• Korean Journal of Medicinal Crop Science
• /
• v.28 no.2
• /
• pp.142-151
• /
• 2020

Background: Suppressive soil inhibits soil-borne diseases if pathogens are present, and ginseng does not show injury even if replanted in the same field. Methods and Results: Soil chemical properties and microbial community of soil were investigated in soil suppressive and conducive to ginseng root rot. Root rot disease in 2-year-old ginseng was tested by mixing conducive soil, with suppressive or sterilized suppressive soil. The root rot ratio in suppressive soil was 43.3% compared to 96.7% in conducive soil. Biological factors acted to inhibit the root rot because disease ratio was increased in the sterilized suppressive soil compared to that in non-suppressive soil. The suppressive soil had lower pH, nitrate nitrogen and sodium than the conducive soil. Dominat bacteria and fungi (more than 1.0%) were 3 and 17 species in conducive soil and 7 and 23 species in suppressive soil, respectively. The most predominant fungi were Pseudaleuria sp. HG936843 (28.70%) in conducive soil and Pseudogymnoascus roseus (7.52%) in suppressive soil. Conclusion: Microbial diversity was more abundant in the suppressive soil than in the conducive soil, and the proportion of pathogens (Nectriaceae sp.) causing root rot was significantly lower in the suppressive soil than in the conducive soil.

• Korean Journal Plant Pathology
• /
• v.14 no.6
• /
• pp.594-597
• /
• 1998

The optimal quantity of inoculum was determined to screen resistance of garlic cultivars against Sclerotium cepivorum and 30 cultivars was tested. The growth of the pathogen in detached roots, Disease incidence was increased when the inoculum density was raised form 10 to 100 sclerotia. The optimal inoculum density to differentiate resistance or susceptibility of garlic cultivars was seemed to be 50 sclerotia. The cultivars collected from England, Japan, Nepal and Turkey, and cultivars such as common red, PI1356104 and PI135693 were less than the other cultivars in their disease incidence. The growth of S. cepivorum in detached roots varied from 23 to 33 mm according to garlic cultivars. There was no relationship between the disease incidence and the growth in detached roots. The sclerotial germination was increased significantly when root extract was extract was added. The addition of only distilled water resulted in 13% germination, but the addition of 0.25 g of root extract in 100 ml distilled water resulted in more than 85% germination. There was no difference in the stimulation of sclerotial germination among cultivars which showed different resistance.

• The Plant Pathology Journal
• /
• v.35 no.3
• /
• pp.219-233
• /
• 2019

Soybean cultivars susceptible to Phytophthora root and stem rot are vulnerable to seed rot and damping-off of seedlings and young plants following an infection by Phytophthora sojae. In this study, the disease responses of Japanese soybean cultivars including currently grown main cultivars during the early growth stages were investigated following infections by multiple P. sojae isolates from Japanese fields. The extent of the resistance to 17 P. sojae isolates after inoculations at 14, 21, and 28 days after seeding varied significantly among 18 Japanese and two US soybean cultivars. Moreover, the disease responses of each cultivar differed significantly depending on the P. sojae isolate and the plant age at inoculation. Additionally, the treatment of 'Nattosyo-ryu' seeds with three fungicidal agrochemicals provided significant protection from P. sojae when plants were inoculated at 14-28 days after seeding. These results indicate that none of the Japanese soybean cultivars are completely resistant to all tested P. sojae isolates during the first month after sowing. However, the severity of the disease was limited when plants were inoculated during the later growth stages. Furthermore, the protective effects of the tested agrochemicals were maintained for at least 28 days after the seed treatment. Japanese soybean cultivars susceptible to Phytophthora root and stem rot that are grown under environmental conditions favorable for P. sojae infections require the implementation of certain practices, such as seed treatments with appropriate agrochemicals, to ensure they are protected from P. sojae during the early part of the soybean growing season.

• Korean Journal of Plant Resources
• /
• v.17 no.2
• /
• pp.116-121
• /
• 2004

Panax ginseng discarded and lower than 4th grade is caused by red skin disease showing red color skin in ginseng. This kind of red skin ginseng is found a lot in Panax ginseng rather than Panax quinquefolium, and it is considered that red skin disease might be caused by gene. Therefore, this study was carried out to detect genes resistant to red skin disease using RT-PCR. RNA was extracted from three years old ginseng root of both red skin and normal portion in the same root. After RNA extraction, PCR amplification was performed from cDNA using many random primers. As a result, specific band for red skin was found. It is considered that the gene forming band has possibility to be related with red skin disease, and this gene should be decided if it's related with red skin disease. If that gene is related with red skin disease, it will be used for transformation to foster for resistance to red skin disease as well as for selection marker. Bowever, if it's not related with red skin disease, more primers should be used to find gene related with red skin disease.

• Journal of Periodontal and Implant Science
• /
• v.23 no.1
• /
• pp.159-169
• /
• 1993

The thirty six mandibular second molars, which were extracted because of hopeless tooth due to advanced periodontal disease, were measured the length of mesial and distal root and the distance from cementoenamel junction to root separation. The molars were cross-sectioned every 1.5 milimeter from cementoenamel junction to root apex perpendicular to long axis and each section was photographed, projected and measured with a calibrated Digital Curvi-Meter(Com Curvi-8. Japan). The root surface area (RSA), percentage of the RSA and the linear variation of the RSA were calibrated for each 1. 5 mm section. The results were as follows. 1. The mean length of the roots was 12. 98mm for mesial root, 11.84 mm for distal root. The mesial root was longer than distal root.(p<0.01) 2. The mean distance from the cementoenamel junction to the point at which the root separate from the root trunk was 3.82mm for the buccal furcation and 4.75mm for lingual furcation. The buccal root separation was coronal than the lingual root separation.(p<0.01) 3. The total root surface area was $317.78mm^2$. 4. The mean surface area of the root trunk was $150.06mm^2$ and averaged 42.54% of the total root surface area. 5. The mean root surface area was $88.79\;mm^2$ for the mesial root, $78.93mm^2$ for distal root, The mesial root surface area was wider than the distal root surface area.(p<0.05) 6. In comparision, the mean root trunk surface area of the mandibular 2nd molar was wider than that of mandibular 1st molar(p<0.01), but each root of 2nd molar was smaller than that of 1st molar(p<0.01).

• The Plant Pathology Journal
• /
• v.35 no.6
• /
• pp.692-697
• /
• 2019

Agrobacterium rhizogenes-mediated transformation of sugar beet hairy roots expressing single-chain variable fragment (scFv) was exploited to evaluate the efficacy of four antibody-based constructs for interfering with the Beet necrotic yellow vein virus infection. The scFv specific to a major coat protein of virus, p21, was targeted to various cellular compartments including the cytosol (pIC and pICC constructs), apoplast (pIA), and mitochondrion (pIM). After mechanical virus inoculation, most of the hairy root clones expressing scFv in the cytosol displayed low virus titers while the majority of transgenic hairy root clones accumulated antibody in outer membrane of mitochondria or apoplast were infected. This hairy root system provided an efficient and rapid approach to initially investigating root disease resistance like rhizomania prior to transform whole recalcitrant plants such as sugar beet.

• Plant Resources
• /
• v.4 no.2
• /
• pp.65-74
• /
• 2001

Coleus forskohlii Briq, of the family Lamiaceae yields a valuable secondary metabolity known as forskolin which is a labdane diterpenoid.. Coleus forskohlii is the only known source of this compound. Forskolin is used in medicine for the treatment of glaucoma, congestive cardiomyopathy and asthma. Morphogenic callus was induced from young leaves on MS medium augmented with NAA and BA. These calli, when subcultured on MS with KN alone gave rise to shoots. The regenerated shoot developed good root system on MS medium fortified with NAA. The fully grown plantlets were transferred to soil for acclimatization. Coleus plant is mainly infected by a fungi Lasiodiplodia theobromae which causes root rot disease. The fungal culture filterate (ECE) of Lasiodiplodia theobromae, has been used in regeneration media to find the MIC and further to select resistant plants to the pathogen. In the present study 40% ECF in the medium showed maximum inhibition and is there fore considered as the MIC level of Coleus forshohlii. This data could prove to be useful for the future for selecting a resistant C.forskohlii plant against the root disease caused by L. theobromae.

• Research in Plant Disease
• /
• v.8 no.4
• /
• pp.245-249
• /
• 2002

Monosporascus cannonballus, a soilborne ascomycetes is recently described in Korea that causes root rot/vine decline of cucurbits. The effect of Monosporascus root rot disease on photosynthetic activity and growth was studied on oriental melon plants. At harvest stage, photosynthetic activity of diseased oriental melon plants was lower and stomatal resistance was higher than healthy plants, while xylem exudates were not observed in diseased plants. There was no difference in mineral contents of the leaves and stems between diseased and healthy plants. Leaf area, fresh and dry weights, and fruit weights of the plants were markedly decreased in diseased plants compared to those of healthy plants.

• The Plant Pathology Journal
• /
• v.19 no.4
• /
• pp.195-199
• /
• 2003

Pink root of onion occurred in the fields of the Onion Experimental Station and in the main onion cultivation area in Korea in 1998 and 1999, respectively. The casual fungus of pink root was isolated only from apricot agar. Formation of pycnidia and pycnidiospores of the fungus was highest in alternating cycles of 12 hours near ultraviolet light and 12 hours in dark condition. Its morphological characteristics and pigment formation on water agar were identical with that of Pyrenochaeta terrestris. The optimum temperature for the growth of the fungus and disease development was $25-28^{\circ}C$. When onion seeds were inoculated with the spore suspension, incubated in test-tube and sown in potted soil, disease symptoms developed in onion roots 7 and 30 days after inoculation.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2000.10a
• /
• pp.96-113
• /
• 2000

Coleus forskohlii Briq. of the family Lamiaceae yields a valuable secondary metabolite known as forskolin which is a labdane diterpenoid. Coleus forskohlii is the only known source of this compound. Forskolin is used in medicine for the treatment of glaucoma, congestive cardiomyopathy and asthma. Morphogenic callus was induced from young leaves on MS medium augmented with NAA and BA. These calli, when subcultured on MS with KN alone gave rise to shoots. The regenerated shoot developed good root system on MS medium fortified with NAA. The fully grown plantlets were transferred to soil for acclimatization. Coleus plant is mainly infected by a fungi Lasiodiplodia theobromae which causes root of disease. The fungal culture filterate (FCF) of Lasiodiplodia theobromae, has been used in regeneration media to find the MIC and further to select resistant plants to the pathogen. In the present study 40% FCF in the medium showed maximum inhibition and is there fore considered as the MIC level of Coleus forskohlii. This data could prove to be useful for the future for selecting a resistant C,forskohlii plant against the root disease caused by L.theobromae.