• Title/Summary/Keyword: root cultures

Search Result 272, Processing Time 0.026 seconds

A Study on the Modern 'Universal Philosophy' Idea-Presentation of 'Avant-garde' Art Groups at the Turn of the 20th's Century - On the Progress of the Philosophies, 'Universalism' as a Intellectual Synthesis toward Awakening for Modern Art - (20세기 전환기의 '아방가르드' 예술집단의 근대 '보편주의' 사상-표현에 관한 연구 -근대 예술적 자각을 향한 지적 융합, 보편철학의 발전적 전개-)

  • Oh, Zhang-Huan
    • Journal of architectural history
    • /
    • v.17 no.6
    • /
    • pp.87-98
    • /
    • 2008
  • The purpose of this study is ultimately subjected to the Orientalism, even though this deals with some positive effects in the realm of art and architecture as the scope of study, because through which the relationship between two different cultures will be discussed. That is to say, this research focused not only on how the presentation of 'avant-garde' visual art, which is explained as formal 'purity' and 'abstraction' as the characteristics of modern arts, could be made in the transition to the 20th's World, but also on what is the role and meaning of Eastern thoughts, which is popular in that time, for the new philosophical background of the artistic revolution. As a result, this study found that a lot of 'avant-garde' architects such as F. L. Wright, M. Mahony in Prairie School and L. Sullivan, D. Burnham, J. Root in Chicago School, and Lauweriks, H. P. Berlage who introduced Wright's works into the Europe, had possessed the 'Universal Philosophy' including Unitarianism, Transcendentalism, Deism, and Theosophy which are all influenced by Oriental religions and thoughts through historic western philosophers, although it is generally well-known that W. Kandinsky and P. Mondrian were belong to that. Furthermore, they gave attention to the Oriental religions and thoughts in that time, eventually made a historical progressive process of unification of thoughts between East and West. In a word, the new universalism was the philosophical background that made the artist's idea and presentation on 'from Being into Becoming'.

  • PDF

Gene Manipulation of Pin 2(Proteinase Inhibitor II) to the Cottonwood Leaf Beetle(Coleoptera : Chrysomelidae) in Transgenic Poplar(Populus deltodies × P. nigra) (형질전환(形質轉換)된 포플러의 딱정벌레에 대한 저항성(抵抗性) 유전자(遺傳子)(Proteinase Inhibitor II) 발현(發現))

  • Kang, Hoduck
    • Journal of Korean Society of Forest Science
    • /
    • v.86 no.4
    • /
    • pp.407-414
    • /
    • 1997
  • The resistance of a non-transgenic poplar clone, 'Ogy' and three transgenic poplar lines to the cottonwood leaf beetle, Chrysomela scripta F., was evaluated by in vitro feeding. The lines were transformed with neomycin phosphotransferase II(NPT II) as a selectable marker, proteinase inhibitor II(pin2) as a resistance gene, and CaMV 35S as a promoter. An efficient method of sterilizing the beetle eggs and introducing them into plant tissue cultures was developed. The resistance of the transgenic lines was investigated in terms of effects tin leaf area consumed, insect weight, insect developmental stages, and plantlet root dry weight after feeding. Also, leaf area consumed was examined by leaf age as measured through leaf plastochron index(LPI). The leaf area consumed and insect weight were highly significant between transformants and control, and insect development in vitro was significant among the transgenic lines. Larval infestation was the most severe around LPI 4 to 5 which were young leaves. The system provided a quick, highly controlled method to screen developing transgenic plantlets directly.

  • PDF

In vitro propagation from axillary bud explants of Fatsia japonica Deene. et Planeh (팔손이 액아배양을 통한 기내증식)

  • Choi, Kyung-Mi;Hwang, Sung-Jin;Ahn, Jun-Cheul;Lee, Hyeon-Yong;Kim, Jae-Heun;Hwang, Baik
    • Korean Journal of Medicinal Crop Science
    • /
    • v.13 no.6
    • /
    • pp.300-303
    • /
    • 2005
  • A propagation for Fatsia japonica using axillary bud explants were established. Cultures were initiated from axillary bud explants on MS medium supplemented with IAA $(1,2,3\;mgl^{-1})$, 2,4-D $(1,2,3\;mgl^{-1})$ or NAA $(1,2,3\;mgl^{-1})$ in combination with BA $(0.5\;mgl^{-1})$. The maximum shoot bud formation was obtained in MS medium supplemented with $0.5\;mgl^{-1}$ BA and $2;mgl^{-1}$ IAA after 4 weeks culture. The microshoot rooted within 4 week in MS medium containing $1.0\;mgl^{-1}$ IBA.

Micropropagation of a Rare Tree Species, Empetrum nigrum var. japonicum K. Koch via Axillary Bud Culture (희귀 수종 시로미의 액아줄기 유도 미세번식)

  • Han, Mu-Seok;Park, So-Young;Moon, Heung-Kyu;Kang, Young-Jae
    • Journal of Korean Society of Forest Science
    • /
    • v.99 no.4
    • /
    • pp.568-572
    • /
    • 2010
  • In order to develop an efficient micropropagation system for a rare tree species, Empetrum nigrum var. japonicum K. Koch, the effect of medium salt, cytokinins and auxin at different concentration were evaluated. Shoot induction from axillary bud was better on WPM medium than on MS medium. Although there was no significant differences observed in shoot induction among the salt strengths of WPM medium, whereas healthy shoots were developed on basal WPM medium. In comparison of the cytokinins affecting shoot proliferation, zeatin was better than BA, whereas BA exhibited more effectiveness on shoot elongation. In vitro root formation was better on WPM medium than on 1/2MS medium and achieved the highest rooting rate when 5.0 mg/L IBA treatment. 93% of rooted plantlets were survived on artificial soil mixture after 4 weeks of acclimatization. Above results suggest that a rare tree species, E. nigrum var. japonicum can be micropropagated via axillary bud cultures.

Phytotoxicity of dehydrofusaric acid isolated from Fusarium oxysporum against several plants (Fusarium oxysporum이 생산하는 dehydrofusaric acid의 몇 가지 식물 생육에 대한 활성)

  • Hong, Kyung-Sik;Choi, Gyung-Ja;Kim, Heung-Tae;Hwang, In-Taek;Cho, Kwang-Yun;Kim, Jin-Cheol
    • The Korean Journal of Pesticide Science
    • /
    • v.6 no.3
    • /
    • pp.202-208
    • /
    • 2002
  • A phytotoxin was purified by repeated chromatography from liquid cultures of Fusarium oxysporum BG isolated from barnyardgrass. Its chemical structure was determined to be dehydrofusaric acid by mass and NMR spectral analyses. The substance showed a potent phytotoxic activity against growth of duckweed with a $EC_{50}$ value of $1.5{\mu}g/ml$. It also inhibited the root growth of barnyard millet, cress, barnyard grass, and rice cultivar 'Dongjin'. However, it had no inhibitory activity against seed germination of barnyard millet and cress, and the shoot growth of the four plant species.

Ginseng extracts modulate mitochondrial bioenergetics of live cardiomyoblasts: a functional comparison of different extraction solvents

  • Huang, Yun;Kwan, Kenneth Kin Leung;Leung, Ka Wing;Yao, Ping;Wang, Huaiyou;Dong, Tina Tingxia;Tsim, Karl Wah Keung
    • Journal of Ginseng Research
    • /
    • v.43 no.4
    • /
    • pp.517-526
    • /
    • 2019
  • Background: The root of Panax ginseng, a member of Araliaceae family, has been used as herbal medicine and functional food in Asia for thousands of years. According to Traditional Chinese medicine, ginseng is the most widely used "Qi-invigorating" herbs, which provides tonic and preventive effects by resisting oxidative stress, influencing energy metabolism, and improving mitochondrial function. Very few reports have systematically measured cell mitochondrial bioenergetics after ginseng treatment. Methods: Here, H9C2 cell line, a rat cardiomyoblast, was treated with ginseng extracts having extracted using solvents of different polarity, i.e., water, 50% ethanol, and 90% ethanol, and subsequently, the oxygen consumption rate in healthy and tert-butyl hydroperoxideetreated live cultures was determined by Seahorse extracellular flux analyzer. Results: The 90% ethanol extracts of ginseng possessed the strongest antioxidative and tonic activities to mitochondrial respiration and therefore provided the best protective effects to H9C2 cardiomyocytes. By increasing the spare respiratory capacity of stressed H9C2 cells up to three-folds of that of healthy cells, the 90% ethanol extracts of ginseng greatly improved the tolerance of myocardial cells to oxidative damage. Conclusion: These results demonstrated that the low polarity extracts of ginseng could be the best extract, as compared with others, in regulating the oxygen consumption rate of cultured cardiomyocytes during mitochondrial respiration.

Endophytic Fungi of Salt-Tolerant Plants: Diversity and Ability to Promote Plant Growth

  • Khalmuratova, Irina;Choi, Doo-Ho;Kim, Jong-Guk;Lee, In–Seon
    • Journal of Microbiology and Biotechnology
    • /
    • v.31 no.11
    • /
    • pp.1526-1532
    • /
    • 2021
  • Suaeda australis, Phragmites australis, Suaeda maritima, Suaeda glauca Bunge, and Limonium tetragonum in the Seocheon salt marsh on the west coast of the Korean Penincula were sampled in order to identify the endophytes inhabiting the roots. A total of 128 endophytic fungal isolates belonging to 31 different genera were identified using the fungal internal transcribed spacer (ITS) regions and the 5.8S ribosomal RNA gene. Fusarium, Paraconiothyrium and Alternaria were the most commonly isolated genera in the plant root samples. Various diversity indicators were used to assess the diversity of the isolated fungi. Pure cultures containing each of the 128 endophytic fungi, respectively, were tested for the plant growth-promoting abilities of the fungus on Waito-C rice germinals. The culture filtrate of the isolate Lt-1-3-3 significantly increased the growth of shoots compared to the shoots treated with the control. Lt-1-3-3 culture filtrate was analyzed and showed the presence of gibberellins (GA1 2.487 ng/ml, GA3 2.592 ng/ml, GA9 3.998, and GA24 6.191 ng/ml). The culture filtrate from the Lt-1-3-3 fungal isolate produced greater amounts of GA9 and GA24 than the wild-type Gibberella fujikuroi, a fungus known to produce large amounts of gibberellins. By the molecular analysis, fungal isolate Lt-1-3-3 was identified as Gibberella intermedia, with 100% similarity.

Propagation by In Vitro Zygotic Embryos Cultures of the Quercus myrsinifolia

  • Choi, Eun ji;Yong, Seong Hyeon;Seol, Yu Won;Park, Dong Jin;Park, Kwan Been;Kim, Do Hyun;Jin, Eon Ju;Choi, Myung Suk
    • Journal of Forest and Environmental Science
    • /
    • v.37 no.4
    • /
    • pp.323-330
    • /
    • 2021
  • Zygotic embryo culture was performed to propagate evergreen oak, Quercus myrsinifolia, which has recalcitrant seeds and is difficult to propagate by cuttings. Zygotic embryos appeared in WPM medium after 14 days, and after 56 days, they developed into complete plants with cotyledons and roots. The medium suitable for zygotic embryo culture was 1/4 WPM medium, showing a shoot growth of 2.43 cm and root growth of 8.7 cm after 8 weeks of culture. As a result of investigating the effect of GA3 on the growth of plants germinated from zygotic embryos through GA3 treatment, the best growth was shown in 0.5 mg/l GA3 treatment. The in vitro rooting and growth of IBA-treated zygotic embryo-derived plants were good in the 0.5 mg/l IBA treatment and rooting and shoot growth were not observed at higher concentrations. And the callus induction rate also increased as the concentration of IBA increased. Plants grown in vitro were transferred to a plastic pot containing artificial soil and acclimatized in a greenhouse for about 4 weeks, resulting in more than 90% survival. As a result of this study, the zygotic embryo culture method was confirmed to be effective for mass propagation of Q. myrsinifolia. The results of this study are expected to contribute significantly to the mass propagation of elite Q. myrsinifolia.

Tissue-cultured regeneration and ecological values in major bamboo species

  • Sharma, Avinash;Manpoong, Chowlani;Gohain, Anwesha;Pandey, Himanshu;Padu, Gompi;Aku, Hage
    • Journal of Ecology and Environment
    • /
    • v.46 no.3
    • /
    • pp.218-242
    • /
    • 2022
  • Background: Promising specific growth regulators are employed in the tissue cultures of various bamboo species. Specific natural hardening mixtures support the acclimatization and adaptation of bamboo under protected cultivation. Results: The growth regulators like 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Naphthaleneacetic Acid (NAA), Thidiazuron (TDZ), 6-Benzylaminopurine (BAP), Kinetin, Gelrite, Benzyl Adenine (BA), Indole Butyric Acid (IBA), Coumarin, Putrescine, Gibberellic acid (GA3), Indole Acetic Acid (IAA) has been widely used for callus induction, root regeneration and imposing plant regeneration in various species of bamboo such as Bambusa spp. and Dendrocalamus spp. Different combinations of growth regulators and phytohormones have been used for regenerating some of the major bamboo species. Natural hardening materials such as cocopeat, vermicompost, perlite, cow dung, farmyard manure, compost, soil, garden soil, and humus soil have been recommended for the acclimatization and adaptation of bamboo species. Standard combinations of growth regulators and hardening mixtures have imposed tissue culture, acclimatization, and adaptation in major bamboo species. Conclusions: Bamboo contributes to soil fertility improvement and stabilization of the environment. Bamboo species are also involved in managing the biogeochemical cycle and have immense potential for carbon sequestration and human use. This paper aims to review the various growth regulators, natural mixtures, and defined media involved in regenerating major bamboo species through in vitro propagation. In addition, the ecological benefits of safeguarding the environment are also briefly discussed.

Isolation of Indole-3-acetic acid (IAA) producing Arthrobacter sp. and plant growth promotion effect (Indole-3-acetic acid (IAA) 생성 Arthrobacter sp.의 분리 및 식물 생육촉진 효과)

  • Da Som Kim;Ho-Young Shin;Song-Ih Han
    • Journal of the Korean Applied Science and Technology
    • /
    • v.39 no.6
    • /
    • pp.831-838
    • /
    • 2022
  • An auxin-producing bacteria, KSD16, KSD33, and KSD36 were isolated from agricultural soil. The strain KSD16, KSD33, and KSD36 was classified as a strain of Arthrobacter sp. based on phylogenetic analysis of 16S rRNA gene. The isolated KDS16, KDS33, and KSD36 was confirmed to produce indole-3-acetic acid (IAA), which is one of the auxin hormones. When the concentration of IAA was assessed the maximum concentration of IAA, 206.62 mg L-1, was detected from the culture broth incubated in R2A medium containing 0.1% L-tryptophan for 48 h at 28 ℃. To study the effect of IAA producing bacteria on germination rate, seeds of Mung bean were prepared for each treatment. KSD16, KSD33, and KSD36 showed significant increase in root length and number of adventitious roots than the controls. To investigate the growth-promoting effects on the crops, Arthrobacter species were placed in water cultures and seed pots of mung beans. In consequence, the seed germination of mung beans was 73.4% higher than the control.