Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Optimization of the Sucrose and Ion Concentrations for Saikosaponin Production in Hairy Root Culture of Bupleurum falcatum

  • Ahn, Jun-Cheul (Department of Life Sciences, Seonam University) ;
  • Chong, Won-Seog (Department of Pharmacology, Medical School, Seonam University) ;
  • Kim, Young-Soon (Kumho Life & Environmental Science Laboratory, Chonnam National University) ;
  • Hwang, Baik (Department of Biology, Chonnam National University)
  • Published : 2006.04.30
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Abstract

Saikosaponin productivity was examined in a Bupleurum falcatum L. BFHR2 hairy root culture in response to changes in the sucrose content $(2{\sim}8%)$, nitrogen content $(0{\sim}250mM\;NH_4NO_3)$, phosphate content $(0{\sim}12mM\;NaH_2PO_4)$, and the potassium content $(0{\sim}87.2 mM\; KCl)$ of the culture media. We found that the conditions for maximal saikosaponin production differed from those for optimal root growth. Highest saikosaponin yield was achieved for 8% sucrose, 62mM $NH_4NO_3$ 1.2 mM $NaH_2PO_4$, and 0.5mM KCl.

Keywords

References

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