Browse > Article

Optimization of the Sucrose and Ion Concentrations for Saikosaponin Production in Hairy Root Culture of Bupleurum falcatum  

Ahn, Jun-Cheul (Department of Life Sciences, Seonam University)
Chong, Won-Seog (Department of Pharmacology, Medical School, Seonam University)
Kim, Young-Soon (Kumho Life & Environmental Science Laboratory, Chonnam National University)
Hwang, Baik (Department of Biology, Chonnam National University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.2, 2006 , pp. 121-126 More about this Journal
Abstract
Saikosaponin productivity was examined in a Bupleurum falcatum L. BFHR2 hairy root culture in response to changes in the sucrose content $(2{\sim}8%)$, nitrogen content $(0{\sim}250mM\;NH_4NO_3)$, phosphate content $(0{\sim}12mM\;NaH_2PO_4)$, and the potassium content $(0{\sim}87.2 mM\; KCl)$ of the culture media. We found that the conditions for maximal saikosaponin production differed from those for optimal root growth. Highest saikosaponin yield was achieved for 8% sucrose, 62mM $NH_4NO_3$ 1.2 mM $NaH_2PO_4$, and 0.5mM KCl.
Keywords
saikosaponin; hairy root; sucrose; macro-nutrients; productivity;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
Times Cited By SCOPUS : 5
연도 인용수 순위
1 Yamamoto, M., A. Kumagai, and Y. Yamamura (1975) Structure and actions of saikosaponin isolated from Bupleurum falcatum L. II. Metabolic actions of saikosaponins, especially a plasma cholesterol-lowering action. Arzneimittelforschung 25: 1240-1243
2 Kita, T., T. Hata, E. Itoh, and R. Yoneda (1980) Analgesic and other pharmacological actions of saikosaponin in repeated cold stress (SART stressed) animals. J. Pharmacobiodyn. 3: 269-280   DOI
3 Just, J., C. Recio, R. Giner, M. J. Cuellar, S. Manez, J. L. Rios, A. R. Bilia, and I. Morelli (1996) Preliminary study of the pharmacology and phytochemistry of Bupleurum rotundifolium. Phytother. Res. 10: S69-S70
4 Abe, H., M. Sakaguchi, H. Konishi, T. Tani, and S. Arichi (1978) The effects of saikosaponins on biological membranes. 1. The relationship between the structures of saikosaponins and hemolytic activity. Planta Med. 34: 160-166   DOI
5 Abe, H., S. Odashima, and S. Arichi (1978) The effects of saikosaponins on biological membranes. 2. Changes in electron spin resonance spectra from spin-labelled erythrocyte ghost membranes. Planta Med. 34: 287-290   DOI
6 Ushino, Y. and H. Abe (1991) The effects of saikosaponin on macrophage functions and lymphocyte proliferation. Planta Med. 57: 511-514   DOI   ScienceOn
7 Tanaka, T., E. Sakai, M. Mizuno, T. Kawamura, Y. Hista, K. Okuda, Y. Noro, Z. X. X. Zheng, and D. Fang (1988) Cultivation and saponin contents of Guangxi Bupleurum. Jap. J. Pharmacogn. 42: 236-239
8 Park, Y. J., J. D. Seong, H. Y. Kim, H. S. Suh, and J. W. Shim (1994) Root yield and saikosaponin content in local strains of Bupleurum falcatum L. Kor. J. Crop Sci. 39: 453-457   과학기술학회마을
9 Hosoda, K., M. Noguchi, T. Ikenaga, Y. Hisata, and Y. Noro (1995). Studies on the cultivation of Bupleurum falcatum L. (VI). Variation in lignification index of B. falcutum of different geographical origins. Nat. Med. 49: 11-13
10 Chang, S. M., B. Y. Park, and J. Choi (1990) Effects of the soil fertility and the inorganic nutrients in the root on the contents of saikosaponin a, c in the root of Bupleurum falcatum L. J. Kor. Soc. Soil Sci. & Fert. 23: 49-52
11 Minami, M. and M. Sugino (1995) Effects of mineral fertilizers on growth and saikosaponins contents of Bupleurum falcatum L. (I). Effects of different levels of nitrogen, phosphoric acid, and potassium on growth and saikosaponins contents of one-year-old plant. Nat. Med. 49: 230-239
12 Aoyagi, H., Y. Kobayashi, K. Yamada, M. Yokoyama, K. Kusakari, and H. Tanaka (2001) Efficient production of saikosaponins in Bupleurum falcatum root fragments combined with signal transducers. Appl. Microbiol. Biotechnol. 57: 482-488   DOI
13 Hirasuna, T. J., L. J. Pestchanker, V. Srinivasan, and M. L. Shuler (1996) Taxol production in suspension cultures of Taxus baccata. Plant Cell Tissue Organ Cult. 44: 95-102   DOI
14 Jeong, G. T. and D. H. Park (2005) Enhancement of growth and secondary metabolite biosynthesis: Effect of elicitors derived from plants and insects. Biotechnol. Bioprocess Eng. 10: 73-77   과학기술학회마을   DOI   ScienceOn
15 Jeong, G. T. and D. H. Park (2006) Characteristics of transformed Panax ginseng C.A. Meyer hairy roots: Growth and nutrient profile. Biotechnol. Bioprocess Eng. 11: 43-47   과학기술학회마을   DOI   ScienceOn
16 Uomori, A., S. Seo, and Y. Tomita (1974) Studies on the constituents in tissue cultures of Bupleurum falcatum L. Shoyakugaku Zasshi 28: 152-160
17 El Badaoui, H., P. Morard, and M. Henry (1996) Stimulation of the growth and solamargine production by Solanum paludosum multiple shoot cultures using a new culture media. Plant Cell Tissue Organ Cult. 45: 153-158   DOI
18 Watanabe, T. T., S. Yoshikawa, A. Isoda, H. Takada, A. Ishiguro, H. Namera, K. Kohda, J. Malla, and A. Takano (1998) Studies on the medicinal plant resources of the Himalayas (2). Qualitative and quantitative evaluation of saikosaponins of some Bupleurum roots collected in Nepal. Nat. Med. 52: 421-425
19 Nishiyama, T., N. Akutsu, and Y. Nakayama (1999). Effects of saikosaponin b1 on the synthesis of VII type collagen. Seikagaku 71: 889
20 Ushino, Y. and H. Abe (1991) Effects of saikosaponin d on the functions and morphology of macrophages. Int. J. Immunopharmacol. 13: 493-499   DOI   ScienceOn
21 Lee, K. T., B. J. Kim, J. H. Kim, M. Y. Heo, and H. P. Kim (1997) Biological screening of 100 plant extracts for cosmetic use (I): inhibitory activities of tyrosinase and DOPA auto-oxidation. Int. J. Cosmet. Sci. 19: 291-298   DOI   ScienceOn
22 Kusakari, K., M. Yokoyama, and S. Inomata (2000) Enhanced production of saikosaponin by root culture of Bupleurum falcatum L. using two-step control of sugar concentration. Plant Cell Rep. 19: 1115-1120   DOI
23 Zhong, J. J. and Q. X. Zhu (1995) Effect of initial phosphate concentration on cell growth and ginsenoside saponin production by suspended cultures of Panax notoginseng. Appl. Biochem. Biotechnol. 55: 241-247   DOI
24 Wang, Y. Z. and Y. Y. Zhang (1994) Determination of species of medicinal Bupleurum. Chinese Pharmaceutical J. 29: 16-18
25 Ahn, J. C., Y. W. Paek, C. K. Sung, G. H. Kang, and B. Hwang (1993) Production of saponin content by hairy root culture of Bupleurum falcatum. 1. Comparison of saponin content and pattern in callus, adventitious root, hairy root, and cultivated root. Kor. J. Botany 36: 43-49
26 Mizukami, H., K. Matsunaga, H. Ohashi, A. Amano, T. Maekawa, and K. Fujimoto (1991) Variation in saikosaponin content of Bupleurum falcatum L. of different geographical origins. Jap. J. Pharmacogn. 45: 342-344
27 Yamada, J., K. Hujita, and K. Sakai (2003) Effect of major inorganic nutrients on $\beta$-thujaplicin production in a suspension culture of Cupressus lusitanica cells. J. Wood Sci. 49: 172-175   DOI
28 Park, Y. G., S. J. Km, Y. M. Kang, H. Y. Jung, D. T. Prasad, S. W. Kim, Y. G. Chung, and M. S. Choi (2004) Production of ginkgolides and bilobalide from optimized the Ginkgo biloba cell culture. Biotechnol. Bioprocess Eng. 9: 41-46   DOI   ScienceOn
29 Murashige, T. and F. Skoog (1962) A revised media for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473-497   DOI
30 Paek, Y. W., J. C. Ahn, B. G. Jung, S. U. Kim, and B. Hwang (1993) Betaline production by hairy root cultures of red beet (Beta vulgaris L.). Kor. J. Plant Tissue Cult. 20: 159-165
31 Ahn, J. C, Y. J. Jeong, K. B. Lee, O. T. Kim, and B. Hwang. (1999b) The effects of variations culture media on histological anatomy and saikosaponin content in hairy root culture of Bupleurum falcatum. Kor. J. Medicinal Crop. 7: 251-256   과학기술학회마을
32 Fulcheri, C., P. Morard, and M. Henry (1998) Stimulation of the growth and the triterpenoid saponin accumulation of Saponaria officinalis cell and Gypsophila paniculata root suspension cultures by improvement of the mineral composition of the media. J. Agric. Food Chem. 46: 2055-2061   DOI   ScienceOn
33 Nose, M., S. Amagaya, and Y. Ogihara (1989) Effects of saikosaponin metabolites on the hemolysis of red blood cells and their adsorbability on the cell membrane. Chem. Pharm. Bull. 37: 3306-3310   DOI
34 Srinivasan, V. and D. D. Y. Ryu (1993) Improvement of shikonin productivity in Lithospermum erythrorhizon cell culture by alternating carbon and nitrogen feeding strategy. Biotechnol. Bioeng. 42: 793-799   DOI   ScienceOn
35 Ushino, Y. and H. Abe (1992) Inactivation of measles virus and herpes simplex virus by saikosaponin d. Planta Med. 58: 171-173   DOI   ScienceOn
36 Kim, K. S., Y. A. Chae, and B. H. Lee (2000) Comparison of saikosaponin content in Bupleurum falcatum L. cultivars, 'Jeongsun' and 'Mishima'. Kor. J. Breed. 32: 233-238
37 Shimokawa. Y., I. Okuda, M. Kuwano, and H. Ohashi (1980) Cultivation and breeding of Bupleurum falcatum L. (Ⅵ). Geographical variation of B. falcatum. Jap. J. Pharmacogn. 34: 239-244
38 White, F. F. and E. W. Nester (1980) Relationship of plasmids responsible for hairy root and crown gall tumorigenicity. J. Bacteriol. 144: 710-720
39 Touria, E. J. and C. Douglas (1998) Effect of plant age on nitrogen uptake and distribution by greenhouse plants. J. Plant Nutr. 21: 1055-1066   DOI   ScienceOn
40 Ahn, J. C., E. S. Kim, H. J. Lee, and B. Hwang (1999) Effects of media and IAA and IBA on growth and saikosaponin production with Bupleurum falcatum hairy root culture. Kor. J. of Plant Tissue Cult. 26: 171-175
41 Satake, M. (1991) Production and resources of Bupleurum root (root of sickle hare's ear). Gendai Toyo Igaku 12: 71-78