• 한국전자통신학회논문지
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• 제11권8호
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• pp.801-806
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• 2016

본 연구는 대학생들을 대상으로 소셜TV 혁신저항 결정요인과 이용의도의 관계를 살펴보았다. 주요 결과를 제시하면 다음과 같다. 첫째, 소셜TV 이용에 대한 적합성과 시험가능성, 관찰가능성은 혁신저항에 통계적으로 유의한 부적 영향을 미치는 것으로 나타난 반면에 복잡성은 혁신저항에 통계적으로 유의한 정적 영향을 미치는 것으로 나타났다. 둘째, 소셜TV 이용에 대한 적합성, 복잡성, 시험가능성, 관찰가능성 중 관찰가능성만이 이용의도에 통계적으로 유의한 정적 영향을 미치는 것으로 나타났다. 셋째, 소셜TV에 대한 혁신저항은 이용의도에 통계적으로 유의한 부적 영향을 미치는 것으로 나타났다.

• 약학회지
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• 제35권1호
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• pp.22-29
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• 1991

pMB4 is a 2.4-kilobase plasmid of Staphylococcus aureus TR-1 that confers inducible resistance to the macrolide-lincosamide-streptogramin B(MLS) antibiotics. By subcloning studies, it was found that the MLS resistance determinant was located at 1.0Kb fragment between Sau3AI and TaqI sites. DNA sequence of the MLS resistant determinant, named ermC-4 was determined, and found to be highly homologous with that of ermC. Because the leader peptide sequence of ermC-4 was identical with that of ermC, the expression of the resistance gene is thought to be controlled by posttranscriptional attenuation in S. aureus TR-1.

• 한국콘텐츠학회논문지
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• 제13권6호
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• pp.158-166
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• 2013

본 연구는 사람들이 소셜미디어 수용을 왜 거부하는지를 혁신저항모델과 지각된 위험이론을 적용하여 탐색하였다. 모든 새로운 기술이나 혁신은 수용과정에서 채택 또는 저항이라는 과정을 거친다. 그러므로 저항이라는 것도 수용을 위한 하나의 과정으로 볼 수 있다. 이에 따라 본 연구는 소셜미디어를 적용하여 수용자들이 왜 혁신을 거부 또는 저항하는지를 탐색하였다. 이를 위해 소셜미디어(트위터) 비이용자인 대학생 268명의 자료를 분석에 활용하였다. 주요결과로는 다음과 같다. 첫째, 소셜미디어에 대한 상대적 이점은 혁신저항에 통계적으로 유의미한 부적 영향을 미치는 것으로 나타났다. 둘째, 소셜미디어에 대한 인지적 복잡성은 혁신저항에 통계적으로 유의미한 영향을 미치지 못한 것으로 나타났다. 셋째, 소셜미디어에 대한 지각된 위험은 혁신저항에 통계적으로 유의한 정적 영향을 미치는 것으로 나타났다. 결론적으로 소셜미디어가 사회적 대인관계 매체로서 보다 강화되기 위해서는 상대적 이점을 높이고, 명예훼손이나 인식공격과 같은 위험요소를 줄일 수 있도록 해야 할 것이다.

• Journal of Microbiology and Biotechnology
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• 제12권1호
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• pp.31-38
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• 2002

Bacteria have evolved various types of resistance mechanism to toxic heavy metals, such as arsenic and antimony. An arsenical and antimonial resistant bacterium was isolated from a shallow creek draining a coal-mining area near Taebaek City, in Kangwon-Do, Korea. The isolated bacterium was identified and named as Pseudomonas sp. KM20 after biochemical and physiological studies were conducted. A plasmid was identified and its function was studied. Original cells harboring the plasmid were able to grow in the presence of 15 mM sodium arsenite, while the plasmid-cured (plasmidless) strain was sensitive to as little as 0.5 mM sodium arsenate. These results indicated that the plasmid of Pseudomonas sp. KM20 does indeed encode the arsenic resistance determinant. In growth experiments, prior exposure to 0.1 mM arsenate allowed immediate growth when they were challenged with 5 mM arsenate, 5 mM arsenite, or 0.1 mM antimonite. These results suggested that the arsenate, arsenite, and antimonite resistance determinants of Pseudomonas sp. KM20 plasmid were indeed inducible. When induced, plasmid-bearing resistance cells showed a decreased accumulation $of\;73^As$ and showed an enhanced efflux $of\;^73As$. These results suggested that plasmid encoded a transport system that extruded the toxic metalloids, resulting in the lowering of the intracellular concentration of toxic oxyanion. In a Southern blot study, hybridization with an E. coli R773 arsA-specific probe strongly suggested the absence of an arsA cistron in the plasmid-associated arsenical and antimonial resistance determinant of Pseudomonas sp. KM20.

• 약학회지
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• 제32권4호
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• pp.213-221
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• 1988

Inducible MLS resistance gene of Bacillus licheniformis specified by erm K was subcloned in Bacillus subtilis and the DNA sequence corresponding to its control region was determined. The determinant erm K was in Pvu II=Hind III fragment, which was 1.3 kb. The leader region is capable of forming a complex series of inverted complementary repeat sequences (ICRS) centering on at least six axes of symmetry, some of them mutually exclusive, in a way that resulted ultimately in post-transcriptional unmasking of the ribosome loading site for methylase synthesis.

• Archives of Pharmacal Research
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• 제15권1호
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• pp.58-61
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• 1992

Bacillus anthracis 590 having an inducibla resistance determinant to MLS antibiotics was isolated from a soli sample in Korea. The resistance gene (ernJ) was cloned by Southern blotting of chromosomal DNA fragment digested by various restriction enzymes and coloy hybridization method and the cloned plasmid was named as pBA423. The size of inserted DNA fragment of pBS42 vector was about 2.9 kb and the DNA sequence of the subcloned fragment (Hinc II-Hinc II, 1.4kb) WAS determined. The DNA sequence of ernJ was composed of 357 bp for leader region and 861 bp for the structural gene. Because the leader sequence of ernJ was homologous to that of ermK, the expression of ernJ is also thought to be controlled by a transcriptionl attenuation mechanism.

• Journal of Microbiology and Biotechnology
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• 제18권6호
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• pp.1040-1043
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• 2008

Klebsiella oxytoca CCUG 15788 is resistant to $Ni^{2+}$ at a concentration of 10 mM and grows in an inducible manner when exposed to lower concentrations of $Ni^{2+}$. The complete genomic sequence of a 4.2-kb HindIII-digested fragment of this strain was determined from genomic DNA. It was shown to contain four nickel resistance genes (nirA, nirB, nirC, and nirD) encoding transporter and transmembrane proteins for nickel resistance. When the plasmid pKOHI4, encoding nirABCD, was transformed into Escherichia coli JM109, the cells were able to grow in Tris-buffered mineral medium containing 3 mM nickel. TnphoA'-1 insertion mutants in the four nickel genes nirA, nirB, nirC, and nirD showed nickel sensitivity. The nir genes were heterogeneously expressed in E. coli, suggesting functional roles of these genes in nickel resistance.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.282.2-282
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• 1999

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.327.2-327
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• 1998

No Abstract, See Full Text

• 미생물학회지
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• 제25권2호
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• pp.87-93
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• 1987

The properties of the plasmid pKU41 isolated from Pseudomonas putkda KU190 have been investigated, pKU41 was defined as an R plasmid having a transmissible ampicillin and tetracycline resistance determinant, and could be classified as a plasmid belonging to IncP-1 group according to incompatibility grouping.