• Title/Summary/Keyword: river efflux

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Isolation of Norfloxacin Resistan Escherichia Coli from the Han River and Characterization of Resistance Mechanism

  • Yoosun Jung;Hunjin Hong;Hyeran Nam;Lee, Yeonhee
    • Journal of Microbiology
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    • v.40 no.1
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    • pp.63-69
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    • 2002
  • A total of twenty-five norfloxacin resistant Escherichia coli were isolated from Joongrang-chun stream, a branch of the Han River in Seoul, Korea from May to July in 2000 and their norfloxacin resistance mechanism was characterized for target site mutation, permeability, and efflux pump. Fourteen iso- lates contained the same three mutations, Ser83→Leu and Asp87→Asn in GyrA and Ser90→ lle in ParC. Six isolates had Ser83→Leu and Asp87→Tyr in GyrA and Ser87→lle in ParC while one isolate had Ser83→Leu and Va1103→Ala in GyrA and Ser80→lle in ParC. Two isolates had mutation(s) in GyrA without any mutation in ParC. Two isolates had Ser80→Arg in ParC instead of the commonly found Ser80→lle. Every norfloxacin resistant isolate had an efflux system but the correlation between the efflux activity and MIC was not observed. The amount of OmpF for norfloxacin permeability decreased in resistant isolates compared to the susceptible strains. When amplified polymorphic DNA (RAPD) and pulse field gel electrophoresis (PFGE) were performed, these isolates showed no similarity to each other or clinical isolates.

Evolution and Changes of Coastal Topography due to Jetty Construction at Namdae River Mouth (도류제 건설 후 남대천 하구의 해안선 생성 및 변화)

  • Kim, In Ho;Lee, Seong Dae
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.28 no.3B
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    • pp.315-321
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    • 2008
  • Recently, in the light of environments and utilization, countermeasures to preserve beaches in coastal area are required without depending on such as jetties and breakwaters. The necessity of integrated sand management including not only coastal sediment but also sediment discharge from hinterland rivers is increased so as to establish long-term counterplan for sediment transport. In this regard, the following subjects are examined in this study; efficient ways for discharged sand to be transported from a river to the neighboring coast, the river terrace occurrence and its growth at the river delta, measures to improve storage efficiency of the discharged sand and measures to prevent the sand resources from being discharged into the deep sea during flooding. In recent, A jetty of 260 m length was constructed at Namdae River mouth in the year of 2005 as a countermeasure against the occurrence of sand-bar at river mouth and its close. In this study, a series of numerical experiments were carried out to investigate the characteristics of sediment transport and morphological change due to the construction of jetty at the entrance of Namdae River mouth. Firstly, The sand discharge from Namdae River is quantified by one-dimensional numerical analysis assuming the mixed sand of three different particle diameters. Then, in order to understand the transport behavior of the sand discharge from river and river mouth phenomena the numerical experiments were then conducted to examine the flow behaviors of river efflux and wind generated circulations in coastal area. And, after establishing the numerical model system, which predicts the sea bed changes obtained from the flux model combining with the wave propagation, wave-induced currents and sediment transport models, the sediment transport in the vicinity of Namdae River mouth is analyzed.

Isolation and Characterization of Pseudomonas sp. KM10, a Cadmium- and Mercury-resistant, and Phenol-degrading Bacterium

  • Yoon, Kyung-Pyo
    • Journal of Microbiology and Biotechnology
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    • v.8 no.4
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    • pp.388-398
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    • 1998
  • A bacterium which is resistant to both mercury and cadmium, and also capable of utilizing phenol as a carbon and energy source, was isolated from the Kumho River sediments near Kangchang Bridge, Taegu, Korea. The isolate was labeled Pseudomonas sp. KM10 and characterized. The bacteria grew in 4 mM $CdCl_2$and in $70{\mu}M$ $HgCl_2$. The bacteria efficiently removed over 90% of 1 g/l phenol within 30 h. In the presence of 1.250 g/l phenol, the growth of the microorganism was slightly retarded and the microorganism could not tolerate 1.5 g/l phenol. Curing of plasmid from the bacteria was carried out to generate a plasmidless strain. Subsequent experiments localized the genes for phenol degradation in plasmid and the genes for mercury resistance and cadmium resistance on the chromosome. Dot hybridization and Southern hybridization under low stringent conditions were performed to identify the DNA homology. These results showed significant homologies between the some sequence of the chromosome of Pseudomonas sp. KM10 and merR of Shigella flexneri R 100, and between the some sequence of the chromosome of Pseudomonas sp. KM10 and cadA of Staphylococcus aureus pI258. The mechanism of cadmium resistance was efflux, similar to that of S. aureus pI258 cadA, and the mechanism of mercury resistance was volatilization, similar to that of S. flexneri R100 mer.

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Analysis of Lake Water Temperature and Seasonal Stratification in the Han River System from Time-Series of Landsat Images (Landsat 시계열 영상을 이용한 한강 수계 호수 수온과 계절적 성충 현상 분석)

  • Han, Hyang-Sun;Lee, Hoon-Yol
    • Korean Journal of Remote Sensing
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    • v.21 no.4
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    • pp.253-271
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    • 2005
  • We have analyzed surface water temperature and seasonal stratification of lakes in the Han river system using time-series Landsat images and in situ measurement data. Using NASA equation, at-satellite temperature is derived from 29 Landsat-5 TM and Landsat-7 ETM+ images obtained from 1994 to 2004, and was compared with in situ surface temperature on river-type dam lakes such as Paro, Chuncheon, Euiam, Chongpyong, Paldang, and with 10m-depth temperature on lake-type dam lake Soyang. Although the in situ temperature at the time of satellite data acquisition was interpolated from monthly measurements, the number of images with standard deviation of temperature difference (at-satellite temperature - in situ interpolated temperature) less than $2^{\circ}C$ was 24 on which a novel statistical atmospheric correction could be applied. The correlation coefficient at Lake Soyang was 0.915 (0.950 after correction) and 0.951-0.980 (0.979-0.997 after correction) at other lakes. This high correlation implies that there exist a mixed layer in the shallow river-like dam lakes due to physical mixing from continuous influx and efflux, and the daily and hourly temperature change is not fluctuating. At Lake Soyang, an anomalous temperature difference was observed from April to July where at-satellite temperature is $3-5^{\circ}C$ higher than in situ interpolated temperature. Located in the uppermost part of the Han river system and its influx is governed only by natural precipitation, Lake Soyang develops stratification during this time with rising sun elevation and no physical mixture from influx in this relatively dry season of the year.

Organic Matters Budget and Movement Characteristic in Lake Hoengseong (횡성호의 유기물 수지 및 거동 특성)

  • Joung, Seung-Hyun;Park, Hae-Kyung;Yun, Seok-Hwan
    • Journal of Korean Society on Water Environment
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    • v.28 no.2
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    • pp.238-246
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    • 2012
  • Organic matters budget in Lake Hoengseong were monthly investigated from April 2009 to November 2009. The intense rainfall occurred at between July and August and the hydrological factors were highly varied during the rainfall season. By the concentrated rainfall, the elevation, influx and efflux were sharply increased and the turbid water was also flowed into the middle water column in Lake. The inflow of turbid water increased the nutrient concentrations in water body and this appears to stimulate of phytoplankton regard as the primary productivity of influx of organic matter. Monthly average concentration of dissolved organic carbon (DOC) was generally higher than the particulate organic carbon (POC) concentration in Lake, but Temporal and spatial variation of POC concentration was higher than DOC and the maximum POC concentration was recorded in surface water in August, had the highest phytoplankton biomass. Organic carbon concentration in inflow site was rarely changed during the dry season, but the concentration was rapidly increased by the initial intense rainfall. In organic matters budget, the most of the organic matters was inflowed from the inflow site at rainfall season. Especially, the influx of allochthonous organic matters during the intense rainfall was 72.4% in the total influx organic matters.

Study on the Systematic Technology of Promoting Purification for the Livestock Wastewater and Reuse

  • Okada, Yoshiichi;Shim, Jae-Do;Mitarai, Masahumi;Kojima, Takayuki;Gejima, Yoshinori
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1996.06c
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    • pp.692-700
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    • 1996
  • The objective of this study is to develop a systematic purification plant using the metabolism of aerobic microorganisms. This system is subsequently aerated and continuously removes suspended solids and settling sludges caused by aerating pressure at the bottom of a lower pipe (i.e., Continuous Removal of Suspended solids and Settling sludges, CRSS). The CRSS plants are brought out by introducing fine air bubbles into the liquid phase of a lower pipe in the bio-reactor. These plant uses aeration pipe, with multiple inlets to sweep the floor of bio-reactor tank, instead of the conventional scraper mechanisms. The principal advantage of this system is that it can continuously remove very small or light particles that settles completely within a short time. Once the particles have been floated to the surface, they can be moved into the pipe and collected in the settling tank by sequently aerated pressure. The experimental results shows that about 99.0% of the biochemical oxygen demand(BOD), 99.3% of the suspended solid(SS), 92.3% of the total nitrogen(T-N), 99.0% of the turbidity(TU), 100% of the total coliform(TC)and ammonia was respectively removed during aerobic digestion for 9 days. These result indicates that the CRS S plants are very effective for reduction and deodorization of swine wastewater contaminants, and the efflux from CRS S can either be discharged in the river or used as nutrient solution of formulation for plant growth factories. The developed CRSS plant proved to be flexible and it can simply be adapted to any type of biological waste treatment problem.roblem.

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A Study on Controlling Efflux Sediment Diffusion by Jetty Construction at Small Estuary (소규모 하구에서 토사유출 확산제어에 관한 연구)

  • Park, Sang Kil;Park, Hyun Su;Yoon, Jong Su;Lee, Si Ho
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.5B
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    • pp.483-491
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    • 2009
  • In this study, a jetty construction is taken into account for the reduction of sediment diffusive concentration incoming from the upstream river due to the urbanization and industrial development and to minimize the effects on the coastal ecosystems. The field observation and numerical calculation are conducted to analyze the diffusion zone of sediment concentration in the small estuary and coastal area. The specification of the installed jetty which is able to control the sediment concentration was decided based on the prediction of the dispersion area changes in space and time. The selected size and layout for the jetty design were examined for the dispersion zone by numerical calculation and field observation. As a result, the jetties constructed in the estuary retarded the dispersion rate of sediment concentration, so that the effect area of sediment dispersion was obviously decreased. In addition, the measured field data indicated that the sediment deposition in the inside of dikes could be controlled and the right side area of jetties could be preserved without influx sediment diffusion.

Effects of Hydraulic-hydrological Changes by Monsoon Climate on the Zooplankton Community in Lake Paldang, Korea (몬순기후로 인한 수리수문학적 변화가 팔당호 동물플랑크톤 군집에 미치는 영향)

  • You, Kyung-A;Byeon, Myeong-Seop;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.45 no.3
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    • pp.278-288
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    • 2012
  • The zooplankton community of Lake Paldang, Korea, was investigated on a weekly basis from 2004 to 2006. The seasonal succession of zooplankton community structure was influenced by hydrological factors such as rainfall pattern and efflux in Lake Paldang. According to the monsoon climate, spring, fall and winter had reduced precipitation, so that zooplankton dynamics of the lake showed a typical succession pattern. In spring, small sized and faster growing rotifera rapidly increased, and copepods and cladocera noticeably increased thereafter. Rotifera dominated the zooplankton community, occupying more than 90% of total zooplankton abundance. Among rotifera, Keratella cochlearis was extremely dominant in spring. Copepoda were mainly present as Copepodid and Nauplius. Among cladoceran species, Bosmina longirostris was dominant. In summer, during the rainy season, zooplankton were flushed out by an associated dam. After the rainy season, rotifera increased rapidly when the water column of the lake was stable. During the fall, zooplankton abundance gradually reduced in accordance with decreasing water temperature. However, the occupation rate of copepod (Copepodid, Nauplius) increased relatively. Zooplankton dynamics were influenced by meteorological changes and hydraulic-hydrological factors, because Lake Paldang is a completely closed ecosystem.