• Journal of Microbiology and Biotechnology
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• 제26권5호
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• pp.938-945
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• 2016

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious threats to rice production. In this study, screening of rice for resistance to BLB was carried out at two different times and locations; that is, in a greenhouse during winter and in an open field during summer. The pathogenicity of Xoo race K1 was tested on 32 Korean rice cultivars. Inoculation was conducted at the maximum tillering stage, and the lesion length was measured after 14 days of inoculation. Five cultivars, Hanareum, Namcheon, Samgdeok, Samgang, and Yangjo, were found to be resistant in both the greenhouse and open-field screenings. Expression of the plant defense-related genes JAmyb, OsNPR1, OsPR1a, OsWRKY45, and OsPR10b was observed in resistant and susceptible cultivars by qRT-PCR. Among the five genes tested, only OsPR10b showed coherent expression with the phenotypes. Screening of resistance to Xoo in rice was more accurate when conducted in open fields in the summer cultivation period than in greenhouses in winter. The expression of plant defense-related genes after bacterial inoculation could give another perspective in elucidating defense mechanisms by using both resistant and susceptible individuals.

• 한국태양에너지학회 논문집
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• 제30권4호
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• pp.71-78
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• 2010

Investigation of the effective soil thermal conductivity(k) is the first step in designing the ground loop heat exchanger(borehole) of a geothermal heat pump system. The line source method is required by New and Renewable Energy Center of Korea Energy Management Corporation in analyzing data obtained from thermal response tests. Another important factor in designing the ground loop heat exchanger is the borehole thermal resistance($R_b$). There are two methods to evaluate $R_b$ : one is to use a line source method, and the other is to use a shape factor of the borehole. In this study, we demonstrated that the line source method produces better results than the shape factor method in evaluating $R_b$. This is because the borehole thermal resistance evaluated with the line source method characteristically reduces the temperature differences between an actual and a theoretical thermal behaviors of the borehole. Evaluation of $R_b$ requires soil volumetric heat capacity. However, the effect of the soil volumetric heat capacity on the borehole thermal resistance is very small. Therefore, it is possible to use a generally accepted average value of soil volumetric heat capacity($=2MJ/m^3{\cdot}K$) in the analysis. In this work, it is also shown that an acceptable range of the initial ignoring time should be in the range of 8~16hrs. Thus, a mean value of 12 hrs is recommended.

• 디지털융복합연구
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• 제12권2호
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• pp.183-191
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• 2014

SNS는 웹을 기반으로 사용자들이 프로필을 창출하고 다른 사람과 커뮤니케이션 할 수 있도록 지원해주는 서비스이다. 지금까지의 대다수 연구들은 SNS의 이용자들이나 그 영향에 관련된 연구 위주로 진행되어 왔다. 그러나 SNS 비이용자도 많으며, SNS이용자라고 해도 모두 SNS를 즐기고 지속적으로 활용하는 것은 아니다. 이와 같은 현상은 SNS사용에 대해 사용자들이 느끼는 혁신에 대한 저항이 존재하기 때문에 발생한다. 본 논문에서는 선행 연구를 바탕으로 시간부족에 대한 지각, SNS에 대한 인지도, 자기효능감, 적합성, SNS에 대한 정보품질, 주관적 규범 및 프라이버시 우려를 지각된 위험과 지각된 유용성의 영향요인으로 선정하였다. 이들 요인이 지각된 위험과 지각된 유용성에 미치는 영향과, 지각된 위험과 지각된 유용성이 다시 사용자 저항에 미치는 영향을 알아보기 위해 중국내 학생과 인터넷 사용자들을 대상으로 실증 분석하였다. 본 논문을 통해 SNS를 사용하지 않는 이유와 SNS 사용에 대한 저항요인을 파악할 수 있다.

• 한국식품조리과학회지
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• 제30권4호
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• pp.369-377
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• 2014

Obesity increases oxidative stress, which could contribute to the development of insulin resistance and hyperglycemia. The purpose of this study was to investigate the hypoglycemic and antioxidant effect of sanchae-namul (SN) in mice with diet-induced obesity. Five-week-old male C57BL/6J mice were fed a basal or high-fat and high-sucrose (HFHS) diet with or without 3% freeze-dried SN powder composed of chamnamul, daraesoon, miyeokchwi, bangpung namul, and samnamul for 12 weeks after a 1-week adaptation. After sacrifice, serum glucose and insulin were measured and the homeostasis model assessment for insulin resistance (HOMA-IR) was determined as well. Hepatic lipid peroxidation, glutathione (GSH), and activities of the antioxidant enzymes were determined. SN given at 3% of the total diet did not significantly influence body weight and food intake in mice fed the HFHS diet. Serum glucose and insulin levels, as well as HOMA-IR values, were significantly lower in the SN group than those in the HFHS group. Thiobarbituric acid reactive substances (TBARS) levels in the liver were decreased significantly in the SN group compared with those in the HFHS group. SN significantly increased the GSH levels and the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the liver compared with those in the HFHS group. Overall, these findings suggest that SN may be useful in alleviating insulin resistance and hyperglycemia in mice fed HFHS diet; further, the improvement of insulin resistance could partly occur by reducing the oxidative stress.

• 마이크로전자및패키징학회지
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• 제12권4호통권37호
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• pp.331-338
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• 2005

The rapid advances in high power light sources and arrays as encountered in incandescent lamps have induced dramatic increases in die heat flux and power consumption at all levels of high power LED packaging. The lifetime of such devices and device arrays is determined by their temperature and thermal transients controlled by the powering and cooling, because they are usually operated under rough environmental conditions. The reliability of packaged electronics strongly depends on the die attach quality, because any void or a small delamination may cause instant temperature increase in the die, leading sooner or later to failure in the operation. Die attach materials have a key role in the thermal management of high power LED packages by providing the low thermal resistance between the heat generating LED chips and the heat dissipating heat slug. In this paper, thermal transient characteristics of die attach in high power LED package have been studied based on the thermal transient analysis using the evaluation of the structure function of the heat flow path. With high power LED packages fabricated by die attach materials such as Ag paste, solder paste and Au/Sn eutectic bonding, we have demonstrated characteristics such as cross-section analysis, shear test and visual inspection after shear test of die attach and how to detect die attach failures and to measure thermal resistance values of die attach in high power LED package. From the structure function oi the thermal transient characteristics, we could know the result that die attach quality of Au/Sn eutectic bonding presented the thermal resistance of about 3.5K/W. It was much better than those of Ag paste and solder paste presented the thermal resistance of about 11.5${\~}$14.2K/W and 4.4${\~}$4.6K/W, respectively.

• 한국조경학회지
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• 제31권4호
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• pp.74-81
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• 2003

The need for insect and mite resistant turfgrass cultivars arose because of problems associated with pesticide use. Representative cultivars and genotypes of 18 warm-season turfgrass [Zoysia japonica Steud., Z. japonica${\times}$Z. metrella hybrids, Z. japonica${\times}$Z. tenuifotia hybrids, Z. matrella (L.) Merr., Cynodon dactylon (L.) Pers., C. dactylon${\times}$C. transvallensis hybrids, Paspalum notatum Flugge., P. vaginatum Swartz., Stenotaphrum secundatum (Walt.) Kuntze, Eremochloa ophiuroides (Munro.) and Buchloe dactyloides (Nutt.) Engelm.] and 20 cool-season turfgrasses [Poa pratensis L., Festuca arundinacea Schreb., F. rubra L., F. rubra var. commutata Gaud., F. ovina var. duriuscula L. Koch. Agrostis tenuis Sibth., A. palustris Huds., and Latium perenne L.] were evaluated for host resistance to feeding by the Spodoptera depravata (Butler) in the laboratory. Two experiments were set up in the laboratory using 8.5cm diameter${\times}$4.0cm deep plastic petri dishes as larvae feeding chambers. In experiment 1, one neonate larvae were place on the grass in each dish and the dishes were arranged with 5 replicates each within an environmental chamber maintained at $25^{\circ}C$ and 15h light: 9h dark Larval survival and larval weights at 7d and 14d, pupal weights, and days to pupation were compared among turfgrasses. In Experiment 2, 4cm sections of all grasses were oriented equidistant from each other in a pattern resembling the spokes of a wheel. Five one neonate larvae were introduced to the center of each dish. Dishes were immediately placed in an environmental chamber held at $25^{\circ}C$, 15h light: 9h dark Larvae were allowed to feed for 24h. Damage was rated from 0(no damage) to 9(completely consumed) were made for eachturfgrass. Resistance as antibiosis (high mortality, slowed growth, and least preference) was identified in Z. japonica${\times}$Z. tenuifolia hybirids ‘Emerald’, Z. japonica${\times}$Z. metrella hybirds ‘Miyako’ and Eremochloa ophiuroides (Munro.). Cool-season turfgrasses tested were susceptible to feeding by Spodoptera depravata (Butler).

• Journal of Dairy Science and Biotechnology
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• 제28권2호
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• pp.7-11
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• 2010

In recent times, antimicrobial resistance has been a concern because of its relation to national health and food safety. In this study, we reviewed the management of antibiotics and antimicrobial-resistant microorganisms in domestic and foreign countries and analyzed microorganisms and antimicrobial resistance in dairy farms and raw milk. The isolates from dairy farms were Micrococcus spp., Staphylococcus chromogens, Escherichia coli, Bacillus licheniformis, Escherichia coli O157:H7, Pseudomonas plecoglossicida, Enterobacter spp., and Kluyvera intermedia. Rummeliibacillus stabekisii, Paenibacillus badius, Pantoea agglomerans, B. oleronius, B. fusiformis, and B. badius were isolated from feed E. coli and Kurthia gibsonii, from feces and S. pasteuri, S. aureus, S. chromogenes, and Salmonella spp., from raw milk. Pathogens isolated from dairy farms and raw milk were tested for susceptibility to 20 types of antibiotics. E. coli (EAEC) and E. coli O157:H7 (EHEC) isolated from dairy farms, E. coli (EAEC) isolated from feces, and Salmonella spp. isolated from raw milk showed resistance to multiple antibiotics. These results show that antimicrobial-resistant microorganisms should be more effectively managed to improve the safety of dairy farms.

• 대한물리의학회지
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• 제10권3호
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• pp.101-108
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• 2015

PURPOSE: This study researched the effects of the changes of elbow joint angle and of arm position in PNF pattern on muscle activation of the contralateral shoulder muscles while performing PNF pattern exercise. METHODS: The research subjects were 16 male physical therapists who had no neuromuscular or neurological disorders. To measure the muscle activation of the contralateral shoulder muscles, EMG electrodes were attached to the muscle valley of the middle and posterior areas of the deltoid and triceps muscles of the arm. Muscle activation while performing the ulnar thrust PNF pattern exercise was measured with the elbow joint positioned at angles of $30^{\circ}$, $45^{\circ}$, and $60^{\circ}$. Resistance points were at the initial, middle, and end ranges of PNF pattern exercise. RESULTS: Muscle activation of the middle and posterior portions of the deltoid muscle increased significantly according to the changes of elbow joint angle. In each resistance point the middle range was significantly higher than at other points. A significant difference on muscle activation was demonstrated throughout each range depending on the type of muscle. Muscle activation of the middle and posterior portions of the deltoid muscle was higher than muscle activation of the triceps. CONCLUSION: The results of this study demonstrate that the PNF pattern exercising method used in this study is a selective exercising method focusing on the deltoid muscle over the triceps muscle. In order to increase the muscle strength to the maximum level, it is necessary to provide the maximum level of resistance in the middle range of the elbow joint.

• 디지털융복합연구
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• 제14권4호
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• pp.371-378
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• 2016

본 연구에서는 한국의 닭에서 분리된 E. coli 균주들로부터 퀴놀론계 항생제 내성을 나타내는 균주를 분리 동정하고 그 내성 기전과 유병률에 관하여 조사하였다. 또한 multilocus sequence typing (MLST)을 이용하여 E. coli 균주들의 분자생물학적 성상을 분석하였다. 항생제 감수성 테스트에서 63.5% (54/85) 의 E. coli 균주들에서 퀴놀론계 항생제 내성률을 보였다. 또한 퀴놀론계 항생제 내성을 보이는 54개 모두에서 gyrA 유전자의 sense mutations과 parC 유전자의 $57^{th}$, $80^{th}$, or $84^{th}$residues에서 점돌연변이를 관찰할 수 있었다. MLST를 통한 분석에서 E. coli ST는 parE 유전자의 염기치환과 깊은 상관관계를 보이는 것으로 관찰되었다. 이 결과들을 바탕으로 우리가 먹는 가축 및 가금류에 대한 무분별한 항생제 사용은 항생제 내성균의 증가와 유전변이를 초래함을 알 수 있었다. 따라서 식용 동물에 대한 지속적인 감시와 모니터링을 통하여 항생제 내성균의 확산방지를 통제하는 것이 필요할 것으로 사료된다.

• 한국해양공학회지
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• 제29권6호
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• pp.418-426
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• 2015

Recently, shipping operators have been making efforts to reduce the fuel cost in various ways, such as trim optimization and bulb re-design. Furthermore, IMO restricts the hydro-dioxide emissions to the environment based on the EEDI (Energy Efficiency Design Index), EEOI (Energy Efficiency Operational Indicator), and SEEMP (Ship Energy Efficiency Management Plan). In particular, ship speed is one of the most important factors for calculating the EEDI, which is based on methods suggested by ITTC (International Towing Tank Conference) or ISO (International Standardization Organization). Many shipbuilding companies in Korea have carried out speed trials around the Korea Straits. However, the conditions for these speed trials have not been exactly the same as those for model tests. Therefore, a ship’s speed is corrected by measured environmental data such as the seawater temperature, density, wind, waves, swell, drift, and rudder angle to match the conditions of the model tests. In this study, fundamental research was performed to evaluate the ship resistance performance due to changes in the water temperature and salinity, comparing the ISO method and numerical simulation. A numerical simulation of a KCS (KRISO Container ship) with a free-surface was performed using the commercial software Star-CCM+ under three conditions that were assumed based on the water temperature and salinity data in the Korea Straits. In the simulation results, the resistance increased under low water temperature & high salinity conditions, and it decreased under high water temperature & low salinity conditions. In addition, the ISO method showed the same result as the simulation.