• Title/Summary/Keyword: restriction map.

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CORESTRICTION MAP ON BRAUER SUBGROUPS

  • CHOI, EUN-MI
    • Communications of the Korean Mathematical Society
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    • v.20 no.1
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    • pp.35-49
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    • 2005
  • For an extension field K of k, a restriction homomorphism on Brauer k-group B(k) maps Brauer k-algebras to Brauer K- algebras by tensor product. A purpose of this work is to study the restriction map that sends radical (Schur) k-algebras to radical (Schur) K-algebras. And we ask an analogous question with respect to corestriction map on Brauer group B(K) that whether the corestriction map sends radical K-algebras to radical k-algebras.

Restriction Map of the R Plasmid pKU10 in Pseudomonas putida (Pseudomonas putida에 내재하는 Plasmid pKU10의 제한지도)

  • 전성희;임영복;심웅섭;이영록
    • Korean Journal of Microbiology
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    • v.29 no.4
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    • pp.226-229
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    • 1991
  • In our laboratory a R plasmid pKU10 was isolated from Pseudomonas and its characteristics were investigated. In this study, as a basic work to improve its utility as a cloning vehicle, restriction patterns of pKU10 were analyzed for other various restriction enzymes in addition to restriction evdonucleases previously examined. As a result, pKU10 DNA has two cleavage sites for ClaI and HpaI, and three sites for AvaI. The restriction map of pKU10 was supplemented with AvaI, ClaI, and HpaI. From the result of this experiment, the usefulness of PKU10 as a cloning vector in Pseudomonas will be enhanced by constructions of mini-plasmid or hybrid plasmids.

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Restriction and transcription maps of mitochondrial DNA of trimorphomyces papilionaceus

  • Jeoung, Won-Jin;Hong, Soon-Gyu;Won, Kang-Young;Jung, Hack-Sung
    • Journal of Microbiology
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    • v.33 no.2
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    • pp.149-153
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    • 1995
  • Mitochondrial DNA has been isolated from Trimorphomyces papilionaceus. By analyzing DNA fragments digested by restriction enzymes, a restriction site map has been constructured. The mtDNA of T. papilionaceus amounts to 48.5 kb in size and is circular in structure. Entire mitochondrial DNA was cloned in E coli plasmids and Northern blot hybridization was done using cloned and subcloned DNAs as probes. Based on hybridization results of mitochondrial RNA transcripts, a transcription map was prepared.

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Characterization of Tetracycline Resistance Plesmid of Multidrug-resistant Staphylococcus aureus (다제내성 황색포도상구균이 가지고 있는 테트라사이클린 내성 플라스미드의 동정)

  • 이대운;문경호
    • YAKHAK HOEJI
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    • v.39 no.1
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    • pp.6-9
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    • 1995
  • The clinical isolate Staphylococcus aureus SA2 had four kinds of plasmids and was resistant to ampicillin, chloramphenicol, clindamycin, erythromycin, gentamicin, kanamycin, methicillin, streptomycin, tetracycline and tobramycin. Transformation experiment demonstrated that 4.44 kb plasmid(pKH6) encoded resistance to tetracycline. The cleavage map of pKH6 was determined by restriction enzyme mapping techniques. The cleavage map is given for EcoRV, HindIII, HpaI, HpaII, KpnI and Xbal. Restriction endonucleases BamHl, BglI, BGIII, BstEII, EcoRI, HaellI, PstI, PvuII, SalI, Smal, and Xhol have no site on this plasmid. The restriction map revealed extensive structural homology between pKH6 and pT181.

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Tests of Land Boundary Using Orthoimages (정사영상을 이용한 토지경계설정 실험)

  • Lee, Young-Jin;Ryu, Su-Hyun;Kim, Ho-Young;Park, Jung-Hwan
    • Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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    • 2010.04a
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    • pp.289-290
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    • 2010
  • Orthoimage, digital map, cadastral map, and areas and district were converted and overlaid so as to apply legal restrictions on boundaries set by a land object concept restriction line and to analyze boundaries formed by overlaying areas and districts and characteristics of restrictions. With the restriction lines made on the basis of orthoimage.

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Rapid plasmid mapping computer program (Plasmid의 제한효소 지도 작성을 위한 콤퓨터 프로그램)

  • 이동훈;김영준;이승택;강현삼
    • Korean Journal of Microbiology
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    • v.24 no.1
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    • pp.12-17
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    • 1986
  • A new computer algorithm is described to order the restriction fragments of plasmid DNA which has been cleaved with several restriction endonucleases in single or double digestions rapidly with realistic error rates. The permutation and high weight on small fragments methods construct all logical circular map solutions. The program is written in Apple BASIC and run on an Apple II plus microcomputer with 64K memory. Several examples are presented which indicate the high efficiency of the profram in construction possible restriction map for YEp24.

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Characterization of Tetracycline Resistant Plasmid in Staphylococcus aureus by Restriction Enzyme Mapping (황색포도상구균에서 테트라사이클린 내성을 나타내는 플라스미드의 동정)

  • Kim, Ki-Hyun;Kim, Jong-Myung;Moon, Kyung-Ho
    • YAKHAK HOEJI
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    • v.36 no.3
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    • pp.255-258
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    • 1992
  • The clinical isolate Staphylococcus aureus SA8 was resistant to tetracycline(Tc) and harboured a plasmid pKH1(24.82 kb). pKH1 was shown by curing and by transformation to specify resistance to Tc. The cleavage map of a pKH1 was determined by restricction enzyme mapping techniques. Cleavage map is given for BglII, EcoRI, HpaII, PvuII and SalI. Restriction endonuclease BamHI, BglI, BstEII, HpaI, PstI, and XhoI have no sites on this plasmid. HaeIII, XbaI, and HindIII have 5, 6, 14 sites, respectively.

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Characterization of Chloramphenicol Resistant Plasmid of Multidrug-resistant Staphylococcus aureus (다제내성 황색포도상구균이 가지고 있는 클로람페니콜 내성 플라스미드의 동정)

  • 이대운;문경호
    • YAKHAK HOEJI
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    • v.37 no.6
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    • pp.621-624
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    • 1993
  • The clirical isolate Staphylococcus aureus SA2 had four kinds of plasmids and was resistant to ampicillin, chloroamphenicol, clindamycin. erythromycin, gentamicin, kanamycin, methicillin, streptomycin, tetracycline and tobramycin. Transformation experiment demonstrated that 4.14kb plasmid(pKH7) encoded resistance to chloramphenicol. The cleavage map of pKH7 was determined by restriction enzyme mapping techniques. The cleavage map is given for BstEll, Hindlll, Hpall, and Xbal. The above restriction endonucleases have a single site, but nucleases BamHl, Bgll, BglII, EcoRl, EcoRV, HaeIII, Hpal, Kpnl, Pstl, PvnII, Sall, Smal, and XhoI have no site on this plasmid.

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Restriction endonuclease mapping of the plasmid pTi12 from agrobacterium tumefaciens (Agrobacterium tumefaciens KU-12 균주에서 분리한 플라스미드 pTi 12의 제한효소 지도)

  • 이용욱;손정훈;심웅섭
    • Korean Journal of Microbiology
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    • v.25 no.3
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    • pp.173-179
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    • 1987
  • Ti plasmids were isolated from three strains of Agrobacterium tumefaciens in Korea and their types and molecular weights were determined. All of these are octopine-type and their molecular weights are 44Kb (pTi 12), 180Kb (pTi 14) and 172Kb (pti 49), respectively. In order to construct physical map of pTi 12, pTi 12 was digested with restriction endonucleases Sma I and Hind III. Sma I degestion of pTi 12 produce 8 fragments and Hind III produced 10 fragments. Physical arrangements of these fragments was determined by Southern hybridization techniques.

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Circular Permutation of the DNA Genome of Temperate Bacteriophage $\PhiFC1$ from Enterococcus faecalis KBL 703

  • Kim, Young-Woo;Jang, Se-Hwan;Hong, Bum-Shik;Lim, Wang-Jin;Kim, Chan-Wha;Sung, Ha-Chin;Chang, Hyo-Ihl
    • Journal of Microbiology and Biotechnology
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    • v.9 no.4
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    • pp.457-463
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    • 1999
  • The physical map of bacteriophage $\PhiFC1$ DNA was constructed with the restriction endonucleases SalI, BamHI, EcoRI, XbaI, and AvaI. The 40.5-kb DNA restriction map is shown to be circularly permuted representing the headful packaging mechanism of the phage. The DNA restriction fragments containing the packaging initiation site(pac) was localized on the restriction map and the nucleotide sequences of the region were analyzed. Four open reading frames (ORFs), following one another with the same orientation, were found at the region. The 2nd ORF (ORF-ts) has significant amino acid sequence homologies to the previously known terminase small subunits of other bacteriophages. The putative terminase small subunit gene has a presumptive NTP-hydrolysis motif and a helix-turn-helix motif. The cleavage site for the first round of packaging was found to be located at the coding sequence of the putative terminase small subunit gene. The fourth ORF, even if partially sequenced, has a good amino acid sequence homology to the portal vertex proteins of other bacteriophages representing the evolutionarily conserved arrangements of genes near the pac site of this bacteriophage, $\PhiFC1$.

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