• The Journal of Natural Sciences
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• v.8 no.2
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• pp.153-158
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• 1996

The Number of the disputes originated from vegetable seeds in 1968-1970 under 'Seed and Seedling Law for Agricultural Product)' was 177.: Poor germination was the major one (26% of total disputes), followed by inferior seeds(24.3%), imported seeds(23.7%), and off-type plant(15.6%).During 1976-1995, under 'Administrative Law for Seed and Seedling' the number of disputes originated from vegetable seeds was 175.: 53 in radish, 19 in Chinese cabbage, and 17 in pepper. The type of dispute originated from radish seeds was premature bolting(26%), differences in sowing data(21%), poor purity(19%), micronutrient deficiency(9%), root cracking and branching(9%), poor germination(4%), and damage by disease(2%). In Chinese cabbage, micronutrient deficiency was the major one(27%), followed by poor germination(21%), incomplete head formation(16%), damage by disease(16%), poor purity(10%), and bolting(10%). In pepper, poor fruit setting was the major one(6%), followed by malformed fruits(5%), damage by disease(3%), purity(2%), and poor germination(1%).

• Microbiology and Biotechnology Letters
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• v.20 no.3
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• pp.263-270
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• 1992

For the selection of powerful antagonistic bacterium for biological control of soil borne Eminia carotovora subsp. carotovora causing rot of vegetable, excellent strains (S4, S14, 565) were selected from 1,196 strains of bacteria which were isolated from rhizosphere in vegetable root rot-suppresive soil. Strains were identified to be Pseudomonas species with Api 20NE kit. Antagonistic substance was produced in 523 synthetic broth medium at pH 7~8 and $30^{\circ}C$ during 3 days culture. The substance was stable in the pH range of 6 to 9. When the basal medium was supplemented with mannitol and sorbitol as carbon source and calcium chloride as metal salt, the production of the inhibitory substance was increased. The inhibitory acitivity was increased by the addition of fertilizer in soil. The isolated strains were resistant to the agricultural chemical such as benomyl and fosethyl-Al-folpet, and the antibiotics such as penicillin and lincomycin. We had found that Pseudomonas sp. S14 strain had a single plasmid. After treated with acridin orange for curing, we confirmed the existence of antagonistic gene in the chromosomal DNA.

• Journal of Microbiology and Biotechnology
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• v.32 no.11
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• pp.1435-1446
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• 2022

Zinc-solubilizing bacteria can convert the insoluble form of zinc into soluble forms available to plants. This study was conducted to isolate and screen zinc-solubilizing actinobacteria from rhizosphere soils and to assess their effect on vegetable soybean growth. In total, 200 actinobacteria strains belonging to 10 genera were isolated from rhizosphere soil samples. Among these isolates, four showed zinc solubilization with solubilizing index values ranging from 3.11 to 3.78 on Bunt and Rovira agar supplemented with 0.1% zinc oxide. For the quantitative assay, in broth culture, strains CME34 and EX51 solubilized maximum available zinc contents of 529.71 and 243.58 ㎍/ml. Furthermore, indole-3-acetic acid (IAA) and ammonia were produced by these two strains, the strain CME34 produced the highest amount of IAA 4.62 ㎍/ml and the strain EX51 produced the highest amount of ammonia 361.04 ㎍/ml. In addition, the phosphate-solubilizing abilities in Pikovskaya's medium of CME34 and EX51 were 64.67 and 115.67 ㎍/ml. Based on morphological and biochemical characterization and 16S rDNA sequencing, the strains CME34 and EX51 were closely related to the genus Streptomyces. In a greenhouse experiment, single-strain inoculation of Streptomyces sp. CME34 or EX51 significantly increased the shoot length, root length, plant dry weight, number of pods per plant and number of seeds per plant of vegetable soybean plants compared to the uninoculated control. These findings facilitated the conclusion that the two Streptomyces strains have potential as zinc solubilizers and can be suggested as bioinoculants to promote the growth and yield of soybean.

• Korean Journal of Agricultural Science
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• v.37 no.2
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• pp.191-197
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• 2010

The objective of this research was to determine the influence of the physical and chemical properties of root substrates used during the production of 'Maehyang' strawberry propaguleson the growth of the mother plants and the rate of daughter plant formation. Plants were cultured in plastic bags containing six different formulations of root substrates composed of: a) 50% coir dust and 50% perlite (5:5 by volume, A), b) 60% coir dust and 40% perlite (6:4, B), c) 70% coir dust and 30% perlite (7:3, C), d) 70% coir dust and 30% coconut chip (7:3 D), e) 60% coir dust and 40% coconut chip (60:40, E), or f) 50% sphagnum peat and 50% vermiculite (50:50, F). All media formulations contained a moderate level of base fertilizers. Physical and chemical properties of each formulation were determined before plant establishment and after 120 days of stock plant culture and runner production. Total porosity (TP) and container capacity (CC) of all substrate formulations were higher than 85% and 55%, respectively, allowing a suitable range of air and water holding characteristics. Formulation F provided the highest TP and CC values among the all substrate modifications evaluated. Substrate formulations A, B, C and F had higher electrical conductivity (EC) and $NO_3{^-}$-N concentrations than formulations D and E, when determined before and after plant culture. Formulations A, B, C, and F, having higher EC readings, also performed better as root substrates thanthe formulations D and E in increasing fresh and dry weights of the runners as well as the production of daughter plants per plant. The 'Maehyang' strawberry plants grown in the formulation F had the highest tissue N content, followed by those grown in substrate B, A, C, or D for 120 days after transplanting. Formulation F also facilitated accumulation of higher tissue phosphorus (P) and copper (Cu) contents compared to other treatments. Results of this experiment suggest that the chemical properties, rather than physical properties, of root substrates had a major influence on the growth of mother plants and the occurrence of healthy daughter plants during the bag-culture phase of propagation.

• Microbiology and Biotechnology Letters
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• v.39 no.2
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• pp.146-152
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• 2011

In the course of a study in relation to the production of taste, and functional enhancements in root crop chips, which were prepared by soaking dried yam slices in fruit juices, we investigated the physiological characteristics and biological activities of 8 different commercially available juices including; apple, omija (fruit of Maximowiczia typica), grape, wild grape, orange, tomato, red ginseng and black garlic juice. The average water contents, pH, brix and acidity of the juices used were $85.59{\pm}5.80%$, $3.90{\pm}0.64$, $12.19{\pm}4.70%$, and $0.49{\pm}0.19%$, respectively. The polyphenol content of black garlic and grape juice were 1.50 and 1.21 mg/ml, respectively, and those were higher than the average content (0.57 mg/mL) of the juices. Evaluation of anticoagulation activity showed that only omija juice has a strong thrombin inhibition, which is comparable to that of aspirin (1.5 mg/mL). Omija, grape and orange juice all exhibited antibacterial activity, but no antifungal activity. The 8 different juices, and in particular grape and black garlic juice, showed strong antioxidant activity in DPPH and ABTS radical scavenging activity assays, with wild grape juice demonstrating potent nitrite scavenging activity. These results suggest that omija, grape and black garlic juice can be used as soaking solutions to produce taste, and other functional enhancements, for root crop chips.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.4
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• pp.317-322
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• 2009

Healthy seedling generation is the major concern in overcoming adverse effects of biotic and abiotic stresses during tender stage of development in vegetables and horticultural crops. Because of this, priority is given to research leading to the generation of healthy seedlings in crops subjected to transplanting and bedding. In this study, growth pouch experiments were conducted to determine the effect of inoculation of six different strains of Methylobacterium sp. namely, M. oryzae CBMB20, M. phyllosphaerae CBMB27, M. suomiense CBMB120, and Methylobacterium strains CBMB12, CBMB15 and CBMB17 on the seedling development of the vegetable crops cabbage, Chinese cabbage and cucumber; and horticultural crops tomato and red pepper. Crops treated with the test strains generally showed higher seedling dry matter accumulation compared to the control. Significantly higher accumulation was exhibited by CBMB12, CBMB17, and CBMB20 in cabbage, as well as for CBMB27 and CBMB120 on tomato and Chinese cabbage, respectively. Furthermore, all the strains promoted root elongation in cucumber and tomato seedlings while in Chinese cabbage and red pepper, root elongation was observed with CBMB120 and CBMB12 inoculation, respectively. Large scale nursery study is needed to develop a thorough protocol for healthy seedling development with the use of these strains.

• Korean Journal of Plant Resources
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• v.9 no.2
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• pp.143-149
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• 1996

The study was carried out to find the optimum planting density classified by the different using types in Anthriscus sylvestylis Hoffm. In the different using types, plant height of vegetable type in $30\times20cm$ was 35.2cm, but plant heights of other types were much lower than that of vegetable type. Fresh leaf yield was highest in the vegetable type at the $30\times20cm$ with 3,300kg, in the medicinal type at the $60\times30cm$ with 2,800kg, and in the combination type at the $40\times20cm$, In comparison of underground parts of dry root weight, the highest yield was 3,320kg at $30\times20cm$ of the vegetable type, 2,170kg at $60\times20cm$ of medicinal type, and 2,210kg per ha. at $45\times20cm$ of combination type, respectively. It was showed that the optimum planting densities were $30\times20cm$ in vegetable type, $60\times30cm$ in medicinal type, and $45\times20cm$ in combination type.

• Journal of Bio-Environment Control
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• v.22 no.4
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• pp.421-426
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• 2013

This study was conducted to investigate the effects of timing of nutrient starvation during transplant production on growth of runner plants and yield of strawberry 'Seolhyang' (Fragaria ${\times}$ ananassa). Nutrient solution supply at the level of EC (electrical conductivity) 0.8 $dS{\cdot}m^{-1}$ was terminated at interval about 10 days between July 25 and September 5. As a result, the growth of above-ground part was inhibited while root growth increased when the nutrient starvation treatment had been brought forward to July 25. It also reduced the T/R ratio significantly and chlorophyll content was tended to be lower than the other treatment. In addition, it significantly promoted the budding, flowering and harvest of first flower cluster. On the other hand, the period of harvest was delayed more than two weeks when the nutrients were continuously supplied after the middle of August. An accumulated marketable fruit yield per plant until the end of January and February was 169 and 266g, respectively in the treatment of nutrient starvation on July 25, which was 71 and 12% increase, respectively, as compared with those in the treatment of September 5. Therefore, the appropriate nutrient starvation in the late season of strawberry nursery period could be expected the increase in yield and income during the winter season by promoting the flower bud differentiation as reducing the endogenous nitrate level of the plantlet.

• Journal of Bio-Environment Control
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• v.26 no.4
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• pp.368-377
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• 2017

This study investigated the possibility of application of the recently introduced cylindrical paper pot seedlings in hot pepper (Capsicum annuum L.) cultivation. The seedling growth, initial rooting after planting and accumulated fruit yield were investigated with the treatments of tray type (paper pot and plug) as a main factor, tray cell number (40 cell and 50 cell) as a sub-factor, and fertigation method (continuous fertigation and fertigation after 35 days sowing) as a sub-sub factor, respectively. The growth of pepper seedlings was significantly affected by tray type and fertigation method showing the highest value at 50 cell plug tray with continuous fertigation, and the effect of fertigation was greater than that of trays. 'Cheongyang', 'Daekwonseoneon' and 'Longgreenmat' cultivar showed all the same pattern in seedling growth. These three-cultivar seedlings were planted in plastic house and in open field in Jeonju area, respectively, and another 'Daekwonseoneon' seedlings raised Yeongyang local area was also planted at the same area. There was no difference in the rooting of 'Cheongyang' pepper at 2 weeks after planting in plastic house. The accumulated fruit weight was not significantly different between paper pot seedlings and plug seedlings in three cultivar grown in plastic house. However, that of 'Cheongyang' pepper showed higher at paper pot seedlings than plug seedlings and the other two cultivar were higher at plug seedlings in open field. 'Daekwonseoneon' pepper yield grown in open field in 'Yeongyang' area was not significant between paper pot seedlings and plug seedlings. In conclusion, the pattern of seedlings growth grown in the cylindrical paper pot was the same as those of the conventional plug seedlings and also fruit yield was similar between paper pot seedlings and plug seedlings even though minute difference among cultivars. These results suggest that pepper seedlings grown in paper pot should be highly applicable to pepper cultivation.