• 한국동물생명공학회지
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• 제38권4호
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• pp.209-214
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• 2023

Background: Single nucleotide polymorphisms (SNPs) are widely used genetic markers with applications in human disease diagnostics, animal breeding, and evolutionary studies, but existing genotyping methods can be labor-intensive and costly. The aim of this study is to develop a simple and rapid method for identification of a single nucleotide change. Methods: A modified Polymerase Chain Reaction Amplification of Multiple Specific Alleles (PAMSA) and high resolution melt (HRM) analysis was performed to discriminate a bovine polymorphism in the NCAPG gene (rs109570900, 1326T > G). Results: The inclusion of tails in the primers enabled allele discrimination based on PCR product lengths, detected through agarose gel electrophoresis, successfully determining various genotypes, albeit with some time and labor intensity due to the use of relatively costly high-resolution agarose gels. Additionally, high-resolution melt (HRM) analysis with tailed primers effectively distinguished the GG genotype from the TT genotype in bovine muscle cell lines, offering a reliable way to distinguish SNP polymorphisms without the need for time-consuming AS-PCR. Conclusions: Our experiments demonstrated the importance of incorporating unique mismatched bases in the allele-specific primers to prevent cross-amplification by fragmented primers. This efficient and cost-effective method, as presented here, enables genotyping laboratories to analyze SNPs using standard real-time PCR.

• 정보과학회 논문지
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• 제44권5호
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• pp.496-502
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• 2017

포인터 네트워크(Pointer Networks)는 Recurrent Neural Network (RNN)를 기반으로 어텐션 메커니즘(Attention mechanism)을 이용하여 입력 시퀀스에 대응되는 위치들의 리스트를 출력하는 딥 러닝 모델이다. 대명사 상호참조해결은 문서 내에 등장하는 대명사와 이에 대응되는 선행사를 찾아 하나의 엔티티로 정의하는 자연어처리 문제이다. 본 논문에서는 포인터 네트워크를 이용하여 대명사와 선행사의 참조관계를 밝히는 대명사 상호참조해결 방법과 포인터 네트워크의 입력 연결순서(chaining order) 여섯가지를 제안한다. 실험 결과, 본 논문에서 제안한 방법 중 연결순서 coref2 가 MUC F1 81.40%로 가장 좋은 성능을 보였다. 이는 기존 한국어 대명사 상호참조해결의 규칙 기반(50.40%)보다 31.00%p, 통계 기반(62.12%) 보다 19.28%p 우수한 성능임을 나타낸다.

• 대한방사선기술학회지:방사선기술과학
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• 제22권1호
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• pp.5-11
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• 1999

This paper reviews recent major activities on instrumentation and methodology of PET. The performance of the PET instrumentation can be expressed by four physical characteristics, 1) spatial resolution, 2) coincidence resolving time, 3) energy resolution, and 4) detection efficiency. The physical and technical aspects of PET systems are briefly discussed along with these characteristics. Toward high resolution PET the recent trend has been to design multiple rings of densely packed detector arrays with scintillators. In order to satisfy the sampling requirement in reconstruction, continuous detector units has been developed. Iterative image reconstruction algorithms have received considerable attention for improvement of both the sampling requirement and image quality toward the stationary PET. Better resolving time improves the maximum true coincidence rate, which is also increased with more detectors placed in coincidence with each other. It suggests that volume PET is promising for enhancement of detection efficiency. The scattered coincidence event rate may be reduced by using detectors with better energy resolution. The use of interplane septa, however, takes over improvement of energy resolution in 2D PET. Energy resolution becomes an important factor for image quality under the condition of septa removal such as volume PET. Toward full utilization of emitting photons, 3D reconstruction incorporating oblique rays has been studied, and volume reconstruction algorithms have been developed. Practical volume PET systems impose heavy burden not only to detector sets and coincidence circuits, but also to computers in the memory requirements and the data processing. In conclusion, there have been many ingenious methods in development of PET instrumentation, which are based on unique capability of PET. They will be expected to overcome technical limitations, and to approach the fundamental limits.

• 대기
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• 제18권2호
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• pp.121-136
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• 2008

We investigate effects of vertical resolution on a parameterization of convective gravity waves (SGWDC) developed in Song and Chun (2005) through offline and online tests of the SGWDC parameterization. For offline tests, numerical simulations of the SGWDC parameterization with different number of vertical levels (L66, L117, L168, L219 and L270) from the surface to 120 km are performed for two different saturation methods. It is found that the wave momentum forcing is overestimated or underestimated in the SGWDC parameterization with different vertical resolutions, depending on the saturation methods. The increase of the vertical resolution modifies the magnitude and distribution of the wave momentum forcing in the parameterization, and this is mainly due to modification of wave saturation levels in the wave saturation processes. However the wave momentum forcing converges in the parameterizations with vertical resolutions higher than L168. For online test, the SGWDC parameterizations with vertical resolutions of L66 and L164 are implemented into a climate model with vertical resolution of L66, separately. In the L164 experiment, the wave momentum forcing decreases in the mid-latitude winter mesosphere in July and zonal mean flows are more realistically reproduced in the tropical regions compared with those in the L66 experiment. These results demonstrate that the wave momentum forcing calculated in the parameterization is sensitive to the vertical resolution, and the implementation of the SGWDC parameterization into high resolution models is required for realistic representation of the gravity wave momentum forcing in large-scale numerical models.

• Investigative Magnetic Resonance Imaging
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• 제23권1호
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• pp.38-45
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• 2019

Purpose: To demonstrate the high-resolution numerical simulation of the respiration-induced dynamic $B_0$ shift in the head using generalized susceptibility voxel convolution (gSVC). Materials and Methods: Previous dynamic $B_0$ simulation research has been limited to low-resolution numerical models due to the large computational demands of conventional Fourier-based $B_0$ calculation methods. Here, we show that a recently-proposed gSVC method can simulate dynamic $B_0$ maps from a realistic breathing human body model with high spatiotemporal resolution in a time-efficient manner. For a human body model, we used the Extended Cardiac And Torso (XCAT) phantom originally developed for computed tomography. The spatial resolution (voxel size) was kept isotropic and varied from 1 to 10 mm. We calculated $B_0$ maps in the brain of the model at 10 equally spaced points in a respiration cycle and analyzed the spatial gradients of each of them. The results were compared with experimental measurements in the literature. Results: The simulation predicted a maximum temporal variation of the $B_0$ shift in the brain of about 7 Hz at 7T. The magnitudes of the respiration-induced $B_0$ gradient in the x (right/left), y (anterior/posterior), and z (head/feet) directions determined by volumetric linear fitting, were < 0.01 Hz/cm, 0.18 Hz/cm, and 0.26 Hz/cm, respectively. These compared favorably with previous reports. We found that simulation voxel sizes greater than 5 mm can produce unreliable results. Conclusion: We have presented an efficient simulation framework for respiration-induced $B_0$ variation in the head. The method can be used to predict $B_0$ shifts with high spatiotemporal resolution under different breathing conditions and aid in the design of dynamic $B_0$ compensation strategies.

• 한국전자파학회논문지
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• 제30권4호
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• pp.275-281
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• 2019

합성 개구면 레이다(synthetic aperture radar: SAR) 위성은 주 야와 날씨에 관계없이 객체의 전자기적 산란분포를 2차원 영상으로 제공할 수 있기 때문에, 광학 위성에 비해 객체 분석에 효과적으로 이용될 수 있다. SAR 위성의 지구 관측주기를 고려한다면, 한 번에 넓은 범위를 관측하는 것이 객체분석에 유리하다. 하지만, 관측범위가 넓어질수록 위성 SAR 영상의 해상도가 저하되는 문제점이 있다. 이는 기존 레이다 신호처리에 이용되었던 해상도 향상 기법을 이용하여 극복될 수 있지만, 아직 해상도 향상 기법을 위성 SAR 영상에 적용하여 그 성능을 분석한 연구는 미미한 실정이다. 따라서 본 논문에서는 위성 SAR 영상에 대한 기존 해상도 기법의 적용 가능성을 탐색하는 연구를 수행한다. 구체적으로, 한국항공우주연구원에서 운용 중인 다목적실용위성 5호(Korea multi-purpose satellite-5: KOMPSAT-5) 영상에 객체 탐지를 수행하고, 외삽(extrapolation), RELAX(relaxation), MUSIC(multiple signal classification) 기법을 적용하여 해상도를 향상시킨 후, 그 성능을 분석한다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제15권11호
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• pp.4065-4083
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• 2021

Super-resolution can improve the clarity of low-resolution (LR) images, which can increase the accuracy of high-level compute vision tasks. Portable devices have low computing power and storage performance. Large-scale neural network super-resolution methods are not suitable for portable devices. In order to save the computational cost and the number of parameters, Lightweight image processing method can improve the processing speed of portable devices. Therefore, we propose the Enhanced Information Multiple Distillation Network (EIMDN) to adapt lower delay and cost. The EIMDN takes feedback mechanism as the framework and obtains low level features through high level features. Further, we replace the feature extraction convolution operation in Information Multiple Distillation Block (IMDB), with Ghost module, and propose the Enhanced Information Multiple Distillation Block (EIMDB) to reduce the amount of calculation and the number of parameters. Finally, coordinate attention (CA) is used at the end of IMDB and EIMDB to enhance the important information extraction from Spaces and channels. Experimental results show that our proposed can achieve convergence faster with fewer parameters and computation, compared with other lightweight super-resolution methods. Under the condition of higher peak signal-to-noise ratio (PSNR) and higher structural similarity (SSIM), the performance of network reconstruction image texture and target contour is significantly improved.

• Journal of Positioning, Navigation, and Timing
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• 제10권4호
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• pp.253-262
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• 2021

Global Navigation Satellite Systems (GNSS) based precise positioning using Real Time Kinematic (RTK) technique has been proposed as an enabler of the formation operation of moving vehicles. In RTK methods, the integer ambiguity of GNSS carrier phase measurements must be resolved. Although there have been many proposed algorithms for the integer ambiguity resolution, the widelane combination of carrier phase measurements and LAMBDA methods have gained the most popularity in literatures when dual frequency GNSS measurements were used. In this paper, we evaluated five alternative methods to determine relative positions of moving base and rover receivers; the round-off scheme of widelane carrier phase, instant least-squares and Kalman filter-based LAMBDA with widelane carrier phase, instant least-squares and Kalman filter-based LAMBDA with dual frequency measurements. The paper presented the performance of each method using flight test data, which showed their strength and weakness in the aspects of time-to-first-fix, ambiguity resolution success ratio, and relative position errors. Based on that, we provided practical recommendations of RTK operations for moving vehicles.

• Genomics & Informatics
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• 제16권4호
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• pp.17.1-17.6
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• 2018

Tumor heterogeneity, the cellular mosaic of multiple lineages arising from the process of clonal evolution, has continued to thwart multi-omics analyses using traditional bulk sequencing methods. The application of single-cell sequencing, in concert with existing genomics methods, has enabled high-resolution interrogation of the genome, transcriptome, epigenome, and proteome. Applied to cancers, these single-cell multi-omics methods bypass previous limitations on data resolution and have enabled a more nuanced understanding of the evolutionary dynamics of tumor progression, immune evasion, metastasis, and treatment resistance. This review details the growing number of novel single-cell multi-omics methods applied to tumors and further discusses recent discoveries emerging from these approaches, especially in regard to immunotherapy.

• 대기
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• 제26권4호
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• pp.697-709
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• 2016

This study estimates and evaluates the extreme value of 30 m-resolution daily maximum and minimum temperatures over South Korea, using inverse distance weighting (IDW), parameter-elevation regression on independent slopes model (PRISM) and generalized extreme value (GEV) method. The three experiments are designed and performed to find the optimal estimation strategy to obtain extreme value. First experiment (EXP1) applies GEV firstly to automated surface observing system (ASOS) to estimate extreme value and then applies IDW to produce high-resolution extreme values. Second experiment (EXP2) is same as EXP1, but using PRISM to make the high-resolution extreme value instead of IDW. Third experiment (EXP3) firstly applies PRISM to ASOS to produce the high-resolution temperature field, and then applies GEV method to make high resolution extreme value data. By comparing these 3 experiments with extreme values obtained from observation data, we find that EXP3 shows the best performance to estimate extreme values of maximum and minimum temperatures, followed by EXP1 and EXP2. It is revealed that EXP1 and EXP2 have a limitation to estimate the extreme value at each grid point correctly because the extreme values of these experiments with 30 m-resolution are calculated from only 60 extreme values obtained from ASOS. On the other hand, the extreme value of EXP3 is similar to observation compared to others, since EXP3 produces 30m-resolution daily temperature through PRISM, and then applies GEV to that result at each grid point. This result indicates that the quality of statistically produced high-resolution extreme values which are estimated from observation data is different depending on the combination and procedure order of statistical methods.