• Journal of Bio-Environment Control
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• v.25 no.2
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• pp.111-117
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• 2016

The objective of this study was to analyze the microclimate and the growth of tomato and lettuce in the greenhouses covered with spectrum conversion films using different phosphor particles sizes. Two spectrum conversion films using phosphor particles larger than $10{\mu}m$ (Micro-film) and smaller than 500 nm (Nano-film) in radius, and poly-ethylene (PE) film were used in double-layered greenhouses as outer coverings. PE films were used as inner coverings in all the greenhouses. Thickness of the films for inner and outer coverings was 0.06 mm. Tensile strength, elongation, and tearing resistance of the Micro- and Nano-films were not different from those of the PE film. Transmittances at a wavelength of 300-1100 nm were a little higher at the Micro-film and lower at the Nano-film than that of the PE film, respectively. Air temperatures at the Micro- and Nano-films were over $2^{\circ}C$ higher than at the PE film, but no significant difference was observed between the two light conversion films. The soil temperature at the Nano-film was $1.5^{\circ}C$ and $3^{\circ}C$ higher than at the Micro- and PE films, respectively. The yields of tomato at the Micro- and Nano-films were 12% and 14% higher than at the PE film, but no significant difference was observed between the two spectrum conversion films. The total soluble solid showed no significant differences among all the films. The yields of lettuces at the Micro- and Nano-films were 27% and 59% higher than at the PE film. Hunter's red (a) value of the lettuce leaf was the highest at the Nano-film. In this experiment, tomatoes requiring high irradiation were better at the Nano film, while lettuce requiring low irradiation better at the Micro film.

• Journal of fish pathology
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• v.10 no.1
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• pp.1-14
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• 1997

Major object of this study was to investigate the causative organism of th e diseased cultured fingerling of sea bass, L japonicus. The experimental results are summarized as follows ; Staphylococcus epidermidis, isolated from the liver, kidney, spleen and brain, was considered to be the causative organism. External symptoms of this disease were congestion and hemorrhages in eyes. Anatomical symptoms were hemorrhage of brain, congestion of liver, and slight swelling of kidney and spleen. Growth of the isolates was good on BHIA, HIA and Staphylococcus No. 110. The growth occurred at a range(optimum) of $10\sim45^{\circ}C(35\sim40^{\circ}C)$, 0~9% (1~3%) of NaCl concentration and pH 4~10(8). DNase and coagulase production of all isolated strains were nagative, but was positive in hemolysis. Urease was positive reaction, and novobiocin resistance was nagative. Acid was produced anaerobically from glucose and maltose. Acid was produced aerobically from glucose, galactose, sucrose, maltose and dextrine. But gas was not produced from any carbohydrates. When the isolated strain was injected intramuscularly on fingerling of sea bass, L japonicus, it had virulence at $1.7{\times}10^{10}$ viable cells/$m\ell$ for all fish examined but no virulence at $1.7{\times}10^4$ viable cells/$m\ell$. Bacitracin, Erythromycin and Nofloxacin were observed as bacteriostatic agents to the strain, but Colistin, Gentamicin and Nalidixic acid were not. There were remarkable congestion of the brain, regressive necrosis of the liver, and showed necrosis of the epithelial cells of renal tubules in kidney tissues.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.4
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• pp.265-272
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• 2002

Forage breeding laboratory of National Livestock Research Institute, R.D.A. has made interspecific hybrids of Lolium multiflorum $\times$ L. pratensis and intergeneric hybrids of Lolium $\times$ Festuca since 1984, and has collected ecotypes of Italian ryegrass since 1991. Growth characteristics of these hybrids and ecotypes were researched, and then these clone lines were named. Among these clone lines, the several clones that have polen fertility, high cold-tolerance, and similar heading time were used for making synthetics, Naehan 6, 7, 8, 9, with polycrossing method in 1997. Field experiments were carried out to compare the mophological and agronomical characteristics and forage productivity and quality of the synthetics with those of Italian ryegrass varieties, Barmultra and Hwasan 101. in Suwon and Yonchun from 1999 to 2000. Heading time of the synthetics were 22th to 24th May that belong to late-mature types to be similar to that of Barmultra and Hwasan 101 in Suwon. The synthetics were 101 to 106 c3n in plant length, medium or thick in thickness of stem, dark peen in leaf color, broad and long in flag leaf, strong in lodging resistance, and excellent in regrowth. Winter survivals of the synthetics were no different from that of Barmultra or Hwasan 101 in Suwon, but better than that of Barmultra or Hwasan 101 in Yonchun where was -10 to -12$^{\circ}C$ of minimum average air temperature in January or February. Dry matter(DM) yields of the synthetics were similar to DM 8,238kg per ha of Barmultra in Suwon, but in Yonchun, were more 7 to 13% than DM 7,291kg per ha of Barmultra. Forage qualities, IVDMD, ADF, NDF and TDN of the synthetics were lower than those of Hwasan 101, but higher than those of Barmultra.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.1
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• pp.1-8
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• 2017

We investigated the effect of silicate coating of rice seeds on bakanae disease incidence and the quality of seedlings raised in seedling boxes and transplanted into pots. The silicate-coated rice seed (SCS) was prepared as follows. Naturally infested rice seeds not previously subjected to any fungicidal treatment were dressed with a mixture of 25% silicic acid at pH 11 and 300-mesh zeolite powder at a ratio of 50 g dry seed - 9 mL silicic acid - 25 g zeolite powder. The following nursery conditions were provided : Early sowing, dense seeding in a glass house with mulching overnight and no artificial heating, which were the ideal conditions for determining the effect on the seed. The nursery plants were evaluated for Gibberella. fujikuroi infection or to determine the recovery to normal growth of infected nursery plants in the Wagner pot. Seedlings emerged 2-3 days earlier for the SCS than they did for the non-SCS control, while damping-off and bakanae disease incidence were remarkably reduced. Specifically, bakanae disease incidence in the SCS was limited to only 7.8% for 80 days after sowing, as compared to 91.6% of the non-SCS control. For the 45-days-old SCS nursery seedlings, the fresh weight was increased by 11% and was two times heavier, with only mild damage compared to that observed for non-SCS. Even after transplanting, SCS treatment contributed to a lower incidence of further infections and possibly to recovery of the seedlings to normal growth as compared to that observed in symptomatic plants in the pot. The active pathogenic macro-conidia and micro-conidia were considerably lower in the soil, root, and seedling sheath base of the SCS. In particular, the underdeveloped macro-conidia with straight oblong shape without intact septum were isolated in the SCS ; this phenotype is likely to be at a comparative etiological disadvantage when compared to that of typical active macro-conidia, which are slightly sickle-shaped with 3-7 intact septa. A active intact conidia with high inoculum potential were rarely observed in the tissue of the seedlings treated only in the SCS. We propose that promising result was likely achieved via inhibition of the development of intact pathogenic conidia, in concert with the aerobic, acidic conditions induced by the physiochemical characteristics associated with the air porosity of zeolite, alkalinity of silicate and the seed husk as a carbon source. In addition, the resistance of the healthy plants to pathogenic conidia was also important factor.

• Korean Journal of Medicinal Crop Science
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• v.6 no.1
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• pp.11-15
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• 1998

This experiment was conducted to investigate the soil temperature. the characteristics of seedling growth and drought resistance of Job's tears under different seeding depth. The changes of soil temperature were similar to the patterns of atmosphere temperature. and the minimum and maximum temperature of the day were at 08: 00 and 16: 00 hours, respectively, and this was 1 hour late compared to the atmospheric temperature. In mean temperature under different soil depth, 3cm and 4cm soil depth were the same as $27.3^{\circ}C$ which was higher than that at 1cm and 2cm soil depth. Percentage of emergence was 81.2% at 4cm soil depth in Yulmoo 1 ho and 88.0% at 3cm soil depth in Yunchon 9 ho, respectively. Days to emergence after seeding was shortest at 1cm seeding depth as about 9 days and was 2 to 3 days later at other soil depth of 2, 3, 4cm compared to 1cm soil depth plot. Plant height and dry matter weight was highest at 3cm soil depth in Yulmoo 1 ho and at 4cm soil depth in Yunchon 9 ho. In the drought conditions, percent of survived plant was highest at 4cm soil depth plot of both varieties as 51.3% and 60.0%, respectively. In the drought conditions, soil depth was positively correlated with % of emergence, days to emergence after seeding, plant height, and dry matter weight per plant.

• Journal of Bio-Environment Control
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• v.17 no.2
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• pp.162-169
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• 2008

To determine suitable composition of nutrient solution according to soil textures in fertigation culture of cucumber using three strengths (S) of Yamasaki cucumber recipe, chemical changes of soil, growth characteristics and yield of cucumber were investigated. Electric conductivity of drainage solution was risen in all treatments, pH of loam soil was generally optimum level and that of sandy soil was high level. Photosynthetic rate in loam soil supplied the 1/2 S and transpiration rate in sandy soil supplied the 1.0 S were most low. Diffusive resistance in sandy soil supplied the 1.0 S was high. Chlorophyll contents was higher concentrations by the kinds of soil. Amount of drainage solution in sandy loam soil supplied the 1/2 S and loam soil supplied the 1.0 S were most much and little, respectively. Water absorption rate was the opposition to amount of drainage solution. Nutrient contents in soil except calcium were most high in the 1.0 S by the kinds of soil. Nutrient contents in leaves, nitrogen in sandy soil supplied 1.0 S, phosphorus in loam soil supplied 1/2 S, potassium in two soil supplied 1.0 S, calcium in loam supplied 1/2 S and sandy loam soil supplied 1.0 S, magnesium in loam soil supplied the 1/2 S was high. The growth and yield of fruit were more in loam than in sandy loam soil. Therefore, the suitable compositions of macro-nutrients for fertigation culture of cucumber were determined in loam and sandy loam soils as follows: In loam soil, they were $NO_3$-N 12.3, $NH_4$-N 1.0, P 3.0, K 5.9, Ca 5.7, Mg $3.5\;me{\cdot}L^{-1}$, whereas in sandy loam soil $NO_3$-N 11.7, $NH_4$-N 1.0, P 3.0, K 5.9, Ca 4.9, Mg $3.2\;me\;L^{-1}$.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.61-68
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• 2007

To develop rice (Oryza sativa L.) cultivars to be planted on salt-affected sites, cell lines with enhanced proline content and resistance to growth inhibition by Azetidine-2-carboxylic acid (AZCA), a proline analogue, were screened out among calli irradiated with gamma ray of 50, 70, 90, and 120 Gy. The calli had been derived from embryo culture of the cultivar Donganbyeo. Selected AZCA resistant lines that had high proline accumulation were used as sources for selection of NaCl resistant lines. To determine an optimum concentration for selection of NaCl resistant lines, Donganbyeo seeds were initially cultured on the media containing various NaCl concentrations (0 to 2.5%) for 40 days, and 1.5% NaCl concentration was determined as the optimum concentration. One hundred sixteen salt-tolerant (ST) lines were selected from bulked 20,000 seeds of the AZCA resistant $M_{3}$ seeds in the medium containing 1.5% NaCl. The putative 33 lines ($M_{4}$ generation) considered with salt-tolerance were further analyzed for salt tolerance, amino acid and ion contents, and expression patterns of the salt tolerance-related genes. Out of the 33 lines, 7 lines were confirmed to have superior salt tolerance. Based on growth comparison of the entries, the selected mutant lines exhibited greater shoot length with average 1.5 times, root length with 1.3 times, root numbers with 1.1 times, and fresh weight with 1.5 times than control. Proline contents were increased maximum 20%, 100% and 20% in the leaf, seed and callus, respectively, of the selected lines. Compared to control, amino acid contents of the mutants were 24 to 29%, 49 to 143%, 32 to 60% higher in the leaf, seed and callus, respectively. The ratio of $Na^{+}/K^{+}$ for most of the ST-lines were lower than that of control, ranging from 1.0 to 3.8 for the leaf and 11.5 to 28.5 for the root, while the control had 3.5 and 32.9 in the leaf and root, respectively. The transcription patterns for the P5CS and NHXI genes observed by RT-PCR analysis indicated that these genes were actively expressed under salt stress. The selected mutants will be useful for the development of rice cultivar resistant to salt stress.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.240-240
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• 2017

Quinoa (Chenopodium quinoa Willd.) is one of the ancient crops cultivated in the Andes region at an altitude of 3,500-4000m in Chile and Bolivia from 5000 BC. It contains a large amount of protein, minerals and vitamins in comparison with other crops. The cultivation area has been increasing worldwide because of its excellent resistance to various abiotic stress such as salinity, drought and low temperature. ${\gamma}$-Ray radiation of high dose is often used as a tool to induce mutations in plant breeding, but it has a deleterious effect on organisms. However, the radiation may have a positive stimulatory effect of 'hormesis' in the low dose range. This experiment was carried out to investigate the optimum dose range for creating the quinoa genetic resources and to investigate the hormesis effect at low dose on the quinoa. This experiment was performed for 120 days from November, 2016 to February, 2017 in the greenhouse of Gyeongsang National University. ${\gamma}$-Ray radiation was irradiated to seeds at 0 Gy, 50 Gy, 100 Gy, 200 Gy, 300 Gy, 400 Gy, 600 Gy, 800 Gy and 1000 Gy for 8 hours. (50 Gy) using the low level radiation facility ($Co^{60}$) of Cooperative Research Institute of Radiation Research Institute, KAERI. Fifty seeds were placed on each petri dish lined with wet filter paper and germination rate was measured at a time interval of 2 hours for 40 hrs. The length of the root length was measured one week after germination. Each treatment was carried out in 3 replicates. The growth of seedlings were investigated for 10 days after transplanting of 30 day-old seedlings. The plant height, NDVI, SPAD, Fv/Fm, and panicle weight were measured. The germination rate was highest at 50Gy and 0Gy and the rate of seeds treated with 400Gy or higher rate decreased to 25% of the seeds treated with 50Gy. The emergence rate of seedling in pot experiment was higher at the dose of 200 Gy, 300 Gy and 400 Gy than at 0 and 50Gy. However, the rate was lower at strong radiation higher than 600Gy at which $1^{st}$ leaf was not expanded fully and dead due to extreme overgrowth at 44 days after treatment (DAT). The highest value of panicle weight was observed at 50Gy (6.15g) and 100Gy (5.57g). On the other hand, the weight at high irradiated dose of 300Gy and 400Gy was decreased by about 55% compared to low dose (50 Gy). NDVI measurement also showed the highest value at 50 Gy as the growth progressed. SPAD was the highest at 400 Gy and showed positive correlation with irradiation dose except 0 Gy. Fv/Fm was high at 50 Gy up to 30 DAT and no difference between treatments was observed except for 400 Gy from 44 DAT. The plant height was the highest in 50Gy during the growing period and was higher in the order of 50Dy, 100Gy, 0Gy, 200Gy, 300Gy and 400Gy in 88 DAT. In this experiment, the optimal radiation dose for hormesis was 50Gy and 100Gy, and the optimal radiation dose for mutagenesis seems to be 400 Gy.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.05a
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• pp.89-119
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• 2004

This study was conducted to isolate lactobacilli having probiotic characteristics to be used as health adjuncts with fermented milk products. Acid tolerant strains were selected in Lactobacilli MRS broth adjusted to pH 4.0 from 80 healthy persons (infants, children and adults). And bile tolerant strains were examined in Lactobacilli MRS broth in which 1.0% bile salt was added. By estimation above characteristics, the strains No. 27, which was isolated from adult feces, was selected and identified as Lactobacillus salivarius subsp. salivarius based on carbohydrate fermentation and 16S rDNA sequencing. It was used as a probiotic strain in fermented milk products. The pH of fermented milk decreased from pH 6.7 to 5.0 and titratable acidity increased from 0.3% to 1.0% by L. salivarius subsp. salivarius (isolation strain 20, 35, and 37), when incubated for 36 h at $37^{\circ}C$. The number of viable cell counts of fermented milk was maximized at this incubation condition. The SDS-PAGE evidenced no significant change of casein but distinct changes of whey protein were observed by isolated L. salivarius subsp. salivarius for titratable acidity being incubated by $0.9{\sim}1.0%$ at $37^{\circ}C$. All of the strains produced 83.43 to 131.96 mM of lactic acid and 5.39 to 26.85 mM of isobutyric acid in fermented products. The in vitro culture experiment was performed to evaluate ability to reduce cholesterol levels and antimicrobial activity in the growth medium. The selected L. salivarius subsp. salivarius reduced $23{\sim}38%$ of cholesterol content in lactobacilli MRS broth during bacterial growth for 24 hours at $37^{\circ}C$. All of the isolated L. salivarius subsp. salivarius had an excellent antibacterial activity with $15{\sim}25$ mm of inhibition zone to E. coli KCTC1039, S. enteritidis KCCM3313, S. typhimurium M-15, and S. typhimurium KCCM40253 when its pH had not been adjusted. Also, all of the isolated L. salivarius subsp. salivarius had partial inhibition zone to E. coli KCTC1039, E. coli KCTC0115 and S. enteritidis KCCM3313 when it had been adjusted to pH 5.7. The selected strains were determined to have resistances of twelve antibiotic. Strains 27 and 35 among the L. salivarius subsp. salivarius showed the highest resistance to the antibiotics. Purified ${\alpha}$-galactosidase was obtained by DEAE-Sephadex A-50 ion exchange chromatography, Mono-Q ion exchange chromatography and HPLC column chromatography from L. salivarius subsp. salivarius 27. The specific activity of the purified enzyme was 8,994 units/mg protein, representing an 17.09 folds purification of the original cell crude extract. The molecular weight of enzyme was identified about 53,000 dalton by 12% SDS-PAGE. Optimal temperature and pH for activity of this enzyme were $40^{\circ}C$ and 7.0 respectively. The enzyme was found to be stable between 25 and $50^{\circ}C$. ${\alpha}$-galactosidase activity was lost rapidly below pH 5.0 and above pH 9.0. This enzyme was liberated galactose from melibiose, raffinose, and stachyose, and also the hydrolysis rate of substrate was compound by HPLC. These results indicated that some of the L. salivarius subsp. salivarius (strain 27 and 35) are considered as effective probiotic strains with a potential for industrial applications, but the further study is needed to establish their use as probiotics in vivo.

• Korean Journal of Plant Resources
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• v.33 no.2
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• pp.80-85
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• 2020

A new Gomchwi cultivar 'Soomany' was derived from a cross between Gomchwi (Ligularia fischeri (Ledeb.) Turcz.) and Handaeri-gomchwi (Ligularia fischeri var. spiciformis Nakai). The investigation and selection of growth and yield characteristics were conducted from 2007 to 2015 in field and greenhouse of Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration, in Korea. On a newly developed cultivar 'Soomany', color of petiole ear is light green, petiole trichome is existent, trichome and light on the back of the leaves don't exist, and density of leaf vein is degree 4. Plant height, leaf length, leaf width and petiole length are 77.1, 22.3, 21.5 and 57.2 cm, respectively in the growth characteristics of the 2_nd year. Plant size was also higher than that of 'Sammany' generally. Bolting date was on August 15. Flowering date was on September 19, about 9 days later than 'Sammany'. 'Soomany' and 'Sammany' had 149 and 133 leaves per plant, respectively. Total yield of 'Soomany' (1,623 g/plant) made a very good comparison with that of 'Sammany' (1,385 g/plant). 'Soomany' showed harder leaves (25.8 ㎏/㎠) than 'Sammany' (20.8 ㎏/㎠), whereas 'Soomany' had thinner leaves (0.53 mm) than 'Sammany' (0.62 mm). 'Soomany' variety has shown strong resistance to powdery mildew disease compared to 'Sammany'. In May 2019, the right of variety protection of 'Soomany' was registered as a new Gomchwi variety (Register No. 192).