• Title/Summary/Keyword: room strain

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Quality Characteristics of Mungbean Starch Gels with Various Hydrocolloids (친수성 다당류 첨가가 녹두전분 겔의 품질 특성에 미치는 영향)

  • Choi, Eun-Jung;Oh, Myung-Suk
    • Journal of the Korean Society of Food Culture
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    • v.24 no.5
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    • pp.540-551
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    • 2009
  • This study was conducted to investigate the quality characteristics of mungbean starch gels containing various hydrocolloids (carrageenan, locust bean gum and xanthan gum) during room temperature storage ($25^{\circ}C$ for 24, 48 and 72 hours). Carrageenan and xanthan gum reduced the pasting viscosity of mungbean starch, whereas the locust bean gum increased the viscosity. The melting characteristics, as assessed by DSC, showed that carrageenan and xanthan gum delayed gelatinization of mungbean starch and the locust bean gum had no effect on this property. The lightness (L) of the gels with the locust bean gum was similar to that without the additive during storage, whereas that with carrageenan and xanthan gum was higher than that without the additive. Hardness, chewiness and gumminess of the gels with the locust bean gum was higher than that without the additive during storage, whereas that with carrageenan and xanthan gum was lower than that without the additive. The rupture stress, rupture strain and rupture energy of the gels with carrageenan and xanthan gum was lower than that without the additive during storage, whereas that with the locust bean gum was similar to that without the additive. In the sensory evaluation, springiness and cohesiveness of the gels with carrageenan and xanthan gum were lower than those without the additive, whereas springiness, brittleness and hardness of the gels with the locust bean gum were higher than those without the additive. In addition, the overall acceptability of the gels with the locust bean gum improved. The above results showed that carrageenan and xanthan gum lowered the quality characteristics of the mungbean starch gel and the locust bean gum improved them. Thus, the addition of 0.5% locust bean gum is an appropriate method for improving the quality characteristics of mungbean starch gel.

Fabrication and mdchanical properties of $AC4A/SiC_p$ composites by mechanical alloying (기계적합금화에 의한 $AC4A/SiC_p$복합재료의 제조 및 기계적 특성)

  • Lee, Byung-Hun;Cho, Hyung-Jun;Lim, Young-Ho;Lee, Jun-Hee
    • Korean Journal of Materials Research
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    • v.4 no.6
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    • pp.651-661
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    • 1994
  • Utilizing Mechanical Alloy Process, that were obt,ained the results from investigated formation process of AC4A/$SiC_p$. composite material powders and mechanical properties of their extrusion materials. The obtained results are as follow conclusions. AC4A-lOwt.% $SiC_p$ powders which were mechanically alloyed at 150rpm for 420min have been obtained finely and uniformly rounded powder particals that were reached the steady state which was saturated micro hardness about tlv 230 in the range size of 1 0 ~ 2 0$\mu \textrm{m}$. EDAX analysis tests have been resulted in a little amount of I'e conrents increasing with MA times, the artifical aging of AC4A/S$SiC_p$ composite materials was obtained the hardness with solution treated at $525^{\circ}C$ for lOhrs the maximum value of Hv 230 with aging at. $170^{\circ}C$ for 1000min. The Intensity and width of X-ray diffraction pattern were decreasing and widening because of grain boundary refinement and heterogeneous strain during mechanical alloying. Tensile tests at room temperature were carried out the maximum value of 37 Kgf/$\mu \textrm{mm}^2$ with ext,rused materials, 27 Kgf/$\mu \textrm{mm}^2$ with heat treated them at $500^{\circ}C$.

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Studies on the induction of pregnancy and the number of fetuses during pregnancy in rats

  • Choi, Seung-Hee;Cho, Yong-Seong;Kim, Min-Ji;Lee, Chae-Hyeok;Seong, Hwan-Hoo;Baek, Soon-Hwa;Lee, Jang-Hee
    • Journal of Animal Reproduction and Biotechnology
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    • v.35 no.3
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    • pp.232-238
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    • 2020
  • This study used adult wistar-based rats to observe the sexual cycle as a morphological characteristic of vaginal epithelial cells by vaginal smearing, and investigated the fetal number through mating with male rats of the same strain. The target animal was a 12 to 13-week-old Wistar-based mature unlighted rat (weight 220 g to 240 g), room temperature 23 ± 2℃, 14 hours artificial lighting (05:00 to 19:00 hours), 10 hours Adapted individuals were used for rearing for at least 2 weeks under the conditions of the darkroom (19:00 to 05:00). The feed was managed for free feeding of pellet feed for animals and water. The vaginal smearing method was used for the experiments by observing the sexual cycle every morning and confirming that the normal sexual cycle of 4 or 5 days was repeated at least 2 cycles or more. As a result, the proestrus was found to have few red blood cells, the cells and nuclei were rather large and round, and many nucleated cells were identified. In the case of the estrus, the cells were large and the nuclei were not stained, and most of the keratinocytes were found. In addition, in the metestrus and diestrus, there were many white blood cells, and it was confirmed that nucleated epithelial cells and keratinocytes were significantly reduced. The pregnancy period was 21 ± 1.8 days, and the number of live births per delivery was 11.9 on average. The number of fetuses on the 8th and 10th days of pregnancy were 15.2 ± 0.4 and 15.4 ± 0.3, respectively. On the contrary, the number of fetuses on the 12th day of pregnancy was 12.9 ± 0.6, which was significantly (p < 0.05) decreased compared to the 10th day of pregnancy, and the number of fetuses was similar until delivery. As a result of investigating the change of body weight according to the birth weight and growth stage after delivery, the birth weight of female and male was 9.2 ± 2.0 g and 9.8 ± 2.5 g, respectively. After that, until the 16th day, the female and the male showed similarly moderate weight gain, and then showed a rapid weight gain until the 21st day of lactation. With reference to the results of this study, it is expected to be used as basic data for determining the mating time of rodents and controlling pregnancy and fetal number.

Studies on Cryopreservation of 8-Cell Mouse Embryos (생쥐 8세포기 수정란의 동결보존에 관한 연구)

  • 최미후;이규승;박칭식;서길웅
    • Korean Journal of Animal Reproduction
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    • v.11 no.3
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    • pp.155-160
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    • 1987
  • This study was carried out to determine the effects of cryoprotectants, freezing and thawing rates on the survival of 8-cell mouse embryos. The female ICR mice were induced to superovulated by intraperitoneal injections of 5 i.u. PMSG and 5 i.u. HCG given 48h apart and then were paired with males of the same strain. They were killed and embryos were flushed from the oviducts and uteri on 3 days after injection of HCG. Embryos were flushed with modified Dulbecco's phosphate buffered saline and equilibrated with 1.5M-dimethyl sulfoxide (DMSO) or 1.5M-glycerol by 3-step procedure. The freezing rates of the embryos were 1$^{\circ}C$/min from room temperature to -5$^{\circ}C$ and the embryos were seeded at -5$^{\circ}C$. After being held for 3 min at the seeding temperature, the rates were 0.3$^{\circ}C$/min from -5$^{\circ}C$ to -35$^{\circ}C$. From -35$^{\circ}C$ to -7$0^{\circ}C$, the rates were divided into 0.1$^{\circ}C$/min, 1$^{\circ}C$/min and 1$0^{\circ}C$/min, respectively. After being held for 5 min at -7$0^{\circ}C$, the embryos were plunged directly into liquid nitrogen. The embryos were thawed at 4$^{\circ}C$/min and 12$^{\circ}C$/min from -196$^{\circ}C$ to 37$^{\circ}C$, and for 2 min in 37$^{\circ}C$ water bath, respectively. The average number of ovulation points and embryos recovered were 42.7 and 34 appearing 79.5% recovery rate. Eight cell embryos in the embryos recovered were 26.3. The survival rates of embryos according to the freezing rates in the presence of 1.5M-DMSO were 73.5~80.6% at 0.1$^{\circ}C$/min, 75.0~79.5% at 1$^{\circ}C$/min and 52.8~54.7% at 1$0^{\circ}C$/min, but in the presence of 1.5M-glycerol were 62.9~67.6% at 0.1$^{\circ}C$/min, 61.4~68.3% at 1$^{\circ}C$/min and 25.5~30.2% at 1$0^{\circ}C$/min. The survival rates of embryos were not affected by the thawing rates.

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Evaluation of Insecticidal Activity of Plant Extracts against the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae) on Vegetable Plant (배추좀나방(Plutella xylostella)에 대한 식물추출물의 살충력 평가)

  • Choi, I-Jin;Kwon, Hyuk-Hyun;Lee, Han-Ho;Son, Hyoung-Gi;Hong, Sang-Kil;Kang, Jong-Woon;Park, Yu-Soon
    • Korean Journal of Plant Resources
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    • v.26 no.1
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    • pp.19-25
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    • 2013
  • This study focused on the usefulness of plant extracts for a city organic farm. Insecticidal activity of 8 plant species, plants crude extracts by ethanol and water, against third instar larvae (susceptible strain) of Plutella xylostella was evaluated in this study. Ethanol(25%) extracts of plant materials had better insecticidal activity than water extracts at the room temperature. Insecticidal activity were investigated by leaf disc spray method. Five plant extracts (Capsicum annuum, Chelidoniun majus, Leonurus sibiricus, Coptis japonica, Mentha piperascens) showed over 50% insecticidal activity to P. xylostella at the concentration of $1500mg{\cdot}L^{-1}$. Extract of C. annuum showed the highest insecticidal activity (83.3%) against P. xylostella. Phytotoxicity was not observed on Raphanus sativus and Brassica campestris with spray application. Seed germination and growth of two plants were not affected. Extract of C. japonica revealed 73.3% control efficacy against P. xylostella of R. sativus and showed 70% control efficacy against those of B. campestris, respectively, in plastic house. Insecticidal activity against various insects was observed in C. japonica extract as 87.4% for R. sativus and 74.3% for B. campestris in the field. These results suggested that extracts of C. annuum, C. japonica showed the highest insecticidal activity, and could be used as city farm insecticides for organic farming.

Improvement of Mechanical Properties of Mg alloys through Control of Grain Size and Texture (결정립크기와 집합조직제어를 통한 마그네슘 합금의 기계적 성질 개선)

  • Kim, W.J.;Lee, J.B.;Kim, W.Y.;Jeong, H.G.;Park, J.D.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2006.05a
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    • pp.57-58
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    • 2006
  • The effects of lowering ECAP temperature during ECAP process and Post-ECAP annealing on microstructure, texture and mechanical properties of the AZ31 alloys have been investigated in the present study. The as-extruded materials were ECAP processed to 2 passes at 553K prior to subsequent pressing up to 6 passes at 523K or 493K. When this method of lowering ECAP temperature during ECAP was used, the rods could be successfully deformed up to 6 passes without any surface cracking. Grain refinement during ECAP process at 553K might have helped the material to endure further straining at lower deformation temperatures probably by increasing the strain accommodation effect by grain boundary sliding, causing stress relaxation. Texture modification during ECAP has a great influence on the strength of Mg alloys because HCP metals have limited number of slip systems. As slip is most prone to take place on basal planes in Mg at room temperature, the rotation of high fraction of basal planes to the directions favorable for slip as in ECAP decreases the yield stress appreciably. The strength of AZ31 Mg alloys increases with decrease of grain size if the texture is constant though ECAP deformation history is different. A standard positive strength dependence on the grain size for Mg alloys with the similar texture (Fig. 1) supports that the softening of ECAPed Mg alloys (a negative slope) typically observed despite the significant grain refinement is due to the texture modification where the rotation of basal planes occurs towards the orientation for easier slip. It could be predicted that if the original fiber texture is restored after ECAP treatment yielding marked grain refinement, yield stress as high as 500 MPa will be obtained at the grain size of ${\sim}1{\mu}m$. Differential speed rolling (DSR) with a high speed ratio between the upper and lower rolls was applied to alter the microstructure and texture of the AZ31 sheets. Significant grain refinement took place during the rolling owing to introduction of large shear deformation. Grain size as small as $1.4{\mu}m$ could be obtained at 423K after DSR. There was a good correlation between the (0002) pole intensity and tensile elongation. This result indicates that tensile ductility improvement in the asymmetrically rolled AZ31 Mg alloys is closely related to the weakening of basal texture during DSR. Further basal texture weakening occurred during annealing after DSR. According to Hall-Petch relation shown in Fig. 1, the strength of the asymmetrically rolled AZ31 is lower than that of the symmetrically rolled one when compared at the same grain size. This result was attributed to weakening of fiber texture during DSR. The DSRed AZ31, however, shows higher strength than the ECAPed AZ31 where texture has been completely replaced by a new texture associated with high Schmid factors.

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The Retrospective Analysis of Traffic Accident Inpatients in Korean and Western Medicine Hospital (교통사고로 입원한 한방병원과 양방병원의 환자에 대한 후향적 분석)

  • Kim, Young-Jun;Kim, Tae-Ryeong;Woo, Chang-Hoon;Park, Jae-Hong
    • Journal of Korean Medicine Rehabilitation
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    • v.26 no.4
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    • pp.97-105
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    • 2016
  • Objectives The purpose of this study is to investigate traffic accident patients who were admitted to Korean and Western medicine hospital. Methods According to the medicine record from Dong-eui university, 908 traffic accident patients who were admitted to Korean medicine hospital and Western medicine hospital from 1st January 2011 to 31th July 2016 were analyzed. We classified the patients' sex, age, classification of diseases, hospitalization. Results 1. According to age and sex, the rate of male was 35.4%, and that of female was 64.6% in Korean medicine hospital. However, the rate of male was 46.6%, and that of female was 53.4% in Western medicine hospital. 2. According to admission procedures, the most patients (93.3%) went through out-patient department in Korean medicine hospital and 53.5% of patients went through emergency room in Western medicine hospital. 3. According to principal diagnosis, 'sprain and strain of neck level' was 65.4 percentage Korean medicine hospital and 'intracranial injury' was 63.2 percentage in Western medicine hospital. 4. There was no statistical significant in hospitalization periods between male and female in Korean medicine hospital. However, hospitalization periods of female were longer than that of male in Western medicine hospital. 5. There was no correlation between age and hospitalization period in both Korean and Western medicine hospital. 6. Average hospitalization period of Korean medicine hospital patients were shorter than that of Western medicine hospital patients. 7. According to automobile insurance review organization, hospitalization periods were not significantly different in both Korean and Western medicine hospital. Conclusions It is concluded that there is difference of characteristics between inpatients who were admitted to Korean and Western medicine hospital due to traffic accident.

Studies on tussah silkworm, Antheraea pernyi (작잠에 관한 연구)

  • 박병희;송기언;이상풍;박광의
    • Journal of Sericultural and Entomological Science
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    • v.5
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    • pp.25-38
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    • 1965
  • I. Breeding of tussah silkworm(preliminary report). The preliminary examination for bleeding has been carried out since 1963 in tussah silkworms. 1) The strain(l-MG-B)of the heaviest silk quantity was the green silkworm and brown cocoon in univoltine, and the strains(2-G-B, 2-MG-B) of the heaviest silk quantity were also the green silkwom and brown cocoon in bivoltine in both spring and fall in 1965. 2) It looks like the voltinism, the body color and the cocoon color have reached to pure line up to 1965. II. Best place for the winter of tussah pupa. This work was aimed to find out good ways for the winter of tussah pupa. 1) The hatch of bivoltine was better than that of univoltine. 2) The cocoons covered with the leaves were good in the emergence of moth. 3) The cocoons which were kept at natural temperature till the first emergence of moths would show bad in both hatch and emergence. 4) If some of the pupae kept under natural condition were controled at proper temperature for a few days, hatch and laying eggs were best. 5) The best places for the winter were the egg storage and the rearing room. III. Relation between incubation temperature and voltinism. 1) When the tussah pupa are kept at natural temperature during winter, the moths do not come out of the pupa. 2) There is no difference between about 18$^{\circ}C$ and about 25$^{\circ}C$ during incubation in hatching ratio. 3) The tussah silkworms of univoltine in mortality are stronger than that of bivoltine. 4) There is not any relation between voltinism and high or low temperature for pupa and eggs. IV. Induced mutation by gamma-ray and neutron in tussah silkworm. This work was carried out in order to induce the mutation by treating the pupa or the eggs of tussah silkworm with gamma my and neutron. The results obtained are as follows. 1. Though the whole pupa treated with neutron become moths, the moths have no ability to copulate each other. The only moths emerged from pupa treated with neutron, 4000${\gamma}$ are able to lay all un-fertilized eggs, some of which have a hole on the surface and nothing of contents. 2. The non-diapause eggs are treated with neutron in spring, but the hatching ratio is 50∼60 percent, but the whole eggs treated with gamma ray are never hatched. 3. The sensitivity of the pupa to neutron is weaker than that of the eggs. 4. The hatching ratio is in direct proportion to the gamma ray dose. 5. Author find out a new mutant which is excellent in the cocoon quality, so he will do the progeny test next hear.

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A Mutant Arthrospira platensis M20CJK3 Showing Enhanced Growth Rate and Floatation Activity (생장 및 부상성이 향상된 남세균 돌연변이 균주 Arthrospira platensis M20CJK3)

  • Yoo, Chan;Kim, Choong-Jae;Choi, Gang-Guk;Ahn, Chi-Yong;Choi, Jong-Soon;Oh, Hee-Mock
    • Korean Journal of Microbiology
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    • v.45 no.3
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    • pp.268-274
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    • 2009
  • A photosynthetic cyanobacterium Arthrospira platensis, well known for health food supplement, was studied as a target species for atmospheric $CO_2$ removal as well as biomass production. Although the biomass of A. platensis was massively produced in many countries, the recovery cost of its biomass is still high. The purpose of this study was to develop the A. platensis mutant strains which have enhanced growth rate and floatation activity to reduce the recovery cost. A. platensis KCTC AG20590 was treated with 0.24% ethyl methanesulfonate (EMS) for 20 min at room temperature. The mutant strain A. platensis M20CJK3 was finally selected by its morphological and physiological features. The morphology of the mutant A. platensis M20CJK3 was changed from loose-coiled form to tight-coiled form showing short pitch. The growth and $CO_2$ uptake rate of A. platensis M20CJK3 were improved about 15% and 17% compared with A. platensis KCTC AG20590, respectively. The floatation activity of A. platensis M20CJK3 was enhanced in 2-fold compared with that of A. platensis KCTC AG20590. Soluble proteins extracted from two strains were analyzed by two dimensional electrophoresis (2-DE) and MALDI-TOF MS/MS. Among 15 protein spots induced in 2-DE analysis, two spots were the proteins related to photosynthesis and electron transfer system of the other cyanobacteria. As a consequence, it seems that the tight-coiled mutant A. platensis M20CJK3 has an advantage of high growth rate and floatation activity which are beneficial for the mass cultivation and recovery.

Effects of Ventilation Condition and Ventilating Hole Sizes to Improve Quality Onion(Allium cepu. L) under Room Temperature (양파 간이저장시 통풍조건 및 통풍구 재료의 크기가 저장에 미치는 영향)

  • 이찬중;김희대;정은호;김우일;서전규
    • Food Science and Preservation
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    • v.8 no.4
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    • pp.356-361
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    • 2001
  • This study was conducted to improve the storability of onion bulbs by assembly simple house storage and the reduce the rotteness caused by field open storage. Allium cepa cv. Changnyungdeago, late strain was used for the test at the storage condition of natural ventilation of levels 2, forced ventilation of levels 2, field open storage and 75mm, 100mm, and 125mm ventilating holes. Mean tamperature and relative humidity were not significantly different by ventilation conditions. Mean temperature was lower in forced ventilation than that of in natural ventilation and non-ventilation, and relative humidity was a little higher in ventilation treatment than those of the others. Weight loss of onion bulbs were 2.5%, 2.9%, 3%, 4.3% in field open storage, non ventilation, natural ventilation of levels 2 and forced ventilation of levels 2 respectively. Rotting rate in natural ventilation of levels 2 and farced ventilation of levels 2 were 27.7% and 25.4% respectively but 34.6% and 37.8% in non ventilation and field open storage. Therefore, the treatment of ventilation reduced the rotteness of storage onion bulbs. The smaller the size of a ventilating hole, the lower mean temperature was maintained. The relative humidity was some high in July, but didn’t showed significantly difference in August and September. With small size of a ventilating hole, the strong wind velocity was obtained, and wind velocity by position was weaker in the middle part than both ends. Rutting rates in 75㎜, 100㎜, and 125㎜ ventilating holes were 17.9%, 15.3% and 14.1% respectively.

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