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Rooting and Acclimatization of Shoots Harvested from Bioreactor Culture in Rehmania glutinosa  

Koh, Eun-Jung (School of Plant Science, Seoul National University)
Chae, Young-Am (School of Plant Science, Seoul National University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.47, no.3, 2002 , pp. 186-188 More about this Journal
Abstract
This experiment was carried out to know the effect of media and agar concentrations, aeration and growth regulators on rooting and acclimatization of the shoots harvested from bioreactor culture in Rehmannia glutinosa. Half MS media with 1.2% agar improved rooting and acclimatization of shoots. Shoots were effectively acclimatized and rooted well in case of aeration by using membrane filtered vessels. Shoots acclimatized in vessel with membrane Inter were healthier and had higher ex vitro survival rate than those without membrane Inter on plug tray. Addition of paclobutrazol 0.3-0.4 mg/L, to acclimatization media enhanced shoots growth and root development.
Keywords
Rehmannia glutinosa; Bioreactor; Shoot; Acclimatization; Rooting; Media; Aeration; Plant Growth Regulators;
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