
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    • pp.142-147
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    • 2021
  • BACKGROUND: Agricultural use and pest control purposes of pesticides may lead to livestock products contamination. Thiodicarb and its degraded product, methomyl, are carbamate insecticides that protect soya bean, maize, fruit, and vegetables and control flies in animal and poultry farms. For maximum residue limit enforcement and monitoring, the JMPR residue definition of thiodicarb in animal products is the sum of thiodicarb and methomyl, expressed as methomyl. This residue definition was set to consider the fact that thiodicarb was readily degraded to methomyl in animal commodities. And therefore the simultaneous analytical method of thiodicarb and methomyl is required for monitoring in livestock products. METHODS AND RESULTS: The study was conducted using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and HPLC-MS/MS to determine the thiodicarb and methomyl in livestock products. The limit of quantitation (LOQ) was 0.01 mg/kg for livestock products, including beef, pork, chicken, milk, and egg. The coefficient of determinations (r2) for the calibration curve were > 0.99, which was acceptable values for linearity. Average recoveries at spiked levels (LOQ, 10LOQ, and 50LOQ, n=5) in triplicate ranged from 73.2% to 102.1% and relative standard deviations (RSDs) were less than 10% in all matrices. CONCLUSION: The analytical method was validated for the performance parameters (specificity, linearity, accuracy, and precision) in livestock products to be acceptable by the CODEX guidelines.

Evaluation and application of pretreatment methods for pharmaceuticals and personal care products in the solid phase of sewage samples (하수처리시설 고상시료 중 잔류의약물질 분석을 위한 전처리법 평가 및 적용)

  • Park, Junwon;Kim, Changsoo;Ju, Byoungkyu;Lee, Wonseok;Chung, Hyenmi;Jeong, Dong-Hwan
    • Journal of Korean Society of Water and Wastewater
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    • v.32 no.6
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    • pp.559-572
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    • 2018
  • The aim of this study was to evaluate pretreatment methods for 27 pharmaceuticals and personal care products (PPCPs) in various sewage samples using a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) and online solid-phase extraction with LC-MS/MS. Extraction efficiencies of PPCPs in the solid phase under different experimental conditions were evaluated, showing that the highest recoveries were obtained with the addition of sodium sulfate and ethylenediaminetetraacetic acid disodium salt dehydrate in acidified conditions. The recoveries of target compounds ranged from 91 to 117.2% for liquid samples and from 61.3 to 137.2% for solid samples, with a good precision. The methods under development were applied to sewage samples collected in two sewage treatment plants (STPs) to determine PPCPs in liquid and solid phases. Out of 27 PPCPs, more than 19 compounds were detected in liquid samples (i.e., influent and effluent) of two STPs, with concentration ranges of LOQ-33,152 ng/L in influents and LOQ-4,523 ng/L in effluents, respectively. In addition, some PPCPs such as acetylsalicylic acid, ibuprofen, and ofloxacin were detected at high concentrations in activated sludge as well as in excess sludge. This methodology was successfully applied to sewage samples for the determination of the target compounds in STPs.

GLSL based Additional Learning Nearest Neighbor Algorithm suitable for Locating Unpaved Road (추가 학습이 빈번히 필요한 비포장도로에서 주행로 탐색에 적합한 GLSL 기반 ALNN Algorithm)

  • Ku, Bon Woo;Kim, Jun kyum;Rhee, Eun Joo
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.12 no.1
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    • pp.29-36
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    • 2019
  • Unmanned Autonomous Vehicle's driving road in the national defense includes not only paved roads, but also unpaved roads which have rough and unexpected changes. This Unmanned Autonomous Vehicles monitor and recon rugged or remote areas, and defend own position, they frequently encounter environments roads of various and unpredictable. Thus, they need additional learning to drive in this environment, we propose a Additional Learning Nearest Neighbor (ALNN) which is modified from Approximate Nearest Neighbor to allow for quick learning while avoiding the 'Forgetting' problem. In addition, since the Execution speed of the ALNN algorithm decreases as the learning data accumulates, we also propose a solution to this problem using GPU parallel processing based on OpenGL Shader Language. The ALNN based on GPU algorithm can be used in the field of national defense and other similar fields, which require frequent and quick application of additional learning in real-time without affecting the existing learning data.