• Journal of Oral Medicine and Pain
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• v.17 no.2
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• pp.119-128
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• 1992

In order to evaluate the relationship of age with the developmental stage on mandibular second molars in female, the author examined the radiographs in standard films taken by intraoral paralleling technic and analysed the development of 620 roots of 310 females ranged from 10 to 18 years. The development was divided into 7 stages : Crown complete (Cr.C.), Root lengthed 1/4(R.1/4), Root length 1/2 (R.1/2), Rooth length 3/4 (R.3/4), Rooth length complete (R.C), Apex 1.2 closed (A. 1/2), Apical closure complete (A.C) The obtained results were as follows : 1. The formation of roots in fulllength of mandibular second molars was completed at 14.21-year-age in mesial root and at 14.96-year-age in distal root. 2. The formation of apical foramen of mandibular second molars was completed at 17.11-year-age in mesial root and at 17.53-year-age in distal root. 3. The mesial root of mandibular second molar was generally developed earlier than distal root (p<0.01) 4. Coefficients of correlation and regression equations between for age(Y) and the developmental stage of mandibular second molar(X) were "Y=1.53X+6.54, r=0.91 (p<0.01)" in mesial root and "Y=1.52X+7.11, r=0.92(p<0.01)" in distal root respectively.l root respectively.

• Journal of Ginseng Research
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• v.13 no.2
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• pp.147-152
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• 1989

The statistical characteristics of three year old American ginseng (Panax quinquefolium 1.) root yields from Lytton, British Columbia, Canada are presented. Ginseng root yield is related to plant density, with the highest yields generally obtained from the sites with the highest plant densities. However, these higher yields are made up of a larger proportion of smaller roots while the proportion of larger roots remains almost constant throughout the range of plant densities sampled. Further, it is suggested that relatively small samples can provide significant insight into crop performance and growth.

• Journal of Ginseng Research
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• v.13 no.1
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• pp.14-18
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• 1989

The graminicides fluazifop-p-butyl and sethoxydim effectively controlled annual grass weeds in 1,2 and year old ginseng crops during a 2 year study. Ginseng plants in the graminicide treated plots showed an early senescence of the shoots ; and at harvest the average root weight (g/root) and yield (kg/m) were slightly lower in these plants than in the untreated check plots. Roots of the graminicide treated plots remained viable to regenerate shoots. The greenhouse study indicated that shoot growth and seed development in ginseng plants were more sensitive to the graminicide treatment than the roots.

• Journal of Ginseng Research
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• v.21 no.3
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• pp.187-195
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• 1997

The Chinese ginseng roots were collected at twelve places of Jilin Province and two places of Liaoning Province in China and their appearances were compared with those of the Korean ginseng roots. The color of the most of the Chinese red ginseng was brown or dark brown and the color of many of the Chinese dried ginseng was pale yellow and the root-age of the most of the Chinese red ginseng as well as the Chinese dried ginseng was evaluated five or six year regardless of the collection places, so it cannot be easily concluded that the color and the root-age of the Chinese ginseng roots are different from those of the Korean ginseng roots. However the rhizomes and the lateral roots of the Chinese ginseng roots were poorly developed and many of them did not have either rhizome or lateral roots. Moreover the rhizomes of the Chinese red ginseng as well as the Chinese dried ginseng were much more easily removed than those of the Korean red ginseng and the Korean white ginseng. Therefore it is thought that the development status of the rhizome and the lateral roots of the Chinese ginseng roots are quite different from those of the Korean ginseng roots.

• Korean Journal of Plant Resources
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• v.9 no.2
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• pp.197-201
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• 1996

To greatly decrease bolting rate and deterioration of root quality, seeds of Angelica gigas had sown and grown several month earlier than traditional seedtime. Roots were harvested in the middle of November in same year. Roots were devided to several parts such a fune root, secondary root, stele and cortex of main root. We ditermined and compared with the major constisuents contained in each parts of 1 and 2 year old roots. Among each parts of root, the amount of constituents in the fine root and cortex were much higher than those in stele of main root. Content of decursin and decursinol angelate was higher 6.5% in 2 year old root than 4.8% in 1 year old root and their component ratio showed 10:7.5 in 1 year old root and 10:6.0 in 2 year old root, respectively. But merits as a material of oriental medicine for 1 year old root that cultivated by early short-time growing seedling were generally acknowledged.

• Korean Journal of Food Science and Technology
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• v.36 no.5
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• pp.847-850
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• 2004

Cooking basic information for indexing of fresh ginseng roots (Panax ginseng C. A. Meyer) was determined. Ginsenoside contents of various age fresh ginseng roots cultivated in Northeast Asia were quantitatively analyzed by HPLC. Average contents of total saponin and each ginsenoside of 4-year-old fresh ginseng root cultivated in Korea were higher than those cultivated fur longer periods (5- and 6-year-old). One-way analysis variance showed average contents of total saponin and each ginsenoside of 4-, 5-, and 6-year-old fresh ginseng roots were not statistically significant. Four-year-old fresh ginseng root cultivated at Geumsan, Korea contained the highest ginsenoside content among samples studied.

• Journal of Ginseng Research
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• v.14 no.1
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• pp.6-9
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• 1990

Specific activities of ADC and ODC from 2-4 year old ones were higher than that from seedlings whereas those activities were not changed significantly from 2 to 4 years. Generally, activity of ADC was predorminant compared to that of ODC. Free arginine content in roots was much higher than that of leaves. And arginase specific activity from roots was higher than that of leaves. Cumulative results suggest that putrscine formation from ornithine in roots may be more effective than leaves and contribute to putrescine biosynthesis to some extract.

• The Plant Pathology Journal
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• v.17 no.5
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• pp.295-299
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• 2001

The relative density of phytoplasmas in witches'broom (WB)-infected jujube trees was investigated using compatitive polymerase chain reaction (PCR). During dormant and defoliating seasons, the densities of phytoplasmas were about the same in roots and twigs. In early growing season, the density showed the highest rates in roots, then in twigs and in petioles. however, the density was highest in petioles and the lowest in roots during actively growing season. Throughout the year, root samples did not show any serious fluctuation compared with that of t2wigs and petioles. Density was lowest during actively growing season in root samples. In contrast, petiole sample densities varied to a great extent depending on the season, very high during actively growing season, but very low during the early growing season, In twig samples, the densities were very high and almost the same in both defoliating and dormant seasons. Among the parts of the trees, phytoplsma density was the most stable in root samples throughout the year. The highest densities of phytoplasmas were about the same in all tree parts. These results suggest that the phytoplasmas may overwinter not only in roots but also in twigs, and that multiplication rate of phytoplsma becomes very high right after the early growing season.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.88-94
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• 2005

Plant tissue culture studies have been done for the preservation of medicinal plant resources and efficient production of pharmaceutically important secondary metabolites. Micropropagation methods for Cephaelis ipecacuanha have been established and these methods enabled much more efficient propagation of the plants than the conventional methods using seedling or layering. The C. ipecacuanha plants derived from tissue culture grew uniformly in the field and they showed higher alkaloid contents compared to the plants grown from seedlings. Hairy root cultures of C. ipecacuanha and Panax ginseng have been established by infection with Agrobacterium rhizogenes, and the production of important pharmaceuticals by these cultures have been successfully demonstrated. In the case of C. ipecacuanha, the highest alkaloid yields from the hairy roots cultured for 8 weeks were 2.75-fold cephaeline (5.5 mg) and one third emetine (0.7 mg) compared with those from the roots of one-year old plant propagated through shoot-tip culture and cultivated in a greenhouse (2.0 mg cephaeline and 2.0 mg emetine). In the case of P. ginseng, ginsenoside contents in the hairy roots optimally cultured for 4 weeks were much higher than those in the roots of 4-year old field-grown plant. Thus our medicinal plant tissue cultures demonstrate desirable properties. However, they are always exposed to danger of microbial contamination or unexpected trouble of culture facilities. Cryopreservation of plant tissue cultures is a reliable method for long-term preservation. Cryopreservation studies on these cultures are also presented.

• Journal of Plant Biotechnology
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• v.5 no.3
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• pp.187-191
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• 2003

This paper reported the establishment of mass production system of adventitious roots of Eleutherococcus sessiliflorus through the shake flask and bio-reactor culture. Induction of adventitious roots was started from the explants of germinated somatic embryos on half-strength Murashing and Skoog (MS) solid medium. The frequency of adventitious root formation was better in the explants comprising the basal hypocotyl parts than root explants alone. Among the different auxins tested (NAA, IBA and IAA), frequency of adventitious root induction was highest on medium with 0.5 mg/L NAA, and produced $16.3\pm1.9$ roots per explant. In shake-flask culture, deletion of $NH_4NO_3$ of MS medium was effective for induction of adventitious root compared with both full and half-strength MS media. Fresh weight increase of induced adventitious roots was performed well in medium with 0.5 mg/L IBA. When adventitious roots produced in shake-flask culture were transferred to 10-liter bioreactor, 5.5 times of fresh weight increase was gained after one month of culture. HPLC analysis revealed that the amount of eleutheroside E and E1 was higher in in vitro cultured adventitious roots than the 3 year-old field cultivated root barks of Eleutherococcus sessiliflorus. The content of eltutheroside B was much lower in adventitious roots than that of field cultivated one.