• Proceedings of the Ginseng society Conference
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• 1974.09a
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• pp.101-113
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• 1974

The radioactive compound sodium $acetate-U-C^{14}$ (C-14 acetate) was administered to two- and four-year-old July and September American ginseng (Panax quinquefolium L.) plants and cuttings. The C-14 acetate uptake was approximately $99\%.$ The autoradiochromatograms suggest that the saponins(panaquilins) isolated by preparative thin-layer chromatography contained impurities, especially those isolated from the leaf and stem extracts. The root and fruit methanol extracts yielded relatively pure saponins. The large amounts of panaquilin B and its proximity to panaquilin C on preparative thin-layer plates resulted in some admixing. The average concentration $(\%$ plant dry weight) of semipurified saponins were high in the leaves $(13.8\%),$ compared to fruits $(9.8\%),\;stems\;(7.9\%)\;and\;roots\;(6.3\%).$ The average percentage of C-14 acetate incorporation into panaquilins was $4.8\%.$ The average percentage of C-14 acetate incorporation into panaquilins B and C was higher $(1.40\%\;and\;1.13\%,$ respectively) than that into panaquilin C, (d), G-1 and G-2 $(0.75\%,\;0.65\%,\;0.13\%\;and\;0.53\%,$ respectively). Panaquilin synthesis may be depending upon the part collection period and age of the plant. The average percentage of C-14 acetate incorporation into panaquilin B is high in roots $(0.58\%)\;and\;stems\;(0.48\%);$ that into panaquilins C and (d) high in leaves $(0.40\%\;and\;0.45\%,$ respectively); and that into panaquilin E high in roots and leaves $(0.55\%\and\;0.50\%,$ respectively). Panaquilin G-2 was synthesized in all parts of plants. The panaquilins appear to be biosynthesized more actively in July than September (exception-panaquilin G-l). Panaquilins B, C and G-1 may be biosynthesized more actively in four-year-old plants and panaquilins (d) and E more actively in two-year-old plants. The results from expectance with cuttings suggest that the panaquilins are synthesized de novo in the above-ground parts of ginseng plants, and that panaquilin G-l may be synthesized de novo in the leaf. It is known from the tissue culture studies that panaquilins are produced by leaf, stem and root callus tissues and callus-root cultures of American and Korean ginseng plants. Panaquilins may actively be synthesized de novo in most any cell or organ of the ginseng plants. It was verified that C-14 acetate was incorporated into the panaxadiol portions of the panaquilins of two-year-old plants (sp. act., 0.56 $m{\mu}Ci/mg$) and four-year-old plants (sp. act., 0.54 $m{\mu}Ci/mg$).

• Korean Journal of Medicinal Crop Science
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• v.5 no.3
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• pp.202-205
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• 1997

This study was carried out to determine the effect of sowing time on the flowering, growth and yield of Scutellaria baicalensis Georg, which was collected from Yeochon district, cultivated after barley in the southern coastal areas of Korea. Emergence and flowering dates in the sowing time of June 1 were earlier than those of the other sowing times. In the sowing time of June 1, length and diameter of main stem, number of node per main stem, number of branch per plant and dry weight of stem leaves were greater than those of sowing times of June 10 and June 20. Yield components such as main stem length and diameter, main stem numbers, branches per plant, dry weight of stem leaves, main root length and thickness, number of large root and fine root per plant, and dry weight of root were the highest at the sowing time of June I as the yield of 71.3kg/10a. Optimum sowing time of Scutellaria baicalensis Georg cultivated after barley was June 1 in southern areas of korea.

• Journal of Environmental Science International
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• v.5 no.6
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• pp.813-826
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• 1996

The toxic effects of aluminium (Al) on growth, chlorophyll content, $\delta-aminolevulinic$ acid dehydratase (ALAD) activity and anatomy of root and shoot were investigated in 7-day-old azuki bean (Vigna angularis) seedlings. Significant depressions in root elongation was observed in the low concentrations of Al (50, 100 $\muM)$ and increasing Al concentrations caused a sharp decline of root and shoot growth. The degree of inhibition was dependent upon Al supply. Exposure to 50 $\muM$ Al or more inhibited root elongation within 1 day. In the 50 $\muM$ Al treatments, a recovery of root growth was seen after 7 days exposure. In contrast, lateral root initials was little affected by Al exposure. Al toxicity symptoms and growth responses were more well developed in the roots than in the shoots. Analysis of Al localization in root cells by hematoxylin stAlning showed that Al entered root apices and accumulated in the epidermal and cortical cells immeadiately below the epidermis. There was a good positive correlation between the level of chlorophyll and ALAD activity. Increasing Al concentrations caused a decrease in total chlorophyll contents, accompanied by proportional changes in ALAD activity, suggesting a cootr-dinated reduction of a photosynthetic machinery. Al exerted specific influence on the morphology of root ann shoot. At higher concentrations of Al the roots induced drastic anatomical changes. The epidermal cells were disorganized or destructed while the cortical cells exhibited distortion of cell shape and/or disintegration. The diameter of root and transectional area of cortical cells decreased considerably with Al treatment. In the shoot Al also enhanced reduction of diameter of shoot and cell size. Gross anatomy of leaves treated with Al did not differ significantly from the controls, except for fewer and smaller chloroplast. Our results indicate that toxic effect of Al appear to be manifested primarily in roots and secondarily on shoots, and changes in root morphology are related to changes in the root growth patterns. Results are further discussed in re181ion to the findings in other plant species, and it is concluded that Al causes morphological, structural and, presumably, functional damage to the roots of the species investigated.

• Korean Journal of Medicinal Crop Science
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• v.21 no.4
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• pp.296-300
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• 2013

This study was carried out to have the basic and applied information to develop the cultivation methods and models and to increase the productivity of high-quality ginseng. Plant height was dramatically increased from one year old to four years old, and then it was grown up very slowly. Stem length was shown similar tendency as the plant height did. Its range were from 9.4 cm in one year old to about 42.4 cm in four year-old ginseng. The leaf area was clearly increased until four years old, $10.1cm^2$ in one year old to $204.9cm^2$ in four years old, while it was slightly increased after four years old. Root length was continuously increased from one year old until four years old, and then it was grown up very slowly. Tap root length which was measured was appeared about 8 cm from three years old to six years old, showing not much different among the year-olds. The root diameter was continuously increased from one year old to six year-old ginseng, about 2.9 mm and about 19 mm, respectively. The moisture content was higher on the shoot than that on the root. It was slightly decreased as they were getting old. In shoot part, the rate of leaves and stems based on dry weight was about 1 : 1. In the root, the dry weight of tap root was heavier than that of lateral root. The dry weight of shoot was decreased after four years old, while it of the root was continuously increased until six years old, resulting the production of ginseng root. Based on the results of this study, growth characteristics of shoot and root of ginseng were dramatically increased from one year old to four years old and then their growths were appeared different between shoot and root, showing not much growing in shoot and keep growing in root.

• Journal of Ginseng Research
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• v.36 no.4
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• pp.430-441
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• 2012

This study was conducted to evaluate the effects of natural bioactive products such as Manda enzyme (T1), Yangmyeongwon (T2), effective microorganisms (T3), and Kelpak (T4) on the growth and ginsenoside contents of Panax ginseng cultured in an aeroponic system using a two-layer vertical type of nutrient bath under natural light conditions. The growth of ginseng plants showed specific characteristics according to the positions in which they were cultured due to the difference of light transmittance and temperature in the upper and lower layers during aeroponic culture in a two-layer vertical type of system. The growth of the aerial part of the leaves and stems of ginseng plants cultured in the lower layer (4,000 to 6,000 lx, $23^{\circ}C$ to $26^{\circ}C$) of the nutrient bath was observed to be superior to that of the ginseng plants cultured in the upper layer (12,000 to 15,000 lx, $25^{\circ}C$ to $28^{\circ}C$). The leaf area was significantly larger in the treatment of T2 and T4 (46.70 $cm^2$) than with other treatments. Conversely, the values of the root weight and root diameter were higher in ginseng plants cultured in the upper layer of the nutrient bath. The root weight was significantly heavier in the treatment of T4 (6.46 g) and T3 (6.26 g) than with other treatments. The total ginsenoside content in the leaves and roots was highest in the ginseng plants cultured by the treatment of T1, at 16.20%, while the total ginsenoside content obtained by other treatments decreased in the order of T4, T5 (control), T2, and T3, at 13.21%, 12.30%, 14.84%, and 14.86%, respectively. The total ginsenoside content of the ginseng leaves was found to be significantly higher in the treatment of T1 in the lower layer of the nutrient bath, at 15.30%, while the content of the ginseng roots in the treatments of T3 and T4, at 1.27% and 1.23%, respectively, was significantly higher than in other treatments in the upper layer of the nutrient bath.

• Korean Journal of Soil Science and Fertilizer
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• v.3 no.1
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• pp.11-16
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• 1970

In application of manganese and silica to Akiochi soil, growth of rice plant with potassium was remarkably increased, but the growth without potassium was stunted and the number of the tiller was relatively reduced in comparison with that receiving potassium. The minus potassium plant developed tiny brown spots on the leaves and color of the leaves was dark green. They had smaller leaves and shorter stem while heading advanced. Roots with potassium were long and thick, and new ones developed, while roots without K were very poor, the branch and hair roots very thin, and severe root rot was observed. With minus potassium treatment, the root rot of Jin Heung(commercial variety) was more severe than Nonglim 6(Akiochi resistant). Growth of plant without potassium was very poor, and potassium content of the plant was very low, while nitrogen, phosphorus, silica, iron and manganese contents were higher than potassium supplied plant. Application of $Mn+SiO_2$ to Akiochi soil lowered iron content of plant, and iron content of the plant were also reduced by application of potassium. From the results obtained it can be concluded that root damage of minus potassium plant grown in Akiochi soil supplied $Mn+SiO_2$ was caused by potassium deficiency. Potassium, and $Mn+SiO_2$ applications lowered iron content in the plant grown on Akiochi soil.

• Journal of Korea Foresty Energy
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• v.21 no.3
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• pp.46-53
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• 2002

The study was carried out to determine the growth and biomass production of deciduous trees including Betula pendula, Fraxinus rhynchophylla, Cornus controversa subjected to artificial shade treatment of three levels in nursery field. The results were as follows; The height growth of seedlings was no difference subjected to artificial shade treatment. The root collar diameters of the relative growth rates of seedlings grown in full sun showed 29-32% as compared with those subjected to the shade treatment of 7-10% light intensities of full sun. Corrus controversa of the root collar diameters of the relative growth rates were better in the seedlings grown in 26-34% light intensities of full sun. Total dry mass including the dry mass of leaves, shoot and root were as a whole decreased with shade treatment. The ratio of the dry mass of leaves and stem increased the dry mass of root. T/R ratio of the seedlings increased by decreasing the relative light intensity. And the T/R ratio of 7-10% light intensities of full sun was ranged from 1.00~2.27 were greater in the full sun light was ranged from 0.51~l.13. Light intensity by artificial shade treatment decreased in deciduous trees when compared on the whole, it showed tendency that SLA increases, increased that seeing resemblant tendency in LAR and LWR and changed of light intensity is strong, it increased that showed difference as statistical.

• Journal of Sericultural and Entomological Science
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• v.41 no.1
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• pp.29-35
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• 1999

This experiment was carried out to investigate the effect of silkworm powder, mulberry leaves and mulberry root bark administered to S.D. rat on gastrointestinal function. Plot containing ten percent sericultural products(silkworm powder, mulberry leaves and mulberry root bark) was supplied with S.D. rat. The food efficiency ratio(FER) of S.D. rat fed with sericultural products were 0.1486~0.1573. The transit time also were 512.5~574.0 minutes, and transit speed were 25.05~29.11 mm/min. The pH of S.D. rat's feces fed with sericultural products were 5.95~6.72. The daily amount of S.D. rat's feces were 4.12~5.42g. As above results, sericultural products was evaluated to improve the function of S.D. rat's gastrointestine.

• Food Science of Animal Resources
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• v.23 no.1
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• pp.46-49
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• 2003

Classwort (Salicornia herbacea L.), a halophyte, is a potential functional food resource in Korea. This study was conducted to determine the antioxidant activity of glasswort, as a functional food material, on the lipid oxidation of pork. To compare antioxidant effect of different parts of glasswort, samples such as dried ground leaves, stems, and roots of glasswort were prepared. The antioxidant activity was determined by the TBARS(Thiobarbituric Acid Reactive Substances) method. Freeze-dried leaves of glasswort at 0 day of storage had the lowest TBARS values, suggesting the highest antioxidant effect. But the antioxidative effect of freeze-dried leaves of glasswort was less than that of BHT. At 7 days after storage, however, the values were not different from oven-dried leaves. The oven-dried leaves at 14 days after storing should highest antioxidative activity. In conclusion, antioxidative effect of glasswort was apparently exhibited through measurement of TBARS. Antioxidative effect from ground leaf sample of glasswort harvested in coastal region was the highest, followed by root and stem samples. Glasswort had twice as high antioxidative effect as sea salt and bamboo salt.

• Journal of the Korea Organic Resources Recycling Association
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• v.2 no.1
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• pp.65-73
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• 1994

Earthworm cast was investigated in this study in order to domestically develop inexpensive and safe plug nursery soil. 20,30,40 and 50% of earthworm cast were added to the nursery soil as vegetable plant growth media, in which peatmoss and vermiculite constituted the rest of the soil. The effects of earthworm cast on the growth of pepper (Capsicum annuum L.) seedling were obtained as follows. 1. According to the growth stages, significant differences(p=0.001) were recorded in number of leaves, leaf area and biological dry matter yield per plant(shoor or root dry matter yield per plant). In view of mixing ratios in the nursery soil, number of leaves, leaf area and stem weight per plant showed differences significantly at 1% level and leaf weight, root weight and biological dry weight per plant at 5% level, respectively. 2. The nursery soil with earthworm cast showed increased number of leaves and leaf area per plant compared to the commercial nursery soil particularly in the later stage of this study than in the earlier stage 3. Through the total period of seedling growth, leaf weight, stem weight and root weight per plant in the nursery soil with earthworm cast were genrally higher than those in the control and this trend was apparant in the treatment of more than 40% of mixing ratio with earthworm cast. 4. Although leaf weight per plant was higher than stem weight per plant till the 3rd week, from the 4th week stem weight per plant was getting higher. In the later stage of seedling growth, the stem weight was higher in the earthworm cast mixed treatment than that in the control. 5. There was no significant difference on biological dry matter yield in the earlier stage of this study, however in the later stage, it was higher in the earthworm cast mixed treatment than that in the control.