Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Isolation of Norfloxacin Resistan Escherichia Coli from the Han River and Characterization of Resistance Mechanism

  • Yoosun Jung (Department of Biology, Seoul Women′s University) ;
  • Hunjin Hong (Department of Biology, Seoul Women′s University) ;
  • Hyeran Nam (Department of Biology, Seoul Women′s University) ;
  • Lee, Yeonhee (Department of Biology, Seoul Women′s University)
  • Published : 2002.03.01
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Abstract

A total of twenty-five norfloxacin resistant Escherichia coli were isolated from Joongrang-chun stream, a branch of the Han River in Seoul, Korea from May to July in 2000 and their norfloxacin resistance mechanism was characterized for target site mutation, permeability, and efflux pump. Fourteen iso- lates contained the same three mutations, Ser83→Leu and Asp87→Asn in GyrA and Ser90→ lle in ParC. Six isolates had Ser83→Leu and Asp87→Tyr in GyrA and Ser87→lle in ParC while one isolate had Ser83→Leu and Va1103→Ala in GyrA and Ser80→lle in ParC. Two isolates had mutation(s) in GyrA without any mutation in ParC. Two isolates had Ser80→Arg in ParC instead of the commonly found Ser80→lle. Every norfloxacin resistant isolate had an efflux system but the correlation between the efflux activity and MIC was not observed. The amount of OmpF for norfloxacin permeability decreased in resistant isolates compared to the susceptible strains. When amplified polymorphic DNA (RAPD) and pulse field gel electrophoresis (PFGE) were performed, these isolates showed no similarity to each other or clinical isolates.

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References

  1. J. Appl. Bacteriol. v.58 A note on antibiotic resistance in the bacterial flora of raw sewage and sewage-polluted river Tigris in Mosul, Iraq Al-Jebouri, M.M https://doi.org/10.1111/j.1365-2672.1985.tb01479.x
  2. JAMA v.269 An outbreak of diarrhea and hemolytic uremic syndrome from Escherichia coli O157:H7 in fresh-pressed apple cider Besser,R.E;S.M.Lett;J.T.Weber;M.P.Dolye;T.J.Barett;J.G.Wells;P.M.Griffin https://doi.org/10.1001/jama.269.17.2217
  3. Antimicrob. Agents Chemother v.40 Contributions of individual mechanisms to fluoroquinolone resistance in 36 Escherichia coli strains isolated from humans and animals Everett, M.J;Y.F. Kin;V. Ricchi;L.J. Piddock
  4. Food Microbiol. v.17 Inactivation of Escherichia coli O157:H7 on surface-uninjured and injured green pepper (Capsicum annuum L.) by chlorine dioxide gas as demonstrated by confocal laser scanning microscopy Han. Y;R.H. Linton;S.S. Nielson;P.E. Nelso https://doi.org/10.1006/fmic.2000.0357
  5. J. Bacteriol. v.174 NotI genomic clevage map of Escherichia coli K-12 strain MG1655 Heath,J.D;J.D.Perkins;B.Sharma;G.M.Weinstock https://doi.org/10.1128/jb.174.2.558-567.1992
  6. Drug v.49 Quinolone mode of action Hooper,D.C https://doi.org/10.2165/00003495-199500492-00004
  7. Appl. Envio. Microbiol. v.65 Genotype analysis of Escherichia coli strains isolated from children and chickens living in close contact Kariuki,S;C. Gilks;J. Kimari;A. Obanda;J. Muyodi;P. Aiyaki;C.A. Hart
  8. Arch. Pharm. Res v.19 Norfloxacin resistance mechanism of E. coli 11 and E. coli 101-clinical isolates of Escherichia coli in Korea Kim, K;S.D. Lee;Y.H. Lee https://doi.org/10.1007/BF02976378
  9. J. Microbiol v.39 Prevalence and antibiotic susceptibility of vancomycin-resistant Enterococci in chicken intestines and fecal samples from healthy young children and intersive are unit patients Kim,S.M;E.S.Shin;C.N.Seong
  10. J. Microbiol. v.37 Characterization of extended-spectrum β-lactamases (ESBL) producing Klebsiella and Enterobacter isolated from sewage plant drain water at Kwang-An in Pusan Lee, H.K
  11. J. Antimicrob. Chemother v.32 The accumulation of five antibacterial agents in porin-deficient mutants of Escherichia coli Mortimer,P.G;L.J.Piddock https://doi.org/10.1093/jac/32.2.195
  12. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard-Fifth Edition v.20 National Committee for Clinical Laboratory Standards
  13. Science v.264 Prevention of drug access to bacterial targets: permeability barriers and active efflux Nikaido, H https://doi.org/10.1126/science.8153625
  14. Curr. Opin. Microbiol. v.1 Multiple antibiotic resistance and efflux Nikaido,H https://doi.org/10.1016/S1369-5274(98)80083-0
  15. J. Appl. Bacteriol. v.74 Studies on the ecology of aquatic bacteria of the Lower Niger Delta: multiple antibiotic resistance among the standard plate count organisms Ogan, M.T;D.E. Nwiika
  16. Appl. Envir. Microbiol v.67 Horizontal transfer of a multi-drug resistance plasmid between coliform bacteria of human and bovine origin in a farm environment Oppegaard,H;T.M. Steinum;Y. Wateson https://doi.org/10.1128/AEM.67.8.3732-3734.2001
  17. Mol. Cells v.6 Norfloxacin resistance mechanism of Escherichia coli 59-a clinical isolate in Korea Park, S.H;S.D. Lee;Y.H. Lee
  18. Drugs v.58 no.Suppl2 Mechanisms of fluoroquinolone resistance:an update 1994-1998 Piddock L.J
  19. Antimicrob. Agents Chemother v.44 Efflux-mediated resistance to fluoroquinolones in Gram-negative bacteria Poole,K https://doi.org/10.1128/AAC.44.9.2233-2241.2000
  20. Appl. Envir. Microbiol v.67 Evidence for extensive resistance gene transfer among Bacteroids spp. and among Bacteroids and other genera in the human colon Shoemaker,N.B;H. Vlamakis;K. Hayes;A.A. Salyers https://doi.org/10.1128/AEM.67.2.561-568.2001
  21. Cell v.63 New topoisomerase essential for chromosome segregation in Escherichia coli Suzuki, H
  22. Agents Chemother v.44 Characterization of fluoroquinolone resistance among veterinary isolates of avian Escherichia coli Antimicrob White,D.G;L.J.Piddock;J.J.Maurer;S.Zhao;V.Ricci;S.G.Thayer https://doi.org/10.1128/AAC.44.10.2897-2899.2000