• Mycobiology
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• v.45 no.3
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• pp.160-171
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• 2017

Larvae of Bradysia agrestis, an insect vector that transports plant pathogens, were sampled from geographically isolated regions in Korea to identify their cutaneous fungal and bacterial flora. Sampled areas were chosen within the distribution range of B. agrestis; each site was more than 91 km apart to ensure geographical segregation. We isolated 76 microbial (fungi and bacteria) strains (site 1, 29; site 2, 29; site 3, 18 strains) that were identified on the basis of morphological differences. Species identification was molecularly confirmed by determination of universal fungal internal transcribed spacer and bacterial 16S rRNA gene sequences in comparison to sequences in the EzTaxon database and the NCBI GenBank database, and their phylogenetic relationships were determined. The fungal isolates belonged to 2 phyla, 5 classes, and 7 genera; bacterial species belonged to 23 genera and 32 species. Microbial diversity differed significantly among the geographical groups with respect to Margalef's richness (3.9, 3.6, and 4.5), Menhinick's index (2.65, 2.46, and 3.30), Simpson's index (0.06, 0.12, and 0.01), and Shannon's index (2.50, 2.17, and 2.58). Although the microbial genera distribution or diversity values clearly varied among geographical groups, common genera were identified in all groups, including the fungal genus Cladosporium, and the bacterial genera Bacillus and Rhodococcus. According to classic principles of co-evolutionary relationship, these genera might have a closer association with their host insect vector B. agrestis than other genera identified. Some cutaneous bacterial genera (e.g., Pseudomonas) displaying weak interdependency with insect vectors may be hazardous to agricultural environments via mechanical transmission via B. agrestis. This study provides comprehensive information regarding the cutaneous microflora of B. agrestis, which can help in the control of such pests for crop management.

• Korean Journal of Plant Resources
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• v.23 no.4
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• pp.261-273
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• 2010

This study intended to investigate the environmental factors including soil and vegetation in order to understand the environmental and ecological characteristics of seven different habitats of Viola websteri. These habitats, according to investigations, are mostly located on the slope of mountains facing north at an altitude of 343 m to 991 m above sea level with angle of inclination from 1 degree to 33 degrees. The type of soil is mostly sandy loam and the average field capacity of soil is 28.97%. Their average organic matter is 16.63%, soil pH 5.62, and available phosphorus is 14.75%. A total of 133 vascular plants are identified in 18 quadrates of seven habitats. Dominant species of woody plants in seven habitats are represented as Acer pictum subsp. mono and Quercus mongolica in tree layer, and Acer pictum subsp. mono in subtree layer. Importance value of Viola websteri is 9.66%, as regards the herbaceous layer, and five highly ranked species such as Meehania urticifolia(8.53%), Pseudostellaria heterophylla(6.51%), Hylomecon vernalis(5.15%), Oxalis obtriangulata (4.52%), and Pseudostellaria davidii(4.15%) are considered to be an affinity with Viola websteri in their habitats. The degree of their average species diversity is 1.32, and that of dominance and evenness are 0.08 and 0.89, respectively. Correlation coefficients analysis based on environmental factors, vegetation and soil analysis shows that the coverage of Viola websteri is correlated with silt and sand ratio, and coverage of tree layers are correlated with species richness and altitude. Cluster analysis based on vegetation structure of each habitats are forms a three groups.

• Journal of Korean Medicine for Obesity Research
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• v.19 no.2
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• pp.97-105
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• 2019

Objectives: We investigated differences between the tracheostomized and the non-tracheostomized stroke patients through microbiological analysis for the purpose of preliminary explorations of full-scale clinical research in the future. Methods: We collected tracheal aspirates samples from 5 stroke patients with tracheostomy and expectorated sputum samples from 5 stroke patients without tracheostomy. Genomic DNA from sputum samples was isolated using QIAamp DNA mini kit. The sequences were processed using Quantitative Insights into Microbial Ecology 1.9.0. Alpha-diversity was calculated using the Chao1 estimator. Beta-diversity was analyzed by UniFrac-based principal coordinates analysis (PCoA). To confirm taxa with different abundance among the groups, linear discriminant analysis effect size analysis was performed. Results: Although alpha-diversity value of the tracheostomized group was higher than that of the non-tracheostomized group, there was no statistically significant difference. In PCoA, clear separation was seen between clusters of the tracheostomized group and that of the non-tracheostomized group. In both groups, Bacteroidetes, Proteobacteria, Fusobacteria, Firmicutes, Actinobacteria were identified as dominant in phylum level. In particular, relative richness of Proteobacteria was found to be 31% more in the tracheotomized group (36.6%) than the non-tracheostomized group (5.6%)(P<0.05). In genus level, Neisseria (24%), Prevotella (17%), Streptococcus (13%), Fusobacteria (11%), Porphyromonas (7%) were identified as dominant in the tracheostomized group. In the non-tracheostomized group, Prevotella (38%), Veillonella (20%), Neisseria (9%) were genera that found to be dominant. Conclusions: It is meaningful in that the tracheostomized group has been identified a higher rate of microbiotas known as pathogenic in respiratory diseases compared to the non-tracheostomized group, confirming the possibility that the risk of opportunity infection may be higher.

• Journal of Life Science
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• v.25 no.3
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• pp.323-328
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• 2015

The study of microbial diversity and richness in soil samples from a volcanic island named Ulleungdo, located east of South Korea. The soil bacterial communities on the Ulleungdo were analyzed using pyrosequencing method based on 16S rRNA gene. There were 1,613 operational taxonomic units (OUT) form soil sample. From results of a BLASTN search against the EzTaxon-e database, the validated reads (obtained after sequence preprocessing) were almost all classified at the phylum level. Proteobacteria was the most dominant phylum with 48.28%, followed by acidobacteria (26.30%), actionbacteria (6.89%), Chloroflexi (4.58), Planctomycetes (4.56%), Nitrospirae (1.83%), Bacteroidetes (1.51%), Verrucomicrobia (1.48%), and Gemmatimonadetes (1.11%). α-proteobacteria was the most dominant class with 36.07% followed by Acidobacteria_c (10.65%), Solibacteres (10.64%), δ-proteobacteria (4.42%), γ-proteobacteria (4.29%), Planctomycetacia (4.16%), Actinobacteria_c (4.00%), Betaproteobacteria (3.50%), EU686603_c (2.97%), Ktedonobacteria (2.91%), Acidimicrobiia (1.32%), Verrucomicrobiae (1.27%), Gemmatimonadetes_c (1.11%), Sphingobacteria (1.09%), and GU444092_c (1.06%). Bradyrhizobiaceae was the most dominant family with 22.83% followed by Acidobacteriaceae (10.62%), EU445199_f (5.72%), Planctomycetaceae (4.03%), Solibacteraceae (3.63%), FM209092_f (3.58%), Steroidobacter_f (2.81%), EU686603_f (2.73%), Hyphomicrobiaceae (2.33%), Ktedonobacteraceae (1.75%), AF498716_f (1.46%), Rhizomicrobium_f (1.03%), and Mycobacteriaceae (1.01%). Differences in the diversity of bacterial communities have more to do with geography than the impact on environmental factors and also the type of vegetation seems to affect the diversity of bacterial communities.

• Journal of Life Science
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• v.17 no.7 s.87
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• pp.937-943
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• 2007

The fish fauna and weight-length relationship in Andong reservoir were investigated from May 2005 to March 2006. During surveyed period, 2,540 individuals 20 species belonging to 7 families were collected. Dominant species were Hemibarbus labeo(19.6%) and sub-dominant species were Carassius cuvieri (19.2%). The korea endemic species were Hemiculter eigenmanni(7.1%), Squalidus chankaensis tsuchigae (0.1 %), Corroperca herzi (0.1 %) and introduced fishes were Carassius cuvieri (19.21 %) Lepomis macrochirus (4.2%), Micropterus salmoides (0.2%). Dominace, species diversity, species richness, species even-ness were 0.39, 3.16, 2.42, 0.73 respectively. The values of the exponent b in the weight-length relation-ship $W=aL^{b}$ ranged from 2.9162(H. labeo, $R^{2}$=0.9567) to 3.2509(Zacco pla쇼pus, $R^{2}$=0.9928) and the median value was 3.1079, whereas 50% of the values ranged between about 3.05 to 3.20. The Condition factor(K) showed good state of health in the surveyed species (excepts H. labeo and Cyprinus carpio).

• Journal of Wetlands Research
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• v.16 no.3
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• pp.337-346
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• 2014

This study was carried out to investigate the terrestrial diurnal insects in the Donggang river neighborhood. A total of 455 species belonging to 117 families of 16 orders were identified from 3,001 collected terrestrial insects around the Donggang river. Species richness showed as follows; Coleoptera (166 species, 34.48%), Hemiptera (11 species, 24.0%), Lepidoptera (68 species, 14.95%), Diptera (40 species, 8.79%), Hymenoptera (28 species, 6.15%), Orthoptera (18 species, 3.96%), Odonata (7 species, 1.54%), and others (3.74%). The Coleoptera consisted of the most number of individuals (33.2%), followed by 951 Hemiptera (31.7%), 662 Lepidoptera (20.7%), 155 Orthoptera (5.2%), 100 Diptera (3.3%), 87 Hymenoptera (2.9%), 30 Odonata (1.0%) and others (2.0%). Among them, 1 endangered species of Ministry of Environment, 1 Critically Endangered Korean species, 7 Vulnerable Korean species (VU), and also 2 Near Threatened Korean species were surveyed in the Donggang river neighborhood.

• Journal of Microbiology and Biotechnology
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• v.28 no.6
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• pp.976-986
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• 2018

Knowledge about the gut bacterial communities associated with insects is essential to understand their roles in the physiology of the host. In the present study, the gut bacterial communities of a laboratory-reared insecticide-susceptible (IS), and a field-collected insecticide-resistant (IR) population of a major rice pest, the brown planthopper Nilaparvata lugens, were evaluated. The deep-sequencing analysis of the V3 hypervariable region of the 16S rRNA gene was performed using Illumina and the sequence data were processed using QIIME. The toxicological bioassays showed that compared with the IS population, IR population exhibited 7.9-, 6.7-, 14.8-, and 18.7-fold resistance to acephate, imidacloprid, thiamethoxam, and buprofezin, respectively. The analysis of the alpha diversity indicated a higher bacterial diversity and richness associated with the IR population. The dominant phylum in the IS population was Proteobacteria (99.86%), whereas the IR population consisted of Firmicutes (46.06%), followed by Bacteroidetes (30.8%) and Proteobacteria (15.49%). Morganella, Weissella, and Enterococcus were among the genera shared between the two populations and might form the core bacteria associated with N. lugens. The taxonomic-to-phenotypic mapping revealed the presence of ammonia oxidizers, nitrogen fixers, sulfur oxidizers and reducers, xylan degraders, and aromatic hydrocarbon degraders in the metagenome of N. lugens. Interestingly, the IR population was found to be enriched with bacteria involved in detoxification functions. The results obtained in this study provide a basis for future studies elucidating the roles of the gut bacteria in the insecticide resistance-associated symbiotic relationship and on the design of novel strategies for the management of N. lugens.

• Journal of Korean Society of Forest Science
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• v.88 no.3
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• pp.342-351
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• 1999

The forest vegetation developing on Mt. Inwang, an isolated forestland in Seoul, Korea was analyzed phytosociologically and its spatial distribution mapped out. Using the vegetation map, The characteristics of landscape structures in terms of the number and size of patches are discussed. Forest vegetation of the study area was classified into ten communities, ten groups, and eighty subgroups. Landscape element types were classified into secondary forests, relict communities, introduced plantations, and other elements including urbanized area. Pinus densiflora community, natural forest and Robinia pseudo-acacia community, plantation, formed matrix and some secondary forest elements, relict communities and the ether plantations of small size tended to distribute as small patches in such matrix. The number of patches per unit area in secondary forest elements was more than that in plantation elements. The result in patch size was vice versa. The vascular plant species richness of the landscape element types in Mt. Inwang was found to he positively related to their size. As the results of landscape ecological analyses, it was estimated that differentiation of patches recognized in community level would be related to human interference a and those in subordinate levels to natural process such as progression of succession.

• Korean Journal of Environment and Ecology
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• v.29 no.6
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• pp.884-894
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• 2015

To examine the relationship between microphytobenthos biomass and the condition index of Ruditapes philippinarum (manila clam), field observations were conducted seasonally at the tidal flats of Jeongsanpo and Hwangdo, Taean, Korea from February to November, 2012. A total of 122 species of microphytobenthos were identified over the study period with 85 species (30-45 species in season) at Jeongsanpo and 92 species (32-57 species) at Hwangdo. Chlorophyll a concentrations and cell number of microphytobenthos were $79.75mg/m^2$ and $3,255cells/cm^2$ at Jeongsanpo, and $151.50mg/m^2$ and $15,943cells/cm^2$ at Hwangdo, respectively. The dominant species were slightly different: Cylindrotheca closterium, Fallacia forcipata, Fogedia sp., Gyrosigma sp., and Navicula sp. at Jeongsanpo and C. closterium, Detonula pumila, Diploneis sp., Navicula sp. and Merismopedia sp. at Hwangdo tidal flat. Paralia sulcata was the representative species based on cell number at the two study sites. The number of microphytobenthos identified from the digestive organs of manila clams seasonally varied from 18 to 31 species at Jeongsanpo and dominant genus were Amphora, Navicula, Nitzschia and Paralia sulcata. At Hwangdo, the species number of microphytobenthos found in the digestive organs of manila clams were in the range of between 19 and 25 species in season and the dominant genus were Actinocyclus, Amphora, Coscinodiscus, Diploneis, Gyrosigma, Navicula, and Diploneis. The condition index of manila clams were greater at Hwangdo (0.57) than at Jeongsanpo (0.42). Present results could support that the condition index of manila clams is positively correlated with the species richness and chlorophyll a contents of microphytobenthos.

• Korean Journal of Environment and Ecology
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• v.32 no.1
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• pp.39-46
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• 2018

Breeding bird surveys provide the reference information for understanding bird community structure and function in the ecosystem and conserving biodiversity. Recent rapid change of biodiversity due to climate change and other factors has made it necessary to utilize acoustic sensors for accuracy and spatial expansion of forest bird survey data and for time-series monitoring of forest birds. The objective of this study is to investigate the possibility of using acoustic recording sensor to evaluate the diversity of forest birds at breeding season by comparing and analyzing the recorded data of bird songs and field bird survey data. We conducted the site survey and recording in 186 points in 48 areas of 4 regions of Gombaeryeong and Jochimryeong around Jeombongsan Mountain and Jugryeong and Gochiryeong around Sobaeksan Mountain from May 2nd to 16th in 2013. The analysis of the correlation between the recording result and Bird Community Index based on the field survey showed that the number of bird species, population, and the number of bird songs by recording was significantly correlated to the number of species and population by field survey. Moreover, the number of bird species and the number of bird songs by recording showed a significant correlation to species diversity and species richness but no or low significant correlation to species evenness by the field study (observation and listing in parallel). As a result, it was possible to check species composition and species diversity of bird communities by analyzing acoustic recording data on the field. The acoustic recordings of bird songs in the breeding period were more reliable than the non-breeding period in the correlation of recording result and species diversity and for utilization.