• Title/Summary/Keyword: resistance determinant

Search Result 72, Processing Time 0.025 seconds

Relationship between the determinant factors of social TV resistance to innovation and intention to use (소셜TV 혁신저항 결정요인과 이용의도의 관계)

  • Park, Jin-Woo
    • The Journal of the Korea institute of electronic communication sciences
    • /
    • v.11 no.8
    • /
    • pp.801-806
    • /
    • 2016
  • The purpose of this study was to examine the relationship between the determinant factors of social TV resistance to innovation and intention to use focused on college students. The results were as follows. First, complexity, trialability and observability among the determinant factors of social TV usage influenced positively on resistance to innovation of social TV, and complexity influenced negatively on resistance to innovation of social TV. Second, only observability among the determinant factors of social TV usage influenced positively on intention to use. Third, resistance to innovation of social TV usage influenced negatively on intention to use.

Cloning of Inducible MLS Antibiotics Resistance Genes and their Expression Control Mechanism - ermC-4, a macrolide-lincosamide-streptogramin B resistance determinant on pMB4 from Staphylococcus aureus TR-1 (MLS계 항생물질 유도내성 유전자의 크로닝과 유전자의 발현조절 기전 - Staphylococus aureus TR-1균주의 프라스미드 pMB4에 존재하는 MLS 내성 유전자 ermC-4)

  • 김수환;최응칠;김병각;심미자
    • YAKHAK HOEJI
    • /
    • v.35 no.1
    • /
    • pp.22-29
    • /
    • 1991
  • pMB4 is a 2.4-kilobase plasmid of Staphylococcus aureus TR-1 that confers inducible resistance to the macrolide-lincosamide-streptogramin B(MLS) antibiotics. By subcloning studies, it was found that the MLS resistance determinant was located at 1.0Kb fragment between Sau3AI and TaqI sites. DNA sequence of the MLS resistant determinant, named ermC-4 was determined, and found to be highly homologous with that of ermC. Because the leader peptide sequence of ermC-4 was identical with that of ermC, the expression of the resistance gene is thought to be controlled by posttranscriptional attenuation in S. aureus TR-1.

  • PDF

Determinant Factors of Innovation Resistance of Social Media (소셜미디어 혁신저항 결정요인에 관한 연구)

  • Jeong, Hwa-Seob
    • The Journal of the Korea Contents Association
    • /
    • v.13 no.6
    • /
    • pp.158-166
    • /
    • 2013
  • This study gave attention to the people who has resistance of the social media that tens to be the use as innovations. Therefore, this study examined the determinant factors of psychological resistance of the social media such as Twitter focused on students as none-users of social media. Total of 268 none-users participated in this study. The results were as follows. First, relative advantage influenced negatively on innovation resistance. Second, perceived complexity influenced significantly not on innovation resistance. Third, perceived risk influenced positively on innovation resistance. Therefore, social media related to social relationship should improve relative advantage, the other way, decrease perceived risk such as defamation and personal attack.

Plasmid-Mediated Arsenical and Antimonial Resistance Determinants (ars) of Pseudomonas sp. KM20

  • Yoon, Kyung-Pyo
    • Journal of Microbiology and Biotechnology
    • /
    • v.12 no.1
    • /
    • pp.31-38
    • /
    • 2002
  • Bacteria have evolved various types of resistance mechanism to toxic heavy metals, such as arsenic and antimony. An arsenical and antimonial resistant bacterium was isolated from a shallow creek draining a coal-mining area near Taebaek City, in Kangwon-Do, Korea. The isolated bacterium was identified and named as Pseudomonas sp. KM20 after biochemical and physiological studies were conducted. A plasmid was identified and its function was studied. Original cells harboring the plasmid were able to grow in the presence of 15 mM sodium arsenite, while the plasmid-cured (plasmidless) strain was sensitive to as little as 0.5 mM sodium arsenate. These results indicated that the plasmid of Pseudomonas sp. KM20 does indeed encode the arsenic resistance determinant. In growth experiments, prior exposure to 0.1 mM arsenate allowed immediate growth when they were challenged with 5 mM arsenate, 5 mM arsenite, or 0.1 mM antimonite. These results suggested that the arsenate, arsenite, and antimonite resistance determinants of Pseudomonas sp. KM20 plasmid were indeed inducible. When induced, plasmid-bearing resistance cells showed a decreased accumulation $of\;73^As$ and showed an enhanced efflux $of\;^73As$. These results suggested that plasmid encoded a transport system that extruded the toxic metalloids, resulting in the lowering of the intracellular concentration of toxic oxyanion. In a Southern blot study, hybridization with an E. coli R773 arsA-specific probe strongly suggested the absence of an arsA cistron in the plasmid-associated arsenical and antimonial resistance determinant of Pseudomonas sp. KM20.

MLS Inducible Resistance Mechanism in Bacillus licheniformis EMR-1 -Cloning of erm K, a MLS Resistance Determinant- (Bacillus licheniformis EMR-1에서의 MLS 유도내성 기전 -erm K의 크로닝-)

  • Choi, Eung-Chil;Kwak, Jin-Hwan;Weisblum, Bernard
    • YAKHAK HOEJI
    • /
    • v.32 no.4
    • /
    • pp.213-221
    • /
    • 1988
  • Inducible MLS resistance gene of Bacillus licheniformis specified by erm K was subcloned in Bacillus subtilis and the DNA sequence corresponding to its control region was determined. The determinant erm K was in Pvu II=Hind III fragment, which was 1.3 kb. The leader region is capable of forming a complex series of inverted complementary repeat sequences (ICRS) centering on at least six axes of symmetry, some of them mutually exclusive, in a way that resulted ultimately in post-transcriptional unmasking of the ribosome loading site for methylase synthesis.

  • PDF

A Macrolide-Lincosamide-Streptogramin B Resistance Determinant Gene (ermJ) Cloned from B, anthracis 590

  • Kim, Hee-Sun;Choi, Eung-Chil;Kim, Byong-Kak;Park, Young-In
    • Archives of Pharmacal Research
    • /
    • v.15 no.1
    • /
    • pp.58-61
    • /
    • 1992
  • Bacillus anthracis 590 having an inducibla resistance determinant to MLS antibiotics was isolated from a soli sample in Korea. The resistance gene (ernJ) was cloned by Southern blotting of chromosomal DNA fragment digested by various restriction enzymes and coloy hybridization method and the cloned plasmid was named as pBA423. The size of inserted DNA fragment of pBS42 vector was about 2.9 kb and the DNA sequence of the subcloned fragment (Hinc II-Hinc II, 1.4kb) WAS determined. The DNA sequence of ernJ was composed of 357 bp for leader region and 861 bp for the structural gene. Because the leader sequence of ernJ was homologous to that of ermK, the expression of ernJ is also thought to be controlled by a transcriptionl attenuation mechanism.

  • PDF

Characterization of a Chromosomal Nickel Resistance Determinant from Klebsiella oxytoca CCUG 15788

  • Park, Jae-Sun;Lee, Sung-Jae;Rhie, Ho-Gun;Lee, Ho-Sa
    • Journal of Microbiology and Biotechnology
    • /
    • v.18 no.6
    • /
    • pp.1040-1043
    • /
    • 2008
  • Klebsiella oxytoca CCUG 15788 is resistant to $Ni^{2+}$ at a concentration of 10 mM and grows in an inducible manner when exposed to lower concentrations of $Ni^{2+}$. The complete genomic sequence of a 4.2-kb HindIII-digested fragment of this strain was determined from genomic DNA. It was shown to contain four nickel resistance genes (nirA, nirB, nirC, and nirD) encoding transporter and transmembrane proteins for nickel resistance. When the plasmid pKOHI4, encoding nirABCD, was transformed into Escherichia coli JM109, the cells were able to grow in Tris-buffered mineral medium containing 3 mM nickel. TnphoA'-1 insertion mutants in the four nickel genes nirA, nirB, nirC, and nirD showed nickel sensitivity. The nir genes were heterogeneously expressed in E. coli, suggesting functional roles of these genes in nickel resistance.

Properties of an R plasmid pKU41 from pseudomonas putida KU190 (Pseudomonas putida KU 190에서 분리한 R plasmid pKU 41의 특성)

  • 주미자;이영록
    • Korean Journal of Microbiology
    • /
    • v.25 no.2
    • /
    • pp.87-93
    • /
    • 1987
  • The properties of the plasmid pKU41 isolated from Pseudomonas putkda KU190 have been investigated, pKU41 was defined as an R plasmid having a transmissible ampicillin and tetracycline resistance determinant, and could be classified as a plasmid belonging to IncP-1 group according to incompatibility grouping.

  • PDF