Environmental Mutagens and Carcinogens (한국환경성돌연변이발암원학회지)

Korean Environmental Mutagen Society (한국환경성돌연변이발암원학회)
권호 표지

Mitochondrial DNA Analysis in Fusants of Ganoderma lucidum and Lentinus edodes

영지버섯과 표고버섯 원형질 융합체의 미토콘드리아 DNA 검색

  • 최은주 (서울대학교 약학대학) ;
  • 정영자 (식품의약품 안전청 생약규격과) ;
  • 이영재 (제주대학교 농업생명과학 대학) ;
  • 김병각 (서울대학교 약학대학) ;
  • 현진원 (제주대학교 의과대학)
  • Published : 2002.09.01
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Abstract

It has been known that Ganoderma lucidum and Lentinus edodes have anticancer activity and immune enhancing activity. These two mushrooms were grown in liquid culture and harvested. From these mycelia, DNA was isolated and EtBr-CsCl density gradient ultracentrifugation was performed to purify it further. Then mitochondrial DNA was isolated by bisbenzimide-CsCl density ultracentrifugaton. Mitochondrial DNA of Ganoderma lucidum was digested by restriction enzymes, EcoR I, Hind Ⅲ, and Pst I, then electrophoresed. It showed 12, 22, 4 fragments. Mitochondrial DNA of Lentinus edodes was digested by EcoR I. Electric pattern showed 6 fragments. 4 fragments had appeared by Pst 1 digested mitochondrial DNA. Hind ill couldn't digest mitochondrial DNA of Lentinus edodes. Mitochondrial DNA of fusants was isolated to compare to those of parents. The results showed that fusant P₂S₄has new, recombined mitochondrial DNA. But P₂S₄had the same DNA that Ganoderma lucidum had.

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