• Agricultural and Biosystems Engineering
• /
• v.5 no.2
• /
• pp.55-59
• /
• 2004

This study aimed to develop a seeder for sowing large seeds such as those of fruit vegetables used as rootstocks in grafting. It investigated how the nozzle diameter and the nozzle's vacuum pressure affected the seeding performance. This study found that a nozzle diameter of 1.5 mm was capable of sowing most of the seed sizes used in this study. The highest seeding rates for 'Chambak', 'Tuktozwa', and 'Hukjong' were $97.6\%$, $98.8\%$, and $97.6\%$, respectively. Lineup status after sowing was good. The working performance was 75.6 sheets/hr. An average seeding rate of 1 grain was $97.8\%$.

• Korean Journal of Organic Agriculture
• /
• v.19 no.spc
• /
• pp.267-270
• /
• 2011

Root-knot nematodes cause a significant damage on fruit yield and quality of green house growing crops. To asses the effect of eggplant rootstock, Torvum vigor', TaibyouVF' and 'Daitaro' were grafted on eggplants(Solanum melongena cv. Chookyang) and planted in root-knot nematode infested microplot in green house and compared their fruit yield, quality and plant growth with non-grafted control. Eggplant grafted with Torvum vigor had the highest fruit yield and top growth and followed by Daitaro. Non-grafted eggplant had lower yield but had higher root weight because of heavy root-knot nematode infection. Rootstock grafting in eggplant farming is a good alternative technique in root-knot nematode infested green houses without compromising fruit yield and can be applied instantly as organic farming practice.

• Journal of Bio-Environment Control
• /
• v.19 no.4
• /
• pp.324-332
• /
• 2010

This experiment was carrid out to develop a culture method by sequential highland-lowland cultivation for year-round production of fresh leaves in Ligularia stenocephala. Rootstocks of Ligularia stenocephala cultivated in Daegwallyeong openfield were digged up on late October 2007 and then stored in $-2^{\circ}C$ refrigerator. The rootstocks were monthly transplanted in protected greenhouse in Gangneung from November 2007 to June 2008 for lowland cultivation, and also they were monthly transplanted in rainshelter in Daegwallyeong from June to August 2008 for highland cultivation. The early growth of Ligularia stenocephala transplanted on January 2008 was faster. Most plant of Ligularia stenocephala regardless of transplanting time of rootstock grew over 30cm at the time of first harvesting of leaves till May, while the yield decreased during the summer season. The average plant height of Ligularia stenocephala transplanted on July and August in Daegwallyeong highland was lower than that cultivated in Gangneung lowland during spring season. Using of rootstock stored made it possible to produce in highland from Apr. to Oct. during summer season, and also to produce in lowland from Nov. to May next year. Therefore, the results indicates that sequential highland-lowland cutivation by using of stored rootstock will be capable of yearround production of fresh leaves in Ligularia stenocephala.

• Journal of Bio-Environment Control
• /
• v.22 no.2
• /
• pp.146-153
• /
• 2013

This study was conducted to enhance graft-take ratio and quality of grafted tomato seedlings by controlling temperature and humidity during the healing and acclimatization processes. Three temperature levels ($20^{\circ}C$, $23^{\circ}C$, and $26^{\circ}C$) were carried out to determine optimum temperature on four rootstocks. In addition, twelve combinations of three relative humidity levels (70%, 80%, and 90%) and four temperature levels ($17^{\circ}C$, $20^{\circ}C$, $23^{\circ}C$, and $26^{\circ}C$) were set up to evaluate the effect of relative humidity and temperature on the graft-take ratio of grafted seedlings. In the other hand, five relative humidity periods (H0, H1, H2, H3, and H4: 90% relative humidity for first 0, 1, 2, 3 and 10 days and afterwards relative humidity was reduced to 70%, respectively) were examined effect of relative humidity periods on the graft-take and quality of grafted seedlings. The higher graft-take ratios (84.0~87.4%) were showed at $23^{\circ}C$ compared to $20^{\circ}C$ and $26^{\circ}C$ in all rootstocks. Graft-take ratios decreased and number of diseased plants increased at high temperature. The graft-take ratios increased with increasing relative humidity in all temperature levels on the $3^{rd}$ and $7^{th}$ day after grafting. However, increasing relative humidity significantly increased percent of diseased plants. The graft-take ratio reduced at ($26^{\circ}C$) and ($17^{\circ}C$) temperature under all relative humidity conditions. The graft-take ratio increased with increasing period of 90% relative humidity. Maximum graft-take ratios were observed in H2 and H3 treatments. Graft-take ratio decreased with increasing 90% relative humidity for 10 days (H4). Diseased plants had not been found in H0, H1, H2, and H3 treatments. Seedling quality was improved through increasing fresh and dry weight of root, compactness, and root morphology of tomato seedlings in H2 and H3 treatments. Therefore, high relative humidity (90%) for first 2 or 3 days and afterwards reduced low relative humidity (70%) at $23^{\circ}C$ condition during healing and acclimatization promoted the graft-take and quality of grafted tomato seedlings.

• Journal of Plant Biotechnology
• /
• v.46 no.3
• /
• pp.228-235
• /
• 2019

Apple (Malus pumila) is one of the most economically important fruits in Korea. but virus infection has decreased the sustainable production of apples and caused serious problems such as yield loss and poor fruit quality. Virus or viroid infection including apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple mosaic virus (ApMV) and apple scar skin viroid (ASSVd) have been also reported in Korea. In many cases, as apple gets infected with virus and viroid with no specific symptoms, the damage and symptoms caused by the viruses are not detected. In our research, viruses in the rootstock were eliminated for a virus-free apple dwarfing rootstock of M.9 and M.26. The virus elimination methods were apical meristem culture, thermotherapy ($37^{\circ}C$, 6 weeks) and chemotherapy($Ribavirin^{(R)}$). The detection of apple viruses was accomplished by Enzyme-linked Immuno-Sorbent Assay (ELlSA) and reverse transcription-polymerase chain reaction (RT-PCR). RT- PCR method was 10 ~ 30% more sensitive than the ELISA method. The efficiency of virus elimination was enhanced in apical meristem culture method. The acquisition rate of virus-free apple dwarfing rootstocks was 30 ~ 40% higher in apical meristem culture. After the meristem culturing of M.9, the infection ratio of ACLSV, ASPV and ASGV was 45%, 60% and 50%, respectively. In the apple dwarfing rootstock of M.26, the infection ratio of ACLSV, ASPV and ASGV was 40%, 55% and 55%, respectively. Based on this study, the best method for the production of virus-free apple dwarfing rootstocks was the apical meristem culture.

• Horticultural Science & Technology
• /
• v.31 no.3
• /
• pp.371-379
• /
• 2013

Anthracnose disease caused by Colletotrichum orbiculare, induces severe damage to cucurbits worldwide. Resistance of 112 commercial cultivars of cucurbits to C. orbiculare was evaluated. Seedlings of each cultivar at 2- to 3-leaf stage were inoculated with C. orbiculare KACC 40809 by spraying spore suspension of the fungus at a concentration of $4.0{\times}10^5$ spores/mL. Among the 36 cultivars of cucumber, 'Asiastrike', 'Tongilbaedadagi', 'Daeseon', 'Cheongrokmatjjang', 'Nokyacheongcheong', and 'Asianogak' were moderately resistant and the others were susceptible. All the tested cultivars of melon (33) and watermelon (4) showed highly susceptible response to C. orbiculare. On the other hand, the squash cultivars (17) represented less susceptibility to the fungus than the other cucurbits. Of the squash cultivars, 'Gammirak' and 'Teotbat' were resistant and 12 cultivars were moderately resistant. Among the rootstocks for cucurbits, ten cultivars including 'JjeuyakaEX', 'Nunbusyeo', 'Union', 'RS111', 'Ganggeuntoza', 'Hwangjaetoza', 'NO.8', 'Shintoza', 'Bulpaetoza', and 'Newtype' showed high resistance to the anthracnose pathogen. From the results, the resistant cultivars could be used as sources of resistance to cucurbits anthracnose (C. orbiculare) in the future breeding programs.

• Journal of Bio-Environment Control
• /
• v.20 no.3
• /
• pp.204-210
• /
• 2011

This experiment was conducted to investigate the effect of water states (water, osmotic potential and turgor pressure) of fruit vesicle and leaf on soluble solids and organic acid contents of fruits of 'Shiranuhi' mandarin hybrid, M16 A line during the fruit maturing season in plastic house cultivation. The 'Shirauhi' grafted on 'Swingle citrumelo', strong strength of rootstock, produced fruit with lower soluble solids and organic acid content than 'Trifoliate' orange rootstock. The fruits vesicle water potential and turgor pressure measured before dawn in 'Swingle citrumelo' were higher tendency than the 'Trifoliate' orange, but osmotic potential values were lower than the 'Trifoliate' orange. The changes of leaf water potential were very similar to the fruit. The results suggest that in the 'Shirauhi' fruits grafted on two rootstocks changes of soluble solids and organic acid content of the fruit were influenced by the leaf water potential and the osmotic potential of the fruit vesicles, which might be caused by the difference of root distribution between two rootstocks.

• Journal of Bio-Environment Control
• /
• v.25 no.4
• /
• pp.302-307
• /
• 2016

The aim of this study was to examine the optimum wattage and installation height using nano-carbon fiber infrared heating lamp (NCFIHL) for heating energy saving and plug seedling production in plug seedling production greenhouse in winter season. NCFIHL of 700 and 900 W was installed over the bed ($1.2{\times}2.4m$) as 0.7, 1.0, and 1.3 m height, respectively, for the production of grafted watermelon seedling in venlo-type glasshouse. Watermelon (Citrullus lanatus (Thunb.) Manst.) 'Jijonggul' and gourd (Lagenaria leucantha Rusby.) 'Sunbongjang' were used as scions and rootstocks, respectively. The scions and rootstocks were grafted by single cotyledon ordinary splice grafting. Light intensity of NCFIHL was below the $1{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ in all treatment. Spectral distributions of NCFIHL presented mostly infrared area. When outside air temperature was below $10^{\circ}C$, 700 and 900 W NCFIHL installed with 0.7 m height treatment and 900 W NCFIHL installed with 1.0 m height treatment maintained the setting air temperature ($20^{\circ}C$) at night. In the result of taking thermal imaging, the grafted watermelons were getting warm fast in 900 W NCFIHL installed with 0.7 m height treatment at night. Compactness of the grafted watermelons was the greatest in 700 W NCFIHL installed with 1.3 m height treatment. The results indicate that NCFIHL installed above 1.0 m height using 700 W was suitable for production of plug seedling.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.13 no.1
• /
• pp.1-9
• /
• 2011

This study was conducted to investigate the influence of different polyethylene film (P.E. film) for mulching on the changes in soil temperature and the production of good feathered apple nursery trees. M.9 rootstocks with stem diameter of 9.1~11.0 mm were planted in plots covered with different P.E. film (i.e., transparent P.E. film, black P.E. film, and bare soil as control). Three weeks after planting, the rootstocks were veneer grafted with 'Sinano Sweet' apple cultivar. In the middle of June, BA was sprayed to nursery for inducing feathering during the growing season. The soil temperature of the control was higher than air temperature by about $0.7^{\circ}C$ from mid April to early October, and that of P.E. film mulching was about $1{\sim}5^{\circ}C$ higher than that of the control. The soil temperature under transparent P.E. film was about $2{\sim}3^{\circ}C$ higher than that under black P.E. film. The diurnal range of soil temperature under the black P. E. film was lowest among all treatments. The P.E. film mulching induced better tree growth and feathering than bare soil. Percentage of good feathering apple nursery of black P.E. film was highest among all treatments because the soil temperature unuder black P.E. film in the early growing season was higher than that of the control and the number of days when the maximum soil temperature was over $35^{\circ}C$ in the summer was lower than that under the transparent P.E. film.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.22 no.1
• /
• pp.27-33
• /
• 2020

To date, various kiwifruit cultivars have been developed and released. Asexual propagation by grafting onto seedlings rootstock have been widely utilized commercially in fruit trees including kiwifruit (Actinidia spp.). Most of commercial kiwifruit cultivars belong to A. chinensis and A. deliciosa. Recently, several cultivars were developed by interspecific hybridization with different species including A. arguta. There is a possibility for graft-incompatibility due to interspecific hybridization and therefore, we investigated graft-compatibility of newly released yellow-fleshed kiwifruit cultivars and a hybrid cultivar with various rootstocks of different species. The yellow-fleshed cultivars grafted onto same species, A. chinensis 'Kuimi', showed good shoot elongation, about 1 m in length 50 days after grafting. In contrast, the shoot elongation of the yellow-fleshed kiwifruit grafted onto different species was retarded. The shoot length of a hybrid cultivar (A. arguta × A. deliciosa) 'Skinny Green' grafted onto four different species was about 1 m in length, showing good graft compatibility with other species. Nevertheless, long term studies for graft compatibility of hybrid cultivars are still needed since graft-incompatibility between different species can occur several years later.