• Journal of Microbiology and Biotechnology
• /
• v.12 no.2
• /
• pp.349-353
• /
• 2002

Two recombinant bacteria containing luxAB showed an increased tolerance to stresses associated with lyophilization, when the cells were freeze-dried in the presence of trehalose. In the case of a recombinant, UV2, only $2.5\%$ of the original bioluminescence and $2.7\%$ of the cell viability were restored after 4 h of freeze-drying without trehalose, which implies that the cells were heavily damaged during the dehydration. To improve these losses, trehalose was added before freeze-drying using different modes. Trehalose increased the bioluminescence and the viability of freeze-dried UV2 under all conditions tested, and it was also observed that the addition of trehalose to the cultures (final concentration of 0.08 M) for 15 min before the freeze-drying resulted in the restoration of $45\%$ of the original bioluminescence and $50\%$ of the cell viability. Trehalose also showed a similar efficacy with the other luminescent recombinant, YH9. Therefore, it was tentatively concluded that trehalose played a role as a protective agent in the freeze-drying of bacterial cells.

• Journal of Microbiology and Biotechnology
• /
• v.21 no.12
• /
• pp.1264-1269
• /
• 2011

Interleukin-2 (IL-2) is a vital cytokine secreted by activated T lymphocytes, and plays an important role in the regulation of cellular functions and immunity of animals. In this study, the recombinant duck IL-2 (rduIL-2) was secretory expressed in Pichia pastoris (P. pastoris). The recombinant P. pastoris strain was cultured in shake flasks and then scaled up in a 5.0-l bioreactor. The result showed that the maximal fresh-cell-weight of 594.1 g/l and the maximal $OD_{600}$ of 408 were achieved in the bioreactor. The rduIL-2 was purified by two steps of purification procedures, and approximately 311 mg of rduIL-2/L fermentation supernatant was obtained. SDS-PAGE showed that the purified rduIL-2 constituted a homogeneous band of ~16 kDa or ~14 kDa corresponding to the glycosylated or non-glycosylated duIL-2 protein in size, respectively. The bioactivity of rduIL-2 was determined by lymphocyte proliferation assay. The result indicated that the rduIL-2 greatly promoted the proliferation of ConA-stimulated lymphocytes in vitro. The P. pastoris expression system described here could provide promising, inexpensive, and large-scale production of the rduIL-2, which lays the foundation for development of novel immunoadjuvants to enhance both the immunity of ducks against various infectious pathogens and vaccine efficacy.

• The Journal of Industrial Distribution & Business
• /
• v.9 no.10
• /
• pp.21-37
• /
• 2018

Purpose - This paper explores the contribution lean makes to service levels by addressing lean thinking application in logistics process improvement within a MNC soft drinks manufacturer situated in the United Arab Emirates, and identifies the most significant sources of wastes and their interdependence and highlights key attributes of successful lean implementation. Research design, data, and methodology - This case study examines key logistics employees' perceptions on lean methodology. Results - The results highlight the most significant types of wastes in logistical processes and illustrates ways to eliminate wastes and streamline process flow. The importance of the human role within lean methodology is also identified. Findings suggest that managers should follow both a micro and macro approach when implementing lean practices, as focusing on one waste often leads to ignoring the significance of other wastes. Conclusions - This study highlights the importance of implementing lean at both micro and macro levels and shows how staff involvement increases the efficacy of lean thinking. It offers guidance towards the efficiency of creating value stream mapping processes in situ, which considers different wastes and their interdependence. Managers with limited resources are encouraged to implement lean methodology taking cognizance of these factors.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.10a
• /
• pp.123-123
• /
• 2018

Premenstrual syndrome (PMS) is a common disorder affecting the emotional and physical health of women during certain periods of the menstrual cycle. Many researchers who have previously studied PMS have believed that PMS is associated with changes in sex hormones and serotonin levels at the beginning of the menstrual cycle. However, recent studies suggest that progesterone/estrogen imbalance and elevation of prolactin-induced by dopamine low-secretion play a crucial role in increasing PMS symptoms. Because of this, we have focused on mitigating PMS symptoms through the mechanism of prolactin secretion inhibition by dopamine receptor activation. The inhibition of prolactin secretion by 61-kinds of medicinal herb extracts was investigated in GH3 pituitary cells. Among them, Inula heleniun L. root extract (IHE) showed excellent prolactin secretion inhibitory effect. IHEs were prepared using 30, 50, and 70% ethanol. And the yield, cytotoxicity, dopamine receptor activity and inhibition of prolactin secretion of each extract were measured. Through a series of experiments, we found that prolactin secretion was significantly reduced (P<0.01) by the components present in IHE and that dopamine receptor regulation was possible (P<0.05). Considering yield and safety, we suggest the use of 30% ethanol IHE in the development of PMS symptom relief products.

• Journal of Acupuncture Research
• /
• v.15 no.2
• /
• pp.437-454
• /
• 1998

Osteopotosis is a disease characterized by reduced amount of bone mass leading to enhanced bone flagility. The number of patients with osteoporotic vertebral fracture is increasing and it is one of the leading causes of morbidity in the elderly and postmenopausal women. It is a condition in which bone mass decrease, causing bones to be more susceptible to fracture. A trivial trauma can easily cause one or more bones to break in a person with severe osteoporosis. So it is a major health problem. Pysicians and patients are concerned with the optimum approach to the treatment and prevention of osteoporosis. Until a recent date, many oriental medicine studies were performed to find the preventive and curative efficacy on the osteoporosis, which is differ from therapeutics of Western-medicine. The proper use of Herb-med and role of Accupuncture are issues that have generated major research efforts. This study was carried out to investigate evaluation of clinical and experimental study on the osteoporosis. So, these are to be mentioned in this paper.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.7
• /
• pp.2827-2832
• /
• 2015

Oxidative stress is associated with colon carcinogenesis including aberrant crypt foci (ACF) formation and it plays an important role in pathophysiological changes in cancer cells. The aims of this study were to investigate the effects of dietary unpolished Thai rice (UTR) on ACF formation and dysplastic progression in azoxymethane (AOM)-treated rats. Anti-cancer efficacy of UTR regarding apoptotic induction and oxidative redox status in human colon cancer (CaCo-2) cells was also investigated. Rats given 20% and 70% of UTR in the diet showed significantly and dose-dependently decreased total number of ACF. UTR treatment also was strongly associated with the low percentage of dysplastic progression and mucin depletion. In addition, we found that UTR significantly induced cancer cell apoptosis, increased cellular oxidants, and decreased the level of GSH/GSSG ratio in CaCo-2 cells. Our study suggests that UTR supplementation may be a useful strategy for CRC prevention with the inhibition of precancerous progression, with induction of cancer cell apoptosis through redox alteration.

• Asian Pacific Journal of Cancer Prevention
• /
• v.17 no.8
• /
• pp.3829-3833
• /
• 2016

Inhibition of cancer-associated fibroblasts (CAFs) may improve the efficacy of cancer therapy. Polysaccharide extracted from polygonatum can selectively inhibit the growth of prostate-CAFs (p<0.001) without inhibiting the growth of normal fibroblasts (NAFs). Polysaccharides from polygonatum stimulate autophagy of prostate-CAFs. 3-methyl-adenine(3-MA) is an autophagy inhibitor. 3-MA was added to prostate-CAFs with polysaccharide from polygonatum to determine whether autophagy plays an important role in the restrained effect. Finally, polysaccharide from polygonatum treatment significantly increased the activation of Beclin-1 and LC3, key autophagy proteins. Polysaccharide from polygonatum stimulates autophagy of prostate-CAFs and inhibits prostate-CAF growth, indicating that a novel anti-cancer strategy involves inhibiting the growth of prostate-CAFs.

• Wind and Structures
• /
• v.22 no.1
• /
• pp.107-131
• /
• 2016

The need for a more affordable, reliable, clean and secure energy has led to explorations in non-traditional sources, particularly renewable energies. Wind is one of the cleanest energy sources that plays a significant role in augmenting sustainability. Wind turbines, as energy convertors, are usually tall and slender structures, and depending on their location (inland or offshore), they can be subject to high wind and/or strong wave loadings. These loads can cause severe vibrations with detrimental effects on energy production, structural lifecycle and initial cost. A dissipativity analysis study was carried out to know whether wind turbine towers require damping enhancement or rigidity modifications for vibration suppression. The results suggest that wind turbines are lightly damped structures and damping enhancement is a potential solution for vibration lessening. Accordingly, the paper investigates different damping enhancement techniques for vibration mitigation. The efficacy of tuned mass damper (TMD), tuned liquid column damper (TLCD), tuned sloshing damper (TSD), and viscous damper (VD) to reduce vibrations is investigated. A comparison among these devices, in terms of robustness and effectiveness, is conducted. The VD can reduce both displacement and acceleration responses of the tower, better than other types of dampers, for the same control effort, followed by TMD, TSD, and finally TLCD. Nevertheless, the use of VDs raises concerns about where they should be located in the structure, and their application may require additional design considerations.

• Human Ecology Research
• /
• v.56 no.4
• /
• pp.361-375
• /
• 2018

This study recognizes the changes of the father's role and the importance of family mealtimes as well as examines the individual, family and social contexts surrounding the father's family mealtime. This study analyzed the trend of the father's family mealtime frequency and factors affecting them in order to explore the current conditions of their father's work and family balance through family mealtimes. Data from 492 fathers who have school aged children were collected through an online survey. The overall trend of the frequency of father's family meals showed an average of 2.4 breakfasts and an average of 3.2 dinners per week. The frequency of the father's family meals showed statistically significant differences according to the father's age, children's school age, dual income, weekly working hours, working flexibility, leaving on-time and the priority of work and family. The analysis of hierarchical linear regressions was conducted to determine factors affecting the frequency of father's family mealtime to show that the frequency of father's family mealtime was best explained by father's working conditions and perception variables. Working conditions such as working hours, leaving work on time, working flexibility were significant variables in regards to the father's family mealtime frequency. The father's perception towards parental efficacy, wife's support, and work-family priority were significant factors in regards to the father's family mealtime frequency. This study is meaningful in that it provides basic data on policy making for the compatibility of the father's work and family.

• Journal of Korea Game Society
• /
• v.20 no.4
• /
• pp.111-124
• /
• 2020

This paper analyzed the dialogue language of 'Maple Story' NPC from the perspective of learned optimism. Not only is NPC's language a key skill in artificial intelligence language, it also plays a big role in giving game users fun and desire to achieve. In this paper, as a result of analyzing the dialogue language of NPCs by utilizing the two key dimensions of NPC's learned optimistic explanation form: permanence and pervasiveness, the rate of characters' conversations using optimistic languages was 6%~7%. It was confirmed the important consideration in NPC artificial intelligence development.