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Isolation of Norfloxacin Resistan Escherichia Coli from the Han River and Characterization of Resistance Mechanism  

Yoosun Jung (Department of Biology, Seoul Women′s University)
Hunjin Hong (Department of Biology, Seoul Women′s University)
Hyeran Nam (Department of Biology, Seoul Women′s University)
Lee, Yeonhee (Department of Biology, Seoul Women′s University)
Publication Information
Journal of Microbiology / v.40, no.1, 2002 , pp. 63-69 More about this Journal
Abstract
A total of twenty-five norfloxacin resistant Escherichia coli were isolated from Joongrang-chun stream, a branch of the Han River in Seoul, Korea from May to July in 2000 and their norfloxacin resistance mechanism was characterized for target site mutation, permeability, and efflux pump. Fourteen iso- lates contained the same three mutations, Ser83→Leu and Asp87→Asn in GyrA and Ser90→ lle in ParC. Six isolates had Ser83→Leu and Asp87→Tyr in GyrA and Ser87→lle in ParC while one isolate had Ser83→Leu and Va1103→Ala in GyrA and Ser80→lle in ParC. Two isolates had mutation(s) in GyrA without any mutation in ParC. Two isolates had Ser80→Arg in ParC instead of the commonly found Ser80→lle. Every norfloxacin resistant isolate had an efflux system but the correlation between the efflux activity and MIC was not observed. The amount of OmpF for norfloxacin permeability decreased in resistant isolates compared to the susceptible strains. When amplified polymorphic DNA (RAPD) and pulse field gel electrophoresis (PFGE) were performed, these isolates showed no similarity to each other or clinical isolates.
Keywords
antibiotic environmental isolate; Escherichia Coli; MDR; norfloxacin; quinolone; resistance;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 4  (Related Records In Web of Science)
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