• Title/Summary/Keyword: rice seeds

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Study on Ecological Restoration of Endangered Species in Abandoned Paddy of Korea and Management Plan for its Habitat (한국의 묵논에서 멸종위기식물의 복원생태학적 연구 및 서식지 관리방안)

  • Lee, Soo-In;Lee, Eung-Pill;Hong, Young-Sik;Kim, Eui-Joo;Lee, Seung-Yeon;Park, Jae-Hoon;Jang, Rae-Ha;You, Young-Han
    • Journal of Wetlands Research
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    • v.22 no.2
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    • pp.81-91
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    • 2020
  • As part of method for ecologically utilizing abandoned paddy, potential of the abandoned paddy as a target site for ex-situ conservation of 9 endangered species of different life form was confirmed by considering the nature of rice paddy. In order to create Dum-bung, a component of traditional Korean rice paddy, a part of the abandoned paddy was modified to change the water environment. The seeds, asexual reproductive and sexually reproductive individuals of each endangerd species were transplanted into the abandoned paddy to observe the survival rate and phenological response for 1 year, and then monitored for 2 years. As a result, Hydrophyte 4 taxa (Euryale ferox, Saururus chinensis, Dysophylla yatabeana, Menyanthes trifoliata), Geophyte taxa 1 (Epilobium hirsutum), and Hemicryptophyte taxa 1 (Cicuta virosa) could be introduced into the abandoned paddy. In particular, Euryale ferox, Dysophylla yatabeana, and Menyanthes trifoliata should be introduced into Dum-bung, and Saururus chinensis, Epilobium hirsutum, and Cicuta virosa should be introduced into paddy wetland. Growth of Euryale ferox and Brasenia schreberi was inhibited by herbaceous species, and the growth of Epilobium hirsutum was inhibited by herbivores. Therefore, in order to help efficient settlement of endangered plants introduced in abandoned paddy, it is necessary to remove herbs that inhibit growth and to manage herbivores. In addition, it is necessary to prevent the collapse of paddy bank by planting on the paddy field trees or herbaceous forming vegetation mat. When using abandoned paddy ecologically, it is effective to diversify the moisture environment by creating a Dum-bung to increase biodiversity.

Effect of Medium, Soil, and Irrigation Water Contaminated with Escherichia coli and Bacillus cereus on the Microbiological Safety of Lettuce (Escherichia coli 와 Bacillus cereus에 오염된 상토, 토양 및 관개용수가 상추의 미생물 안전에 미치는 영향)

  • Kim, Se-Ri;Lee, Seo-Hyun;Kim, Won-Il;Kim, Byung-Seok;Kim, Jun-Hwan;Chung, Duck-Hwa;Yun, Jong-Chul;Ryu, Kyoung-Yul
    • Horticultural Science & Technology
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    • v.30 no.4
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    • pp.442-448
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    • 2012
  • Many outbreaks of food-borne illnesses have been associated with the consumption of fresh vegetables and fruits contaminated with food-borne pathogens. Contaminated medium, manure and irrigation water are probable vehicles for the pathogen in many outbreaks. The aim of this study was to determine the potential transfer of Escherichia coli and Bacillus cereus from medium and soil fertilized with contaminated compost or irrigation with contaminated water to the edible parts of lettuce. Moreover, survivals of the two pathogens on lettuce contaminated medium, soil and irrigation water were estimated. Lettuce seeds were planted in medium contaminated with 7.5 log colony forming unit (CFU)/g of E. coli and B. cereus. Seedlings grown in the contaminated medium were transplanted in soil fertilized with contaminated pig manure compost or uncontaminated soil. Contaminated irrigation water with E. coli and B. cereus at 8.0 log CFU/mL was applied only once on the plant by sprinkle irrigation and surface irrigation. Although E. coli and B. cereus in medium and sprouted lettuce after planting seeds were reduced as time passed, these pathogens survived in seedling raising stage for extended periods. The numbers of E. coli and B. cereus in lettuce grown on contaminated soil were detected over 4.0 log CFU/g for 21 days. The numbers of E. coli and B. cereus in lettuce applied by sprinkle irrigation were higher than those of surface irrigation by 5.0 log CFU/g. Our results indicated that contaminated medium, soil and irrigation water can play an important role in the presence of food-borne pathogens on vegetables.

A New Vegetable Soybean Cultivar, 'Sangwon' with Early Maturity and High Yield (풋콩용 조숙 다수성 신품종 '상원')

  • Ko, Jong-Min;Baek, In-Youl;Han, Won-Young;Kim, Hyun-Tae;Oh, Ki-Won;Shin, Sang-Ouk;Park, Keum-Yong;Ha, Tae-Jung;Shin, Doo-Chull;Chung, Myung-Geun;Kang, Sung-Taek;Yun, Hong-Tae;Oh, Young-Jin;Lee, Jong-Hyung;Son, Chang-Ki;Kim, Yong-Deuk
    • Korean Journal of Breeding Science
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    • v.42 no.6
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    • pp.684-689
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    • 2010
  • 'Sangwon', a new cultivar for vegetable soybean, was developed from the cross between 'Keunolkong' and 'Oshimamidori', and was released at the National Institute of Crop Science (NICS) in 2007. The goal to develop a vegetable soybean cultivar with green pod, early maturity, large seed size, high yield, lodging tolerance, and resistance to disease such as soybean mosaic virus (SMV). 'Sangwon' has light green pod, early maturity, large seed, short plant height, and lodging tolerance. 'Sangwon' has determinate growth habit, white flower, gray pubescence, and oval leaf shape. The matured seeds have a yellow seed coat with light brown hilum, and a yellow cotyledon. 'Sangwon' has 5.8 cm fresh pod length, 13.2mm fresh pod width, 69.5 g seed weight per 100 green seeds, 44.0% green seed protein content, and 14.8% green seed oil content. At the regional yield trials (RYT) for vegetable soybean from 2005 to 2007, 'Sangwon' shows strong resistance to soybean mosaic virus (SMV) and tolerance to lodging in fields. Fresh pods of 'Sangwon' were harvested at the beginning of August. In the same tests, fresh pod of 'Sangwon' (10.39ton/ha) yielded 5% higher than 'Hwaeomputkong' (9.90ton/ha).

Studies on the Soil Transmission of CGMMV and Its Control with Crop Rotation (오이녹반모자이크바이러스의 토양전염 생태 및 윤작에 의한 방제)

  • Park, Jin-Woo;Jang, Tae-Ho;Song, Sung-Ho;Choi, Hong-Soo;Ko, Sug-Ju
    • The Korean Journal of Pesticide Science
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    • v.14 no.4
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    • pp.473-477
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    • 2010
  • Cucumber green mottle mosaic virus (CGMMV) is one of major plant viruses infecting cucurbitaceous crops via soil or infected seeds. This study investigate ecology of infection of CGMMV in soil, and control tactics of this virus with soil hygiene and crop rotation. This virus was survival to 50% in soil without host plants for 17 months and had high vitality in debris of infected plant over 1 year. Infection rate of CGMMV was 1.0~3.6% in control soil and 12~36% in soil transplanted with wounded root of watermelon. It showed that wounded root may affect severity of soil infection. Rotation between rice and watermelon caused dramatical reduction from 76.8% in repeated cultivation to 7.3% of progeny infection by CGMMV. Therefore, it is suggested that crop rotation be effective for control of CGMMV.

Development of Proteomics-based Biomarkers for 4 Korean Cultivars of Sorghum Seeds (Sorghum bicolor (L.) Moench) (국내 수수 종자 분석을 위한 프로테오믹스-기반 바이오마커 개발)

  • Kim, Jin Yeong;Lee, Su Ji;Ha, Tae Joung;Park, Ki Do;Lee, Byung Won;Kim, Sang Gon;Kim, Yong Chul;Choi, In Soo;Kim, Sun Tae
    • Korean Journal of Environmental Agriculture
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    • v.32 no.1
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    • pp.48-54
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    • 2013
  • BACKGROUND: Sorghum (Sorghum bicolor (L.) Moench) ranks as the 6th most planted crop in the world behind wheat, rice, maize, soybean, and barley. The objective of this study was to identify bio-marker among sorghum cultivars using proteomics approach such as two-dimensional polyacrylamide gel electrophoresis (2-DE) coupled with mass spectrometry (MS). METHODS AND RESULTS: Proteins were extracted from sorghum seed, and separated by 2-DE. Total 652 spots were detected from 4 different sorghum seed after staining of 2-DE with colloidal Coomassie brilliant blue (CBB). Among them, 8 spots were differentially expressed and were identified using MALDI-TOF/TOF mass spectrometry. They were involved in RNA metabolism (spot1, spot 4), heat shock proteins (HSPs, spot 2), storage proteins (spot 3, spot 5, and spot 6), and redox related proteins (spot 8). Eight of these proteins were highly up-regulated in Whinchalsusu (WCS). The HSPs, Cupin family protein, and Globulin were specifically accumulated in WCS. The DEAD-box helicase was expressed in 3 cultivars except for WCS. Ribonuclease T2 and aldo-keto reductase were only expressed in 3 cultivars except for Daepung-susu (DPS). CONCLUSION(S): Functions of identified proteins were mainly involved in RNA metabolism, heat shock protein (HSP), and redox related protein. Thus, they may provide new insight into a better understanding of the charactreization between the cultivars of sorghum.

Identification of Physiologically Active Compounds from Purslane(Portulaca oleracea L.) (쇠비름(Portulaca oleracea L.)에 함유(含有)된 생리활성물질(生理活性物質) 탐색(探索))

  • Park, J.S.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.8 no.2
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    • pp.169-175
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    • 1988
  • This experiment was conducted to determine the presence of allelopathic substance in Portulaca oleracea L. Water and methanol extract from P. oleracea markedly inhibited the germination of lettuce, rice, raddish etc., indicating the presence of biological substances. The biochemical substances such as ferulic, p-coumaric, salicylic, vanillic, p-hydroxybenzoic acid etc., belonging to phenolic compounds were detected in a large amount, which may be responsible for exhibiting inhibitory effects. Various phenolic compounds were detected from different samples such as freshly, dried plants, seeds and callus. The highest amount of tannic and gallic acids were detected in dried samples of P. oleracea, the highest grantity of salicylic and vanillic acids in fresh samples, the largest amount of ferulic acid in seed sample, the highest amount of p-hydroxybenzoic and p-chlorobenzoic acids in callus. Linolenic acid was presented in amount of 9.203 mg/g in dried plant of P. oleracea as one of the major fatty acids and oxalic acid presented 27.941 mg/g as one of the major organic acids. These compounds seemed to be related to inhibitory effect of P. oleracea which needs further study.

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Studies on the High Protein Mutants of Rice (수도 고단백 돌연변이계통에 관한 연구)

  • Chang-Yawl Harn;J. L. Won
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.20
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    • pp.63-68
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    • 1975
  • Several high protein mutant lines(M4 plant generation, 1974) obtained from X-ray irradiated Jinheung variety were examined at three different locations for their agronomic characters, protein and grain yields. On the other hand, high protein-short culmed-early maturity mutant line No. 398 (M$_{10}$ plant generation, 1974) induced from Hokwang was crossed back to its mother to investigate the gene(s) controlling protein and its pleiotropic relation to other mutated characters. Although variation of protein percent of mutant lines from Jinheung was comparatively large depending on year and location, most of the high protein mutant lines had higher protein yield per unit area than the mother variety and their grain yields were equal to or better than the mother, being resistant to both leaf and neck blast. They were several days earlier-maturing and had shorter-culm except one mutant line. The culm length and heading date of F$_1$ between high protein mutant 398 and its mother Hokwang were intermediate. Accurate assessment of segregation of culm length and heading date in F$_2$ generation and protein percent in F$_3$ seeds will be conducted in 1975.

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Comparative Performance of Broilers Fed Diets Containing Processed Meals of BT, Parental Non-BT Line or Commercial Cotton Seeds

  • Elangovan, A.V.;Mandal, A.B.;Johri, T.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.1
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    • pp.57-62
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    • 2003
  • An effort was made to assess comparative production performance in broiler chickens fed diets containing solvent extracted cottonseed meal (CSM) processed from BT and Parental Non-BT lines. Processed meal of national check and commercial produce cottonseeds were also used for comparison. The free gossypol contents were 0.02, 0.02, 0.44 and 0.03% in meals of BT, Parental Non-BT, national check and commercial produce cottonseeds, respectively. Day-old broiler chicks (n=243) were divided to 27 groups of 9 each. Nine dietary treatments (iso-nitrogenous, 23% CP and iso-caloric, 2,800 kcal ME/kg) were formulated viz., D1 (control, soybean meal-SBM based), D2 and D3 (BT CSM at 10% of diet with and without additional iron), D4 and D5 (non-BT CSM with and without additional iron), D6 and D7 (national check CSM with or without additional iron), and D8 and D9 (commercial produce CSM with or without additional iron at 2 ppm for every 1 ppm of free gossypol, respectively). Each dietary treatment was offered to three replicated groups up to 6 weeks of age. At the end of 6 weeks of age, 10 birds were taken out randomly from each treatment and were sacrificed to study carcass traits, organs' yield and histo-pathological changes in vital organs. The broiler chickens received CSM processed from BT (D2, 1,753 g and D3, 1,638 g) and Parental Non-BT (D4, 1,653 g and D5, 1,687 g) with or without additional Fe grew at same rate as observed in soybean meal (solvent ext.) based diet (D1, 1,676 g). The feed intake and feed conversion efficiency (feed: gain) in these dietary treatments (BT, non-BT line based diets) also did not differ significantly (p>0.05) from control diet. Similar observation was also observed in dietary treatments (D8 and D9) containing solvent extracted cottonseed meal of commercial produce origin. However, a decrease (p<0.05) in body weight gain and feed intake was observed in D6 containing national check CSM with high gossypol content. Addition of Fe in the diet (D7) improved (p<0.05) feed intake and weight gain but not to the extent as observed in diets containing BT, parental non-BT, and commercial produce CSM or control. However, any type of CSM did not affect feed conversion efficiency when fed with or without additional iron. The carcass characteristics in terms of dressing percentage, liver weight and heart weight was not significantly (p>0.05) different between the treatments. The eviscerated yields emanated from diets containing either BT, non-BT or commercial produce were statistically similar to control. However, eviscerated yield of broilers fed national check CSM with or without iron supplementation was lower (p<0.05) than BT cotton with Fe supplementation and commercial produce CSM. The study envisaged that BT, parental non-BT and commercial produce solvent extracted cottonseed meal can be included at 10% in soybean meal based broiler diet replacing soybean meal and rice bran without additional iron.

2 Cropping systems using field crops in unheated plastic house at paddy field

  • Shin, Jung-Ho;Moon, Jin-Young;Song, Jae-Ki;Choi, Yong-Jo;Hong, Kwang-Pyo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.279-279
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    • 2017
  • In Korea, the single span unheated plastic house cultivated crops from autumn to spring of the following year, removed the plastic film and frame, cultivated rice, set up a plastic house again and cultivated crops. The crops in the greenhouse are utilized mainly for the production of leaf vegetables such as lettuce, leek, and fruit vegetables such as strawberry, watermelon, oriental melon, etc. and raising high income. Because, the production of these crops has characteristics requiring a lot of labor and it is difficult to produce horticultural crops at unheated plastic houses as the rural population ages. Therefore, we conducted a test to develop a crop planting system to cultivate crops in single span unheated plastic houses, although the utilization of labor is less than that of horticultural crops. The prior cropping cultivated three cultivars of sweet potatoes early, the second produced cultivated sweet potatoes, corn and soybeans. In the cultivation of the previous cropping, the sweet potatoes were harvested on the 113th day after planting on March 30th, the yield was 822 kg/10a for Pungwonmi, 1,377 kg/10a for Jinhongmi, 1,483 kg/10a for the Dahomi. Because of differences, the yield of Pungwonmi cultivar was less than that other cultivars and the yield of open field cultivations, we will expect further research. In the cultivation of the succeeding crops sweet potatoes were planted on July 27 and harvested 110 days later and investigated. The product yield of Pungwonmi cultivar was 1,024 kg/10a, and the Jinhongmi, Dahomi cultivars were not at economic level for sale and were necessary to review. In succeeding-crops, corn tested the Ilmichal cultivar, seeded on 27th July, harvested on October 11th. The day of silking was 45 days after sowing, the yield was 1,156 kg/10a, the goods rate was 100% level. The beans in the succeeding cultivation crop were sowed on 27th July, the early maturing of the varieties coming to Hwangeumol and Saeol cultivar, on 17th October, the late maturing soybean Daewonkong cultivar were harvested on October 21st. The yield of early maturing two cultivars was 214 kg/10a, Daewonkong was 257 kg/10a, and 100 seeds weight which were more than the early maturing beans were also heavy. When calculating these incomes price-wise according to the harvest time, we were able to consider the income in the order of corn, sweet potato and soybean from the second term crop. Various studies such as varieties, mulching method, moisture management, control environment management, etc. are considered necessary to develop cropping systems with sweet potato and field crops in future unheated plastic house.

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Analysis of germinating seed stage expressed sequence tags in Oryza sativa L. (벼 발아종자 발현유전자의 발현특성분석)

  • Yoon, Ung-Han;Lee, Gang-Seob;Kim, Chang-Kug;Lee, Jung-Sook;Hahn, Jang-Ho;Yun, Doh-Won;Ji, Hyeon-So;Lee, Tae-Ho;Lee, Jeong-Hwa;Park, Sung-Han;Kim, Gun-Wook;Seo, Mi-Suk;Kim, Yong-Hwan
    • Journal of Plant Biotechnology
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    • v.36 no.3
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    • pp.281-288
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    • 2009
  • Seed germination is the important stage to express many genes for regulation of energy metabolism, starch degradation and cell division from seed dormancy state. For the functional analysis of seed germination mechanisms, we were analyzed the rice cDNA clones (Oryzasativa cultivar Ilpum) obtained from seed imbibition during 48 hours. Total number of 18,101 Expressed Sequence Tags (ESTs) were clustered using SeqMan program. Among them, 8,836 clones were identified as unique clones. We identified the chitinase gene specifically expressed in seed germination and amylase gene involved to starch degradation from the full length cDNA analysis, and several genes were registered to NCBI GeneBank. To analyzed the commonly expressed genes between inmature seed and germinated seed, 25,66 inmature ESTs and 18,101 germinated ESTs were clustered using SeqMan program and identified 2,514 clones as commonly expressed unigene. Among them, alpha-glubulin and alcohol dehydrogenase I were supposed to LEA genes only expressed in the immature and germinated seed stages. For the clustering of orthologous group genes, we further analyzed the 8,836 EST clones from germinating seeds using NCBI clusters of orthologous groups database. Among the clones, 5,076 clones were categorized into information storage and processing, cellular processes and signaling, metabolism and poorly characterized genes, proportioning 783 (14.29%), 1,484 (27%), 1,363 (24.8%) and 1,869 (34%) clones to the previous four categories, respectively.