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Physiological Changes of Juvenile Abalone, Haliotis sieboldii Exposed to Acute Water-temperature Stress (급격한 수온 스트레스에 따른 시볼트전복, Haliotis sieboldii 치패의 생리적 변화)

  • Kim Tae-Hyung;Kim Kyung-Ju;Choe Mi-Kyung;Yeo In-Kyu
    • Journal of Aquaculture
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    • v.19 no.2
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    • pp.77-83
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    • 2006
  • This study was conducted to investigate changes of hemolymph count, antioxidant enzyme activities (catalase: CAT and superoxide dismutase: SOD) and Heat Shock Protein 70 (HSP70) mRNA in hemolymph, hepatopancreas and gill of abalone (Haliotis sieboldii) exposed to various water temperatures. Abalones were exposed to 10, 15, 20, 25 or $30^{\circ}C$ for 0, 6, 12, 24 or 48 hours. Survival rate of abalone was 100% at 10, 15, 20 and $25^{\circ}C$, but 0% at $30^{\circ}C$. Hemolymph counts increased at lower water temperatures (10 and $15^{\circ}C$) and decreased at $30^{\circ}C$. SOD activity decreased immediately after exposure to lower or higher water temperatures compared to the control ($20^{\circ}C$) with an exception at $30^{\circ}C$ where the activity increased. At lower temperatures, SOD activity rose high after 24 hours, but decreased again at 48 hours. At $25^{\circ}C$, it decreased compared to the control. CAT activity decreased immediately after exposure to 10 or $25^{\circ}C$ compared to the control, and then was recovered to the initial level after increment. At $15^{\circ}C$, CAT activity was high after 6 hours, and then was recovered to the initial level after increment. At $30^{\circ}C$, the activity decreased throughout the experiment. The HSP70 mRNA expression in gill increased at lower temperatures compared to the control ($20^{\circ}C$) and $25^{\circ}C$. In this study, rapid change of wale, temperature caused stress response in abalone which had been raised at $20^{\circ}C$. At molecular level, HSP70 was expressed rapidly, but antioxidant enzymes like SOD and CAT were expressed later than HSP70. At 15 and $25^{\circ}C$ of water temperatures, the HSP70, SOD and CAT expression were stable with time. However, at $30^{\circ}C$, all abalone died possibly because they could not develop resistance to high temperature.

A Study on the Competencies of Automotive Professional Engineers in Korea (자동차 신제품개발 관련 차량기술사의 전문적 업무역량 분석)

  • Kim, Joo-Young;Lim, Se-Yung
    • 대한공업교육학회지
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    • v.33 no.2
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    • pp.192-217
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    • 2008
  • This paper investigated the perceived criticalities and patterns of Korean Professional Engineer's competency regarding the working activities of automative product development, manufacturing, etc by using questionnaires responded to the survey which were applied to the automotive professors, experts and professional engineers (vocational parties) by e/mail, etc. This research investigated the following questions: First, what are the characteristic patterns, relevancy and perceived criticalities of Korean Professional Engineer's competencies? Second, What are the ranked priority of the Korean Professional Engineers' competencies? Are there any differency for each item, sub group of job, intelectual criterior of the competencies between relevancy and perceived criticalities according to the types of vocational parties, etc.? Accoring to the results; first, Professor group showed highest points among 3 groups per each item of the competencies by vocational parties Second, Chassis design group ranked top position among the 8 sub groups by vocational parties and, third, Problem Solving Knowledge ranked highest points than any others. Korean Professional Engineers are found to be positioned as key members, leaders and managers on surveying market, product planning, designing product & components, developing component parts, establishing shop with production equipment, managing quality control & material handling, organizing relevant meetings, developing human resources by training and learning, to back up finance with law matters, cooperating with concerned parties to achieve organizational goals, and to coordinate projects. etc, identifying ethical issues and business skills in order to survive and win to be competitive in various kinds of the automotive industry battle fields.

Analysis of a Principal's Cognition on his Job Performance in Meister High Schools (마이스터고등학교 교장의 직무수행에 대한 교장의 인식분석)

  • Hyun, Su;Kim, Jinsoo
    • 대한공업교육학회지
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    • v.38 no.2
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    • pp.27-47
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    • 2013
  • This study aims to suggest a way to improve the professional abilities of the principals of meister high schools by analysing their perception of the standards of performance of their duties. To carry out this study, we have developed the standards of performance of the duties of the principals of meister high schools through the systematic research on the realm of the duties of the principals of meister high schools, and analysed the principals' perception of their duties using the developed standards of performance of their duties. The results of this study are as followed. First, In the stage of planning the school management, the school management plan, the ways to get budget, the plan for evaluation and feedback of the accomplishment of the performance of school management plan should be included. Second, The area of the school management is subdivided into securing school budget, reviewing and arranging the budgets demanded by teachers, executing school budgets, obtaining school equipments and facilities, maintaining school equipments and facilities, managing school feeding, organizing and operating school steering committee, reflecting the opinions of school steering committee. Third, The school curriculum should be developed by a job analysis and the teaching should reflect the analysis. Fourth, The area of the career path and the management of the career for young meisters includes the analysis of the demand of students, parents, and companies, the development of meister growth route and program for managing their career, and the analysis of meister growth route and reflection of the results of the analysis. Fifth, The field of guiding students includes supporting the students counseling service, and managing a variety of school events. 6th, In the realm of cooperation with communities are included designing programs for collaborating and training students with companies, building cooperation with companies, and obtaining supports from communities and related organizations. 7th, In the area of supporting teachers to improve their professional competence, it figured out that supporting teachers' voluntary learning and studying should take a top priority. In conclusion, it is necessary for meister high school principals to have capability to deal with meister growth route and career management, supporting collaboration with other organizations, building and managing laboratories, encouraging teachers' professional improvement, and operating school curriculum and teaching activities.

Research about a successful adopting for the CRM in the companies (기업에서의 성공적인 CRM 정착에 대한 연구)

  • Kim, Gipyoung
    • The Journal of Industrial Distribution & Business
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    • v.2 no.1
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    • pp.5-15
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    • 2011
  • Prior to the introduction of the CRM, we need to analyze the characteristics and the situations of the company, and should establish a clear vision of the CRM. And each company should identify elements and technologies for introducing the most suitable CRM for them, and optimize them, with long-term perspective. In addition, it requires the implementation strategy which integrates the existing company's routine marketing activities with the concept of the CRM. According to the implementation strategy, the company should improve the business process which is the most effective in investment step by step, and the information system strategy, which develops system investment gradually, should harmonize with it. First, we recognized that raising the company value is important by maximizing customer lifetime value (LTV) by understanding customer needs, and achieving the company's goal through customer satisfaction. Second, we understood that adopting the CRM should be accompanied by changes in the structure, business process and customer contact channels, and it can be successfully integrated with business when it gets proper understandings and attentions of the management. Third, the reality is that there are few cases of successful implementation of domestic companies, and some companies that successfully implement the system mean nothing but implement the solution for developing the CRM. Therefore, it needs to be observed for the long haul, and it seems that we need to approach more systematically to implementation cases for each industry about implementation of the CRM. Fourth, the CRM is no longer the preserve of major companies, and it is the time that medium and small sized enterprises also need it. Taking lesson from Switzerland's small size store merchants who successfully adopt right size of the CRM for their business, for domestic medium and small sized enterprises, the necessity to develop business through developing the CRM models which fit their situations and maintaining relationships with customers has been grown. Fifth, for adopting the CRM business processes, changing or converting the CRM system to the model which fits the company's situation is important rather than applying the advanced company's CRM system model. In other words, the CRM solution which can maximize their own strength by developing the CRM program that makes the most of features and characteristics of the company should be adopted.

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Effects of YK-209 Mulberry Leaves on Disaccharidase Activites of Small Intestine and Blood Glucose-Lowering in Streptozotocin-Induced Diabetic Rats (YK-209뽕잎이 Streptozotocin 유발 당뇨쥐 소장의 이당류분해 효소 활성과 혈당강하에 미치는 영향)

  • 유수경;김미지;김진원;이순재
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.6
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    • pp.1071-1077
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    • 2002
  • The purpose of this study was investigated the effects of YK-209 mulberry leaves on disaccharidase activites of small intestine and blood glucose-lowering in diabetic rats induced with streptozotocin (STZ). Male Sprague-Dawley rats weighing 100$\pm$10 g were randomly assigned to one normal and four STZ-induced diabetic groups; YK-209 0% mulberry leaves diet (DM group),0.1% YK-209 mulberry loaves diet (DM-0.1Y group),0.2% YK-209mulberry leaves diet (DM -0.2Y group), and 0.4% YK-209 mulberry leaves diet (DM -0.4Y group). Diabetes was induced by intravenous injection of 55 mg/kg body weight of STZ in sodium citrate buffer (pH 4.3) via tail vein after 3 weeks feeding of experimental diets. Rats were sacrificed at the 9th day of diabetic states. The functional ingredients in the mulberry leaves, the 1-deoxynojirimycin (DNJ) contents of YK-209 mulberry leaves was higher than those of the Cheongil mulberry leaves. ${\gamma}$ -Aminobutyric acid (GABA) and rutin contents of YK-209 mulberry leaves were 1.3 and 1.4 times higher than those of the Cheongil mulberry leaves, respectively, and vitamin C contents of YK-209 mulberry leaves were also higher than those of the Cheongil mulberry leaves. Intestine index was increased in all diabetic groups, compared with normal group but not significantly different among all diadetic groups. Level of blood glucose was decreased in diadetic rats by supplementation YK-209 mulberry leaves. The disaccharidase activities in proximal part of intestine such as maltase, sucrase, and lactase in YK-209 mulberry leaves supplementation groups were significantly lower than those of DM group, In conclusion, this research indicated that the functional ingredients of YK 209 mulberry leaves were higher than those of the Cheongil leaveses, and YK-209 mulberry leaves has the hypoglycemic effect in STZ-induced diabetic rats.

Study on Anti-inflammatory and Anti-microbial Effect of Pinus rigida Mill. inner Bark Extracts as a Cosmetic Material (리기다소나무(Pinus rigida Mill.) 내수피 추출물에 대한 화장품 소재로써의 항염 및 항균효과)

  • Jang, Min-Jung;Kim, Young-Hun;An, Bong-Jeun;Lee, Chang-Eeon;Lee, Jin-Tae;Kim, Sea-Hyun;Lee, Byung-Guen;Lee, Do-Hyung
    • Journal of Korean Society of Forest Science
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    • v.97 no.3
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    • pp.215-220
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    • 2008
  • Recently, there has been a great deal of interest in the applications of plant-based extracts to both cosmetic and medicinal industries. The objective of this study was to investigate the anti-inflammatory and antimicrobial effect of P. rigida extracts by water and ethyl acetate. Anti-inflammatory and anti-microbial effect of P. rigida extracts by water and EtOAc were investigated by using nitrite scavenging ability, nitric oxide production and anti-microbial ability. In the test of nitrite scavenging ability, P. rigida extracts by water and EtOAc showed 88.7% and 99% at 100 ppm concentration, respectively. The cell viability was measured using the MTT assay at 24 hours after P. rigida extracts as shown in over 80%. Anti-inflammatory effect was examined in LPS stimulated RAW 264.7 cells. NO productions in LPS and P. rigida extracts stimulated group were decreased in a concentration and were dependent on time as compared with LPS stimulated. The water extracts showed the highest inhibition at the 100 ppm concentration. In anti-microbial activity test, the water extract with 3.0 mg/disc resulted in the clear zone of 14 mm, and ethyl acetate with that of 15 mm for Staphylococcus aureus. However, P. rigida extracts didn't show any growth inhibitory effect on Esherichia coli. These results indicate that the extracts of P. rigida have anti-inflammatory activities as a cosmeceuticals.

Comparative Study on the Nurses' Job Satisfaction between the Oriental Medicine Ward and the General Ward (한방병동과 일반병동 간호사의 직무만족도의 비교연구)

  • Byun, Chang-Ja;Choi, Sang-Soon;Paik, Seung-Nam;Lee, Mi-Aie
    • Journal of Korean Academy of Nursing Administration
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    • v.1 no.1
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    • pp.97-111
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    • 1995
  • In our society today, a variety of medical caring system, along with a scientific development in the area of oriental medical science plus national demand, has increased the augmentation and the opening of oriental wards and hospitals (Han Bang), which has come to create an additional requirement of nursing activity at oriental medicine wards should be different from that of the general wards or the same as the other. In view of this, various studies need to be made in this connection. The purpose of this study is to comprehend the number of nurses who want to work at oriental medicine wards and measure job satisfaction in nursing as compared with those working at general wards so as to provide basic materials for future assignment and supplementary training for the nurses. An attempt was made to contribute toward nursing administration on the one hand improving nursing training course on the other hand. A total of 72 nurses currently working at two oriental medicine hospitals available in Seoul and 82 general nurses were selected for this study using the questionaire from December 1993 to January 1994. An instrument for the study was based on the measurement of work satisfaction developed by Paula L. Stamps including 37 questions complemented by Kim for revision (1993) and 14 questions regarding general characteristics and oriental medicine wards. The instrument to test dependability showed Cronbach's=0.7711. The collected data have been processed by computer package SPSS. General characteristics of the two groups and the matters involved in oriental medicine wards were calculated into real figure and percentage an similarities between the two groups were analyzed by t-test and F-test according to the characteristics of variables. The comparative test on work satisfaction among the two groups including general characteristics and work factors were conducted by t-test and F-test. The major findings as a result of the study are as follows : 1. As general characteristics, age group of $26{\sim}30$ years are more than any segment of age. As to marital status, the number of those in single status is slightly higher than the married. Approximately 80% of them are graduates of nursing schools and nursing colleges. They are mostly in service for one to three years. There is no significant difference between the two groups. 2. The number of those who want to work at oriental medicine wards represents 40.3% against 58.4%, being in favor of general wards. 3. The reason for service at oriental medicine wards is that "there is room for potential research" which happened to rank first, followed by "easy job," "good working atmosphere" and "growing interest in oriental medicine." 4. Work satisfactions among nurses who work at oriental medicine wards prove greater than that of nurses who work at general wards. 5. Work satisfaction between the two groups by work factors is reflected with significant difference statistically on task requirement, interaction and doctor-nurse relationships. 6. The general charcateristics and the work satisfaction by work factors prove that there are significant difference in age, marital status, education and the period of work. They tend to be more satisfied with the work as they grow in age. Significant differneces are found in the work factors such as autonomy, adiministration and professional job in the relationship with doctor-nurse. As to marital status, the married are more satisfied than the unmarried. There are significant differneces in the factors respecting administration and doctor-nurse college graduates are highly satisfied with task requirement. However, satisfaction with the professional level has proven the highest degree for those having master degree. The period of work and satisfaction : There are significant differneces in task requirement, administration, interactions, professional level and doctor-nurse relationships. As a general rule, the degree of satisfactions is in proportion to the lengrh of service. The following conclusions are drawn based on the fndings mentioned above. Even though the work satisfaction of the nurse who work at oriental medicine ward is relatively high, it is desired that personal consultation be given as to disposition of nurses when they are assigned to oriental medicine wards. It is also recommended that lectures on oriental medicine be conducted through supplementary training and/or basic nursing course in order that they may be motivated for ingenious activities with an increasing sense of self-esteem which will eventually enhance positive changes for the patients who are in need of oriental medicine nursing and for the medical teams. In addition, joint reseaches involvingclinical care and education should be in constant process for unique and scientific development for those who are subject to oriental medicine nursing care.

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A Study on tile Presumption of Chang Pogo's Maritime Routes (해상왕 장보고의 해상항로 추정에 관한 연구)

  • 김형근
    • Journal of the Korean Institute of Navigation
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    • v.25 no.1
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    • pp.77-91
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    • 2001
  • According to the many ancient literatures, it is said to be that Merchant Prince, Chang Pogo in unification Silla had traded with not only China, Japan but also Arab-Islam Countries, but unfortunately there is no definite records about this. Therefore in this study the writer would like to presume the sea routes of Merchant Prince, Chang Pogo in a unification Silla. For the purpose of this study the writer would like to study the reference books and materials regarding to the sea routes of prehistoric age and ancient three countries of Kingdoms and unification Silla. Especially the writer consider “The Work of Routes and Kingdoms” of Ibn Khurdarhbih regarding to the ancient sea routes of Korea, China, Arab-Islam Countries, and also the writer consider the ancient sea routes of Tang Dynasty in China and Ryukyu Kingdom in Japan. About this methodology of this study, the writer believe that the sea routes of today is the results of ancient sea routes, and this ancient sea routes could not be changed forever except special circumstance. According in this study the writer would like to persume the sea routes of Merchant Prince, Chang Pogo using like this methodologies. This presumption of the writer is originated on the basis of The Work of Routes and Kingdoms written by ancient Arabian Geographer, Ibn Khurdadhbih in the early years of 9th century. The Work of Routes and Kingdoms is geography, and the principal contents of this geography are the activities of Arab-Islam merchants and Chinese Merchants between Arab-Islam countries and China from 9th century to 11th century by ancient sea routes. But surprisingly in this geography, Silla was marked, and another ancient Arabian geogrpher, Al-Biruni marked Silla on his map, Qanun Al-mas'udi in the 9th century, and also another ancient Arabian geographer, Al-Idrisi marked Silla on his map in the 11th century. Especially Al-Biruni expressed the longitude and latitude of Silla on his map, he expressed Silla as following. “at the extreme end of China toward the East and few people travelled to it by sea”. Therefore the writer would like to presume that Arab-Islam merchants had traded with unification Silla in the 9th century, and also Merchant Prince, Chang Pogo had traded with not only China, Japan but also Arab-Islam Countries using above ancient sea routes in the 9th century.

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The Interpretation for Stand Structure in Natural Oak Forests (참나무 천연림(天然林)의 임분(林分) 구조(構造)에 대한 해석(解析))

  • Kim, Ji Hong;Lee, Don Koo;Kim, Zin-Suh;Lee, Kyung Joon;Hyun, Jung Oh;Hwang, Jae Woo;Kwon, Ki Won
    • Journal of Korean Society of Forest Science
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    • v.82 no.3
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    • pp.235-245
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    • 1993
  • This study was carried out to understand the stand structure based on the compositional characteristics of natural deciduous forest dominated by Quercus species, which will provide with fundamental ecological and silvicultural information for effective forest management. Of two tracts selected from the Seoul National University Research Forests, one is located in Kwangju-Gun Kyunggi-Do, and the other in Kwangyang-Gun Chollanam-Do. Point-quarter sampling method w-as employed to survey woody vegetation confined to larger than 6cm in DBH. The numbers of sample points were 152 and 187, in Kwangju-Gun and Kwangyang-Gun, respectively. Comparative analysis for two forest tracts was made as follows ; 1) species composition by calculation of importance values ; 2) number of stems per hectare for major tree species ; 3) species diversity for the forests in two locations ; 4) inter-species association based on $X^2$ test. The relative dominate of Quercus speices showed 88% at Kwangju-Gun, Kyunggi-Do and 50% at Kwangy-ang-Gun. Chollanam-Do. Such high dominance occurred due to high importance values of Quercus variahilis and Q. mortgolica in both locations. Quercus serrata showed low value at Kwangju-Gun. Kyunggi-Do whereas Q. acutissirna, Q. dentata and Q. aliena did none at Kwangyang-Gun. Chollanam-Do. The species diversity and evenness in oak communities exhibited 0.82 and 0.61, respectively at Kwangju-Gun. Kyunggi-Do, while those did 1.09 and 0.73 at Kwangyang-Gun, Chollanam-Do. Therefore, it was considered that Ku-angju-Gun region was disturbed severely by human activities but Kwangyang-Gun region eras less disturbed. Frequent appearance of Carpinus and Acer species indicates increase in diversities and better development of succession at Kwangyang-Gun region.

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Studies on Quality Changes and Antioxidant Activity During the Fermentation of the Salt Fermented Whangseoke (황석어(Collichthys nireatus Jordan et starks) 젓갈의 숙성과정 중 품질변화와 항산화작용에 관한 연구)

  • Kim, Ji-Sang;Moon, Gap-Soon;Lee, Kyung-Hee;Lee, Young-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.2
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    • pp.171-176
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    • 2006
  • The effect of storage temperature on the quality and antioxidative activity Whangseoke sauce was studied over a period of 240 days. Fermented Whangseoke with $25\%$ salt were stored at $25^{\circ}C$. The quality change and antioxidant activity of Whangseoke in linoleic acid emulsion was evaluated with various parameters including acids values, peroxide values, TBA values, reducing sugar, brown color intensity, electron donating ability and reducing power at various time intervals for 240 days of storage. In general, it was observed, in all sample, that peroxide values, brown color intensity, electron donating ability and reducing power gradually increased, while reducing sugar decreased during storage at $25^{\circ}C$. The antioxidative activities of fermented Whangseoke were determined on tile linoleic acid emulsion system. The results showed that Whangseoke had antioxidant activity. These results suggest that antioxidant activity of Whangseoke seemed to influence by Maillard reaction products during the storage periods.