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Plasmid-Mediated Arsenical and Antimonial Resistance Determinants (ars) of Pseudomonas sp. KM20  

Yoon, Kyung-Pyo
Publication Information
Journal of Microbiology and Biotechnology / v.12, no.1, 2002 , pp. 31-38 More about this Journal
Abstract
Bacteria have evolved various types of resistance mechanism to toxic heavy metals, such as arsenic and antimony. An arsenical and antimonial resistant bacterium was isolated from a shallow creek draining a coal-mining area near Taebaek City, in Kangwon-Do, Korea. The isolated bacterium was identified and named as Pseudomonas sp. KM20 after biochemical and physiological studies were conducted. A plasmid was identified and its function was studied. Original cells harboring the plasmid were able to grow in the presence of 15 mM sodium arsenite, while the plasmid-cured (plasmidless) strain was sensitive to as little as 0.5 mM sodium arsenate. These results indicated that the plasmid of Pseudomonas sp. KM20 does indeed encode the arsenic resistance determinant. In growth experiments, prior exposure to 0.1 mM arsenate allowed immediate growth when they were challenged with 5 mM arsenate, 5 mM arsenite, or 0.1 mM antimonite. These results suggested that the arsenate, arsenite, and antimonite resistance determinants of Pseudomonas sp. KM20 plasmid were indeed inducible. When induced, plasmid-bearing resistance cells showed a decreased accumulation $of\;73^As$ and showed an enhanced efflux $of\;^73As$. These results suggested that plasmid encoded a transport system that extruded the toxic metalloids, resulting in the lowering of the intracellular concentration of toxic oxyanion. In a Southern blot study, hybridization with an E. coli R773 arsA-specific probe strongly suggested the absence of an arsA cistron in the plasmid-associated arsenical and antimonial resistance determinant of Pseudomonas sp. KM20.
Keywords
Pseudomonas sp. KM20; arsenical and antimonial resistance; uptake; efflux;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
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1 Methods for General and Molecular Bacteriology /
[ Gerhardt, P.;R. G. E. Murray;W. A. Wood;N. R. Krieg ] / American Society for Microbiology
2 Effect of ionic copper toxicity on the growth of green alga, Selenastrum capricornutum /
[ Kim, M.-K.;Ralph E. H. Smith ] / J. Microbiol. Biotechnol.   과학기술학회마을
3 The ars operon in the skin element of Bacillus subtilis confers resistance to arsenate and arsenite /
[ Sato, T.;Y. Kobayashi ] / J. Bacteriol.
4 Esolation and characterization of antibiotic and heavy metal-resistant Pseudomonas aeruginosa from different polluted waters in Sohag District Egypt /
[ Soltan, El-Sayed. M. ] / J. Microbiol. Biotechnol.   과학기술학회마을
5 The chromosomal arsR gene of Escherichia coli encodes a trans-acting metalloregulatory protein /
[ Xu, C.;W. Shi;B. P. Rosen ] / J. Biol. Chem.   DOI
6 Bacterial heavy metal resistance: New surprises /
[ Silver, S.;L. T. Phung ] / Annu. Rev. Microbiol.   DOI   ScienceOn
7 Inducible chromate reducing activity in Pseudomonas aeruginosa isolated from a leather tannery effluent /
[ Ganguli, A.;A. K. Tripathi ] / J. Microbiol. Biotechnol.   과학기술학회마을
8 Inducible plasmid-determined resistance to arsenate, arsenite, and antimony (Ⅲ) in Escherichia coli and Staphylococcus aureus /
[ Silver, S.;K. Budd;K. M. Leahy;W. V. Shaw;D. Hammond;R. P. Novick;G. R. Willsky;M. H. Malamy;H. Rosenberg ] / J. Bacteriol.
9 /
[ Hughes, M. N.;R. K. Poole ] / Metals and Microorganism
10 Arsenic uptake and transfer in a simplified estuarine food chain /
[ Lindsay, D. A.;J. G. Cooksey ] / Environ. Toxicol. Chem.   DOI
11 Molecular characterization of an anion pump. The arsA gene product is an arsenite (antimonate)-stimulated ATPase /
[ Rosen, B. P.;U. Weigel;C. Karkaria;P. Gangola ] / J. Biol. Chem.
12 An Escherichia coli chromosomal operon homolog is functional in arsenic detoxification and is conserved in gram-negative bacteria /
[ Diorio, C.;J. Cai;J. Marmor;R. Shinder;M. S. DuBow ] / J. Bacteriol
13 The arsenical resistance operon of IncN plasmid R46 /
[ Bruhn, D. F.;J. Li;S. Silver;F. Roberto;B. P. Rosen ] / FEMS Microbiol. Lett.   DOI
14 Energy-dependent arsenate efflux: The mechanism of plasmid mediated resistance /
[ Silver, S.;D. Keach ] / Proc. Natl. Acad. Aci.   DOI   ScienceOn
15 Regulation of the cadA cadmium resistance determinant of Staphylococcus aureus /
[ Yoon, K. P.;T. K. Misra;S. Silver ] / J. Bacteriol.   DOI
16 Construction materials in estuaries: Reduction in the epibiotic community on chromated copper arsenate (CCA) treated wood /
[ Weis, J. S.;P. Weis ] / Mar. Ecol. Prog. Ser.   DOI
17 Orphan enzyme or patriarch of a new tribe: the arsenic resistance ATPase of bacteria plamids /
[ Silver, S.;G. Ji;S. Broer;S. Dey;D. Don;B. P. Rosen ] / Mol. Microbiol.   DOI   ScienceOn
18 The ars operon of Escherichia coli confers arsenical and antimonial resistance /
[ Carlin, A.;W. Shi;S. Dey;B. P. Rosen ] / J. Bacteriol.   DOI
19 Arsenical-resistant trypanosomes lack an unusual adenosine transporter /
[ Carter, N. S.;A. H. Fairlamb ] / Nature   DOI   ScienceOn
20 Catabolic plasmid-mediated heavy metal resistance in herbicide diuron-degrading Pseudomonas species /
[ El-Deeb, Bahig A. ] / J. Microbiol. Biotechnol.   과학기술학회마을
21 /
[ Krieg, N. R.;J. G. Holt ] / Bergey's Manual of Systematic Bacteriology
22 Expression and regulation of the antimonite, arsenite, and arsenate resistance operon of Staphylococcus xylosus plasmid pSX267 /
[ Rosenstein, R.;A. Peschel;B. Wieland;F. Gotz ] / J. Bacteriol.   DOI
23 Molecular characterization of an anion pump. The ArsB protein is the membrane anchor for the ArsA protein /
[ Tisa, L. S.;B. P. Rosen ] / J. Biol. Chem.
24 Membrane topology of the ArsB protein, the membrane subunit of an anion-translocating ATPase /
[ Wu, J.;L. S. Tisa;B. P. Rosen ] / J. Biol. Chem.
25 Genetic basis of the biodegradation of salicylated in Pseudomonas /
[ Chakrabarty, A. M. ] / J. Bacteriol.
26 Bacterial resistance mechanism for heavy metals of environmental concern /
[ Ji, G.;S. Silver ] / J. Ind. Microbiol.   DOI   ScienceOn
27 The arsD gene encodes a second trans-acting regulatory protein of the plasmid-encoded arsenical resistance operon /
[ Wu, J.;B. P. Rosen ] / Mol. Microbiol.   DOI   ScienceOn
28 ATP-Dependent cadmium transport by the cadA cadmium resistance determinant in everted membrane vesicles of Bacillus subtilis /
[ Tsai, K. J.;K. P. Yoon;A. R. Lynn ] / J. Bacteriol.   DOI
29 /
[ Sambrook, J.;E. F. Fritsch;T. Maniatis ] / Molecular Cloning: A Laboratory Manual(2nd ed.)
30 Nucleotide sequence of the structural genes for an anion pump: The plasmid-encoded arsenical resistant operons /
[ Chen, C. -M.;T. Misra;S. Silver;B. P. Rosen ] / J. Biol. Chem.
31 Isolation and characterization of Pseudomonas sp. KM10, a cadmium and mercury-resistant and phenol-degrading bacterium /
[ Yoon, K. P. ] / J. Microbiol. Biotechnol.   과학기술학회마을
32 Energetics of plasmid-mediated arsenate resistance in Escherichia coli /
[ Mobley, H. L. T.;B. P. Rosen ] / Proc. Natl. Acad. Sci.   DOI   ScienceOn
33 A rapid chemical procedure for isolation and purification of chromosomal DNA from Gram-negative bacilli /
[ Beji, A. D.;D. Izard;F. Gavini;H. Leclere;M. Leseine-Delstanche;J. Krembel ] / Anal. Biochem.   DOI   ScienceOn
34 Reduction of arsenate to arsenite by the ArsC protein of the arsenic resistance operon of Staphylococcus aureus plasmid pI258 /
[ Ji, G.;S. Silver ] / Proc. Natl. Acad. Aci.   DOI   ScienceOn
35 /
[ Poole, R. K.;G. M. Gadd ] / Metals: Microbes Interaction