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http://dx.doi.org/10.11614/KSL.2022.55.4.360

Analysis of Food Sources of Pre- and Post-diet in a Bivalve Using DNA Metabarcoding  

Bong-Soon Ko (Department of Ocean Integrated Science, Chonnam National University)
Jae-won Park (Department of Ocean Integrated Science, Chonnam National University)
Chang Woo Ji (Fisheries Science Institute, Chonnam National University)
Ihn-Sil Kwak (Department of Ocean Integrated Science, Chonnam National University)
Publication Information
Korean Journal of Ecology and Environment / v.55, no.4, 2022 , pp. 360-367 More about this Journal
Abstract
Research on food sources through DNA metabarcoding is being used for various organisms based on high resolution and reproducibility. In the study, we investigated the difference in food sources between pre and post-starving in the three bivalve species (Anemina acaeformis, Anodonta woodiana, and Unio douglasiae) through DNA metabarcoding using 18S rRNA V9 primer. The food source of pre-starving appeared in 87 genera, 71 families, 51 orders, 35 classes, and 22 phyla. The primary food sources were the zoo and phytoplankton, including Chlamydomonadales, Euglenales, Ploima, Sphaeropleales, and Stephanodiscales. However, all zoo and phytoplankton were not observed after starving except Schizopyrenida and Rotifera. In Levin's niche breadth analysis, the Bi index of A. woodiana is 0.3, which was higher than A. acaeformis(0.14) and U. douglasiae (0.21), indicating that they feed on various food sources. The niche overlap of A. acaeformis was measured as 0.78 in A. woodiana, 0.7 in U. douglasiae showing a relative high value compared to other bivalves. The trophic level of A. acaeformis, A. woodiana, and U. douglasiae based on the food source information were investigated as 2.0, 2.0, and 2.5, respectively. The results of the previous study on the trophic level using stable isotopes showed 1.8 to 2.4 values were similar to the results of this study. These results suggest that DNA metabarcoding can be an effective analyzing tool for the gut content in the bivalves.
Keywords
food source; gut content; bivalve; DNA metabarcoding; 18S rRNA primer;
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Times Cited By KSCI : 6  (Citation Analysis)
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