• Title/Summary/Keyword: ring structure

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Phosphorylation-Dependent Septin Interaction of Bni5 is Important for Cytokinesis

  • Nam, Sung-Chang;Sung, Hye-Ran;Kang, Seung-Hye;Joo, Jin-Young;Lee, Soo-Jae;Chung, Yeon-Bok;Lee, Chong-Kil;Song, Suk-Gil
    • Journal of Microbiology
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    • v.45 no.3
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    • pp.227-233
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    • 2007
  • In budding yeast, septin plays as a scaffold to recruits protein components and regulates crucial cellular events including bud site selection, bud morphogenesis, Cdc28 activation pathway, and cytokinesis. Phosphorylation of Bni5 isolated as a suppressor for septin defect is essential to Swe1-dependent regulation of bud morphogenesis and mitotic entry. The mechanism by which Bni5 regulates normal septin function is not completely understood. Here, we provide evidence that Bni5 phosphorylation is important for interaction with septin component Cdc11 and for timely delocalization from septin filament at late mitosis. Phosphorylation-deficient bni5-4A was synthetically lethal with $hof1{\Delta}$. bni5-4A cells had defective structure of septin ring and connected cell morphology, indicative of defects in cytokinesis. Two-hybrid analysis revealed that bni5-4A has a defect in direct interaction with Cdc11 and Cdc12. GFP-tagged bni5-4A was normally localized at mother-bud neck of budded cells before middle of mitosis. In contrast, at large-budded telophase cells, bni5-4A-GFP was defective in localization and disappeared from the neck approximately 2 min earlier than that of wild type, as evidenced by time-lapse analysis. Therefore, earlier delocalization of bni5-4A from septin filament is consistent with phosphorylation-dependent interaction with the septin component. These results suggest that timely de localization of Bni5 by phosphorylation is important for septin function and regulation of cytokinesis.

Cycloaddition and Crosslinking Reactions of CO2 and Glycidyl Methacrylate using Ionic Liquid (이온성 액체를 이용한 CO2와 glycidyl methacrylate의 고리화 첨가 및 고분자 가교 반응)

  • Kim, Dong Hyun;Kang, Tae Won;Lee, Jong Jib;Ko, Young Soo
    • Korean Chemical Engineering Research
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    • v.51 no.3
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    • pp.342-346
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    • 2013
  • In this study the cycloaddition of glycidyl methacrylate (GMA) and $CO_2$ using ionic liquid as catalyst was performed for the technology of $CO_2$ reduction. The structure of synthesized cyclic carbonate, [2-oxo-1,3-dioxolan-4-yl]methacrylate (DOMA) was analyzed and confirmed by FT-IR and $^1H$-NMR. The change in conversion with respect to reaction time was investigated using $^1H$-NMR. Interestingly, the ionic polymerization of vinyl groups and crosslinking reaction between cyclic carbonate rings of DOMA were observed following completion of cycloaddition.

A Stripline 10-Way Power Divider for the Feed Network of an S-band Linear Array Antenna (S-대역 선형 배열 안테나의 급전 회로를 위한 스트립라인 10-출력 전력분배기)

  • Park, Il-Ho;Kim, Rak-Young;Park, Jung-Yong;Jeong, Myung-Deuk;Kim, Dong-Wook
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.20 no.3
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    • pp.280-288
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    • 2009
  • In this paper, a high-power and low-loss stripline 10-way power divider is designed and fabricated fur the feed network of an S-band linear array antenna with Chebyshev current distribution which has a narrow beam width and low side lobe level(SLL) of 35 dB or more. The unit cell of the power divider is based on a T-junction power divider and the whole divider is comprised of the cascaded unit cells. The multi-stage impedance transformer and modified ring hybrid are used in designing the power divider for performance improvement. And the reflection loss and insertion loss are improved by modifying a connector structure for a coaxial-to-stripline transition.

A PHOTOELASTIC STRESS ANALYSIS ON TOOTH SUPPORTING STRUCTURE AND RESIDUAL RIDGE ACCORDING TO DENTURE DESIGN FOR REMAINING MANDIBULAR CANINES (하악 견치 잔존시 의치 설계에 따른 치아 지지조직 및 잔존치조제의 광탄성 응력분석)

  • Kim, Il-Pyung;Koh, Joon-Won;Jin, Tai-Ho
    • The Journal of Korean Academy of Prosthodontics
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    • v.35 no.3
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    • pp.486-503
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    • 1997
  • The purpose of this study was to evaluate the stress patterns developed in abutment and residual ridge according to removable denture design in case of remaining mandibular canines. The removable denture designs in this study were as foolows : 1. Removable partial denture with non-splinted abutment 2. Removable partial denture with splinted abutment 3. Overdenture with telescopic crown 4. Overdenture with O-Ring attachment 5. Overdenture with combination bar attachment Photoelastic stress analysis was used to record the isochromatic fringe patterns and to calculate principal stress components at measuring points. The results were as follows : 1. In case of removable partial denture with non-splinted abutment, stress of root area at the loaded side was the largest. No significant differences in stress of root area were observed between loaded side and unloaded side. 2. No significant differences in stress of residual ridge at the loaded side were observed between removable partial denture with splinted abutment and removable partial denture with non-splinted abutment. 3. In case of combination bar attachment retained overdenture, stress of root area was the largest and in case of telescopic crown retained overdenture, stress of root area was the lowest. 4. In case of attachment retained overdenture, stress of residual ridge was lower but stress of root area was larger than in case of removable partial denture.

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Electroplating process for the chip component external electrode

  • Lee, Jun-Ho
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2000.11a
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    • pp.1-2
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    • 2000
  • In chip plating, several parameters must be taken into consideration. Current density, solution concentration, pH, solution temperature, components volume, chip and media ratio, barrel geometrical shape were most likely found to have an effect to the process yields. The 3 types of barrels utilized in chip plating industry are the onventional rotating barrel, vibrational barrel(vibarrel), and the centrifugal type. Conventional rotating barrel is a close type and is commonly used. The components inside the barrel are circulated by the barrel's rotation at a horizontal axis. Process yield has known to have higher thickness deviation. The vibrational barrel is an open type which offers a wide exposure to electrolyte resulting to a stable thickness deviation. It rotates in a vertical axis coupled with multi-vibration action to facilitate mixed up and easy transportation of components. The centrifugal barrel has its plated work centrifugally compacted against the cathode ring for superior electrical contact with simultaneous rotary motion. This experiment has determined the effect of barrel vibration intensity to the plating thickness distribution. The procedures carried out in the experiment involved the overall plating process., cleaning, rinse, Nickel plating, Tin-Lead plating. Plating time was adjusted to meet the required specification. All other parameters were maintained constant. Two trials were performed to confirm the consistency of the result. The thickness data of the experiment conducted showed thatbthe average mean value obtained from higher vibrational intensity is nearer to the standard mean. The distribution curve shown has a narrower specification limits and it has a reduced variation around the target value. Generally, intensity control in vi-barrel facilitates mixed up and easy transportation of components. However, it is desirable to maintain an optimum vibration intensity to prevent solution intrusion into the chips' internal electrode. A cathodic reaction can occur in the interface of the external and internal electrode. 2H20 + e $\rightarrow$M/TEX> 20H + H2.. Hydrogen can penetrate into the body and create pressure which can cause cracks. At high intensity, the chip's motion becomes stronger, its contact between each other is delayed and so plating action is being controlled. However, the strong impact created by its collision can damage the external electrode's structure there by resulting to bad plating condition.

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The Development of a 20MW PWM Driver for Advanced Fifteen-Phase Propulsion Induction Motors

  • Sun, Chi;Ai, Sheng;Hu, Liangdeng;Chen, Yulin
    • Journal of Power Electronics
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    • v.15 no.1
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    • pp.146-159
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    • 2015
  • Since the power capacity needed for the propulsion of large ships is very large, a multiphase AC induction propulsion mode is generally adopted to meet the higher requirements of reliability, redundancy and maintainability. This paper gives a detailed description of the development of a 20MW fifteen-phase PWM driver for advanced fifteen-phase propulsion induction motors with a special third-harmonic injection in terms of the main circuit hardware, control system design, experiments, etc. The adoption of the modular design method for the main circuit hardware design can make the enclosed mechanical structure simple and maintainable. It can also avoid the larger switch stresses caused by the multiple turn on of the IGBTs in conventional large-capacity converter systems. The use of the distributed controller design method based on a high-speed fiber-optic ring net for the control system can overcome such disadvantages as the poor reliability and long maintenance times arising from the conventional centralized controller which is designed according to point-to-point communication. Finally, the performance of the 20MW PWM driver is verified by experimentation on a new fifteen-phase induction propulsion motor.

Capillary Flow in Different Cells of Thuja orientalis, Gmelina arborea, Phellodendron amurense

  • Chun, Su Kyoung
    • Journal of the Korea Furniture Society
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    • v.28 no.3
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    • pp.248-258
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    • 2017
  • A study was carried out to observe the 1% aqueous safranine solution flow speed in longitudinal and radial directions of softwood Thuja orientalis L., diffuse-porous wood Gmelina arborea Roxb., and ring-porous wood Phellodendron amurense Rupr., Longitudinal flow was considered from bottom to top while the radial flow was considered from bark to pith directions. In radial direction, ray cells and in longitudinal direction tracheids, vessel and wood fiber were considered for the measurement of liquid penetration speed at less than 12% moisture contents(MC). The variation of penetration speed for different species was observed and the reasons behind for this variation were explored. The highest radial penetration depth was found in ray parenchyma of T. orientalis but the lowest one was found in ray parenchyma of P. amurense. The average liquid penetration depth in longitudinal trachied of T. orientalis was found the highest among all the other cells. The penetration depth in fiber of G. arborea was found the lowest among the other longitudinal cells. It was found that cell dimension and also meniscus angle of safranine solution with cell walls were the prime factors for the variation of liquid flow speed in wood. Vessel was found to facilitate prime role in longitudinal penetration for hardwood species. The penetration depth in vessel of G. arborea was found highest among all vessels. Anatomical features like ray parenchyma cell length and diameter, end-wall pits number were found also responsible fluid flow differences. Initially liquid penetration speed was high and the nit gradually decreased in an uneven rate. Liquid flow was captured via video and the penetration depths in those cells were measured. It was found that even in presence of abundant rays in hardwood species, penetration depth of liquid in radial direction of softwood species was found high. Herein the ray length, lumen area, end wall pit diameter determined the radial permeability. On the other hand, vessel and fiber structure affected the longitudinal flow of liquids. Following a go-stop-go cycle, the penetration speed of a liquid decreased over time.

The Fine Structure of the Sperm Ball and Sperm of Urechis unicinctus and Immunogold Localization of $\alpha-Tubulin$ (개불(Urechis unicinctus) Sperm Ball과 정자의 미세구조와 금 입자 면역 반응에 의한 $\alpha-Tubulin$의 분포)

  • Shin, Kil-Sang
    • Applied Microscopy
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    • v.28 no.2
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    • pp.193-205
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    • 1998
  • The Urechis unicinctus sperm and spermatogenic cells prepared from the testis are investigated to identify $\alpha-tubulin$ of axoneme microtubules using mouse monoclonal $anti-\alpha-tubulin$ as the first Ab and Gold(10nm) conjugated goat anti-mouse IgG as the Ab marker. The Ag-Ab reaction analyzed excellently the localization of $\alpha-tubulin$ and the gold particles incorporated with the proximal and distal centrioles, manchette microtubules, and flagellum. The gold particles can be also observed in the spermatogenic cells while the cells are still in sperm ball which is composed of a somatic cell and spermatogenic cells. The sperm ball is the functional unit of sperm production in U unicinctus testis. The spermatids are developed from the spermatogenic cells in the sperm ball and released into the testis cavity through a cortical cytoplasmic opening. The spermatid architectures are similar with the mature sperm of the testis cavity in aspects of shape of discoid acrosome, degree of nuclear condensation and ring type of mitochondrion. However, the distal centriole connecting with the flagella can be observed from the mature sperm while the both proximal and distal centrioles reveal only in the spermatids. The proximal centriole is directly connected with nuclear outer membrane during the stage of nuclear condensation and oriented perpendicularly to the distal centriole whose axis coinciding with the longitudinal axis of the spermatozoon. There are indications that the distal centriole is intimately associated with the polymerization of the flagellum. The manchette microtubules appear during spermatid development but the mature sperm have round head and no conspicuous middle piece.

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CoMFA and CoMSIA Analysis on the Selective Fungicidal Activity of N-phenyl-D-phenylthionocarbamate Analogues against Resistant and Sensitive Gray Mold (Botrytis cinerea) (저항성 및 감수성 잿빛곰팡이병균(Botrytis cinerea)에 대한 N-Phenyl-O-phenylthionocarbamate 유도체들의 선택적인 살균활성에 관한 CoMFA 및 CoMSIA 분석)

  • Soung, Min-Gyu;Sung, Nack-Do
    • The Korean Journal of Pesticide Science
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    • v.11 no.3
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    • pp.138-143
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    • 2007
  • The relationships between three dimensional quantitative structure and activity relationships (3D-QSARs) for the selective fungicidal function between N-phenyl substituents of N-phenyl-O-phenyl-thionocarbamate derivatives analogues and their the fungicidal activities against resistant (RBC) and sensitive (SBC) gray mold (Botrytis cinerea) were studied quantitatively using CoMFA and CoMSIA methods. The statistical values of optimized CoMSIA (M7) model were better ($r^2$ & $q^2=CoMSIA{\gg}CoMFA$) than that of CoMFA (M5) model. And the factor influencing of the selective between the fungicidal activity against RBC and SBC was dependent on electrostatic field of CoMFA (M5) model. Therefore, it is predicted that, from the CoMSIA contour maps of CoMSIA (M7) model, the selectivity will be improved by the H-bond donor that is with negatively charged favored group at meta-position on the N-phenyl ring.

Distributed Mutual Exclusion Algorithm for Maintaining Tree-Height Balance in Mobile Computing Environments (이동 컴퓨팅 환경에서 트리 높이의 균형을 유지하는 상호 배제 알고리즘)

  • Kim, Hyeong-Sik;Eom, Yeong-Ik
    • Journal of KIISE:Computer Systems and Theory
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    • v.26 no.10
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    • pp.1206-1216
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    • 1999
  • 이동 호스트란 시간과 공간의 제약 없이 필요한 기능을 수행하고, 또한 원하는 정보에 접근할 수 있는 휴대용 컴퓨터를 일컫는다. 이동 컴퓨팅 환경이란 이러한 이동 호스트들을 지원할 수 있도록 구성된 분산 환경이다. 따라서 이동 컴퓨팅 환경에서의 분산 알고리즘은 정적 호스트만을 고려한 기존의 분산 환경에서와는 다르게 이동 호스트의 여러 가지 특성들을 고려하여 제안되어야 한다. 즉, 이동 컴퓨팅 환경의 도래로 인하여 이동성과 휴대성, 그리고 무선 통신과 같은 특성들을 고려한 새로운 분산 상호 배제 알고리즘이 필요하다. 이제까지 이동 컴퓨팅 환경에서의 상호 배제 알고리즘은 토큰 링 구조에 기반을 두고 설계되었다. 토큰 링 구조는 이동 호스트들의 위치를 유지하기 위하여 높은 비용을 필요로 하는 단점을 가지고 있다. 본 논문에서는 균형 높이 트리(height-balanced tree)라는 새로운 모형을 제안함으로써 정적 분산 환경과 이동 분산 환경이 혼합된 환경에서 상호 배제 비용을 감소시킬 수 있는 새로운 알고리즘을 제안하며, 각 경우에 있어서의 비용을 산출하고 평가한다.Abstract The mobile host is a potable computer that carries out necessary functions and has the ability to access desirable informations without any constraints in time and space. Mobile computing environment is a distributed environment that is organized to support such mobile hosts. In that environment, distributed algorithms of which environment not only with static hosts but with mobile host's several properties should be proposed. With the emergence of mobile computing environments, a new distributed mutual exclusion method should be required to consider properties mobile computing system such as mobility, portability, and wireless communication. Until now, distributed mutual exclusion methods for mobile computing environments are designed based on a token ring structure, which have the drawbacks of requiring high costs in order to locate mobile hosts. In this paper, we propose a distributed mutual exclusion method that can reduce such costs by structuring the entire system as a height-balanced tree for static distributed networks and for networks with mobile hosts. We evaluated the operation costs in each case.