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http://dx.doi.org/10.5010/JPB.2006.33.2.075

Expression of the Glyphosate Resistant Gene, cp4-epsps, through Plastid Transformation in Rice (Oryza sativa L.)  

Kang, Kyung-Su (School of Agricultural Biotechnology, Seoul National University)
Kim, Min-Kyun (School of Agricultural Biotechnology, Seoul National University)
Publication Information
Journal of Plant Biotechnology / v.33, no.2, 2006 , pp. 75-84 More about this Journal
Abstract
Heteroplasmic rice plastid transformant was generated using suspension cells as bombardment materials. PCR analyses confirmed incorporation of aadA and cp4-epsps genes into the rice plastid genome by homologous recombination events via the flanking sequences of the trnI and trnA. Transplastomic calli were actively proliferated when cultured on AAM2 medium supplemented with various concentrations (500-3000 mg/L) of streptomycin in dark condition, and transplastomic suspension cells showed resistance to nonselective herbicide, glyphosate. Through 'agarose pie selection' method, heteroplastomic calli, containing considerably high level of transplastome and expressing the CP4 EPSPS protein, were obtained. They were further regenerated to green shoots with healthy roots.
Keywords
Glyphosate; particle bombardment; rice plastid transformation; streptomycin; suspension culture;
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Times Cited By KSCI : 1  (Citation Analysis)
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