• Fisheries and Aquatic Sciences
• /
• v.13 no.1
• /
• pp.56-62
• /
• 2010

To determine whether long double-stranded RNA (dsRNA) induces RNA interference and type I interferon (IFN) responses in fish, long dsRNAs encoding enhanced green fluorescent protein (EGFP), GFPuv, and polyinosinic-polycytidylic acid sequences were co-injected with an EGFP expressing plasmid, into rock bream (Oplegnathus fasciatus). We investigated the EGFP mRNA and protein levels, and the transcriptional responses of dsRNA-dependent protein kinase and Mx1 genes. Long dsRNAs were strong inducers of a type I IFN response in rock bream, resulting in nonspecific suppression of exogenous gene expression. Furthermore, sequence-specific knockdown of exogenous gene expression at the mRNA level was detected at an early phase (24 h). These results suggested that long dsRNA may inhibit exogenous gene expression through an early mRNA interference response and a later type I IFN response in fish.

• Wind and Structures
• /
• v.28 no.2
• /
• pp.111-128
• /
• 2019

In this paper an octagon plan shaped building (study building) in presence of three square plan shaped building is subjected to boundary layer wind flow and the interference effects on the study building is investigated using Computational fluid dynamics. The variation of the pressure coefficients on different faces of the octagon building is studied both in isolated and interference conditions. Interference Factors (IF) are calculated for different faces of the study building which can be a powerful tool for designing similar plan shaped buildings in similar conditions. A metamodel of the IF, in terms of the distances among buildings is also established using Response Surface Method (RSM). This set of equations are optimized to get the optimum values of the distances where the IF is unity. An upstream Interference zone for this building setup and wind environment is established from these data. Uncertainty principle is also utilised to determine the optimum positions of the interfering buildings considering the uncertain nature of wind flow for minimum interference effect. The proposed procedure is observed to be computationally efficient in deciding optimum layout at buildings often required in city planning. The results show that the proposed RSM-based optimization approach captures the interference zone accurately with substantially less number of experiments.

• Structural Engineering and Mechanics
• /
• v.54 no.5
• /
• pp.891-908
• /
• 2015

For a systematic study on wind-induced vibration characteristics of large hyperbolic cooling towers with different feature sizes, the pressure measurement tests are finished on the rigid body models of three representative cooling towers with the height of 155 m, 177 m and 215 m respectively. Combining the refined frequency-domain algorithm of wind-induced responses, the wind-induced average response, resonant response, background response, coupling response and wind vibration coefficients of large cooling towers with different feature sizes are obtained. Based on the calculating results, the parametric analysis on wind-induced vibration of cooling towers is carried out, e.g. the feature sizes, damping ratio and the interference effect of surrounding buildings. The discussion shows that the increase of feature sizes makes wind-induced average response and fluctuating response larger correspondingly, and the proportion of resonant response also gradually increased, but it has little effect on the wind vibration coefficient. The increase of damping ratio makes resonant response and the wind vibration coefficient decreases obviously, which brings about no effect on average response and background response. The interference effect of surrounding buildings makes the fluctuating response and wind vibration coefficient increased significantly, furthermore, the increase ranges of resonant response is greater than background response.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.22 no.5
• /
• pp.663-668
• /
• 2012

A TPMS(Tire Pressure Monitoring System) is a wireless communication system designed to monitor the pressure and temperature of pneumatic tires of a vehicle. In order to provide the aid in protecting a driver, this system reports tire pressure information to the driver of the vehicle. Since the wireless communication technique should be employed to transmit the TPMS data from each tire to signal processing unit in the vehicle, it suffers from interference signals from external electrical or electronics equipments. In this paper, we propose the TPMS interference cancellation technique based on GSC(Generalized Sidelobe Canceler), which does not have only the excellent performance like MVDR(Minimum-Variance-Distortionless-Response) but also has the low computational complexity comparing with MVDR. The performance of interference suppression is conformed by computer simulation examples.

• Wind and Structures
• /
• v.28 no.2
• /
• pp.71-87
• /
• 2019

The yaw and interference effects of blades affect aerodynamic performance of large wind turbine system significantly, thus influencing wind-induced response and stability performance of the tower-blade system. In this study, the 5MW wind turbine which was developed by Nanjing University of Aeronautics and Astronautics (NUAA) was chosen as the research object. Large eddy simulation on flow field and aerodynamics of its wind turbine system with different yaw angles($0^{\circ}$, $5^{\circ}$, $10^{\circ}$, $20^{\circ}$, $30^{\circ}$ and $45^{\circ}$) under the most unfavorable blade position was carried out. Results were compared with codes and measurement results at home and abroad, which verified validity of large eddy simulation. On this basis, effects of yaw angle on average wind pressure, fluctuating wind pressure, lift coefficient, resistance coefficient,streaming and wake characteristics on different interference zone of tower of wind turbine were analyzed. Next, the blade-cabin-tower-foundation integrated coupling model of the large wind turbine was constructed based on finite element method. Dynamic characteristics, wind-induced response and stability performance of the wind turbine structural system under different yaw angle were analyzed systematically. Research results demonstrate that with the increase of yaw angle, the maximum negative pressure and extreme negative pressure of the significant interference zone of the tower present a V-shaped variation trend, whereas the layer resistance coefficient increases gradually. By contrast, the maximum negative pressure, extreme negative pressure and layer resistance coefficient of the non-interference zone remain basically same. Effects of streaming and wake weaken gradually. When the yaw angle increases to $45^{\circ}$, aerodynamic force of the tower is close with that when there's no blade yaw and interference. As the height of significant interference zone increases, layer resistance coefficient decreases firstly and then increases under different yaw angles. Maximum means and mean square error (MSE) of radial displacement under different yaw angles all occur at circumferential $0^{\circ}$ and $180^{\circ}$ of the tower. The maximum bending moment at tower bottom is at circumferential $20^{\circ}$. When the yaw angle is $0^{\circ}$, the maximum downwind displacement responses of different blades are higher than 2.7 m. With the increase of yaw angle, MSEs of radial displacement at tower top, downwind displacement of blades, internal force at blade roots all decrease gradually, while the critical wind speed decreases firstly and then increases and finally decreases. The comprehensive analysis shows that the worst aerodynamic performance and wind-induced response of the wind turbine system are achieved when the yaw angle is $0^{\circ}$, whereas the worst stability performance and ultimate bearing capacity are achieved when the yaw angle is $45^{\circ}$.

• Journal of Environmental Science International
• /
• v.16 no.8
• /
• pp.1001-1009
• /
• 2007

Passive sampler is a simple and cost-effective measuring equipment for ambient and indoor air pollution. We studied the performance of a short term (1 hour mean concentration) ozone passive sampler which was coated with a colorant (indigo carmine) to a filter substrate. Acetone and sulfamic acid added ozone passive sampler was investigated to measure short term mean ozone concentration. Ozone response and interference of criteria air pollutant($SO_2,\;NO_2$, CO) on a short term ozone passive sampler was tested through experimental chamber. The results show sulfamic acid added passive ozone sampler have good response in ozone exposure. Interference of $NO_2$ gas is larger than other two criteria gases.

• Journal of fish pathology
• /
• v.23 no.3
• /
• pp.273-280
• /
• 2010

To determine whether rock bream Oplegnathus fasciatus can be protected from rock bream iridovirus (RBIV) infection by intramuscular injection of long double-stranded RNAs (dsRNAs), we compared protective effect of virus-specific dsRNAs corresponding to major capsid protein (MCP), ORF 084, ORF 086 genes, and virus non-specific green fluorescent protein (GFP) gene. Furthermore, to determine whether the non-specific type I interferon (IFN) response was associated with protective effect, we estimated the activation of type I IFN response in fish using expression level of IFN inducible Mx gene as a marker. As a result, mortality of fish injected with dsRNAs and challenged with RBIV was delayed for a few days when comparing with PBS injected control group. However, virus-specific dsRNA injected groups exhibited no significant differences in survival period when compared to the GFP dsRNA injected group. Semi-quantitative analysis indicated that the degree of antiviral response via type I IFN response is supposedly equal among dsRNA injected fish. These results suggest that type I IFN response rather than sequence-specific RNA interference might involve in the lengthened survival period of fish injected with virus-specific dsRNAs.

• International Journal of Aeronautical and Space Sciences
• /
• v.17 no.2
• /
• pp.253-259
• /
• 2016

The typical method for performing an absolute radiometric calibration of a Synthetic Aperture Radar (SAR) System is to analyze its response, without interference, to a target with a known Radar Cross Section (RCS). To minimize interference, an error-free calibration site for a Corner Reflector (CR) is required on a wide and flat plain or on an area without disturbance sources (such as ground objects). However, in reality, due to expense and lack of availability for long periods, it is difficult to identify such a site. An alternative solution is the use of a Triangular Trihedral Corner Reflector (TTCR) site, with a surrounding protection wall consisting of berms and a hollow. It is possible in this scenario, to create the minimum criteria for an effectively error-free site involving a conventional object-tip reflection applied to all beams. Sidelobe interference by the berm is considered to be the major disturbance factor. Total interference, including an object-tip reflection and a sidelobe interference, is analyzed experimentally with SAR images. The results provide a new guideline for the minimum criteria of TTCR site design that require, at least, the removal of all ground objects within the fifth sidelobe.

• Wind and Structures
• /
• v.24 no.5
• /
• pp.405-430
• /
• 2017

The aerodynamic characteristics of vehicles are critical to assess vehicle safety and passenger comfort for vehicles running on long span bridges in a windy environment. However, in previous wind-vehicle-bridge (WVB) system analysis, the aerodynamic interference between the vehicle and the bridge was seldom considered, which will result in changing aerodynamic coefficients. In this study, the aerodynamic coefficients of a high-sided truck on the ground (ground case) and a typical bridge deck (bridge deck case) are determined in a wind tunnel. The effects of existent structures including the bridge deck and bridge accessories on the high-sided vehicle's aerodynamic characteristics are investigated. A three-dimensional analytical framework of a fully coupled WVB system is then established based on the finite element method. By inputting the aerodynamic coefficients of both cases into the WVB system separately, the vehicle safety and passenger comfort are assessed, and the critical accidental wind speed for the truck on the bridge in a windy environment is derived. The differences in the bridge response between the windward case and the leeward case are also compared. The results show that the bridge deck and the accessories play a positive role in ensuring vehicle safety and improving passenger comfort, and the influence of aerodynamic interference on the response of the bridge is weak.

• ETRI Journal
• /
• v.35 no.5
• /
• pp.775-785
• /
• 2013

Because of their exclusive features, millimeter wave directive mesh networks can be considered for small cell backhaul support in urban environments. For this purpose, a network of closely spaced stations has been considered with very directive line-of-sight links operating in the 60-GHz band. An attempt is made to evaluate channel response and interference behavior in such a network, taking into account the effect of building blockage. A simple grid of building blocks is considered as the propagation environment, and wave propagation is simulated using 2.5-dimensional (2.5D) ray tracing (2D with ground effect) to calculate the received signal at different nodes in the network. The results are compared with free space predictions and used to evaluate interference at all nodes in the channel and describe certain characteristics of links, such as the delay profile and the correlation length.