• 한국섬유공학회:학술대회논문집
• /
• 한국섬유공학회 1987년도 제2차학술발표초록집
• /
• pp.9-10
• /
• 1987

• Journal of Ginseng Research
• /
• 제27권3호
• /
• pp.93-97
• /
• 2003

Alternative or complementary medicine plays an important role in health care system. Ginseng, being one of the most popular oriental herbs, is believed to contain various steroid hormone activity. Ginseng has been demonstrated pharmacological effect in the cardiovascular, endocrine, central nervous, and immune system. Our objective was to study that total saponin might mediate some of their actions by binding to the steroid hormone receptor, as they share many of the actions of steroid hormone in various physiological system. Using total saponin from Panax Ginseng, we have studied the possibility of total saponin being a potential estrogen receptor, androgen receptor, and retinoic acid receptor ligand. Total saponin activated the transcription of both the estrogen and androgen responsive luciferase reporter plasmids at a concentration of 100$\mu\textrm{g}$/ml in COS cells transiently transfected with the corresponding receptor and hormone responsive receptor plasmids. And total saponin caused a concentration-dependent stimulation of estrogen receptor. Total saponin increased the expression of estrogen responsive c-fos proto-oncogene at the protein level in MCF7 cells at 24 h treatment as examined by Western analysis. The c-fos induction was used as a specific marker of estrogen responsiveness. This activation was inhibited by the specific estrogen receptor antagonist, ICI 182,780. However, total saponin failed to activate the retinoic acid receptor in COS cells transiently transfected with the corresponding receptor and retinoic acid responsive reporter plasmids. These results show that total saponin is capable of activating estrogen and androgen receptors.

• Journal of Korean Neurosurgical Society
• /
• 제61권2호
• /
• pp.201-211
• /
• 2018

Objective : The purpose of this study was to analyze the variability of clopidogrel responses according to duration of a clopidogrel drug regimen after stent-assisted coil embolization (SAC), and to determine the correlation between the variability of clopidogrel responses and thromboembolic or hemorrhagic complications. Methods : A total of 47 patients who underwent SAC procedures to treat unruptured intracranial aneurysms were enrolled in the study. Preoperatively, patients received more than seven days of aspirin (100 mg) and clopidogrel (75 mg), daily. P2Y12 reaction unit (PRU) was checked with the VerifyNow test one day before the procedure (pre-PRU) and one month after the procedure (post-PRU). PRU variability was calculated as the difference between the initial response and the follow-up response. Patients were sorted into two groups based on their response to treatment : responsive and hypo-responsive. Results : PRU variability was significantly greater in the hypo-responsive group when compared to the responsive group (p=0.019). Pre-PRU and serum platelets counts were significantly correlated with PRU variation (p=0.005 and p=0.004, respectively). Although thromboembolic complication had no significant correlated factors, hemorrhagic complication was correlated with pre-PRU (p=0.033). Conclusion : In conclusion, variability of clopidogrel responses during clopidogrel medication was correlated to serum platelet counts and the initial clopidogrel response. Thromboembolic and hemorrhagic complications did not show correlation with the variability of clopidogrel response, or the clopidogrel response after one month of medication; however, hemorrhagic complication was associated with initial clopidogrel response. Therefore, it is recommended to test patients for an initial clopidogrel response only, as further tests would be insignificant.

• 대한교통학회지
• /
• 제25권6호
• /
• pp.111-120
• /
• 2007

본 연구에서는 DGPS 장비를 장착한 개별 차량으로부터 얻어진 주행궤적자료를 활용하여 VMS영향권에서의 운전자 반응특성을 분석하였다. 실험 대상 구간은 서울도시고속도로(올림픽대로)상의 직선부 도로의 VMS를 대상으로 설정하였다. ANOVA TEST를 통하여 VMS 접근거리에 대한 운전자 반응특성(가속도, 속도)을 분석하였으며, 접근 거리에 따른 가속도의 확률밀도함수를 도출하였다. 본 연구의 분석결과는 VMS 설치로 인해 운전자가 체험하는 작업부하가 교통류패턴에 미치는 영향을 도출하는데 활용 될 수 있으며 나아가 이러한 운전자의 반응행태분석은 보다 현실적인 미시적 교통류 모의실험을 위한 기초자료로도 유용하게 사용될 것으로 기대된다.

• 미생물학회지
• /
• 제46권1호
• /
• pp.104-108
• /
• 2010

Leucine-responsive regulatory protein (Lrp)는 대장균 (Escherichia coli)에서 발견된 '글로벌 조절자(global regulator)'로서 Lrp-regulon이 leucine에 의하여 상이한 형태의 조절 양상을 나타낸다. 6XHis-tag 시스템으로 제조한 야생형 Lrp (Lrp Wt)와 돌연변이 Lrp (Lrp R145W) 단백질을 정제하여 그들의 생화학적 성질을 조사하였다. 이들은 gel retardation assay를 통하여 ilv 오페론의 프로모터 영역 consensus 염기서열인 21bp의 이중가닥 DNA와 결합하여 복합체를 형성하는 것을 확인 하였다. 형광성 아미노산인 tryptophan을 지닌 Lrp R145W은 단백질의 농도가 증가함에 따라 형광이 커졌으며, 아미노산 leucine에 의하여 형광성의 변화가 관찰되었다. 즉 1 ${\mu}M$의 Lrp R145W 단백질에 leucine을 첨가하여 결합시키면 약 20 ${\mu}M$까지는 형광이 증가하다가 그 이상의 농도에서는 감소하는 양상을 얻었다. 이들 실험 결과는 leucine과 Lrp의 결합 양상 및 구조변이에 관한 심층연구에 있어서 Lrp의 고유 형광성이 요긴하게 쓰일 수 있음을 시사한다.

• 가정과삶의질연구
• /
• 제28권1호
• /
• pp.37-49
• /
• 2010

This study investigated the effects of three factors - children's age, sex and parental feeding styles - on children's self-help skills. The subjects were 213 children and their parents who lived in Kwanak-ku, Seoul. The three-way analysis of variance was employed. The main results were as follows: 1. Children's autonomy was varied significantly according to their sex. Girls do better by themselves than boys do in toileting, washing and drying hands, putting on and buttoning up a coat, putting on clothes, washing and drying their faces. Children's autonomy also varied significantly according to age. A majority of the autonomy subsets increased as the children's ages increased. However, drying their hands with a towel, removing their coats, and drinking water did not vary by age. 2. Children's autonomy also varied significantly according to their parents' feeding style. A responsive feeding style encouraged children to drink water by themselves. 3. The group with the highest score in self-help skills in toileting was 6-year-old girls, and the group with the lowest score was 3-year-old boys. Additionally, the groups with the highest scores in toileting were 4-year-old girls whose parents demonstrated a neglective feeding style, 5-year-old girls whose parents demonstrated an authoritative feeding style, 5-year-old girls whose parents demonstrated a responsive feeding style, 3-year-old boys whose parents demonstrated a responsive feeding style and 6-year-old boys whose parents demonstrated a neglective feeding style. Conversely, the groups with the lowest scores in toileting were 3-year-old girls whose parents demonstrated an authoritarian feeding style, 5-year-old girls whose parents demonstrated a neglective feeding style, and 3-year-old and 6-year-old boys whose parents demonstrated an authoritarian feeding style. 4. The group with the highest score in removing their coats was girls whose parnets demonstrated authoritative, responsive or neglective feeding styles. The group with the lowest score in removing their coats was boys whose parents demonstrated authoritative or neglective feeding styles.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
• /
• pp.429.2-429.2
• /
• 2014

ZnO nanowire is known as synthesizable and good mechanical properties. And, stimuli-responsive polymer is widely used in the application of tunable sensing device. So, we combined these characteristics to make precise tunable sensing devise. In this work, we investigate the dependence of ZnO nanowire alignment and morphology on si substrate using nanosphere template with various conditions via hydrothermal process. Also, pH-temperature dependant tuning ability of nanostructure was studied. The brief experimental scheme is as follow. First, Zno seed layer was coated on a si wafer ($20{\times}20mm$) by spin coater. And then $1.15{\mu}m$ sized close-packed PS nanospheres were formed on a cleaned si substrate by using gas-liquid-solid interfacial self-assembly method. After that, zinc oxide nanowires were synthesized using hydrothermal method. Before the wire growth, to specify the growth site, heat treatment was performed. Finally, NIPAM(N-Isopropylacrylamide) was coated onto as-fabricated nanostructure and irradiated by UV light to form the PNIPAM network. The morphology, structures and optical properties are investigated by FE-SEM(Field Emission Scanning electron Microscopy), XRD(X-ray diffraction), OM(Optical microscopy), and WCA(water contact angle).

• 한국도로학회논문집
• /
• 제14권3호
• /
• pp.163-171
• /
• 2012

수요대응형 교통수단(Demand Responsive Transit)은 변화하는 이동수요에 대응하는 탄력적인 교통수단으로 단순히 노약자와 장애인을 위한 복지교통 서비스의 영역이 아니라, 무선통신과 위치정보서비스(Location Based Service: LBS)의 발달로 인하여 도심형 수단으로 보다 효율적인 교통수단으로 자리매김하고 있다. 그러나 문전서비스(Door-to-Door)를 제공하는 수요대응형 교통수단 시뮬레이션에 적합한 상용툴의 부재로 인하여 알고리즘이나 차량 운행 요소를 면밀하게 분석하기 힘든 어려움이 있었다. 본 연구는 수요대응형 교통수단에 연관된 다양한 차량 운영계획과 알고리즘을 구현, 평가할 수 있는 시뮬레이션 환경을 제안한다. 문전서비스(Door-to-Door) 기반의 차량 운행 모형을 적용하기 위하여 확보되어야 하는 시뮬레이션 입력 데이터를 정의하고 있으며, 수요대응형 교통수단의 대표적인 범주에 속하는 실시간 합승 택시(Shared-Taxi) 서비스를 서울시 교통망과 택시 수요를 이용하여 적용하였다. 합승 택시 운행 계획을 위하여 Nearest Vehicle Dispatch(NVD)와 Insertion Heuristic(IH), 두 종류의 알고리즘을 제안하였으며, 제안된 시뮬레이션을 통하여 성능을 비교하였다. 또한, 합승(Ride-sharing)을 허용하지 않는 일반적인 택시와의 비교를 통하여 시스템 효율 향상과 서비스 품질 변화를 분석하였다.

• The Korean Journal of Pain
• /
• 제29권2호
• /
• pp.103-109
• /
• 2016

Background: The location and the number of lumbar sympathetic ganglia (LSG) vary between individuals. The aim of this study was to determine the appropriate level for a lumbar sympathetic ganglion block (LSGB), corresponding to the level at which the LSG principally aggregate. Methods: Seventy-four consecutive subjects, including 31 women and 31 men, underwent LSGB either on the left (n = 31) or the right side (n = 43). The primary site of needle entry was randomly selected at the L3 or L4 vertebra. A total of less than 1 ml of radio opaque dye with 4% lidocaine was injected, taking caution not to traverse beyond the level of one vertebral body. The procedure was considered responsive when the skin temperature increased by more than $1^{\circ}C$ within 5 minutes. Results: The median responsive level was significantly different between the left (lower third of the L4 body) and right (lower margin of the L3 body) sides (P = 0.021). However, there was no significant difference in the values between men and women. The overall median responsive level was the upper third of the L4 body. The mean responsive level did not correlate with height or BMI. There were no complications on short-term follow-up. Conclusions: Selection of the primary target in the left lower third of the L4 vertebral body and the right lower margin of the L3 vertebral body may reduce the number of needle insertions and the volume of agents used in conventional or neurolytic LSGB and radiofrequency thermocoagulation.

• Molecules and Cells
• /
• 제24권2호
• /
• pp.185-193
• /
• 2007

Symbiotic nitrogen fixation with nitrogen-fixing bacteria in the root nodules is a distinctly beneficial metabolic process in legume plants. Legumes control the nodule number and nodulation zone through a systemic negative regulatory system between shoot and root. Mutation in the soybean NTS gene encoding GmNARK, a CLAVATA1-like serine/threonine receptor-like kinase, causes excessive nodule development called hypernodulation. To examine the effect of nts mutation on the gene expression profile in the leaves, suppression subtractive hybridization was performed with the trifoliate leaves of nts mutant 'SS2-2' and the wild-type (WT) parent 'Sinpaldalkong2', and 75 EST clones that were highly expressed in the leaves of the SS2-2 mutant were identified. Interestingly, the expression of jasmonate (JA)-responsive genes such as vspA, vspB, and Lox2 were upregulated, whereas that of a salicylate-responsive gene PR1a was suppressed in the SS2-2 mutant. In addition, the level of JA was about two-fold higher in the leaves of the SS2-2 mutant than in those of the WT under natural growth conditions. Moreover, the JA-responsive gene expression persists in the leaves of SS2-2 mutant without rhizobia infection in the roots. Taken together, our results suggest that the nts mutation increases JA synthesis in mature leaves and consequently leads to constitutive expression of JA-responsive genes which is irrelevant to hypernodulation in the root.