• Title/Summary/Keyword: root development

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Development of Schizogenous and Lysigenous Aerenchyma in Rice Root

  • Kang, Si-Yong;Wada, Tomikichi;Choi, Kwan-Sam
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.2
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    • pp.77-82
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    • 1998
  • Aerenchyma development in rice (Oryza sativa L.) roots is quite important for adaptation to waterlogged or reduced soil conditions. Anatomical observations were carried out to clarify the development of schizogenous and lysigenous aerenchyma in elongating crown roots of rice. The crown roots of 3rd and 4th phytomer were taken from rice plants of the 8th leaf stage grown by hydroponic culture. The schizogenous intercellular spaces in the cortex of crown root tip were observed using a light microscope with semi ultra-thin sections and the lysigenous aerenchyma in mature tissue of crown root were observed using a cryo scanning electron microscope (cryo-SEM) with freezing fracture method. The schizogenous intercellular spaces in the root tip exist obviously in the middle portion of cortical cell layers close to the root-root cap junction, but not in root cap, stele and outer cell layers of cortex. The air spaces were formed at the junction of four neighbouring cells of inner cortex in the transverse sections, and between longitudinal cell layer connected along the root axis. Although many of those spaces were filled with liquid, some spaces seem to exist as air spaces. The lysigenous aerenchyma in the cortex, which hardly filled with liquid, emerged at 3-4 cm segment from the root tip and increased toward the basal region of root axis. The developing process of lysigenous aerenchyma was primarily separation of a radial row of cells caused by the shrinking and collapsing of cortical cells and then formation of septa along the radial cell rows by the fusion of cell wall with each other. These results suggest that the schizogenous and lysigenous aerenchyma playa role as a passage for the movement of oxygen into the root tip region where oxygen is required for respiration.

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Survey of Field Conditions of Clubroot Disease Incidence of Chinese Cabbage in Major Production Areas and Ecology of Root Gall Development (배추무사마귀병 발생실태와 뿌리혹의 생성생태)

  • 김충회
    • Plant Disease and Agriculture
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    • v.5 no.2
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    • pp.77-83
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    • 1999
  • In 1997 surveys 82 out of 180 crucifer fields were infected with clubroot disease in a range of 1-100% of diseased plants and among crucifier crops Chinese cabbage was the most severe, In cropping systems Chinese cabbage-monocropping of Chinese cabbage-radish were found to be most common in major Chinese cabbage production areas. Welsh onion squash or paddy rice were also planted between cropping of Chinese cabbage. Paddy fields converted to upland were lowered in incidence of clubroot disease and fields with loam to silty loam soil were more severe in disease than those with sandy soil. Soil pH and organic contents were nor related to clubroot disease severity. Soil fauua such as total fungi bacteria actinomyces Pseudomonads and Bascillus were not correlated with severity of the disease. Root rall development on Chinese cabbage seedlings was initifially observed under a microscope 13 days after inoculation with Plasmodiophora brassicae but 18 days by naked eyes after inoculation. Root galls were formed mostly around collar roots and gradually spread to main root lateral roots and secondary root branches. Root galls started to enlarge greatly in size and weight from 23 days after inoculation. Chinese cabbage plants at mid-growth stage with root gall development were reduced to 1/2 of that of healthy plants in number of leaves 1/4-1/5 in above ground fresh weight 1/6 in root length but increased to 3 times in diameter of collar root. Diseased plants had little root hairs. Diseased Chinese cabbage plants at harvest were reduced by 9,1-11.8% in head weight compared to healthy plants a positive correlation was observed between root and head weight but those relationships were rot found in the diseased plants.

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Quality evaluation of Angelica gigas Nakai with different drying methods and different root parts (당귀(當歸)의 건조방법 및 뿌리 부위에 따른 품질 평가)

  • Seong, Gi Un;Beak, Mi Eun;Lee, Young Jong;Won, Jae Hee
    • The Korea Journal of Herbology
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    • v.33 no.1
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    • pp.85-91
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    • 2018
  • Objectives : This study was carried out to evaluate the quality of different drying methods and different roots(primary root and lateral root) of Angelica gigas Nakai. Methods : The experimental method was performed according to the Korea Pharmacopoea Eleventh edition (KP11). Loss on drying, ash, acid insoluble ash, ethanol extract, nodakenin and total decursin contents were tested to evaluate the quality of root tissue of Angelica gigas Nakai. In addition, the treatment of different root parts were prepared in two groups of washing dry process and natural dry process. Results : In comparison of dry processing methods, total contents of nodakenin and total decursin in the primary root and lateral root through washing dry process were ranged from 3.55 to 4.09% and from 5.18 to 6.13%, respectively. And also, those of roots from the natural dry process were from 4.36 to 6.22% and from 6.28 to 8.34%, respectively. In the washing dry process and natural dry process methods, 47.9% and 22.3% higher amount of nodakenin and total decursin were measured in lateral root compared to primary root. In common, lateral roots accumulated higher contents of nodakenin and total decursin compared to primary roots, and samples drying processed with natural dry process compared to washing dry process method contained higher amount of compounds. Conclusions : We sincerely hope that this study will be contributed to the standardization and quality control of Angelica Gigas Root.

Chemical Composition of Salicornia Herbacea L.

  • Min, Jin-Gi;Lee, Doo-Seog;Kim, Tae-Jin;Park, Jeong-Heum;Cho, Tae-Yong;Park, Dong-In
    • Preventive Nutrition and Food Science
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    • v.7 no.1
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    • pp.105-107
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    • 2002
  • To get basic data for the utilization of S. herbacea L. as a raw material in food and Chinese herbs, chemical compositions of its leaves, stem and root were investigated. Leaves had the highest level of moisture and the lowest bevel of total sugar. The crude protein and crude lipid contents of the stem were similar to those of the root. Crude ash and salt contents (dry basis) in leaves were considerably higher than those of the stem and root. Total amino acid contents of leaves, stem and root were 1,270 mg/100 g, 1,525 mg/100 g, and 1,569 mg/100 g, respectively. Although the amino acid compositions of loaves, stein, and root were different, their major amino acids were glutamic acid, leuicine, isoleucine and aspartic acid. The rich minerals in leaves, stem and root were Na, K and Ca.

Effects of Vermicompost Application on the Growth and Ginsenoside Content of Panax ginseng in a Reclaimed Field

  • Eo, Jinu;Park, Kee-Choon;Lim, Jin-Soo;Kim, Myung-Hyun;Choi, Soon-Kun;Na, Young-Eun
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.2
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    • pp.138-143
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    • 2016
  • The objective of this study was to evaluate the effects of the application of vermicomposts on the growth parameters and ginsenoside content of ginseng roots. Food waste vermicompost (FW), cow manure vermicompost (CM), and paper sludge vermicompost (PS) were applied at 10 and $40t\;ha^{-1}$, respectively. One-year-old seedlings were transplanted and 4-year-old roots were harvested. Soil nitrate and phosphate concentrations were increased in the plots applied with FW and CM at $40t\;ha^{-1}$. Soil pH and exchangeable Ca concentrations were higher at FW $40t\;ha^{-1}$ than at CM $40t\;ha^{-1}$. Root yield increased when treated with FW $40t\;ha^{-1}$ in comparison to the yield for the control. The incidences of root rot disease and ginsenoside content were not significantly affected by the treatments. The results suggested that application of vermicompost might not show a relationship between root biomass and ginsenoside content. It further showed that proper use of vermicompost can promote root yield without a reduction in root quality or an increase in the incidence of root rot disease in reclaimed fields.

Relationships Between Soil-Borne Virus Infection and Root Growth Damage in Korean Hulless Barley Cultivars

  • Park, Jong-Chul;Jonson, Gilda;Noh, Tae-Hwan;Park, Chul-Soo;Kang, Chon-Sik;Kim, Mi-Jung;Park, Ki-Hoon;Kim, Hyung-Moo
    • The Plant Pathology Journal
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    • v.25 no.3
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    • pp.231-235
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    • 2009
  • Viral infections and root growth were examined to elucidate the relationship between viral resistance and root growth in 26 Korean hulless barley cultivars. Viral resistance was estimated in experimental filed of Honam agricultural research institute for 3 years. Length and number of seminal and adventitious roots were examined for evaluation of root growth in both field and green-house conditions 30 days after seeding. Dominant viral infection occurred in Korean hulless barley by Barley yellow mosaic virus (BaYMV) in fields; however, susceptible cultivars were infected by either BaYMV, Barley mild mosaic virus (BaMMV) or both. Only four cultivars, including Donghanchalssalbori, Kwangwhalssalbori, Namhossalbori and Naehanssalbori, presented stable resistance to viral infections. Susceptible cultivars to viral infection in fields showed shorter seminal root length and fewer adventitious root number than resistant cultivars. Resistant cultivars showed better root growth and significant difference in adventitious root length in green house conditions. Increase in the number of seminal roots in resistant cultivars was derived from decreased damage of roots by the viral infection compared to the susceptible cultivars.

Effect of Soil Phosphorus Levels on Seed Emergence, Seedling Mortality and Plant and Root Development of American Ginseng (토양 중 인산수준이 미국삼 종자출아, 유모결주율 및 식물생육에 미치는 영향)

  • Thomas, S.L.Li;Michael, O.Wallis
    • Journal of Ginseng Research
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    • v.18 no.2
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    • pp.134-136
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    • 1994
  • The effects of soil phosphorus level on seed emergence, seedling mortality, plant and root development of American ginseng (Panax quinquefolium L.) were evaluated in a newly planted commercial ginseng garden. Phosphorus levels were increased from 58 ppm to 100, 150 and 200 ppm with triplephosphate (0-45-0). Higher phosphate levels increased, seed emergence and reduced seedling mortality. Root length, diameter, fresh root weight and total leaflet length were not affected by phosphate levels.

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Biological Control of Mulberry Root Knot Nematode Meloidogyne incognita by Trichoderma harzianum

  • Sukumar, J.;Padma, S.D.;Bongale, U.D.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.8 no.2
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    • pp.175-179
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    • 2004
  • Trichoderma harzianum-THN1 parasitising the egg masses of root knot nematode Meloidogyne incognita was isolated from galled mulberry roots and evaluated for its potential to control root knot disease. In pot experiments root galling was reduced and leaf yield increased significantly following soil treatment with T. harzianum-THN1. The extracts obtained from the soils inoculated with T. harzianum-THN1 drastically inhibited the hatching of nematode eggs and the effect was irreversible even after the eggs were transferred to fresh water. The fungus was equally effective in controlling the disease in nematode infested mulberry garden under field conditions which was significant over the most commonly used egg parasitic fungus Paecilomyces lilacinus. The disease reduction recorded with T. harzianum was on par with the plants treated with the nematicide Carbofuran. The results suggest that T. harzianum- THN1 could be used as a potent ecofriendly biocontrol agent against M. incognita in mulberry without any residual toxicity to silkworms. T. harzianum- THN1 can form an important component of integrated disease management package in mulberry cultivation.

Allelopathy of Tagetes minuta L. Aqueous Extracts on Seed Germination and Root Hair Growth

  • Kil, Ji-Hyun;Shim, Kew-Cheol;Lee, Kyu-Jin
    • The Korean Journal of Ecology
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    • v.25 no.6
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    • pp.395-398
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    • 2002
  • Present paper showed allelopahtic effects of Tagetes minuta aqueous extracts on seed germination and root hair development. Allelopathy of aqueous extracts derived from T. minuta examined using two test plant species (Lotus comiculatus var. japonicus and Lactuca sativa). The seeds of test species were inoculated in petri dishes containing 0, 10,50 and 100% aqueous extracts from T. minuta. At day 5, the relative seed germination ratio to control was evaluated, and the development of seedling root hairs was observed through light microscopy. Seed germination of L. comiculatus var. japonicus was significantly inhibited proportional to the concentrations of aqueous extract, but that of L. sativa wasn't inhibited. The inhibitory allelopathic effect of T. minuta was found in the development and growth of seedling root hairs. It was concluded that the inhibitory allelophatic effects have been to be investigated using various bioassay, for the allelopathy of plant species shows species-specific and organ-specific.

Estimation of the relationship between below-ground root and above-ground canopy development by measuring dynamic change of soil ammonium-N concentration in rice

  • Fushimi, Erina;Yoshida, Hiroe;Tokida, Takeshi;Nakagawa, Hiroshi
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.183-183
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    • 2017
  • In the early part of rice growth, root volume primarily limits the amount of plant-accessible nitrogen (N). Therefore, knowledge of the root development is important for modeling N uptake of rice. The timing when the volume of rhizosphere cover the whole soil is also important to carry out timely top dressing. However, information about initial root expansion and associated N uptake is limited due to intrinsic technical difficulties in assessing below-ground processes. Some studies, however, showed a close relationship between below-ground root and above-ground leaf development, suggesting a possibility that above-ground attributes could serve as surrogates for the root processes. In this study, we investigated the relationship between below-ground and above-ground development of rice. Field experiments were conducted where we cultivated Koshihikari (a leading cultivar in Japan) for four different cropping schedules in 2012. In 2016, Gimbozu (HEG4) and three flowering time mutant lines of Gimbozu (X61 (se13), HS276 (ef7), DMG9 (se13, ef7)) were examined for a single season. Experiments were performed with three replications in a completely randomized design. We monitored ammonium-N concentration ([NH4+-N]) in soil solution by repeatedly taking samples from a porous tubing (10-cm long) vertically inserted at the most distant point from surrounding rice hills. Samples were taken in triplicate (= triplicate tubes) and every three days from transplanting in each experimental unit. For above-ground attributes, leaf area index (LAI) was measured in 2012, whereas soil coverage ratio was estimated by image processing in 2016. Results showed that [NH4+-N] increased gradually after transplanting and then rapidly decreased from a certain day. This distinct drop in [NH4+-N] informed us the timing at which the rice root system reached the point of porous tubing and thus essentially covered the whole soil volume. The LAI at the dropping point was about 0.43 regardless of the cropping schedules in 2012 experiment. In 2016, the coverage ratio at the N dropping point was within the range of 0.12 to 0.19 for four genotypes having different growth durations. In addition, the coverage ratios at seven weeks after the transplanting showed a good correspondence to LAI across the four genotypes. We therefore conclude that both LAI and coverage ratio may serve as robust indicators for root development and might be useful to estimate the timing when the root system fully cover the soil volume. Results obtained here will also contribute to develop models that can predict not only above-ground canopy development but also associated below-ground processes.

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