Genomics & Informatics

Korea Genome Organization (한국유전체학회)
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Single-Cell Sequencing in Cancer: Recent Applications to Immunogenomics and Multi-omics Tools

  • Sierant, Michael C. (Department of Genetics, Yale University School of Medicine) ;
  • Choi, Jungmin (Department of Genetics, Yale University School of Medicine)
  • Received : 2018.12.13
  • Accepted : 2018.12.21
  • Published : 2018.12.31
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Abstract

Tumor heterogeneity, the cellular mosaic of multiple lineages arising from the process of clonal evolution, has continued to thwart multi-omics analyses using traditional bulk sequencing methods. The application of single-cell sequencing, in concert with existing genomics methods, has enabled high-resolution interrogation of the genome, transcriptome, epigenome, and proteome. Applied to cancers, these single-cell multi-omics methods bypass previous limitations on data resolution and have enabled a more nuanced understanding of the evolutionary dynamics of tumor progression, immune evasion, metastasis, and treatment resistance. This review details the growing number of novel single-cell multi-omics methods applied to tumors and further discusses recent discoveries emerging from these approaches, especially in regard to immunotherapy.

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References

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