• Title/Summary/Keyword: rotifera

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Effects of Photoperiod Exchanges on the Growth of two Common Live Food Organisms (rotifer, Brachionus rotundiformis and Harpacticoida Copepoda, Tigriopus japonicus) in the Combination Cultures (Brachionus rotundiformis (rotifera)와 Tigriopus japonicus(copepoda; harpacticoida)의 혼합배양에 있어서 광주기 변화가 두 동물먹이생물의 증식에 미치는 영향)

  • Jung, Min-Min;Moon, Tae-Seok;Wi, Chong-Hwan;Ji, Young-Ju;Min, Kwang-Sik
    • Journal of Aquaculture
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    • v.20 no.3
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    • pp.168-172
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    • 2007
  • Two zooplankton species Brachionus rotundiformis (rotifera) and Tigriopus japonicus (copepoda; harpacticoida) were commonly used as live food organisms in the marine larval rearing centers. The combination culture method of two live food organisms (B. rotundiformis and T. japonicus) was well known as very valuable for stable and mass cultures. In this study, we investigated the effects of photoperiod exchanges on the growth and interspecific relationship in the combination culture of two species. The results showed that, photoperiod condition can change copepod (and rotifer) density under the two species combination cultures. There is 200% higher maximum rotifer density on the 24L:0D photoperiod culture condition compare to 12L:12D condition. However, maximum density of copepod is observed highest on the 0L:24D photoperiod culture condition. In addition, it's differ in the culture densities of nauplii, copepodites and female carrying eggs on the each three photoperiod types.

Molecular Monitoring of Plankton Diversity in the Seonakdong River and Along the Coast of Namhae (분자 모니터링을 이용한 서낙동강과 남해 연안 플랑크톤 군집 분석)

  • Kim, Bo-Kyung;Lee, Sang-Rae;Lee, Jin-Ae;Chung, Ik-Kyo
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.15 no.1
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    • pp.25-35
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    • 2010
  • The biodiversity of eukaryotic plankton has commonly been used to evaluate the status of aquatic ecosystems. Therefore, an accurate and rapid method for species identification is needed to reveal the biodiversity of environmental water samples. To date, molecular methods have provided a great deal of information that has enabled identification of the hidden biodiversity in environmental samples. In this study, we utilized environmental polymerase chain reaction (PCR) and constructed the 18S nuclear ribosomal RNA clone library from environmental water samples in order to develop more efficient methods for species identification. For the molecular analysis, water samples were collected from the Seonakdong River (Gimhae Bridge) and the coast of Namhae,(Namhaedo). Colony PCR and restriction fragment length polymorphism of PCR (PCR-RFLP) were then adopted to isolate unique clones from the 18S rDNA clone library. Restriction fragment length polymorphism pattern analysis of the Gimhae Bridge sample revealed 44 unique clones from a total of 60 randomly selected clones, while analysis of the Namhae sample revealed 27 unique clones from 150 clones selected at random. A BLAST search and subsequent phylogenetic analysis conducted using the sequences of these clones revealed hidden biodiversity containing a wide range of taxonomic groups (Heterokontophyta (7), Ciliophora (23), Dinophyta (1), Chytridiomycota (1), Rotifera (1) and Arthropoda (11) in the Gimhae Bridge samples Ciliophora (4), Dinophyta (3), Cryptophyta (1), Arthropoda (19) in the Namhae samples). Therefore, the molecular monitoring method developed here can provide additional information regarding the biodiversity and community structure of eukaryotic plankton in environmental samples and helps construct a useful database of biodiversity for aquatic ecosystems.

New records of three lecanid rotifers(Rotifera: Monogononta: Lecanidae) from Korea

  • Yang, Hee-Min;Min, Gi-Sik
    • Journal of Species Research
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    • v.10 no.3
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    • pp.262-266
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    • 2021
  • In this study, three lecanid rotifer species were newly recorded in Korea: Lecane inermis (Bryce, 1892), L. furcata (Murray, 1913), and L. nana (Murray, 1913). Most of the lecanid rotifers recorded in Korea have been collected from stagnant water, and Lecane furcata and L. nana were collected from a reservoir. In contrast, L. inermis was found in a soil sample. Lecane inermis is the first illoricated lecanid species reported in Korea. This species is distinguished from other illoricated species by its toes with relatively long claws. Lecane furcata is the most common lecanid rotifer in submerged vegetation. The morphological characteristics of its lorica are similar to many other lecanid rotifers, but it can be distinguished by its completely fused toes with a terminal fissure, an absence of antero-lateral spines, and the ratio of lorica/toe length. Lecane nana is most similar to L. tryphema Harring & Myers, 1926, but can be distinguished by the toe tips curved outwards. The three species in this study are considered to have a cosmopolitan distribution and have been recorded in many Asian countries. In this paper, we describe the morphological characteristics of the three lecanid species with trophi structures observed by scanning electron microscope.

Differences in Zooplankton Community Structure between Surface Water and Vertical Integrated Water in Middle and Down Stream of Nakdong River (낙동강 중⋅하류에서 표층 시료와 수직 예망 시료의 동물플랑크톤 군집 구조 차이 비교)

  • Min-Seok Kim;Hae-Kyung Park
    • Journal of Korean Society on Water Environment
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    • v.39 no.3
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    • pp.215-222
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    • 2023
  • Zooplankton are primary consumers in the food web connecting primary producers and predators such as small fish, playing an important role in energy transfer in aquatic ecosystems. Therefore, it is essential to understand the zooplankton community structure in material cycle and energy flow in aquatic ecosystems. Zooplankton in large rivers with a low flow rate would distribute vertically as in lakes. In this study, we collected zooplankton by surface water filtration and vertical haul method with 64 ㎛-mesh plankton net at three stations (ND-1, ND-2, ND-3) in Nakdong River fortnightly from June 2018 to December 2019. Species composition and individual densities were analyzed. All three stations showed differences in relative abundance of zooplankton groups between surface water and vertical integrated water, with the largest difference shown in the deepest station, ND-2. In vertically integrated water at ND-2, the relative abundance of rotifera was low by a maximum of 25% and that of cladocera was high by a maximum of 22% compared to surface water samples. These results indicate that surface water filtration method is not enough to represent the community structure of zooplankton compared to the vertical haul method in large rivers.

New record of three monogononts(Rotifera: Monogononta) from islands of Korea

  • Hee-Min Yang;Gi-Sik Min
    • Journal of Species Research
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    • v.12 no.2
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    • pp.174-179
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    • 2023
  • Three newly recorded rotifers were collected through surveys of several islands in Korea: Lecane perplexa (Ahlstrom, 1938); Lindia torulosa Dujardin, 1841; and Monommata maculata Harring & Myers, 1930. These species represent 24th, second, and third records of each genus discovered in Korea, respectively. Lecane perplexa and Monommata maculata, were collected from reservoirs using plankton nets, whereas Lindia torulosa was collected from a soil sample. The morphological characteristics of the discovered species are as follows: Lecane perplexa is characterized by a straight or slightly concave anterior margin of the lorica, an incomplete transverse fold on the ventral lorica, and a single toe with pseudoclaws and accessory claws; Lindia torulosa has an illoricated and vermiform body, a head with rostrum, a pair of auricles, cardate type trophi, and distinctively shaped epipharynx components; and Monommata maculata is characterized by long toes, an inner margin of rami with 10-12 serrated teeth, 3-4 large teeth, and interlocking teeth. Here, the morphological characteristics of the three Korean monogonont rotifers, which include trophi photographs, are presented. In addition, partial sequences of the mitochondrial cytochrome c oxidase subunit I (COI) of each species are presented.

Survival of the early lavae of the Freshwater Crab, Eriocheir japonicus (De Haan) fed on different diets in the Laboratory (먹이종류에 따른 동남참게, Eriocheir japonicus(De Haan) 초기유생의 생존율)

  • 허윤성;권진수;이복규;김홍권;김병기;최주수;김양우
    • Journal of Life Science
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    • v.12 no.4
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    • pp.407-415
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    • 2002
  • The survival of the early larvae of the freshwater crab, E. japonicus, fed on different diets at 22土1$^{\circ}C$ and 24$\pm$1% were studied in the laboratory. The larvae in the control (no feed) lived for 4 days (1~2 zoeal stages), and in the experimental container of non-living foods (egg Powder, soy bean Powder, Pellet Powder, millet Powder, and mussel meat), they were lived for 7~9 days (2~3 zoeal stages), When the larvae fed on phytoplankton (Chlorella ellipsoid., Skeletonema costatum. and Chaetoceros gracilis), they lived for 10, 18, and 19 days(3~5 zoeal stages), and fed on zooplankton (Artemianauplii and rotifer), they were reached to the juvenile stage in 24 and 25 days, respectivily. When the larvae fed on a mixed diets (see table 1). it was more effective than a single food diet, and the most effective diets included Chaetoceros gracilis, Artemia nauplii and rotifera, in which the larvae reached the juvenile stage in 22 days, and the survival was 73%.

Past History of Freshwater Zooplankton Research in South Korea and Korean Society of Limnology and Future Directions (한국하천호수학회를 통해 본 국내 담수 동물플랑크톤 연구의 역사와 현재, 미래의 연구 동향)

  • Hyun-Woo, Kim;Jeong, Hyun-Gi;Choi, Jong-Yun;Kim, Seong-Ki;Jeong, Kwang-Seuk;La, Geung-Hwan;Oh, Hye-Ji;Chang, Kwang-Hyeon
    • Korean Journal of Ecology and Environment
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    • v.51 no.1
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    • pp.40-59
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    • 2018
  • This review summarizes the history of freshwater zooplankton research in Korean Society of Limnology and necessary future topics that are remain poorly investigated in South Korea based on main research topics of published articles focusing on lakes, reservoirs, rivers and wetland ecosystems. In Korea, a total 450 freshwater zooplankton species have been reported (85 species of cladocera, ca. 230 species of rotifera since 1939, and 138 species of copepoda), and they cover 10% of total zooplankton species list. In the present paper, we provide recent species list of zooplankton found in Korea and their taxonomic keys. Over periods of 45 years, there are 25 published papers for zooplankton biota in lentic ecosystems in Korean Journal of Ecology and Environment (KJEE). The ecology of zooplankton communities in rivers has focused on the mechanisms involved in regulating their abundance, diversity and spatio-temporal patterns of genus Bosmina and rotifers (genus Brachionus and Keratella) that are most frequently found from Korea. On the other hand, the studies on zooplankton in wetland has focused on Alona, Chydorus and Ceriodaphnia with special emphasis on their relationships with aquatic macrophytes. Even though studies on the freshwater ecosystem in Korea have a long history, a few of studies on zooplankton biota were conducted at rice paddy, Dumbeong and wetlands. During the last two decades, experimental advances in freshwater zooplankton ecology and understanding of structure and function of this biota were made from a series of articles mainly in journal of KJEE. For future studies, quantitative, experimental and interdisciplinary approaches would be key words to understand zooplankton ecology and their roles in aquatic ecosystems under changing environments, and we have suggested necessary zooplankton research fields and future directions.

Analysis of Food Sources of Pre- and Post-diet in a Bivalve Using DNA Metabarcoding (DNA metabarcoding을 이용한 이매패류 공식 전후 먹이원 분석)

  • Bong-Soon Ko;Jae-won Park;Chang Woo Ji;Ihn-Sil Kwak
    • Korean Journal of Ecology and Environment
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    • v.55 no.4
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    • pp.360-367
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    • 2022
  • Research on food sources through DNA metabarcoding is being used for various organisms based on high resolution and reproducibility. In the study, we investigated the difference in food sources between pre and post-starving in the three bivalve species (Anemina acaeformis, Anodonta woodiana, and Unio douglasiae) through DNA metabarcoding using 18S rRNA V9 primer. The food source of pre-starving appeared in 87 genera, 71 families, 51 orders, 35 classes, and 22 phyla. The primary food sources were the zoo and phytoplankton, including Chlamydomonadales, Euglenales, Ploima, Sphaeropleales, and Stephanodiscales. However, all zoo and phytoplankton were not observed after starving except Schizopyrenida and Rotifera. In Levin's niche breadth analysis, the Bi index of A. woodiana is 0.3, which was higher than A. acaeformis(0.14) and U. douglasiae (0.21), indicating that they feed on various food sources. The niche overlap of A. acaeformis was measured as 0.78 in A. woodiana, 0.7 in U. douglasiae showing a relative high value compared to other bivalves. The trophic level of A. acaeformis, A. woodiana, and U. douglasiae based on the food source information were investigated as 2.0, 2.0, and 2.5, respectively. The results of the previous study on the trophic level using stable isotopes showed 1.8 to 2.4 values were similar to the results of this study. These results suggest that DNA metabarcoding can be an effective analyzing tool for the gut content in the bivalves.

Unrecorded species of Korean invertebrates discovered through the project of 'Discovery of Korean Indigenous Species' II

  • Su-Jung Ji;Chuleui Jung;Hyun Woo Bang;Min Ok Song;Jongwoo Jung;Seong Myeong Yoon;Seunghwan Lee;Seoyoung Keum;Hee-Min Yang;Dongmin Lee;Geon Hyeok Lee;Jaeseok Oh;Kichoon Kim;Hansol Park;Heejin Moon;Omid Joharchi;Yeseul Kang;Keeseon S. Eom;Kyung Jin Lee;Ye Eun;Taeho Kim;Ivana Karanovic;Jeounghee Lee;Seongjun Choe;Gi-Sik Min
    • Journal of Species Research
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    • v.12 no.1
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    • pp.68-89
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    • 2023
  • This is the second catalog listing unrecorded invertebrates discovered during the research project 'Discovery of Korean Indigenous Species'. The data to compile the catalog were primarily gathered from the final reports of the project, between 2013 and 2021. We present 38 previously undocumented species, belonging to four phyla (Platyhelminthes, Annelida, Rotifera and Arthropoda). Samples were collected from intertidal coastal waters, soil, freshwater ponds, reservoirs and hosts in South Korea. In this study, we provide brief taxonomic information, including collection site (GPS), diagnosis, specimen vouchers, figures of representative individuals and the Korean name newly assigned, for each species. All data were reviewed and updated by experts working on the respective taxonomic group. The aim of the present study is to publish species that have been previously reported through the project. Upon publication, these species will be added to the 'National Species List of Korea', curated by the National Institute of Biological Resources(NIBR).

Limno-Biological Investigation of Lake Ok-Jeong (옥정호의 육수생물학적 연구)

  • SONG Hyung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.15 no.1
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    • pp.1-25
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    • 1982
  • Limnological study on the physico-chemical properties and biological characteristics of the Lake Ok-Jeong was made from May 1980 to August 1981. For the planktonic organisms in the lake, species composition, seasonal change and diurnal vertical distribution based on the monthly plankton samples were investigated in conjunction with the physico-chemical properties of the body of water in the lake. Analysis of temperature revealed that there were three distinctive periods in terms of vertical mixing of the water column. During the winter season (November-March) the vertical column was completely mixed, and no temperature gradient was observed. In February temperature of the whole column from the surface to the bottom was $3.5^{\circ}C$, which was the minimum value. With seasonal warming in spring, surface water forms thermoclines at the depth of 0-10 m from April to June. In summer (July-October) the surface mixing layer was deepened to form a strong thermocline at the depth of 15-25 m. At this time surface water reached up to $28.2^{\circ}C$ in August, accompanied by a significant increase in the temperature of bottom layer. Maximum bottom temperature was $r5^{\circ}C$ which occurred in September, thus showing that this lake keeps a significant turbulence Aehgh the hypolimnial layer. As autumn cooling proceeded summer stratification was destroyed from the end of October resulting in vertical mixing. In surface layer seasonal changes of pH were within the range from 6.8 in January to 9.0 in guutuost. Thighest value observed in August was mainly due to the photosynthetic activity of the phytoplankton. In the surface layer DO was always saturated throughout the year. Particularly in winter (January-April) the surface water was oversaturated (Max. 15.2 ppm in March). Vertical variation of DO was not remarkable, and bottom water was fairly well oxygenated. Transparency was closely related to the phytoplankton bloom. The highest value (4.6 m) was recorded in February when the primary production was low. During summer transparency decreased hand the lowest value (0.9 m) was recorded in August. It is mainly due to the dense blooming of gnabaena spiroides var. crassa in the surface layer. A. The amount of inorganic matters (Ca, Mg, Fe) reveals that Lake Ok-Jeong is classified as a soft-water lake. The amount of Cl, $NO_3-N$ and COD in 1981 was slightly higher than those in 1980. Heavy metals (Zn, Cu, Pb, Cd and Hg) were not detectable throughout the study period. During the study period 107 species of planktonic organisms representing 72 genera were identified. They include 12 species of Cyanophyta, 19 species of Bacillariophyta, 23 species of Chlorophyta, 14 species of Protozoa, 29 species of Rotifera, 4 species of Cladocera and 6 species of Copepoda. Bimodal blooming of phytoplankton was observed. A large blooming ($1,504\times10^3\;cells/l$ in October) was observed from July to October; a small blooming was present ($236\times10^3\;cells/l$ in February) from January to April. The dominant phytoplankton species include Melosira granulata, Anabaena spiroides, Asterionella gracillima and Microcystis aeruginota, which were classified into three seasonal groups : summer group, winter group and the whole year group. The sumner group includes Melosira granulate and Anabaena spiroides ; the winter group includes Asterionella gracillima and Synedra acus, S. ulna: the whole year group includes Microtystis aeruginosa and Ankistrodesmus falcatus. It is noted that M. granulate tends to aggregate in the bottom layer from January to August. The dominant zooplankters were Thermocpclops taihokuensis, Difflugia corona, Bosmina longirostris, Bosminopsis deitersi, Keratelle quadrata and Asplanchna priodonta. A single peak of zooplankton growth was observed and maximum zooplankton occurrence was present in July. Diurnal vertical migration was revealed by Microcystis aeruginosa, M. incerta, Anabaena spiroides, Melosira granulata, and Bosmina longirostris. Of these, M. granulata descends to the bottom and forms aggregation after sunset. B. longirostris shows fairly typical nocturnal migration. They ascends to the surface after sunset and disperse in the whole water column during night. Foully one species of fish representing 31 genera were collected. Of these 13 species including Pseudoperilnmpus uyekii and Coreoleuciscus splendidus were indigenous species of Korean inland waters. The indicator species of water quality determination include Microcystis aeruginosa, Melosira granulata, Asterionelta gracillima, Brachionus calyciflorus, Filinia longiseta, Conochiloides natans, Asplanchna priodonta, Difflugia corona, Eudorina elegans, Ceratium hirundinella, Bosmina longirostris, Bosminopsis deitersi, Heliodiaptomus kikuchii and Thermocyclops taihokuensis. These species have been known the indicator groups which are commonly found in the eutrophic lakes. Based on these planktonic indicators Lake Ok-Jeong can be classified into an eutrophic lake.

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