• Journal of Bio-Environment Control
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• v.6 no.1
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• pp.1-14
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• 1997

This experiment was conducted to find out the compositions of nutrient solution for closed system in substrate culture of cucumber. Cucumber(Cucumis sativus L. cv. Eunsung baekdadagi) plants were grown in the substrates supplied with the nutrient solutions whose strengths were 1/2, 1, and 3/2 of the original concentration developed by National Horticultural Research Station in Japan. By increasing the nutrient concentrations, plant height decreased but leaf length, leaf width, and leaf number showed little differences. A number of marketable fruit and marketable yield were the highest in the concentration of 1 strength. The nutrient compositions of solution developed for closed system in cucumber substrate culture were N 11.4, P 3.3, K 6.0, Ca 4.5, and Mg 3.5 me.$\ell$$^{-1}$ during the vegetative growth period and N 10.4, P 3.3, K 5.0, Ca 4.5, and Mg 3,5 me.$\ell$$^{-1}$ during the reproductive growth period. To examine the suitability of nutrient solution developed in the above experiment, cucumber plants were grown in the substrates supplied with different solutions and concentrations - Yamasaki's nutrient solution(Yamasaki) of 1 S, nutrient solution of Research Station for Greenhouse Vegetable and Floriculture on the Netherlands(PTG) of 1 S, nutrient solution developed in the above experiment(SCU) of 1/2, 1, and 3/2 S. EC and pH in root zone changed little in the all treatments during growing period. As cucumber plants grew, the concentrations of N, P, and K in root zone decreased but Ca concentration increased. Net $CO_2$ assimilation rate of cucumber leaves was high in SCU of 1 and 3/2 S, and Yamasaki of 1 S. Growth of cucumber plants was the lowest in SCU of 1/2 S.

• Journal of Life Science
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• v.25 no.10
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• pp.1091-1097
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• 2015

We have isolated and characterized an ascorbate peroxidase (APx) gene, OsAPx1 from rice. Northern and Western blot analyses indicated that at young seedling stage, OsAPx1 mRNA was expressed highly in root, shoot apical meristem (SAM) and leaf sheath than leaf. In mature plant, OsAPx1 gene expressed highly in root, stem and flower but weakly in leaf. OsAPx1 gene and protein expression level was induced in leaves inoculated with Magnaporthe oryzae (M. oryzae) and Xanthomonas oryzae pv. oryzae (Xoo). Phytohormones treatment showed that OsAPx1 was up-regulated by jasmonic acid (JA), but was down regulated by ABA and SA co-treatments with JA, resulting that they have antagonistic effect on pathogen responsive OsAPx1 expression. Phylogenetic analysis illustrated that Arabidopsis AtAPx1 has a close relationship with OsAPx1. In AtAPx1 knock out lines, the accumulation of O₂^- and H₂O₂ are all highly detected than wild type, revealing that the high concentration of exogenous H₂O₂ cause the intercellular superoxide anion and hydrogen peroxide accumulation in AtAPx1 knockout plant. These results suggested that OsAPx1 gene may be associated with the pathogen defense cascades as the mediator for balancing redox state by acting ROS scavenger and is associated with response to the pathogen defense via Jasmonic acid signaling pathway.

• The Korean Journal of Ecology
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• v.4 no.3_4
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• pp.93-108
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• 1981

This investigation includes the sick soil phenomenon caused by the self-poisoning of Setaria italica, Sorghum nervosum, Zea mays and Miscanthus sinensis among Poaceae. It elucidates whether the poison is directly excreted from the root or the secondary product resulting from the decomposition in the soil; the effect of Miscantus sinensis on the germination and growth of other plants, and the effect of Zea mays grown between furrows to shade Angelica gigas on its growth. The results obtained are as follows; Supplied with the leakage water from the pots, in which the same plants as the test ones were grown, in anticipation of the poison to be directly excreted from their roots, Sataria itlaica and Zea mays exhibited the growth inhibition more than 30%, whereas Sorghum nervosum and Miscanthus sisnensis were not effected in growth at all. When cultivated in the soils mixed with the roots of the some plants as the test ones, in anticipation of the poison to be the secondary product resulting from the decomposition in soil, Setaria italica and Zea mays showd growth inhibition of more than 50%, which is greater than that of the case of the leakage water, and Miscanthus sinensis exhibited no inhibition either, whereas Sorghum nervosum in the 50% plot showed heavy growth inhibition of more than 80% to the case of the leakage water. The common or uncommon plants found easily in the group of Misscanthus scinenis were not affected by the extracts of the steam and leaves of Miscanthus sinensis in germination and growth. Supplied with the leakage water from the pots in which Miscanthus sinensis was grown, among Lespedeza crytobotrya, Oenothera odorata, Raphanus sativus val'. acarlthiformis, Zoysia japonica, Patrinia scabiosaefolia. which are easily found in the group of Miscanthus sinensis, only Patrinia scabiosaefolia was slightly inhibited in growth in the 100% plot, whereas the others did not show any inhibition at all. Mean while, Amaranthus patulus. Solanum nigrum, Capsella bursa-pastoris val'. triangularis, Alopecurus amurensis, Chenopodium album val'. centrorubrum, which could not be found in the group of Miscanthus sinensis, were all distinctly inhibited. In the experiment on the effect of Zea mays on the growth of Angelica gigas, its growth was severely inhibited by one-half to two thirds with the increased concentration in both the cases of growing in the mixture of the soil and the powdered root of Zea mays and being supplied with the leakage water from the pot in which Zea mays was grown.

• Journal of Korean Society of Forest Science
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• v.99 no.6
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• pp.922-928
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• 2010

There is a little information on the effect of calcium cloride ($CaCl_2$) which is used as deicing salt in Korea on the physiological responses of the street trees. Prunus sargentii is one of the most widespread tree species of street vegetation in Korea. In this study, the effect of $CaCl_2$ on photosynthetic apparatus such as chlorophyll fluorescence image and light response curve of P. sargentii in relation to their leaf and root collar growth responses were investigated. To study the effect of $CaCl_2$ treatment in the early spring, we irrigated twice in rhizosphere of P. sargentii (3-year-old) planted plastic pots with solution of 0.5%, 1.0%, 3.0% $CaCl_2$ concentration before leaf expansion. Results after treatments, total chlorophyll contents and the chlorophyll a/b, photosynthetic rate, quantum yield, dark respiration decreased with increasing $CaCl_2$ concentration. On the contrary, light compensation point increased with increasing $CaCl_2$ concentration. Through the linear regressions of correlation of photosynthetic rate with photosynthetic parameters (quantum yield, dark respiration and light compensation point), we found a significant relationship (p<0.05) between photosynthetic rate and quantum yield and light compensation point except dark respiration. Calcium cloride ($CaCl_2$) induced inhibition of photochemical efficiency ($F_v/F_M$) and non-photochemical quenching (NPQ) were found in treatments of $CaCl_2$, and these reduction rates between control and CaCl2 treatments were drastically showed at 80 days. We suggest that physiological activities are limited from treatment of $CaCl_2$. These reductions of photosynthetic apparatus ability caused eventually the reduction of leaf and diameter at root collar growth.

• The Korean Journal of Ecology
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• v.24 no.3
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• pp.157-168
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• 2001

Herbicides have been used to control weeds for decades. If detoxification upon exposure to herbicides requires considerable amounts of energy, it could affect the pattern of resource allocation to growth and reproduction of crops. We examined the effects of three levels of a herbicide (Control, Low, and High) on germination, growth and reproductive characters of Glycine max treated twice, i.e., before and after seed germination. Since flowering time of G. max was separated into two groups, flowering time was also considered as a variable in this study. The rate of seed germination tended to be higher at the low level of herbicide compared to other levels. Chlorosis and shape variation of leaves were apparent after the second herbicide treatment, but completely disappeared after six weeks of treatment. The herbicide effects on growth characters were somewhat different between early and late flowering plants, but plants treated with both low and high levels of herbicide reduced their growth compared to those in the control group regardless of flowering time. Plants at the high level of herbicide exhibited the highest growth rate later in the season, suggesting that plants compensated to some extent for reduced growth. However, growth reduction among plants at the high level of herbicide was persistent until the end of growing season. Among plants flowered late in the season, plants in the control level bore a higher number of nodules per plant than those in other levels; such a pattern did not exist among plants flowered early in the season. Plants treated with low and high levels of herbicide produced a lower number of flowers than those in the control. Thus, the herbicide examined affected not only the growth and reproductive characters of non-target crops but also the development and growth of root nodules.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.1
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• pp.50-56
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• 2011

This study was designed to investigate variation of pinitol content in different parts of seventeen legume species including silk tree (Albizia julibrissin). D-pinitol has been demonstrated to exert insulin-like and anti-inflammatory effects. These legumes were collected from Gyeongsangbuk-Do in Korea. Significant difference in pinitol content was observed among 17 different legume species. However, it was the highest in sericea lespedeza (Lespedeza cuneata). The highest pinitol content was observed in leaf followed by stem, pod shell, seed and root among plant parts. Legume plants which had higher pinitol content in leaves were chinese pea shrub, bastard indigo, wild cowpea and sericea lespedeza, having 59.9 mg/g, 62.2 mg/g, 69.6 mg/g and 65.4 mg/g, respectively. Stem of astragali radix showed the highest pinitol content among all leagumes, which was 34.0 mg/g. In case of root, kudzuvine showed the highest pinitol content followed by licorice and chinese pea shrub, which were 24.6 mg/g, 16.9 mg/g, and 16.5 mg/g, respectively. However, in the case of pod shell and seed, only lablab purpureus and sericea lespedeza showed the highest pinitol content 52.5 mg/g (for pod shell) and 24.9 mg/g (for seed), respectively. Concluding, the sericea lespedeza showed the highest total pinitol content of whole plant followed by chinese redbud, chinese pea shrub, bastard indigo and silk tree. Considering the pinitol content and harvesting yield, sericea lespedeza can be used as a practical medicinal herb.

• Journal of Soil and Groundwater Environment
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• v.13 no.4
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• pp.30-39
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• 2008

The uranium removal efficiency of rhizofiltration was investigated in lab scale experiment. Three plants such as sunflower (Helianthus annuus L.), bean (Phaseolus vulgaris var.), and Indian mustard (Brassica juncea (L.) Czern.) were cultivated in artificially contaminated solution by uranium at 30 ${\mu}g$/L and 80 ${\mu}g$/L for 72 hours. The removal efficiencies of three cultivars were calculated from the ICP/MS analysis of uranium mass in solution and the plant. For Helianthus annuus L., more than 80% of initial uranium were removed from the solution and the uranium concentration of residual solution maintained lower than 10 ${\mu}g$/L. For Phaseolus vulgaris var. and Brassica juncea (L.) Czern., their uranium removal efficiencies ranged from 60 to 80%. Even the uranium concentration of solution was higher than 500 ${\mu}g$/L, these cultivars removed more than 70% of initial uranium by rhizofiltration, suggesting that the rhizofiltration has a great capability to remove uranium in the contaminated groundwater. The removal efficiency of rhizofiltration by using Brassica juncea (L.) Czern decreased from 83% to 42% with the increase of pH in solution. From the results of the analysis for the uranium accumulation in plants, 99% of uranium transferred into the plant from solution were accumulated in the root and only 1% of uranium existed in the shoot part (including leaves), suggesting that the cost and the time to treat massive grown plants after the rhizofiltration could be dramatically cut down because only their root parts needs to be treated. Finally, the genuine groundwater having high uranium concentration (81.4 ${\mu}g$/L), sampled from Daejoun area, was used in the experiment. The uranium removal efficiency of Helianthus annuus L. for the real groundwater was higher than 95%, investigating that the rhizofiltration is the very useful method to remediate uranium contaminated groundwater.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.2
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• pp.140-148
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• 2018

As the global warming causing desertification increase, there is growing concern about damage of crops. It was to investigate how the treatment with hydrogen peroxide before leaf development affects the growth and yield of sorghum for minimizing a damage of crops to drought. The germination experiment was conducted at alternating temperature of $25^{\circ}C/20^{\circ}C$(12 hr/12 hr) under water stress condition of 0 ~ -0.20 MPa adjusted with PEG solution containing 0 and 10 mM $H_2O_2$. In order to know the effect of foliar application of hydrogen peroxide on the growth of sorghum, 10 mM hydrogen peroxide was treated to leaves at 3-leaf stage of sorghum growing in greenhouse conditions. Seed germination rate was increased by 20% in hydrogen peroxide treatment as compared to the Control. under water stress conditions (-0.15 ~ -0.20 MPa). The length of seedlings was also on the rise by the hydrogen peroxide treatment. In the greenhouse pot experiment, the morphological characteristics (plant height, stem diameter, leaf length, and leaf number) and physiological characteristics (chlorophyll content, chlorophyll fluorescence (Fv/Fm), stomatal conductance) were higher in the plants treated with hydrogen peroxide under the drought stress condition than those of plants of $H_2O$ treatment. Experiment conducted with the soil moisture gradient system showed that the foliar application of hydrogen peroxide increased photosynthetic ability of sorghum plant with respect to SPAD value and stomatal conductance and rooting capacity (root weight and root length) under drought condition. Generally, hydrogen peroxide treatment in sorghum increased the tolerance to drought stress and maintained better growth due to ameliorating oxidative stress.

• Journal of the Korean Institute of Landscape Architecture
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• v.47 no.4
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• pp.61-67
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• 2019

The purpose of this research is to evaluate the salt tolerance of the Miscanthus sinensis and to characterize the content of pigments in the leachate in relation to calcium chloride ($CaCl_2$) deicing salt. Miscanthus sinensis were cultured at five different concentrations of calcium chloride deicing salt, 0, 1, 2, 5, and $10g{\cdot}L^{-1}$ (referred to Cont. C1, C2, C5, and C10) for four months. The salt tolerance and leachate while growing Miscanthus sinensis on soil which was artificially contaminated by calcium chloride deicing salt. Soil chemical properties (pH, E.C., $Ca^{2+}$, $Na^+$, $K^+$, and $Mg^{2+}$) and plant growth parameters (plant height, leaf length, leaf width, number of leaves, shoot fresh weight, root fresh weight, shoot dry weight, an root dry weight) were evaluated. Soil pH decreased, while electrical conductivity significantly decreased ($p{\leq}0.05$) with a higher concentration of deicing salt $0g{\cdot}L^{-1}$ (Cont.). The increase in the concentration of chloride-based exchangeable cations, along with the increase in the deicing salt treatments, were observed in $Ca^{2+}$ > $Na^+$ > $K^+$ > $Mg^{2+}$. Notably the $Ca^{2+}$ exchangeable cations were 83~90% higher than the others. The growth of Miscanthus sinensis significantly increased ($p{\leq}0.05$) with the concentration of deicing salt higher than $1g{\cdot}L^{-1}$ (C1) when compared to 0 g/L (Cont.), except for the $10g{\cdot}L^{-1}$ (C10) treatment. The results determined that the contamination of soil by deicing salt could negatively impact the soil and Miscanthus sinensis was a tolerant species for the deicing salts. Further research will be focused on soil improvement additives and the stable stimulated plant growth of Miscanthus sinensis and a formulation on that basis for the soil-plant continuum.

• Journal of Bio-Environment Control
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• v.27 no.1
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• pp.86-93
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• 2018

This present study was conducted to develop the domestic cultivation kit using water celery(Oenanthe Stolonifera DC.) seed. As the result of germination rates in 3 type inbred lines, the IT 232354 had the highest initial germination rate and final germination rate, and was selected as the inbred line to be used in the cultivation kit. The development of the domestic cultivation kit was carried out using the IT 232354 seeds. It was possible to cultivate up to 3 times harvest using the same root in this cultivation kit, though the growth decreased rapidly in the $4^{th}$ cultivation. As a result of investigating the effects of the type of nutrient solution on growth of water celery, the overall growth was the lowest in the nutrient solution for Oenanthe Stolonifera DC.(N.S.D.). The shoot growth was similar to that of nutrient solution for leaves and stem vegetables (N.S.L.S.) and amino acid fermentation by-product liquid (A.F.B.L.), however in the root growth, the N.S.L.S. was more effective than A.F.B.L. When it was harvested 4 times consecutively after 1 time of planting, the last survival ratio of A.F.B.L. was 100% while their ratios were 96.4% and 49.8% each for N.S.L.S. and N.S.D. For the growth by cultivation kit type, the hole type cultivation kit increased slightly compared to the 3 hole type cultivation kit in the $1^{st}$ and $2^{nd}$ harvest, but there was no difference in the $3^{rd}$ and $4^{th}$ harvest. Total yield of one cultivation kits showed the 3 hole type cultivation kit is much higher than the 2 hole type cultivation kit. According to the results of this experiment, it is possible to harvest three times by planting one times if it was cultivated using N.S.L.S. and A.F.B.L. in the 3 hole type cultivation kit.