• Korean Journal of Weed Science
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• v.31 no.3
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• pp.308-312
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• 2011

A new paddy field herbicide, metazosulfuron granule is an herbicide for controlling annual and perennial weeds occurring in machinery transplanting paddy field. It is recommended to apply $30kg\;ha^{-1}$ 15 days after transplanting. As a result of preliminary tests, it could control seven annual species such as Echinochloa crus-galli, Bidens tripartita, Ludwigia prostrata, Monochoria vaginalis var. plantaginea, etc. and four perennial species including Cyperus serotinus, Sagittaria trifolia, Eleocharis kuroguwai, and Scirpus juncoides. Monochoria vaginalis var. plantaginea, S. trifolia and S. juncoides resistant to sulfonylurea- herbicides were also controlled. Because application timing of the herbicide is wide, it is very efficient to control the spraying timing by users. Phytotoxicity of rice cultivars was not observed, and the impact on the environment is very limited.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.211-214
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• 2008

Haploid system by anther culture allows the development of homozygous lines when doubled. The response of anther culture to Basta (glufosinate) resistance was investigated on transgenic plants (cv. Anjungbyeo) in order to identify inheritance of bar gene associated with Basta. Most of the regenerated transgenic plants were sterile, and only a few plants produced viable seeds ($A_1$) in the greenhouse. The bar gene was analysis by PCR in basta resistant transgenic plant ($TA_0$). The transgenic seeds ($A_1$) were significantly germinated in Basta solution compared with non-transformed seeds. As a result of anther culture, in regenerated haploid plants, segregation ratio was 1:1 in five of eight cross combinations. In diploid plants, segregation ratio was 1:1 in seven of eight cross combinations. Although there was some differences in the cross combinations, most of the combinations had 1:1 segregation ratio which supports the theory. The difference may be a result of the small sample size or the difference of anther culture response caused by genotypic difference. Hence, when many cross combinations were anther-cultured the results would support the theory.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.111-111
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• 2003

Several fungal isolates were isolated from diseased monochoria(Monochoria vaginalis, weed of paddy field), which has an resistance to sulfonyl urea(S.U.) herbicide, and were evaluated in the laboratory and greenhouse as potential mycoherbicide. Eight fungi, Alternaria sp., Colletotrichum sp., Curvularia sp., Paenicillium sp and etc. were observed in the isolates. Pathogenicity testing were done on the monochorias in the greenhouse. Monochorias were inoculated with suspensions containing conidia of each isolate at the rates of 1.0 ${\times}$ 10$\^$5/ conidia/ml and 0.1％ Tween 80 with hand-gun sprayer. Curvularia sp. and an unidentified fungal isolate caused 90-95％ mortality on the monochorias 15∼20 days after inoculation. However the other isolates induced slight symptom of disease on the monochorias. In the early stage of disease development sun-burn appearance was shown at the infected site and the last infected leaves and stems were withered to death. Subsequently the pathogenicity on the rice was evaluated with above two effective isolates. From the test an unidentified isolate showed pathogenicity on the rice but Curvularia sp., named as DBB2003, didn't. Now the mass production and formulation using Curvularia sp. DBB2003 are in progress and the field test will be followed. Combination product with Curvularia sp. DBB2003 and chemical herbicide will be more effect to control the monochoria resisted on S.U. herbicide and need to be further tested.

• Journal of Plant Biotechnology
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• v.44 no.4
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• pp.472-477
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• 2017

Barnyardgrass (Echinochloa crus-galli) is one of the worst weeds in rice (Oryza sativa), but there are few reports about the genetic diversity and herbicide resistance of barnyardgrass in Vietnam. In this study, we used random amplified polymorphic DNA (RAPD) analysis and greenhouse testing to study the genetic diversity and quinclorac resistance levels of 15 Echinochloa crus-galli populations in the Mekong Delta, Vietnam, and the state of Arkansas, U.S. The quinclorac resistance of Echinochloa crus-galli populations in Vietnam was confirmed; 9 populations were resistant to quinclorac with R/S ratios ranging from 1.9 to 6.3. Six oligonucleotide primers produced a total of 55 repeatable bands of which 46 were polymorphic (83.3% average) among the 15 populations. Genetic distance was calculated, and cluster analysis separated the 15 populations into 2 main clusters with the genetic distances within the clusters ranging from 0.09 to 0.39. The two main clusters were divided into 7 subclusters, and the quinclorac resistant and susceptible populations were located randomly within each subcluster. Six out of 13 weed populations from Vietnam belonged to one cluster and a single Echinochloa species. The remaining 7 populations were identified as potentially different species in the Echinochloa genus. Nine Echinochloa populations from Vietnam were tested and identified as quinclorac resistant. The connection between quinclorac resistance levels and weed groups defined by RAPD analysis in the study is unclear; the quinclorac resistance of each resistant population could have evolved individually, regardless of differences in genetic diversity and location of the sampled populations.

• Korean Journal of Weed Science
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• v.2 no.2
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• pp.146-151
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• 1982

Herbicide mixtures, butachlor + naproanilide, CG113 + naproanilide and benthiocarb + naproanilide were tested in order to control Sagittaria pygmaea Miq., one of the most serious perennial weeds in paddy field of Honam area. 92% of tuber of Sagittaria pygmaea Miq. was distributed within 9cm from the soil surface and number of emerged Sagittaria pygmaea Miq. increased until 40 days after rice transplanting but decreased thereafter. Number and dry weight of survived Sagittaria pygmaea Miq. were much less in butachlor + naproanilide, CG 113 + naproanilide and benthiocarb + naproanilide treatments than perfluidone and it was controlled by up to 95% by mixture treatments. Number of rhizome of Sagittaria pygmaea Miq. was decreased by all herbicide treatments and especially mixture treatment significantly reduced rhizome formation compared to perfluidone and 2.4 -D treatments. CG113 + naproanilide treatment caused phytotoxicity that tip of leaf sticked to leaf sheath curvedly in Indica ${\times}$ Japonica rice, Iri 358, but it was recovered within 10 days after herbicide treatment.

• Weed & Turfgrass Science
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• v.6 no.4
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• pp.292-305
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• 2017

This paper provides the current status of weed science and prospects for the development of weed science based on the research trends presented at the 26th Asian Pacific Weed Science Conference in 2017. Approximately 458 researchers from 25 countries, including Korea, participated in the conference and presented 325 papers in 20 research areas. Major research topics were herbicide resistance, herbicide use, herbicide development, weed ecology, allelopathy, weed management, and exotic weeds. Particularly, there were many presentations and interesting to researchers about the development and use of new herbicides, such as florpyauxifen-benzyl ester, triafamone, fenquinotrione, and tolpyralate. Development of new herbicide formulations and spray methods were suggested as a solution for the population decline in rural area and low labor quality especially in Asia and Pacific regions. In future weedy rice and exotic weeds will be a serious problem in this area so we need to cooperate to make good technical and practical solutions.

• Journal of Plant Biotechnology
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• v.33 no.2
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• pp.75-84
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• 2006

Heteroplasmic rice plastid transformant was generated using suspension cells as bombardment materials. PCR analyses confirmed incorporation of aadA and cp4-epsps genes into the rice plastid genome by homologous recombination events via the flanking sequences of the trnI and trnA. Transplastomic calli were actively proliferated when cultured on AAM2 medium supplemented with various concentrations (500-3000 mg/L) of streptomycin in dark condition, and transplastomic suspension cells showed resistance to nonselective herbicide, glyphosate. Through 'agarose pie selection' method, heteroplastomic calli, containing considerably high level of transplastome and expressing the CP4 EPSPS protein, were obtained. They were further regenerated to green shoots with healthy roots.

• Korean Journal of Environmental Agriculture
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• v.28 no.2
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• pp.139-145
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• 2009

Weed control is one of the important and difficult practices in environment-sound agriculture. This study was conducted to establish environment-sound weed control in paddy fields using rice bran pellet. Application of rice bran pellet at a rate of 1${\sim}$3 Mg $ha^{-1}$ reduced weed occurrence by 46${\sim}$69% without using any chemical herbicide. However, rice bran application at a rate of greater than 2 Mg $ha^{-1}$ increased protein content in milled rice and decreased palatability value. Therefore, fertilization rate before transplanting needs to be reduced from $N-P_2O_5-K_2O$ = 55-45-40 kg $ha^{-1}$ to 28-23-20 kg $ha^{-1}$, in order to decrease protein content and increase palatability value without decreasing rice yield. Pyroligneous acid and brown rice vinegar had a relatively weak activity to inhibit weed emergence compared with rice bran. When butachlor herbicide was applied at a rate of 0.75 kg $ha^{-1}$, 50% of recommended rate, co-application of rice bran pellet at a rate of 1 Mg $ha^{-1}$ enhanced weed control value from 63% to 75%.

• Proceedings of the Korean Society of Crop Science Conference
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• 1998.10a
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• pp.210-232
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• 1998

Tong-il, the high-yielding rice variety bred on early 1970, effected a turning point in modern rice production in Korea. As rice production reached the highest record yield in 1978 with HYV, Korea achieved self-sufficiency in domestic supply of rice (or the first time in (her own) history, HYV required high input of fertilizers and pesticides for proving its yielding ability and needed new techniques such as early nursery-planting to prevent chilling damage. But, farm economy did not follow the successful achievement of rice production because of increased farming cost and inflation.'Tong-il variety has been replaced by new high-yielding Japonica varieties from 1980 when record-low-temperature during summer months had persisted. Also, the cooked rice of Tong-il variety did not agree with the appetite of Korean people. Though the hectarage of Tong-il rice did reduce, farmers applied the same high-input cultural techniques for new Japonica cultivars as did for Tong-il variety. Heavy application of nitrogen fertilizer contaminated surface and ground water with nitrate ions while phosphorous fertilizer was blamed for algae pollution. Frequent spray of pesticide and herbicide reduced significantly the biotic population in paddy ecosystems including insects and soil microorganisms. The new technologies of the 21st century must be directed to produce safe food, to save natural resources, and to preserve a clean environment for human welfare. We need low-input sustainable farming techniques to provide high-yielding crops and to preserve a healthy ecosystem.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.556-561
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• 2010

Lactoferrin is an 80-kDa iron-binding glycoprotein that is found in high concentrations in human milk. Human lactoferrin (hLF) has several beneficial biological activities including immune system modulation and antimicrobial activity. In the present study, we devolved a method of hLF expression through introducing the hLF gene construct into Oriza sativa cv. Nakdong using the Agrobacterium-mediated transformation system. The expression of the hLF gene under the control of the rice glutelin promoter was detected in the seeds of transgenic rice plants. Transformed rice plants were selected on media containing herbicide(DL-phosphinothricin) and the integration of hLF cDNA was confirmed by Southern blot analysis. The expression of the full length hLF protein from the grains of transgenic rice plants was verified by Western blot analysis. The lactoferrin expression levels in the transformed rice grains determined by enzyme-linked immunosorbant assay accounted for approximately 1.5% of total soluble protein. Taken together, these data indicate that rice grains expressing hLF can be directly incorporated into infant formula and baby food.