• Title/Summary/Keyword: rooting media

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Rooting and Acclimatization of Shoots Harvested from Bioreactor Culture in Rehmania glutinosa (생체반응기에서 수확한 지황 신초의 발근과 순화)

  • Koh, Eun-Jung;Chae, Young-Am
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.3
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    • pp.186-188
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    • 2002
  • This experiment was carried out to know the effect of media and agar concentrations, aeration and growth regulators on rooting and acclimatization of the shoots harvested from bioreactor culture in Rehmannia glutinosa. Half MS media with 1.2% agar improved rooting and acclimatization of shoots. Shoots were effectively acclimatized and rooted well in case of aeration by using membrane filtered vessels. Shoots acclimatized in vessel with membrane Inter were healthier and had higher ex vitro survival rate than those without membrane Inter on plug tray. Addition of paclobutrazol 0.3-0.4 mg/L, to acclimatization media enhanced shoots growth and root development.

Effect of Rooting Media on Rooting and Root Growth of Rose Cuttings (장미의 삽목발근에 미치는 삽목용토의 영향)

  • Choi, Byeong-Jin;Sang, Chae-Kyu;Choi, Eun-Joo;Noh, Seol-A
    • Horticultural Science & Technology
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    • v.18 no.6
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    • pp.819-822
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    • 2000
  • This study was conducted to determine the optimum rooting media composition for the production of high-quality rose cuttings. The percentage of rooting was highest in peatmoss (P) and coarse vermiculite (CV) mixture (1:2, v/v). In 'Little Mable' and 'Suplesse', the rate was above 95%, and 'Red Velvet', 'Noblesse', 'Rote Rose', and 'Sweetness' showed 100% rooting rate. 'Rote Rose' showed lowest rooting rate, but in the composition, the rate was 100%. In the composition, root growth was accelerated. Root number, root weight, and root diameter increased in P:CV (1:2) mixture. However, root length increased in peatmoss and perlite mixture (1:2). This results might be caused by the water holding capacity and porosity of the rooting media composition.

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The Promotive Effect of NAA, IBA and Ethychlozate on Rooting Cuttings of Certain Ornamental Plants and Some Physiological Studies. (관상식물 삽목발근에 있어서 NAA, IBA 및 Ethychlozate의 발근촉진효과와 그 생리학적연구)

  • Jeong, Hae-Jun;Gwak, Byeong-Hwa
    • The Journal of Natural Sciences
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    • v.1
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    • pp.115-198
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    • 1987
  • The present studies were undertaken to elucidate the influence of auxins, auxin-like substance-ethychlozate ("Figaron"),and pH and sort of rooting media on rooted propagation of certainornamental woody plant cuttings, and to see possible changes in internal compositions characterizing after root-promoting treatment as the cutting stage proceeded. The experimental check-up srevealed and summarized as seen in the following;I. Effect of three different auxin treatments on rooting cuttings: 1) Promotive influence of auxin varied according to different concentration levels, hours of dipping treatment of the auxins, and kind of plants. The greatest effect was obtained for Forsythia ksreana with NAA and IBA, for Ligustrurn obtusifolium var. variegatum with NAA and ethychlozate, for Hydrangea macrophylla, Magnolia kobus, and Magnolia liliflora with NAA, lBA and ethychlozate also. The most effective level of the promotive agents was found 200mg/l for NAA, 1000mg/l for IBA, and 200mg/l for ethychlozate. For Weigela florida and Gardenia jasminoides, range of the most effective level was shown relatively wide spread. 2) NAA was more effective at its optimal level of the rooting agent than ethychiozate for Weigela florida, Viburnum awabuki, Forsythia koreana, Acer palmatum 'Nomura', Bouga invillea glabra, Elaeagnus umbellata, Prunus tomentosa, Ligustrum obtusifolium, Pyracantha coccinea, Cestrum noctu rnum, Hydrangea macrophylla, Codiaeum variegatum, Rhododen dron lateritium, and Ilex crenata var. macrophylla, and yet ethychlozate was found either as equally as effective or more so than NAA for Zebrina pendula, Hibiscus syriacus, Fatshedera lizei, Schefflera arboricola, Campsis grandiflo ra, Ixora chinensis, Euonymus japonica, and Magnolia liliflora. On the contrary, no the auxin effect was noted with Lagerstroemia indica, Trachelospermum asiaticum, and Syringa vulgaris. This probably indicates that these species are genetically different for the auxin response.II. Effect of different pH and sorts of cutting media on rooting cuttings: 1) Bougainvillea showed best in rooting for the number and dry weight at pH 6.5, more with ethychlozate than NAA, while Ligustrum did at pH 5.0 more with NAA than ethychlozate. pH 4.0 medium resulted in the best rooting for Rhododendron with NAA, more than ethychlozate. 2) Use of cutting medium with peat: perlite: vermiculite = 1:1:1 showed to give the greatest rooting percent and dry weight, apart from considering the number of roots. This apparently meant the fact that cutting medium has more to do with root growth than root differentiation. Rhododendron yet showed results with cutting media that use of peat: perlite = 2:1 mixed is more effective on rooting than using peat alone.III. Effect of auxinic treatments on rooting cuttings and change in some cutting compositions: 1) Under the climatic conditions of July having temperature $26.3\pm$$2.4^{\circ}C$for cutting bed, new roots of Magnolia started to show up generally 20 days after the cutting was made, whereas Cestrum did much earlier than that, namely 14 days after. 2) Although total carbohydrate content of Magnolia cuttings showed no marked change without auxin treatment, it did so with the treatment, especially 30 days after the start of cutting. Cestrum cuttings demonstrated a gradual in crease in total carbohydrate content as rooting took place, and the content became reduced more with auxin than with out, just about when rooting proceeded to 14 days after the start of cutting. 3) Magnolia generally showed an increase in total nitrogen content as rooting proceeded more, and Cestrum showed a decrease in total nitrogen of cuttings. The auxin treatment exhibited no pertinent relation with change in plant nitro gen when rooting is promoted with auxin treatment. 4) An abrupt drop of total sugar and reducing sugar was noticed as Magnolia rooting started, and this reduction was parti cularly outstanding with auxin treatment. Starch content also was decreased in the later stage of cutting with auxin treatment, and was rather increased without auxin. Although sugar content soon increased as cutting started with auxin treatment in the case of Cestrum, it became reduced after rooting took place. 5) Total phenol content increased with rooting, and this was especially true when rooting started. This increase was reversed somehow regardless of auxin treatment. A decrease in phenol of Magnolia was found more striking with auxin than without in the later stage of the cutting period. 6)Avena coleoptile test for auxin-like substances presented the physiologically active factor is more in easy-to-root Magnolia liliflora than hard-to-root Magnolia kobus, and the activity of auxin-like substances was much increased with auxin treatment. The increase in the growth promoting substances was markedly pronounced when rooting just started. The active growth substances decreased in the later stage of cutting, and certain inhibitory substances started appearing. Cestrum also showed physiologically similar growth promoting substances accompanying auxin-like active substances if auxin is treated, and some strong inhibitory substances seemed to appear in the later stage of cutting. 7) Mung-bean-rooting test indicated biologically that endogenous growth substances in Magnolia all promoted mung-bean rooting, and activity of the growth substances apparently stimulated mung-bean rooting with auxin more than without. Here auxin treatment seemed to give a rise to an increased activity of endogenous growth substances in cuttings. This activity was found much greater with either NAA or IBA than ethychlozate, and showed its peak of the activity when rooting first started taking place. Certain inhibitory substances for Avena coleoptile growth strongly promoted mung-bean rooting, and it was also much like in the case of Cestrum.

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Effects of Plant Growth Regulators on in vitro Propagation of Echinosophora koreensis Nakai

  • Yi, Jae-Seon;Lee, Hyunseok;An, Chanhoon
    • Journal of Forest and Environmental Science
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    • v.29 no.4
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    • pp.275-281
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    • 2013
  • To establish in vitro nodal culture conditions of Echinosophora koreensis Nakai, one of rare and endangered species famous for beautiful flowers in the Korean Peninsula, the influence of plant growth regulators (PGRs) on shooting and rooting from in vitro shoots was investigated. In shoot multiplication, addition of 6-benzylaminopurine (BA) to the half-strength Driver and Kuniyuki's media in the range of 2.22 to 8.88 ${\mu}M $induced 2.5 to 2.7 shoots per axillary bud; and addition of 2.27 ${\mu}M $ thidiazuron (TDZ) produced 3.2 shoots, during 4 weeks of culture, while zeatin and isopentenyl adenine (2ip) were not effective on shoot multiplication as observed from several combination treatments of BA with other PGRs. Shoots established were smaller than 2 cm in length, in most of the treatments. while in BA 8.88 ${\mu}M $ treatment more than 30% of shoots were longer than 2 cm and shorter than 4 cm. In rooting, naphthalene acetic acid (NAA) from 5.37 to 21.48 ${\mu}M $ showed the rooting rate from 40.0 to 62.5%. Indole butyric acid (IBA) addition had little effect on rooting (<10%), although some roots in IBA-containing media were longer than those in NAA. Micropropagation from axillary buds of nodular explants was applicable and promising to multiplication and conservation of Echinosophora koreensis Nakai.

In vitro Root Induction from Shoot Explants of Pear (Pyrus spp.)

  • Jae-Young Song;Jinjoo Bae;Woohyung Lee;Jung-Ro Lee;Mun-Sup Yoon
    • Korean Journal of Plant Resources
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    • v.35 no.6
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    • pp.770-777
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    • 2022
  • The main objective of this study was to identify the most appropriate condition for root formation of in vitro micropropagated pear (Pyrus spp.) plants. In vitro propagation was induced on Murashige and Skoog (MS) medium with 2.0 mg/L of N6-benzyladenine (BA) and 0.2 mg/L of Indole-3-butyric acid (IBA) medium. The short pre-treatment of explants with a high concentration (1 mg/L) of NAA and IBA (R0 medium) in dark for three days, followed by transfer to five different media (R1 to R5) resulted in good rooting responses in the pear 'Oharabani (P. pyrifolia × P. communis)' genotype. For the rooting experiments, the highest rooting percentage (83.3 ± 8.3%), average root length (3.6 ± 1.9 mm), total root number (31 ± 4.0), and average root number per plant (2.6 ± 2.1) were obtained on half strength (1/2) of MS medium supplemented with 30 g/L sucrose without hormones and activated charcoal (AC) (R1 medium). The highest rooting percentage was obtained at 83.3% from explants on R1 and R3 media. The rooting procedure described in this study resulted in good root formation and significantly shorting the root induction time to within 14 days of culture. Further studies are underway to test the suitability of the protocol developed in this study for other pear genotypes.

Effect of cutting type, growth regulators and propagation media on rooting and root growth of on Rosa davurica $P_{ALL}$ (생열귀나무 삽목시 발근과 뿌리생장에 미치는 삽수종류, 생장조절물질 및 상토의 효과)

  • 이화영;임정대;김일섭;정일민;유창연
    • Korean Journal of Plant Resources
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    • v.13 no.2
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    • pp.140-146
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    • 2000
  • This study was conducted to determine the effect of cutting type, growth regulators and propagation media on the rooting and root growth of Roea davurica $P_{ALL}$. Three type of cutting, hardwood, half-softwood, softwood cutting and root cutting of Rosa davurica $P_{ALL}$, were used to study the rooting ability. There was no rooting in hardwood cutting while root cutting was appeared 100% of callus formation and rooting. The optimum conditions of softwood cutting for rooting were IAA 100ppm and rooton-F at vermiculite+perlite. The rate of rooting in treatment of rooton-F ranged from 10 to 60%, but such a good effect was not appeared in other growth regulators, IAA, NAA and IBA.

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Production and Rooting of Shoots in Bioreactor Culture of Scrophularia buergeriana Miquel (생물반응기에서 현삼의 신초 형성과 발근)

  • Hahn, Suk-Hoon;Chae, Young-Am
    • Korean Journal of Medicinal Crop Science
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    • v.8 no.2
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    • pp.117-122
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    • 2000
  • This study was carried out to know the factors affecting on shoot formation and rooting for stable and routine production of plantlets in bioreactor culture of Scrophularia buergeriana. Multiple shoots were formed effectively when explants were transplanted on the MS media with decreased concentration of $NH_4NO_3$ as 413mg/ l . Three hundred stem explants (0.8-1.0cm) was appeared as proper inoculation size in bioreactor culture. IBA (0.05mg/L) was more effective for rooting of the shoots in liquid as well as solid media. Six weeks long culture of explants in bioreactor gave better shoot shape for rooting on solid half-strength MS media.

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Micropropagation of Mature Betula davurica by Bud Cultures (물박달나무 (Betula davurica) 성숙목의 아배양에 의한 기내번식)

  • 문지연;문흥규
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.4
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    • pp.271-274
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    • 1999
  • This study was undertaken to develop an efficient propagation technique for mature Betula davurica. Using aseptic materials taken from in vitro culture, the effects of media and plant growth regulators on shoot proliferation and rooting were investigated. DKW medium turned out to be the best in shoot proliferation among the media tested. Whereas axillary buds were better culture material than apical buds in proliferation of shoots, apical buds were slightly better than axillary buds on shoot elongation. Neither 1 /2 MS nor WPM medium seemed to be suitable for shoot multiplication or elongation. When the explants were cultured on 1/2 MS medium, shoot elongation was retarded by forming big callus at the base. In the case of WPM, shoots could be formed normally, but they exhibited slow growing. NAA was so effective on in vitro rooting that more than 80% rooting could be achieved on half-strength DKW medium supplemented with 1.0 mg/L NAA after 4 weeks in cultures. Ex vitro rooting using elongated shoot was also applicable to rooting and acclimatization. Rooted plantlets were successfully acclimatized in an artificial soil mixture and grew normally. The results demonstrate that efficient mass propagation of mature B. davurica can be done through tissue culture.

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In vitro Propagation and Ex vitro Rooting of Tectona grandis (L.f ), APNBV-1 Clone

  • Ramesh, Kommalapati;Chandra, Mouli Kalla;Vijaya, Tartte
    • Journal of Forest and Environmental Science
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    • v.25 no.2
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    • pp.119-126
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    • 2009
  • An efficient in vitro plant regeneration system was developed through shoot proliferation from axillary buds of Tectona grandis (L.f), APNBV-1 (Andhra Pradesh North Badrachalam Venkatapuram-1) clone. Multiple shoots of high quality were produced in vitro from axillary bud explants. An average of 4.39 shoots/explant were obtained on Murashige and Skoog's (MS) medium supplemented with plant growth regulators (PGRs) benzyl amino purine (BA), kinetin (KN), indole acetic acid (IAA), gibberillic acid ($GA_3$), growth adjuvants casein hydrolysate (CH), adenine sulphate (Ads) and antioxidants ascorbic acid, polyvinyl pyrrollidine (PVP). Eighty five percent of rooting was observed in ex vitro rooting media containing IBA and vermiculite. In ex vitro rooting, single shoots with 2 to 3 nodes were subjected to IBA of different concentrations at different periods of time intervals. Direct rooting in vermiculite at 500 ppm concentration of IBA resulted in 4.3 number of roots with 2 cm length. Minimum response of rooting and length of roots were recorded at 100 ppm concentration of IBA. Planlets were transferred to plastic bags for short acclimatization stage in green house where they survived at 95%.

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Effect of Shading Degree and Rooting Media on Growth of Cuttings in Caragana sinica (Buc'hoz) Rehder and Sedum middendorffianum Maxim (차광 정도와 삽목용토가 골담초와 애기기린초의 삽수 생육에 미치는 영향)

  • Kim, Hyun Jin;Kim, Yoon Jin
    • Korean Journal of Medicinal Crop Science
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    • v.23 no.4
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    • pp.271-276
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    • 2015
  • This study was conducted to investigate the effect of shading degree and rooting media on the growth of Caragana sinica and Sedum middendorffianum after cutting. In C. sinica, the highest rooting rate was obtained in cuttings planted in horticultural soil (Sunshine Mix #1) and peat moss mixture (peat moss : perlite = 1 : 1, v/v) under one layer of 35% shading and in cuttings planted in kanumatsuchi soil mixture (kanumatsuchi soil : decomposition of granite = 1 : 1, v/v) under non-shading. Whereas, regardless of shading degree, most cuttings of S. middendorffianum rooted in both horticultural soil and peat moss mixture. Cuttings of C. sinica showed the highest root length, 10.4cm in kanumatsuchi soil mixture under one layer of 35% shading but the highest fresh and dry weight of roots in kanumatsuchi soil mixture under non-shading. In S. middendorffianum, the highest root length, fresh and dry weight of root were obtained in cuttings planted in horticultural soil under non-shading. With these results, we recommended that cuttings of C. sinica should be propagated in kanumatsuchi soil mixture under non-shading and cuttings of S. middendorffianum in horticultural soil under non-shading.