• Environmental Mutagens and Carcinogens
• /
• v.22 no.3
• /
• pp.199-204
• /
• 2002

It has been known that Ganoderma lucidum and Lentinus edodes have anticancer activity and immune enhancing activity. These two mushrooms were grown in liquid culture and harvested. From these mycelia, DNA was isolated and EtBr-CsCl density gradient ultracentrifugation was performed to purify it further. Then mitochondrial DNA was isolated by bisbenzimide-CsCl density ultracentrifugaton. Mitochondrial DNA of Ganoderma lucidum was digested by restriction enzymes, EcoR I, Hind Ⅲ, and Pst I, then electrophoresed. It showed 12, 22, 4 fragments. Mitochondrial DNA of Lentinus edodes was digested by EcoR I. Electric pattern showed 6 fragments. 4 fragments had appeared by Pst 1 digested mitochondrial DNA. Hind ill couldn't digest mitochondrial DNA of Lentinus edodes. Mitochondrial DNA of fusants was isolated to compare to those of parents. The results showed that fusant P₂S₄has new, recombined mitochondrial DNA. But P₂S₄had the same DNA that Ganoderma lucidum had.

• Korean Journal of Veterinary Service
• /
• v.31 no.3
• /
• pp.369-374
• /
• 2008

The objective of this study was to differentiate the beef species between Hanwoo and Holstein from a total of 1,081 beef samples using PCR-RFLP of MC1R gene. When a PCR product of 403 bp specific band amplified from bovine MC1R gene sequence was digested with restriction enzyme MspA1I, Hanwoo type showed 2 bands, 220 bp and 183 bp size bands. Holstein type, however, showed three bands, 220 bp, 138 bp and 45 bp size band, respectively. The results of the differential test for beef species were as following; 7 samples (0.64%) were determined to Holstein type, of which 4 were submitted from administrative authorities, other 3 from self-collection planing, and none from civilian clients including school.

• International Commerce and Information Review
• /
• v.11 no.1
• /
• pp.379-403
• /
• 2009

The Purpose of this study is to research the problems of trade restriction for an environment protection. Environmental regulation relate to trade are Convention on International Trade in Endangered Species of Wild Fauna & Flora, Montreal Protocol on Substances that Deplete the Ozone Layer, Kyoto Protocol to the UN Framework Convention on Climate Change, Basel Convention on the Control of Transboundary Movements of Hazardous Wastes & Their Disposal, Cartagena Protocol on Biosafty and WTO Agreement. Regulatory action for environmental protection has economics instrument, command & control, liablity, damage compensation, voluntary agreement. In the case of our country, impact of regulatory action for environmental protection is low. Because is recognized position of developing country yet. For in the balance rules of trade and enviroment, First must satisfy WTO's basic principles and principle of quantitative restrictions prohibition, Second, operation of protection action must reasonable and objective standards Third, must satisfy GATT article 20 (b) clause and (g) protestation each essential factor To grow for environment advanced country, we should do i) using of FTA ii) international cooperation strengthening for developing country position iii) construction of environment information network

• YAKHAK HOEJI
• /
• v.37 no.5
• /
• pp.504-510
• /
• 1993

Human insulin gene is consisted of the polymorphic region with the repeating units, the regulatory sequence, the structural gene including the intervening sequence, and 3'-flanking region. The polymerase chain reaction, which amplifies the target DNA between two specific primers, has been performed for the amplification of human insulin gene and simple one-step cloning of it into Escherichia coli. Out of 1727 nuceotides compared, only 4 sites were variable: 5'-regulatory region(G2101$\rightarrow$AGG); IVS I(T2401$\rightarrow$A); Exon II(C2411 deletion); IVS II(A2740 dejection). The variations at the G2101 and T2401 were the same as those found in one American allele. The other two variations were observed only in the specific Korean allele. And, the enzyme digestion patterns among normal, insulin dependent diabetes mellitus, and non-insulin dependent diabetes mellitus were the same. On the other hand, PCR method showed the possibility of the quickaccess for the polymorphic region in terms of the restriction fragment length of polymorphism.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.9 no.6
• /
• pp.85-93
• /
• 2001

Minimized canister flow restriction and maximized flow uniformity are desired to maximize a purge capability. With the impending ORVR(On Board Refueling Vapor Recovery) systems, the reduction of restriction and increase of flow uniformity in a carbon canister becomes even more critical to meet the stringent regulation. In this study, three-dimensional numerical simulations have been performed to investigate the three-dimensional internal flow patterns in a carbon canister during purge. The effects of the declined angle of the purge pipe and the number of partitions on the pressure drop and purge efficiency in a carbon packed bed are examined. Results show that the purge efficiency and space velocity distribution are affected in the upstream region of 40% of total canister bed by porosity of carbon granule and angle of purge pipe. It is also found that the purge efficiency decreases with increasing the number of partitions.

• Kyungpook Mathematical Journal
• /
• v.55 no.2
• /
• pp.449-472
• /
• 2015

Let X be a nonempty set and $\mathcal{F}$(X) be the set of nonempty finite subsets of X. The paper deals with the extended metrics ${\tau}:\mathcal{F}(X){\rightarrow}\mathbb{R}$ recently introduced by Peter Balk. Balk's metrics and their restriction to the family of sets A with ${\mid}A{\mid}{\leqslant}n$ make possible to consider "distance functions" with n variables and related them quantities. In particular, we study such type generalized diameters $diam_{{\tau}^n}$ and find conditions under which $B{\mapsto}diam_{{\tau}^n}B$ is a Balk's metric. We prove the necessary and sufficient conditions under which the restriction ${\tau}$ to the set of $A{\in}\mathcal{F}(X)$ with ${\mid}A{\mid}{\leqslant}3$ is a symmetric G-metric. An infinitesimal analog for extended by Balk metrics is constructed.

• Microbiology and Biotechnology Letters
• /
• v.21 no.6
• /
• pp.534-541
• /
• 1993

The relationship between Schwanniomyces occidentalis CBS 2863 (formerly castellii) and CBS 1153 (formerly alluvius), and their variety persoonii was examined at alpha-amylase gene level. Using Sch. occidentalis alpha-amylase gene as probe, Sch. occidentalis alpha-amylase gene homologues were obtained from Sch. occidentalis CBS 1153 and Sch. occidentalis var. persoonii. The restriction analysis of these homologues showed that the restriction enzyme sites between Sch. occidentalis CBS 2863 and CBS 1153 was identical but different between these strains and Sch. occidentalis var. persoonii.

• Journal of Information Technology Applications and Management
• /
• v.9 no.2
• /
• pp.71-80
• /
• 2002

Automatic language translation has greatly advanced due to the increased user needs and Information retrieval in WWW. This paper utilizes the integrated knowledge bases of noun and verb networks for automatic translation of ambiguous words in the Korean sentences, through the selectional restriction relation in the sentences. And this paper presents the method to verify validity of Korean noun semantic networks that are used for the construction of the selectional restriction relation by applying the networks to the syntactic and semantic properties Integration of Korean Noun Networks into the SENKOV system will provide the accurate and efficient knowledge bases for the semantic analysis of Korean NLP.

• Biomedical Science Letters
• /
• v.18 no.1
• /
• pp.29-34
• /
• 2012

Streptomycetes are gram-positive filamentous bacteria that are well-known for producing a vast array of bioactive compounds, including more than 70 % of commercially important antibiotics. For the research about Streptomyces sp., the protoplast and electroporation transformation method have been the general techniques for the construction of transformants. However, these techniques have low efficiency and are time-consuming. Another option is intergenic conjugation, which is used for DNA transfer using methylation-deficient E. coli as a DNA donor to avoid the methylated-DNA-dependent restriction systems of actinomycetes. This conjugation method has been widely improved and applied to many other actinomycetes. In this research, an effective transformation procedure for the construction of expression vector by using gateway system was established to avoid limit of restriction enzyme site for cloning of target gene based on transconjugation by Escherichia coli ET12567/pUZ8002 with a pSET152 integration vector.

• Proceedings of the Korean Society of Computer Information Conference
• /
• 2014.07a
• /
• pp.95-96
• /
• 2014

These information systems projects have unique characteristics such as technology sensitiveness, network effectiveness, embeddedness, and externality, these investment projects have been not taken care of in the field of administration and evaluation. Furthermore, it is not easy to evaluate the results of projects under the circumstances where the conditions for evaluation of budget, time, and data leave much to be desired. But the efficient monitoring and effective analysis of information systems are surely needed for beneficient results of investment in information systems. We propose an effect evaluation model on the basis of restriction to evaluate information systems.