• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.1
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• pp.92-97
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• 2005

Inhibition of inflammatory response, acceleration of basal cell growth, and balanced synthesis of extracellular matrix (ECM) are important in healing of cutaneous open wounds. In order to evaluate the healing effects of water extracts of Radix Astragali (the root of Astragalus membranaceus (Fisch.)) on open wound at early stage, the experimental open wounds were generated on the dorsal sides of SD rats under anesthesia. The boiled-water extracts of Radix Astragali $(100{\mu}l)$, soaked into an occlusive film dressing were applied once a day for eleven consecutive days. The healing process was assessed by measuring macroscopic appearance and wound areas of the open wounds. The molecular aspects of healing process by Radix Astragali extracts were also investigated by Hematoxylin-Eosin (H-E) double staining and immunohistological staining of collagen type I in the healed skin area, implying cell density and linear alignment of the granulation tissue, and ECM synthesis and its remodeling, respectively. The Astragali radix extracts were found to significantly accelerate the cutaneous wound healing by suppressing the inflammation and stimulating the basal cell growth in wounded area, as compared to epidermal growth factor (EGF).

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.2
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• pp.92-97
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• 1983

This experiment was designed to establish the soil physical improvement practice to increase the rice yields at fine textured fluvio-marine paddy field. After chiseling and trenching the paddy soil, the rice growth and soil physical properties were investigated for 4 years. The results are summarize as follows; 1. The soil physical properties, such as bulk density, hardness, porosity and permeability were prominently improved by trenching and chiseling. 2. There were significant relationships among the soil physical properties, root developments and rice yields. 3. The increasing rates of rice yields by soil physical improvement were about 10%, which is better than 5% of drainage effects. 4. The effects of trenching and chiseling on rice yields were continued to fourth year. Even if trenching effects was better than chiseling the chiseling was considered as a useful treatment for its simple working practice by using a heavy machine. 5. In case of trenching, straw application were increased the rice yields.

• Journal of Bio-Environment Control
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• v.22 no.3
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• pp.228-233
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• 2013

This study was conducted to examine the effect of light intensity and photoperiod of white LEDs as the artificial light source on the growth of leaf lettuce (Lactuca sativa L.) 'Seonhong Jeokchukmyeon' in a closed-type plant production system. Seedlings, transplanted at a density of $20cm{\times}20cm$ in a completely randomized design, were grown under white LEDs (FC Poibe Co. Ltd., Korea), at one of the 3 light intensities (100, 200, or $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$), and each with one of 3 photoperiods [12/12, 18/6, or 24/0 (Light/Dark)]. Plants were cultured for 22 days under the condition of $21{\pm}2^{\circ}C$, $60{\pm}10%$ RH, and $400{\pm}50{\mu}mol{\cdot}mol^{-1}\;CO_2$. The greatest leaf length and width, fresh and dry weights, and total anthocyanin content were obtained in the 24/0 photoperiod, regardless of the light intensity. Length of the longest root, fresh and dry weights, and number of leaves were greater in light intensity of $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ than 100 or $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Chlorophyll value was the greatest in the photoperiod 12/12 than 18/6 or 24/0. The results obtained suggest that plant grew the best kept by light intensity at 200 or $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, and photoperiod of 12/12 or 18/6.

• Journal of Bio-Environment Control
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• v.29 no.1
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• pp.1-8
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• 2020

The influence of growth regulators (NAA and BA) and sucrose concentrations (0, 3, 5, 7, 9%) on in vitro rapid-propagation of virus-free sweet potato [Ipomoea batatas (L.) Lam.] was investigated with single-node or shoot-tip culture of two cultivars ('Matnami' and 'Shinhwangmi'). The survival rate and growth of shoot-tip explant was also investigated under the presence or absence of light (blue and red LED = 7:3, 150±5 μmol·m^-2·s^-1 PPFD) during minimal-growth in vitro conservation at 15℃. Vine length, vine diameter, fresh weight and dry weight were enhanced without callusing of explant in the MS medium supplemented with 0.2-0.5 mg·L^-1 BA. The growth of single-node and shoot-tip explants were significantly enhanced with the increase of vine length, number of leaf, number of root, fresh weight, and dry weight in the solid medium containing 5% sucrose and 0.2 mg·L^-1 BA. Vine elongation of shoot-tip explants were highest in the liquid medium containing 3% sucrose than the solid medium. The survival rate of minimal-growth in vitro conservation was 100% in 5 months under the presence of light (LED, 150±5 μmol·m^-2·s^-1 PPFD) at 15℃, but the explants in dark condition died in 3 months. The light was absolutely necessary for the in vitro conservation under minimal-growth conditions of virus-free sweet potato plantlets at 15℃, and the high density of explants (10 plantlets per Petri Dish) was increased the efficiency of mass conservation.

• Asian Journal of Turfgrass Science
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• v.25 no.1
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• pp.106-111
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• 2011

This study was conducted to investigate the effect of the mixture ratio of a soldier fly casts (SFC), compost and cocopeat on the soil physicochemical properties. The mixture ratios of soil amendment were 0%, 3%, 5%, 7% and 10% (V/V) incorporated with sand which met to the USGA particle standard. To analyze the effects of amendments on soil chemical properties, pH and EC were measured. The porosity, capillary porosity, air-filled porosity, bulk density and hydraulic conductivity also measured to analyze the physical properties. Chemical properties were significantly different by mixture ratios of a SFC, compost and cocopeat. Capillary porosity was a factor involved in soil physical properties by blending with a SFC and compost. It was affected on the volume of porosity or hydraulic conductivity. To analyze the correlation of mixture ratio versus to physical characters, the ratios of SFC were significantly different in capillary porosity, air-filled porosity, and hydraulic conductivity. These results indicated that mixing ratios of SFC were affected on soil physicochemical properties such as porosity and hydraulic conductivity of the root zone on the USGA sand green.

• Journal of Bio-Environment Control
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• v.14 no.1
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• pp.52-55
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• 2005

An experiment was conducted to evaluate the efficacy of continuous lateral shoot pinching and gradual adjustment of drip irrigation lines in improving plant health as well as fruit yield and quality in white-spined cucumber grown in the greenhouse. Grafted white-spined cucumber seedlings were established at a density of 18,000 plants per ha in a greenhouse. Main stems were pinched at 21st node. Each of the lateral shoots was pinched at the 3rd node and 2-3 fruits per lateral shoot were harvested. Drip irrigation lines were gradually adjusted to provide water to the area of maximum root concentration. Plants grown with these combined treatments produced $55\%$ more lateral shoots and 38% more marketable yield, as compared to the control.

• Korean Journal of Plant Resources
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• v.31 no.5
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• pp.515-521
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• 2018

This was carried out to develop a chromosome-doubled (12x) persimmon that will be used as a crossing parent to select seedless persimmon cultivars with the change of the consumption trend recently. To obtain a chromosome-doubled (12x) persimmon, colchicine was applied at the meristem of seedlings in vitro derived from cross among hexaploid persimmon (Diopyros kaki Thunb.). These were treated with 0.03%, 0.05% and 0.1% colchicine respectively for doubling chromosome, and it was most effective at the concentration of 0.05% colchicine. After colchicine treatment, we conducted tests to elucidate conditions for inducing shoot and root development. As the result, the shoots grew best when cultivated at 1/2MS media plus 10 and $30{\mu}M$ zeatin respectively, and the roots grew best when cultivated at 1/2MS media after dipping for 5 seconds at 10 mM NAA+5% DMSO. We also compared seedlings that have chromosome (6x) do not doubled and crossing parents (6x) and chromosome-doubled seedlings (12x). As the result, these chromosome-doubled seedlings (12x) showed lower stomatal density and larger stomatal size.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.225-225
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• 2017

This study is to investigate the effect of split application and adapted fertilization application cultivation in stable medicinal herbs production of Acorus graminens S. And it accomplished one's purpose to GAP guide manual established standard method of cultivation technology, contribute to the enlargement of farm's income. Method of fertilization application were conducted under five condition compose to standard application(N-P-K-Compost: fermented mixed oil cake fertilizer applied at $4-2-6-300kg.10a^{-1}$, twofold application(N-P-K-Compost applied at $8-4-12-600kg.10a^{-1}$) cultivation. Compost (fermented mixed oil cake fertilizer) and fused phosphate applied at 100% of basal fertilizer in April 14. The split application level of standard application and twofold application(N-K) applied at 100%, 50%, 40% of basal fertilizer in April 14, 50% and 40% of top dressing were three times application (N-K) in June 1, July 15, August 15, 25% and 20% respectively. Transplanting with Naju varieties in May 12 by growing pot seedling for 40 days in 2015. Planting density were spaced 30 cm apart in rows 30 cm apart with black vinyl mulching on open cultivation. Plot design randomized block 3 repetition. Therefore, growth characteristics by 50% and 40% of top dressing were three times application (N-K) compared to standard application and twofold application (N-K) applied at 100% basal fertilizer, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., Length of diameter and main root of subterranean part growth increment rising highest. Dried roots yields treated standard fertilizer 100% application($250kg.10a^{-1}$) compared to 100% from twofold application (N-K) applied at 50% basal fertilizer, 50% and 40% of top dressing were three times application increased by 16%, 20%. respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.2
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• pp.140-145
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• 1997

The change of physico-chemical properties of paddy soil was monitored under different tillage. This study was conducted on Jeonbug silty cly loam, in Honam Agricultural Experiment Station, for four years starting from 1992. Different tillage involved (1)Continued no tillage, (2)Rotavation only with tractor every year, (3)Spring plowing with power tiller plus rotavation with tractor every year. The result of this study can be summarized as: Non tillage, when continued for some years, tended to increase 1)the hardness of soil, 2)the root density in the top soil, 3)the occurrence of annual and perennial weeds, and tended to lower the yield of rice compared to normal tillage(tilling with ow tiller in the spring plus rotavation by tractor before planting.

• Korean Journal of Soil Science and Fertilizer
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• v.25 no.1
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• pp.77-88
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• 1992

Effective strains of cowpea bradyrhizobia JB7 $nal^rspe^r$ and CB756 $str^rrif^r$, antibiotic-resistant variants of JB7 and CB756, respectively, were used to examine changes of rhizosphere populations and nodule occupancy. Populations of each strain increased gradually in the rhizosphere, reaching a maximum of about $10^8$ cells per root system. Nodule number increased as the density of inoculum increased from $10^2$ cells to $10^8$ cells per seed. Inoculation with liquid suspension resulted in the formation of more nodules than the peat slurry or granule inoculation. When JB7 $nal^rspe^r$ and CB756 $str^rrif^r$ were introduced in equal numbers in inoculum mixtures the former consistantly occupied the majority of nodules with all three groundnut cultivars used. There was no difference in yield between nitrogen treatments and inocultation treatments.