• Current Research on Agriculture and Life Sciences
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• 제31권3호
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• pp.170-176
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• 2013

In this study, we selected some material to have potential bioactivity from natural plants, confirmed as basic data for industrializing and tried to develope the food materials using them. DPPH, ABTS, antioxidant protection factor, TBARs and antimicrobial activity of extracts from Rosa multiflora Thunberg fruits were determined. The total phenolics extracted from Rosa multiflora were 12.08, 11.82, 11.1 and 12.6 mg/g when using water, 70% ethanol, 70% methanol and 70% acetone as the solvent, respectively. The optimum conditions for extracting the phenolic compounds were 70% ethanol over for 12 hrs(11.82 mg/g). The electron donating ability and inhibition rate on ABTS of the 70% ethanol extracts were 97% and 92.2%, respectively while the antioxidant protection factor(PF) of the water extracts and 70% ethanol extracts were 1.79 and 1.34 PF, respectively. The TBAR (thiobarbituric acid reactive substance) value were $1.3{\mu}M$ for the control and $0.15{\mu}M$ for the 70% ethanol extracts. The inhibitory activity against ${\alpha}$-amylase was 26% for the 70% ethanol extracts. The 70% ethanol extracts from Rosa multiflora Thunberg fruits exhibited antimicrobial activity against H. pylori, S. epidermidis, S. aureusand and E. coli with clear zone diameters of 14, 25, 14 and 13 mm, respectively when using $200{\mu}g/mL$ of the phenolic compounds. An HPLC analysis identified 6 major phenolic metabolites in the Rosa multiflora Thunberg fruits extracts: rosmarinic acid, caffeic acid, chlorogenic acid, courmaric acid, protocatechuic acid and quercetin. In particular, the content of rosmarinic acid was the highest in the 70% ethanol extracts. Therefore these results indicate that 70% ethanol extracts from Rosa multiflora Thunberg fruits can be useful as a natural antioxidant and in functional foods.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.364-364
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• 2005

• 한국식품과학회지
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• 제44권1호
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• pp.34-40
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• 2012

본 연구는 고기능성 들깻잎을 사용한 침출차 제조의 품질 극대화를 위한 최적볶음조건을 설정하기 위하여 볶음조건을 볶음온도($120-200^{\circ}C$)와 볶음시간(15-35 min)을 달리하면서 들깻잎 차의 항산화력과 생리활성성분을 분석하여 들깻잎의 품질변화를 모니터링하고 실험구간 내 볶음조건 별 종합적인 품질비교를 통하여 최적 볶음조건을 선정하였다. 볶음조건에 따른 페놀성 화합물 함량은 대체적으로 $160^{\circ}C$ 이하에서는 시간이 증가할수록 함량이 증가하였고 $160^{\circ}C$ 이상에서는 온도가 상승할수록 함량이 증가하는 경향을 보였다. 페놀성 화합물 함량이 높은 볶음조건에서 자유라디칼 소거능 및 환원력 모두 우수한 항산화활성을 나타내었다. Caffeic acid 및 rosmarinic acid는 실험구간 내 낮은 온도에서는 시간이 증가할수록 함량이 증가하다가 대체적으로 온도가 상승할수록 감소하였다. 실험구간 내 볶음조건별 들깻잎의 품질 특성을 종합적으로 비교 분석한 결과, 고기능성 들깻잎을 이용한 침출차의 품질을 극대화하는 최적 볶음조건은 $180^{\circ}C$, 20 min로 선정되었다.

• Biomolecules & Therapeutics
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• 제25권2호
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• pp.105-111
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• 2017

It has been known that RA, one of major constituents of Perilla frutescens which has been used as a traditional folk remedy for sedation in oriental countries, shows the anxiolytic-like and sedative effects. This study was performed to know whether RA may enhance pentobarbital-induced sleep through ${\gamma}-aminobutyric$ acid $(GABA)_A-ergic$ systems in rodents. RA (0.5, 1.0 and 2.0 mg/kg, p.o.) reduced the locomotor activity in mice. RA decreased sleep latency and increased the total sleep time in pentobarbital (42 mg/kg, i.p.)-induced sleeping mice. RA also increased sleeping time and number of falling sleep mice after treatment with sub-hypnotic pentobarbital (28 mg/kg, i.p.). In electroencephalogram (EEG) recording, RA (2.0 mg/kg) not only decreased the counts of sleep/wake cycles and REM sleep, but also increased the total and NREM sleep in rats. The power density of NREM sleep showed the increase in ${\delta}-waves$ and the decrease in ${\alpha}-waves$. On the other hand, RA (0.1, 1.0 and $10{\mu}g/ml$) increased intracellular $Cl^-$ influx in the primary cultured hypothalamic cells of rats. RA (p.o.) increased the protein expression of glutamic acid decarboxylase ($GAD_{65/67}$) and $GABA_A$ receptors subunits except ${\beta}1$ subunit. In conclusion, RA augmented pentobarbital-induced sleeping behaviors through $GABA_A-ergic$ transmission. Thus, it is suggested that RA may be useful for the treatment of insomnia.

• 대한한약학회:학술대회논문집
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• 대한한약학회 2007년도 Autumn Conference
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• pp.181-181
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• 2007

• 생물환경조절학회지
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• 제27권3호
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• pp.260-268
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• 2018

본 연구는 수경재배 방식으로 재배 된 배초향(Agastache rugosa)의 생장과 항산화 물질 축적에 대하여 단색 또는 복합 LEDs의 광질이 미치는 영향을 살펴 보고자 수행하였다. 본엽 4매인 배초향 묘를 수경재배 시스템에 정식하였으며, 백색(W10), 적색(R10), 혼합 LEDs (W2B1G1, R3B1, R2B1G1, W2B1G1: 각 LED의 PPFD 비율) 및 형광등(FL: 대조구)을 이용하여 $180{\pm}5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$의 광합성광량자속밀도(PPFD)의 조건과, 16/8h 광주기, $22{\pm}1/18{\pm}1^{\circ}C$ (주/야간) 온도, $60{\pm}10%$의 상대 습도로 관리된 재배실에서 4주동안 배초향을 재배하였다. W2B1G1 광조건에서 자란 배초향의 생체중과 건물중은 모든 처리중에서 가장 큰 값을 나타내었다. R10 조건에서 재배 된 배초향은 초장이 가장 길었지만 SPAD는 모든 처리와 비교해서 가장 낮은 값을 보였다. 배초향의 건물중당 rosmarinic acid 농도는 W2B1G1 처리구에서 유의하게 높았다. 건물당 tilianin 함량은 다른 처리구들과 비교할 때, R3B1에서 유의적으로 가장 높았다. 그러나 전체 식물체에 함유 된 rosmarinic acid와 tilianin 함량은 W2B1G1 조건에서 가장 높았다. 식물공장에서 배초향을 재배하기 위해, W2B1G1으로 구성된 혼합 LED 광 조건이 배초향의 생장과 항산화 물질축적에 가장 유리한 것으로 나타났다. 본 연구는 식물 전체에 함유 된 항산화 물질의 총량이 상업적 용도로 중요하다는 것과, 광질의 최적 선택을 통해서 기능성물질의 증대가 가능하다는 것을 보여주었다.

• 한국자원식물학회지
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• 제33권1호
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• pp.40-49
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• 2020

The objectives of this study were to evaluate total phenolic content (TPC) and individual phenolic compounds in leaves of perilla genetic resources, assess whether they could be used as distinguishing factor among germplasms, and evaluate their relationship with some quantitative and qualitative morphological characters. TPC and individual phenolic compounds were determined using Folin-Ciocalteu method and UPLC-PDA system, respectively. Wide variations in TPC (7.99 to 133.70 mgGAE/g DE), rosmarinic acid (ND to 21.05 mg/g DE), caffeic acid (ND to 1.17 mg/g DE), apigenin-7-O-diglucuronide (ND to 2.21 mg luteolin equivalent (mgLUE)/g DE), scutellarein-7-O-glucuronide (ND to 5.25 mg LUE/g DE), and apigenin-7-O-glucuronide (ND to 2.81 mg LUE/g DE) were observed. Intensities of green pigment at abaxial and adaxial leaf surfaces were positively correlated with phenolic compounds whereas leaf length and width had negative correlation. Purple pigmented accessions were shorter in leaf length and width but exhibited higher amount of phenolic compounds compared to green pigmented accessions in most cases. Leaf shape was not related with content of phenolic compounds, color of leaves, and length/width of leaves. TPC and individual phenolic compounds along with morphological characters could be useful distinguishing factors for perilla genetic resources.

• Journal of Plant Biotechnology
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• 제3권2호
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• pp.107-112
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• 2001

The kinetics of Ti-transformed Salvia miltiorrhiza cell cultures was studied in 250-$m\ell$ shake flasks by using B5 medium with addition of 30 gfL of sucrose. In the cell cultures, the maximum cell mass obtained was 11.5 g DW/L on day 15. The highest amount of phenolic compounds - rosmarinic acid (RA) and lithospermic acid B (LAB) reached 871.3 mg/L (day 15) and 121.3 mg/L (day 13), respectively. The total tanshinone production, i.e., intracellular plus extracellular cryptotanshinone, tanshinone 1, and tanshinone IIA, was 5.3 mg/L on day 13. For the cultivations in 2.4-L stirred bioreactors, the residual sugar level and medium conductivity were a little higher in a small turbine impeller reactor ($T_s$) than those in a large turbine impeller reactor ($T_L$), while a higher cell density was obtained in the $T_L$. For the production of tanshinones and phenolics, better results were obtained in the $T_L$ than in the $T_s$. In the $T_L$, similar or even a little higher production titers of tanshinones and phenolic compounds were achieved compared to those in the flasks. The results suggest that the shake flask results could be successfully scaled up to the $T_L$ reactor. Such a large impeller reactor like $T_L$ may be better than a small impeller one for the large-scale production of the valuable metabolites by the suspension cultures of Ti transformed S.miltiorrhiza cells. This is considered due to the beneficial culture environment in the $T_L$, such as low shear rates as estimated theoretically.

• 한국응용과학기술학회지
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• 제33권4호
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• pp.647-657
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• 2016

본 연구에서는 하고초(Prunella vulgaris L.)의 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획을 대상으로 항산화 활성과 유효성분 분석 연구를 수행하였다. 1,1-Diphenyl-2-picrylhydrazyl (DPPH)를 이용한 자유라디칼 소거활성($FSC_{50}$)은 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획에서 각각 $15.25{\mu}g/mL$, $8.68{\mu}g/mL$ 및 $8.25{\mu}g/mL$이었다. Luminol 발광법을 이용한 $Fe^{3+}-EDTA/H_2O_2$계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 하고초 추출물의 총항산화능($OSC_{50}$)은 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획에서 각각 $4.68{\mu}g/mL$, $1.00{\mu}g/mL$ 및 $1.02{\mu}g/mL$이었다. $^1O_2$로 유도된 적혈구 세포손상에 대한 보호효과는 $1{\sim}25{\mu}g/mL$의 농도에서 농도 의존적으로 증가하였고, 특히 $25{\mu}g/mL$ 농도에서 아글리콘 분획의 ${\tau}_{50}$이 337.9 min으로 높은 세포 보호 활성을 나타내었다. 이는 대표적인 항산화 물질로 알려진 (+)-${\alpha}$-tocopherol (${\tau}_{50}=38.7$ 분)보다도 9배나 큰 세포 보호 효과를 나타냄을 알았다. 실험에 사용된 하고 초 추출물의 에틸아세테이트 분획과 아글리콘 분획에 대하여 TLC와 HPLC를 이용한 성분 분석을 수행하였다. 에틸아세테이트 분획에서는 caffeic acid, rosmarinic acid, quercetin 3-${\beta}$-D-glucoside, rutin, kaempferol-3-O-rutinoside, astragalin (kaempferol-3-O-glucoside)을 확인하였다. 아글리콘 분획에서는 caffeic acid, rosmarinic acid, quercetin 및 kaempferol이 존재함을 확인하였다. 이상의 결과들로부터, 하고초 추출물 및 분획물은 $^1O_2$ 또는 ROS를 소거함으로써 세포막을 보호할 수 있는 천연 항산화제로서 화장품 분야에서 응용이 가능함을 시사하였다.

• 분석과학
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• 제32권3호
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• pp.96-104
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• 2019

Agastache rugosa, known as Korean mint, is a medicinal plant with many beneficial health effects. In this study, a simple and reliable HPLC-UV method was proposed for the quantification of rosmarinic acid (RA) in the aqueous extracts of A. rugosa. RA was selected as a quantification marker due to its easiness in procurement and analysis. The developed method involved chromatographic separation on a $C_{18}$ column ($250{\times}4.6mm$, $5{\mu}m$) at room temperature. The mobile phase consisted of water and acetonitrile both containing 2 % acetic acid and was run at a flow rate of $1mL\;min^{-1}$. The method was validated for specificity, linearity, precision, and accuracy. It was specific to RA and linear in the range of $50-300{\mu}g\;mL^{-1}$ ($r^2=0.9994$). Intra-day, inter-day, and inter-analyst precisions were ${\leq}0.91%\;RSD$, ${\leq}1.40%\;RSD$, and 1.94 % RSD, respectively. Accuracy was 93.3-95.9 % (${\leq}1.21%\;RSD$). The method could be applied to three batches of bulk samples and three batches of lab scale samples, which were found to be $0.64({\pm}0.04)mg\;g^{-1}$ and $0.48({\pm}0.02)mg\;g^{-1}$ for the dried raw materials of A. rugosa. The results show that the proposed method can be used as a readily applicable method for QC of health functional foods containing the aqueous extracts of A. rugosa.