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http://dx.doi.org/10.12925/jkocs.2022.39.6.831

Isolation of Indole-3-acetic acid (IAA) producing Arthrobacter sp. and plant growth promotion effect  

Da Som Kim (Department of Microbial Biotechnology, Mokwon University)
Ho-Young Shin (Department of Microbial Biotechnology, Mokwon University)
Song-Ih Han (Department of Microbial Biotechnology, Mokwon University)
Publication Information
Journal of the Korean Applied Science and Technology / v.39, no.6, 2022 , pp. 831-838 More about this Journal
Abstract
An auxin-producing bacteria, KSD16, KSD33, and KSD36 were isolated from agricultural soil. The strain KSD16, KSD33, and KSD36 was classified as a strain of Arthrobacter sp. based on phylogenetic analysis of 16S rRNA gene. The isolated KDS16, KDS33, and KSD36 was confirmed to produce indole-3-acetic acid (IAA), which is one of the auxin hormones. When the concentration of IAA was assessed the maximum concentration of IAA, 206.62 mg L-1, was detected from the culture broth incubated in R2A medium containing 0.1% L-tryptophan for 48 h at 28 ℃. To study the effect of IAA producing bacteria on germination rate, seeds of Mung bean were prepared for each treatment. KSD16, KSD33, and KSD36 showed significant increase in root length and number of adventitious roots than the controls. To investigate the growth-promoting effects on the crops, Arthrobacter species were placed in water cultures and seed pots of mung beans. In consequence, the seed germination of mung beans was 73.4% higher than the control.
Keywords
auxin; Arthrobacter sp.; indole-3-acetic acid; plant growth promotion; seed germination;
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1 D.B. Brana, P.T. Lacava, A. Ferrari, N. S. Teixeira-silva, "M.L. Bonatelli, et al. Screening of tropically derived, multi-trait plant growth- promoting rhizobacteria and evaluation of corn and soybean colonization ability", Microbiol Res. Vol.206, pp.33-42, (2018).    DOI
2 Y. Jiang, Y. Song, C. Jiang, X. Li, T. Liu, J. Wang, C. Chen, J. Gao. "Identification and Characterization of Arthrobacter nicotinovorans JI39, a Novel Plant Growth-Promoting Rhizobacteria Strain From Panax ginseng", Frontiers in Plant Science, 13: 873621, (2022). 
3 M. Lambrecht, Y. Okon, A. Broek, J. Vanderleyden. "Indole-3-acetic acid: a reciprocal signalling molecule in bacteria-plant interactions", Trends in microbiology. Vol.8, No.7, pp.298-300, (2000).    DOI
4 M. R. Sunayanal, Ch. Sasikala, and Ch. V. Ramanal. "Rhodestrin: a novel indole terpenoid phytohormone from Rhodobacter sphaeroides", Biotechnology Letters. Vol.27, pp.1897-1990, (2005).    DOI
5 B. Mahadevan, and D. L. Crawford. "Properities of the chitinase of the antifungal biocontrol agent Streptomyces lydicus WYEC108", Enzyme and Microbial Technology. Vol.20, pp.489-493, (1997).    DOI
6 M. Sunayanal, C. Sasikala, and C. Ramanal. "Rhodestrin: a novel indole terpenoid phytohormone from Rhodobacter sphaeroides", Biotechnology Letters. Vol.27, pp.1897-1990, (2005).    DOI
7 de Vasconcellos, R. L. F. and E. J. B. N. Cardoso. "Rhizoꠓspheric Streptomycetes as potential biocontrol agents of Fusarium and Armillaria pine rot and as PGPR for Pinus taeda", Biocontrol. Vol.54, pp.807, (2009). 
8 J. Y. Yoo, H. H. Lee, C. H. Han, and M. H. Yoon. "Characterization of auxin production plant growth promotion by a bacterium isolated from button mushroom compost", Journal of Mushroom. Vol.15, No.1, pp.8-13, (2017).    DOI
9 S. J. Moon and M. H. Yoon. "Plant growth promotion effect of Arthrobacter enclensis Yangsong-1 isolated from a button mushroom bed", Journal of Mushroom. Vol.17, No.1, pp.12-18, (2019).    DOI
10 N. Napawit P. Watanalai, S. Wisuwat, I. Bungonsiri. "Plant growth-promoting properties of Streptomyces spp. isolates and their impact on mung bean plantlets' rhizosphere microbiome", Frontiers in microbiology. Vol.13, pp.247-252, (2022). 
11 J. C. Lee and K. S. Whang. "Optimization of Indole-3-acetic Acid (IAA) Production by Bacillus megaterium BM5", Korean Journal of Soil Science and Fertilizer, Vol.49, No.5. pp.461-468, (2016).    DOI
12 B. Schwyn, and J. B. Neiland. "Universal chemiacl assay for the detection and determination of siderophores", Anal Biochem. Vol.160, No.1, pp.47-56, (1987).    DOI
13 E. A. Rodriguez Caeres. "Improved medium for isolation of Azospirillum spp", Appl Environ Microbiol. Vol.44, No.4, pp.990-991. (1982).    DOI
14 O. S. Kim, Y. J. Cho, K. Lee, S. H. Yoon, M. Kim, et al. "Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species", Microbiology. Vol.62, pp.716-721, (2012). 
15 K. Tamura, G. Stecher, D. Peterson, A. Filipski, and S. Kumar. "MEGA6: Molecular evolutionary genetics analysis version 6.0", Molecular Biology and Evolution. Vol.30, No.12, pp.2725-2729, (2013).    DOI
16 K. S. Choi, S. K. Song, D. E. Koo, H. N. Lee, H. I. Sung, J. J. Kim. "Characteristics of Seed and Germination of Rhododendron mucronulatum by Collection Dates and Germination Temperatures", Journal of Korean Society of Forest Science. Vol.107, No.3, pp.237-244, (2018).   DOI
17 H. S. Lim, J.M. Lee, S. D. Kim. "A plant growth promoting Pseudomonas fluorescens GL20: Mechanism for disease suppression, outer membrane receptor for ferric siderophore, and genetic improvement for incresed biocontrol efficacy", Journal of Microbiology and Biotechnology. Vol.12, No.2, pp.249-257, (2002).