• 제목/요약/키워드: root media

검색결과 503건 처리시간 0.023초

인삼(人蔘)의 조직배양(組織培養) (Ginseng Tissue Culture)

  • 이재두
    • 생약학회지
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    • 제3권2호
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    • pp.65-72
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    • 1972
  • Tissues of $Panax\;Schinseng\;N_{EES}\;root$ were cultured on the synthetic agar media to investigate the nutrient efficiency on the callus induction and organ formation. The differentiation pattern of the callus mass and the structure of the induced organ (root) were observed internally. On White's medium, callus formation needed the supplement of 2,4-D (5mg/l) and kinetin (1.0mg/l), and on MS medium the root induction NAA (0.2mg/l) and kinetin (0.1mg/l). In order to investigate the effect of inorganic components on callus formation, the inorganic part of White' medium was substituted with those of Heller, Murashige Skoog, and Earle. As the result culture Earle's was most effective. On the other hand, the roots were induced from the meristem in the deep region of callus mass. Since this meristem is similar to the pericambium of tap root, they are the same on the pattern of morphogenesis.

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Propagation of Three Rubus Species Native to Korea by Stem Cutting

  • Cheong, Eun Ju
    • Journal of Forest and Environmental Science
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    • 제34권2호
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    • pp.173-175
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    • 2018
  • Stem cuttings of three Rubus species native to Korea were treated by two commercial root stimulation formula for propagation. Differences among the species was obvious, toot was induced relatively easily on R. hongnoensis. Fifty percent of stem rooted without any treatment on this species and two stimulators was effective to increase the root induction. On the other hand, two other species, R. schizostylus and R. ribisoideus, had very low percent of rooting. Treatment using root stimulators was effective but still lower than that of R. hongnoensis. Root induction was not influence by the medium that resulted no differences between two media.

Optimization of the Sucrose and Ion Concentrations for Saikosaponin Production in Hairy Root Culture of Bupleurum falcatum

  • Ahn, Jun-Cheul;Chong, Won-Seog;Kim, Young-Soon;Hwang, Baik
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제11권2호
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    • pp.121-126
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    • 2006
  • Saikosaponin productivity was examined in a Bupleurum falcatum L. BFHR2 hairy root culture in response to changes in the sucrose content $(2{\sim}8%)$, nitrogen content $(0{\sim}250mM\;NH_4NO_3)$, phosphate content $(0{\sim}12mM\;NaH_2PO_4)$, and the potassium content $(0{\sim}87.2 mM\; KCl)$ of the culture media. We found that the conditions for maximal saikosaponin production differed from those for optimal root growth. Highest saikosaponin yield was achieved for 8% sucrose, 62mM $NH_4NO_3$ 1.2 mM $NaH_2PO_4$, and 0.5mM KCl.

인삼이 신경 및 근육 세포에 미치는 영향에 대한 연구 (Studies on the Effect of Ginseng Extract on Chick Embryonic Nerve and Muscle Cells)

  • 김영중;김은경
    • 약학회지
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    • 제24권3_4호
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    • pp.143-150
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    • 1980
  • The effect of ginseng saponin on chick embryonic dorsal root ganglia organ culture and brain, spinal cord, muscle dissociation cultures was studied. The fiber outgrowth in explanted chick embryonic dorsal root ganglia was markedly induced by water and alcohol extracts of ginseng, total ginseng saponin, protopanaxadiol and protopanaxatriol glycosides as well as ginsenosides R/sub b1/, R/sub d/, R/sub 0/+R/sub a/+R/sub b1/, and R/sub b2/+R/sub c/+R/sub e/ mixtures. The life span of the cultured chick embryonic dorsal root ganglia and potentiation of nerve cell density were also observed with all of these ginseng saponins. The effect of ginseng saponin on chick embryonic dorsal root ganglia organ culture was more marked in the absence of the chick embryonic extract which was known to contain nerve growth factor-like material in the culture media. However, the ginseng saponin did not influence the cultured central nervous system such as brain and spinal cord cells and cultured skeletal muscle cells with respect to the morphological changes, maturation and life span of these cells.

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개인미디어의 충성도에 영향을 미치는 요인 (The Factors Influencing on the Loyalty of Self-Media)

  • 섭언정;김인재
    • 한국정보시스템학회지:정보시스템연구
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    • 제27권3호
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    • pp.103-121
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    • 2018
  • Purpose As web 2.0 has taken root in the Internet environment, people began to create specific values to produce and disseminate contents on their own. In order to emphasize the personalized media attributes, the concept of self media was derived. In this paradigm shift, self media has emerged that succeeded in commercializing themselves in social media. Taking advantage of the influence of self media will have a tremendous positive impact on management activities such as marketing strategy, brand promotion, customer retention, and profit growth. This study examines the factors affecting the loyalty of self media in Korea and China because the studies in the area of selfmedia is not enough. Design/methodology/approach This study analyzed what factors influenced the loyalty of self-media in terms of Korea-China comparison. The research model was proposed on the basis of previous research papers. Content value, interactivity, ease of access, self-congruity, sympathy, and reputation were used as independent variables. Trust and satisfaction are used as mediating variables, nationality as moderator variables, and loyalty to self-media as a dependent variable. Results As a result, it was found that all independent variables directly influence the loyalty of self-media except interactivity, and interactivity indirectly influence loyalty through trust and satisfaction. There was a moderating effect of nationality between satisfaction and loyalty, but was not a moderating effect between trust and loyalty.

PLANT REGENERATION THROUGH SOMATIC EMBRYOGENESIS IN ROOT-DERIVED CALLUS OF GINSENG

  • Chang Wei-Chin;Hsing Yue-Ie
    • 고려인삼학회:학술대회논문집
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    • 고려인삼학회 1980년도 학술대회지
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    • pp.21-25
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    • 1980
  • Callus culture was initiated from explants of mature root tissues of ginseng (Panax ginseng C. A. Meyer) on MS medium enriched with 2, 4-D. The aging callus produced numerious embryoids in the same medium. Reculture of these embryoids in the media (1/2 MS or B5) supplemented with benzyladenine and gibberellic acid resulted in profuse plantlet regeneration.

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인삼 뿌리썩음 병균, Fusarium solani에 길항적인 Streptomyces species의 동정 (Identification of Streptomyces species antagonistic to Fusarium solani causing Ginseng root rot)

  • 정영윤;정후섭;오승환
    • 미생물학회지
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    • 제20권2호
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    • pp.73-79
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    • 1982
  • Among 131 isolates of Strptomyces obtained from ginseng cultivating soil, the two isolates ST59 and ST129 showing high antagonistic activity to Fusarium solani(Mart.) Appel & We. causing ginseng root rot were identified. The two isolates were identified Streptomyces alboniger Porter, et al. and Strptomyces reseolilacinus Pridham, et al., respectively, based on mrophology, cultural, and physiological characteristics on various culture media. Spore chains of ST59 and ST129 were flexuous(RF) and coiled(S). Spore surfaces of two isolates were all smooth. Aerial mass color of ST59 was white series and ST129 red series.

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상태 표현 방식에 따른 심층 강화 학습 기반 캐릭터 제어기의 학습 성능 비교

  • 손채준;이윤상
    • 한국방송∙미디어공학회:학술대회논문집
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    • 한국방송∙미디어공학회 2021년도 하계학술대회
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    • pp.14-15
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    • 2021
  • 물리 시뮬레이션 기반의 캐릭터 동작 제어 문제를 강화학습을 이용하여 해결해 나가는 연구들이 계속해서 진행되고 있다. 이에 따라 이 문제를 강화학습을 이용하여 풀 때, 영향을 미치는 요소에 대한 연구도 계속해서 진행되고 있다. 우리는 지금까지 이뤄지지 않았던 상태 표현 방식에 따른 강화학습에 미치는 영향을 분석하였다. 첫째로, root attached frame, root aligned frame, projected aligned frame 3 가지 좌표계를 정의하였고, 이에 대해 표현된 상태를 이용하여 강화학습에 미치는 영향을 분석하였다. 둘째로, 동역학적 상태를 나타내는 캐릭터 관절의 위치, 각도에 따라 학습에 어떠한 영향을 미치는지 분석하였다.

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Mass Production of Adventitious Roots of Eleutherococcus sessiliflorus through the Bioreactor Culture

  • Seo Jin-Wook;Shin Cha-Gyun;Choi Yong-Eui
    • Journal of Plant Biotechnology
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    • 제5권3호
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    • pp.187-191
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    • 2003
  • This paper reported the establishment of mass production system of adventitious roots of Eleutherococcus sessiliflorus through the shake flask and bio-reactor culture. Induction of adventitious roots was started from the explants of germinated somatic embryos on half-strength Murashing and Skoog (MS) solid medium. The frequency of adventitious root formation was better in the explants comprising the basal hypocotyl parts than root explants alone. Among the different auxins tested (NAA, IBA and IAA), frequency of adventitious root induction was highest on medium with 0.5 mg/L NAA, and produced $16.3\pm1.9$ roots per explant. In shake-flask culture, deletion of $NH_4NO_3$ of MS medium was effective for induction of adventitious root compared with both full and half-strength MS media. Fresh weight increase of induced adventitious roots was performed well in medium with 0.5 mg/L IBA. When adventitious roots produced in shake-flask culture were transferred to 10-liter bioreactor, 5.5 times of fresh weight increase was gained after one month of culture. HPLC analysis revealed that the amount of eleutheroside E and E1 was higher in in vitro cultured adventitious roots than the 3 year-old field cultivated root barks of Eleutherococcus sessiliflorus. The content of eltutheroside B was much lower in adventitious roots than that of field cultivated one.

Effects of Solar Heating for Control of Pink Root and Other Soil-borne Diseases of Onions

  • Lee, Chan-Jung;Lee, Jong-Tae;Moon, Jin-Seong;Ha, In-Jong;Kim, Hee-Dae;Kim, Woo-Il;Cheon, Mi-Geon
    • The Plant Pathology Journal
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    • 제23권4호
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    • pp.295-299
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    • 2007
  • These experiments were carried out to examine efficacy of soil solarization for control of pink root disease by means of mulching with transparent polyethylene sheets in the hot season. The effects of soil solarization on incidence of pink-root disease caused by Pyrenochaeta terrestris and on onion growth and on populations of soil fungi were investigated. Solarization was dramatically effective in reducing pink root incidence in onion seedling and harvested onion bulb. A 30-day and 40-day solarization treatment significantly improved seedling survival and increased yield of 'Changnyeong-deago' onion while decreasing incidence of pink root. Populations of soil fungi from fields planted to onion were assayed on selective media. Solarization treatment was effective in reducing populations of P. terrestris, Pythium spp., and Rhizoctonia sp. in soil. Increase of yield of onion bulbs was associated with control of soil-borne pathogenic fungi. Soil solarization had beneficial effects on yield, bulb diameter, or incidence of pink root.