• Title/Summary/Keyword: resistance management

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The Probiotic and Adherence Properties of Lactobacillus reuteri Pg4 Expressing the Rumen Microbial β-Glucanase

  • Yu, B.;Liu, J.R.;Hsiao, F.S.;Lee, T.T.;Chiou, P.W.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.9
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    • pp.1324-1329
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    • 2008
  • This study was conducted to evaluate the potential of the transformed Lactobacillus reuteri Pg4 (T-Pg4) harboring the ${\beta}$-glucanase gene as a poultry probiotic. The probiotic properties of the T-Pg4 strain were evaluated in vitro by their adherence capability and acid and bile salt tolerance, and were evaluated in vivo by their survival and adhesion in the gastrointestinal tract (GIT) of specific-pathogen-free (SPF) chickens. The results showed that the T-Pg4 strain exhibited resistance to acidic conditions and contact with bile salt, and adhered efficiently to the crop and intestinal epithelial cells of chickens in vitro. The T-Pg4 strain also could survive and colonize the gastrointestinal epithelium of the experimental SPF chickens in vivo. In addition, radial enzyme diffusion was used to demonstrate that the Lactobacillus spp. randomly isolated from the GIT of the SPF chickens fed T-Pg4 possessed ${\beta}$-glucanase secretion capability. These findings have demonstrated that the transformed L. reuteri Pg4 survives transit through the stomach and intestine, and may secrete ${\beta}$-glucanase in the chicken GIT. Therefore, it is suggested that this organism could be used as a multifunctional poultry probiotic.

Milk Quality and Antimicrobial Resistance against Mastitis Pathogens after Changing from a Conventional to an Experimentally Organic Dairy Farm

  • Suriyasathaporn, Witaya
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.5
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    • pp.659-664
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    • 2010
  • The present study was to investigate the effect of the transition from conventional to organic dairy farming on the antimicrobial resistant pattern of pathogens in milk. A farm with tie-stall management, with an average herd size of 20 milking cows, was selected based on the owner' willingness to accept, for at least 6 months, the highly restricted protocol developed in this study. Comparisons of bacterial isolates and antimicrobial susceptibilities before changing to an organic farm system (BEFORE) and for 6 months after (AFTER) operating the experimental organic farm system were performed by Fisher's Exact Chi-square tests. Significant levels were defined at p<0.05. During the AFTER period, average frequency of antibiotic treatment was decreased from more than 3 cases/month to less than 1 case/month during which the antibiotic use was authorized only by the veterinarian. In total, 92 and 70 quarter milk samples from 24 and 18 cows during BEFORE and AFTER, respectively, were included in the study. Overall, isolates ranged from a non-resistant level for cephazolin to a very high resistant level to streptomycin (64.71% to 95.45%). Percentages of antimicrobial resistant isolates during BEFORE were significantly higher than during AFTER for ampicillin (43.48% and 5.88%, respectively) and streptomycin (95.45% and 64.71%, respectively). In conclusion, percentages of antimicrobial resistant isolates were decreased after 6 months of operating as an organic farm system.

A Electrical and Optical studies of WO3/Ag/WO3 Transparent Electrode by RF Magnetron Sputtering (RF 마그네트론 스퍼터링을 이용한 WO3/Ag/WO3 투명전극의 전기·광학적 특성 연구)

  • Kang, Dong-Soo;Lee, Boong-Joo;Kwon, Hong-Kyu;Shin, Paik-Kyun
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.63 no.11
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    • pp.1533-1537
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    • 2014
  • $WO_3/Ag/WO_3$ multilayer was researched by using RF magnetron sputtering with transparent electrode. Process gas flow ratio with $Ar/O_2$ were selected the optimum conditions at 70sccm/2sccm and $WO_3$ thin film at its conditions was appeared at transmittance about 80% in the visible light region to the average. $WO_3/Ag/WO_3$ multilayer thin films were fabricated from the same process condition which was the same gas flow ratio of Ar and $O_2$ $WO_3/Ag/WO_3$ thin films were appeared transmittance about 93% and sheet resistance about $6.41{\Omega}/{\square}$. From the SEM images, each thin films were appeared when $WO_3$ is 40nm and $O_2$ is 10nm.

Duplication-Aware Garbage Collection for Flash Memory-Based Virtual Memory Systems (플래시 메모리 기반의 가상 메모리 시스템을 위한 중복성을 고려한 GC 기법)

  • Ji, Seung-Gu;Shin, Dong-Kun
    • Journal of KIISE:Computer Systems and Theory
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    • v.37 no.3
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    • pp.161-171
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    • 2010
  • As embedded systems adopt monolithic kernels, NAND flash memory is used for swap space of virtual memory systems. While flash memory has the advantages of low-power consumption, shock-resistance and non-volatility, it requires garbage collections due to its erase-before-write characteristic. The efficiency of garbage collection scheme largely affects the performance of flash memory. This paper proposes a novel garbage collection technique which exploits data redundancy between the main memory and flash memory in flash memory-based virtual memory systems. The proposed scheme takes the locality of data into consideration to minimize the garbage collection overhead. Experimental results demonstrate that the proposed garbage collection scheme improves performance by 37% on average compared to previous schemes.

A Study on Measurement of Mechanical Properties of Polyther Polyol Base Polyurethane Dispersion by 1,4-butanediol Chain Extension (Polyether Polyol을 이용한 수분산 폴리우레탄의 1,4-butanediol 사슬연장에 의한 물성변화)

  • Lee, Joo-Youb
    • Journal of the Korean Applied Science and Technology
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    • v.31 no.4
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    • pp.711-718
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    • 2014
  • In this paper, the mechanical property change was investigated by adding 1,4-butanediol as chain extender of polyurethane dispersion for leather coating. Reagents for the synthesis were polypropylene glycol(PPG), isoporon diisocyanate(IPDI), dimethylolpropionic acid(DMPA) and 1,4-BD. All samples showed good solvent resistance and flexibility by different content of the 1,4-BD. As results, samples which contains a lot of 1,4-BD showed good properties whth strength of $1.80kg_f/mm^2$ and abrasion test measurement of 49.54 mg.loss. According to elongation test, the sample which contains low amount of 1,4-BD was good property as 364 %.

Antimicrobial susceptibility and detection of enterotoxin by multiplex PCR of Staphylococcus aureus isolated from dogs and cats in Seoul (서울지역의 개와 고양이에서 분리한 Staphylococcus aureus의 항생제 감수성 및 multiplex PCR을 이용한 장독소의 검출)

  • Kim, Neung-Hee;Chae, Hee-Sun;Son, Hong-Rak;Kim, Chang-Ki;Kim, Sun-Heung;Lee, Jung-Hark;Kim, Chul-Hun
    • Korean Journal of Veterinary Service
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    • v.33 no.3
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    • pp.263-269
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    • 2010
  • The antimicrobial susceptibility of 73 strains of Staphylococcus aureus isolated from dogs and cats was determined against 16 antimicrobial agents. All isolates were susceptible to cephalothin (CF), vancomycin (VA), rifampin (RA), quinupristin-dalfopristin (SYN) and linezolid (LZD), whereas more than 86.3% of the isolates were resistant to penicillin (P). In the case of enterotoxin production, 8.2% of 73 strains produced enterotoxin A and 1 strain produced enterotoxin C. Appropriate protocols for antimicrobial agents and strategies to reduce antimicrobial resistance will need to be investigated in the future.

Effectiveness of Different Classes of Fungicides on Botrytis cinerea Causing Gray Mold on Fruit and Vegetables

  • Kim, Joon-Oh;Shin, Jong-Hwan;Gumilang, Adiyantara;Chung, Keun;Choi, Ki Young;Kim, Kyoung Su
    • The Plant Pathology Journal
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    • v.32 no.6
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    • pp.570-574
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    • 2016
  • Botrytis cinerea is a necrotrophic pathogen causing a major problem in the export and post-harvest of strawberries. Inappropriate use of fungicides leads to resistance among fungal pathogens. Therefore, it is necessary to evaluate the sensitivity of B. cinerea to various classes of fungicide and to determine the effectiveness of different concentrations of commonly used fungicides. We thus evaluated the effectiveness of six classes of fungicide in inhibiting the growth and development of this pathogen, namely, fludioxonil, iprodione, pyrimethanil, tebuconazole, fenpyrazamine, and boscalid. Fludioxonil was the most effective ($EC_{50}$ < $0.1{\mu}g/ml$), and pyrimethanil was the least effective ($EC_{50}=50{\mu}g/ml$), at inhibiting the mycelial growth of B. cinerea. Fenpyrazamine and pyrimethanil showed relatively low effectiveness in inhibiting the germination and conidial production of B. cinerea. Our results are useful for the management of B. cinerea and as a basis for monitoring the sensitivity of B. cinerea strains to fungicides.

Performance Evaluation of Long-Life Asphalt Concrete Overlays Based on Field Survey Monitoring in National Highways (일반국도 현장조사 모니터링을 통한 장수명 아스팔트 덧씌우기 포장의 공용성 분석)

  • Baek, Jongeun;Lim, Jae Kyu;Kwon, Soo Ahn;Kwon, Byung Yoon
    • International Journal of Highway Engineering
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    • v.17 no.3
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    • pp.69-76
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    • 2015
  • PURPOSES : Performance evaluation of four types of asphalt concrete overlays for deteriorated national highways. METHODS : Pavement distress surveys for crack rate and rut depth have been conducted annually using an automated pavement survey vehicle since 2007. Linear and non-linear performance prediction models of the asphalt concrete overlays were developed for 43 sections. The service life of the asphalt overlays was defined as the number of years after which a crack rate of 30% or rut depth of 15mm is observed. RESULTS : The service life of the asphalt overlays was estimated as 17.4 years on an average. In 90.7% of the sections, the service life of the overlays was 15 years or more which is 1.5 times the life of conventional asphalt concrete overlays used in national highways. The performance of the overlays was dependent on the type of asphalt mixture, traffic volume levels, and environmental conditions. CONCLUSIONS : The usage of stone mastic asphalt (SMA) and polymer-modified asphalt (PMA) for the overlays provided good resistance to cracking and rutting development. It is recommended that appropriate asphalt concrete overlays must be applied depending on the type of existing pavement distress.

Surgical Management of Ventricular Septal Defect in Infancy (영아기의 심실중격결손에 대한 이라완전교정술과 단계교정술의 비교)

  • 김병호
    • Journal of Chest Surgery
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    • v.27 no.1
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    • pp.24-30
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    • 1994
  • Eighteen infants with a large ventricular septal defect[VSD] underwent primary surgical repair from January 1986 to December 1992. Operation was done because of failure to thrive, medically intractable heart failure, recurrent pneumonia, increased pulmonary vascular resistance[PVR]. Four patients[22.2%] died in the early postoperative period. Relief of heart failure and normalization of growth and weight gain was evident in all survivor. There was no late postoperative death. The results of primary surgical repair of VSD in infancy are compared with those of palliative pulmonary artery banding[PAB] and of VSD closure after PAB. Twenty-seven patients with isolated VSD or with VSD associated with atrial septal defect, patent ductus arteriosus, or coarctation of the aorta underwent initial palliative PAB. There were 3 early postoperative deaths[11.1%]. Severe elevation of PVR persisted in two patients. Closure of VSD and pulmonary artery debanding was done in twenty patients, with 2 early postoperative deaths[10.0%]. Placement of the PAB too close to the pulmonary annulus necessitated trasannular patching in one patient, but any problem caused by migration of the band was not developed. It is concluded that primary surgical repair of VSD in infancy is reasonable and that PAB is indicated only for those patients less than 6 months old with a complicated defect or in an emergency situation.

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Antecedents of Fashion Brand Immunity to Negative Information and Impact on Brand Commitment (부정적 정보에 대한 패션브랜드 면역에의 영향요인과 브랜드 몰입과의 관계 연구)

  • Kim, Han-Na;Lee, Eun-Jung
    • Journal of the Korean Society of Clothing and Textiles
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    • v.35 no.7
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    • pp.856-866
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    • 2011
  • This study examines how brands can be managed to influence the degree of their resistance to negative information and the extent to which this immunity to negative information predicts the purchase intention of consumers. In particular, the study investigated the difference between brand commitment and brand immunity on negative information in terms of the concept and the power of influence. A total of 410 usable questionnaires were obtained through an internet survey. A structural equation model using a correlation matrix with maximum likelihood was estimated using AMOS 18.0 to examine the relationship among a brand's social responsibility, quality orientation, brand innovativeness, easy purchase intention, and difficult purchase intention. The results showed that brand's social responsibility had significant effects on brand immunity to negative information while quality orientation and brand innovativeness had significant effects on brand commitment. In addition, brand immunity had significant effects on brand commitment and then brand commitment had a significant effect on easy and difficult purchase intentions. The key implications for research and management are discussed.