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Inhibitory Effects of Phellinus linteus and Rice with Phellinus linteus Mycelium on Obesity and Diabetes (상황버섯, 상황버섯균사체배양쌀 추출물의 비만 및 당뇨 억제 효과)

  • Kim, Haeseop;You, Jeheon;Jo, Yeongcheol;Lee, Youngjae;Park, Inbae;Park, Jeongwook;Jung, Myung-A;Kim, Young-Suk;Kim, Sunoh
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.7
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    • pp.1029-1035
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    • 2013
  • The objective of this study was to examine the ability of extracts from Phellinus linteus (PL) and rice with Phellinus linteus mycelium (PLM) to inhibit obesity and diabetes. The efficacy of PL and PLM were evaluated using Oil Red O staining, cholesteryl ester transfer protein (CETP) levels, protein tyrosine phosphate 1B (PTP1B) levels, organ weight, and serum lipid levels. Lipid accumulation significantly decreased by 76% and 59% upon treatment with $300{\mu}g/mL$ of PL and PLM, respectively (P<0.01). The inhibition of CETP activity increased 99% upon treatment with $300{\mu}g/mL$ of PL or PLM. Treatment with 3, 10, 30, 100, and $300{\mu}g/mL$ of PL, changed PTP1B activity by 10, 11, 14, 12, and 18% respectively. Also, treatment with increasing concentrations of PLM led to a significant concentration-dependent inhibition of PTP1B activity (P<0.01). PL and PLM were orally administered for 28 days after a high fat diet (HFD). PL significantly (P<0.05) reduced triglyceride and cholesterol levels. In addition, PLM significantly (P<0.05) reduced triglyceride, cholesterol, and HDL-cholesterol levels. GOT and GPT were not significantly affected. These results indicate that PL and PLM extracts have potent and useful activities for the treatment of obesity and diabetes mellitus.

Biogeochemical Studies on Tidal Flats in the Kyunggi Bay: Introduction (경기만 부근 갯벌의 생지화학적 연구: 서문)

  • Cho, B.C.;Choi, J.K.;Lee, T.S.;An, S.;Hyun, J.H.
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.10 no.1
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    • pp.1-7
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    • 2005
  • Tidal flats have been regarded to carry out transformation and removal of land-derived organic matter, and this purifying capability of organic matter by tidal flats is one of very important reasons for their conservation. However, integral biogeochemical studies on production and decomposition of organic matter by benthic microbes in tidal flats have been absent in Korea, although the information is indispensable to quantification of the purifying capability. Our major goals in this multidisciplinary research were to understand major biogeochemical processes and rates mediated by diverse groups of microbes dominating material cycles in the tidal flats, and to assess the contribution of benthic microbes to removal of organic matter and nutrients in the tidal flats. Our study sites were Ganghwa and Incheon north-port tidal flats that had been regarded as naturally well reserved and organically polluted, respectively. Our research group measured over 3 years primary production, biomass and community structure of primary producers, abundance and production of bacteria, enzyme activities, distribution of protozoa and protozoan grazing rates, rates of denitrification and sulfate reduction, early sediment diagenesis, primary production and respiration based on oxygen microelectrode. We analyzed major features of each biogeochemical process and their interactions. The results are compiled in the following articles in this special issue: An (2005), Hwang and Cho (2005), Mok et at. (2005), Na and Lee (2005), Yang et at. (2005), and Yoo and Choi (2005).

Distribution Characteristics of Polycyclic Aromatic Hydrocarbons(PAHs) in Riverine Waters of Ulsan Coast, Korea (울산연안 하천에서 다환방향족탄화수소 분포특성)

  • You, Young-Seok;Lee, Jeong-Hoon;Park, Jeong-Chae;Kim, Dong-Myung;Cho, Hyeon-Seo
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.18 no.5
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    • pp.398-405
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    • 2012
  • PAHs(Polycyclic Aromatic Hydrocarbons) in coastal area and estuary adjacent to urban area and industrial activities region are mainly introduced into marine environment via atmosphere and river. This study which is evaluated the distribution characteristics of PAHs discharge from rivers and sewage disposal water which flowing into Ulsan bay, and were carried out in Jun of dry season and in Auguest of wet season, 2008. The water samples from eight main rivers and Youngyeon WWTP(Wastewater Treatment Plant) flowed into Ulsan bay were taken for analysis of dissolved PAHs. The range and mean values of dissolved PAHs concentrations showed 10.30 to 87.88 ng/L, its mean 36.94 ng/L in dry season and 10.30 to 69.57 ng/L, its mean 24.37 ng/L in wet season. The distribution of dissolved PAHs showd the high concentrations in the Gungcheoncheon which is flowed from urban and industrial area. The ranges and means values of the loading fluxes were estimated with 0.04 to 8.27 g/day, its mean 2.05 g/day in dry season, and 0.03 to 4.77 g/day, its mean 1.61 g/day, in wet season. The loading flux showed the highest value in Taewha river due to the high flow rate and the urban activity. The composition patterns of dissolved PAHs compound showed most of the trend occupying low molecuar weight PAHs than high molecular weight PAHs. These results were due to physical and chemical characteristics of PAHs compound, and were similar to those of other studies. The concentrations of dissolved PAHs in this study are lower than those of other studies, and the level of PAHs pollution appeared to be not serious in reverine waters of Ulsan coast.

A Study on awareness of environmental management Knowledge and enviromental management behavior of Housewives (주부의 환경관리지식 인지도와 환경관리행동에 관한 연구)

  • 노남숙
    • Hwankyungkyoyuk
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    • v.7 no.1
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    • pp.106-122
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    • 1994
  • The purpose of this study to investigate the awareness of environmental management knowledge and behavior and the effect of independent variables (The environmental management behavior of housewives are composed of five activities-clothing, eating, housing, buying, family management. The independent variables are composed of objective variable and subjective variable). The Research Questions are as follows; 1. Are there significant differences in the housewife's awareness of the environmental management knowledge according to the objective variable and the subjective variable? 2. How much are the effects of related variables on the awareness of environmental management knowledge? 3. Are there significant differences in the housewife's environmental management behavior according to objective variable and subjective variable and awareness of environmental management knowledge? 4. How much are effects of related variables on the housewife's environmental management behavior? For these purpose, a theoretical framework was developed by literature review and applied to empirical test. The data was collected through the questionnaire those respondents were 477 housewives in seoul. It was analyzed by various statistical methods such as Frequency. Percentile, GLM, F-test, T-test, Duncan's Multiple Range Test, Pearson's Correlations Analysis Simple Regression Analysis, and Multiple Regression Analysis. The results of this research are as follows; 1. The awareness of environmental management knowledge had significant differences according to the variables such as housewife's age, husband's job, family number, education experience of environment, frequencies of contracting mass media, the perceived adequacy of resources, social-altruistic orientation. 2. Among all of these variables affecting the awareness of environmental management knowledge, the perceived adequacy of resources had the highest effects and the next were family number, social-altruistic, type of residence in order. 3. Total Environmental management behavior of housewives had significant differences according to the variables such of environment, frequencies of contracting mass media, the satisfaction of household labor, the perceived adequacy of resources, biospheric orientation, social-altruistic orientation, egoism orientation, the awareness of environmental management knowledge. The clothing, eating, housing, family management behavior had the high level, the buying management behavior had the average level. The clothing, eating, housing, buying, family management behavior had significant differences according to the variables such as the satisfaction of household labor, the perceived adequacy of resources, biospheric orientation, social-altruistic orientation, egoism orientation, the awareness of environmental management knowledge. In particular, the clothing, eating, housing, buying management behavior had significant differences according to the variables such as education experience of environment. And the housing, buying management behavior had significant differences according to the variables such as frequencies of contracting mass media. 4. Among all of theses variables affecting environmental management behavior, the most influence variables was awareness of environmental management knowledge and the next were influence of housewife's job, biospheric orientation, the satisfaction of household labor, education experience of environment, frequencies of contraction mass media, egoism orientation in order. Among all of theses variables effecting the clothing, eating, housing, buying management behavior the variables affecting the family management behavior, the most influences variables was housewife's job. In the result of analysing the housewife's environmental management behavior and awareness of environmental management knowledge, influences of education experience of environment had the highest effect and frequencies of contracting mass media may more influencial than other variables. And Concrete and systematic knowledge and technique of environmental education is requisite. The result of this study could be attributed to develop the efficient environmental information. More emphasis should be given to environmental education and a long range environmental policy. in particular, environmental education be effectively related to environmental behavior, life-education and systematic support must be provided. In addition, further study of environmental problem is needed for and application of various theoretical approaches and research method.

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Fate of Heavy Metals in Activated Sludge: Sorption of Heavy Metal ions by Nocardia amarae

  • Kim, Dong-wook
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 1998.10a
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    • pp.2-4
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    • 1998
  • Proliferation of Nocardia amarae cells in activated sludge has often been associated with the generation of nuisance foams. Despite intense research activities in recent years to examine the causes and control of Nocardia foaming in activated sludge, the foaming continued to persist throughout the activated sludge treatment plants in United States. In addition to causing various operational problems to treatment processes, the presence of Nocardia may have secondary effects on the fate of heavy metals that are not well known. For example, for treatment plants facing more stringent metal removal requirements, potential metal removal by Nocardia cells in foaming activated sludge would be a welcome secondary effect. In contrast, with new viosolid disposal regulations in place (Code o( Federal Regulation No. 503), higher concentration of metals in biosolids from foaming activated sludge could create management problems. The goal of this research was to investigate the metal sorption property of Nocardia amarae cells grown in batch reactors and in chemostat reactors. Specific surface area and metal sorption characteristics of N. amarae cells harvested at various growth stages were compared. Three metals examined in this study were copper, cadmium and nickel. Nocardia amarae strain (SRWTP isolate) used in this study was obtained from the University of California at Berkeley. The pure culture was grown in 4L batch reactor containing mineral salt medium with sodium acetate as the sole carbon source. In order to quantify the sorption of heavy metal ions to N amarae cell surfaces, cells from the batch reactor were harvested, washed, and suspended in 30mL centrifuge tubes. Metal sorption studies were conducted at pH 7.0 and ionlc strength of 10-2M. The sorption Isotherm showed that the cells harvested from the stationary and endogenous growth phase exhibited significantly higher metal sorption capacity than the cells from the exponential phase. The sequence of preferential uptake of metals by N. amarae cells was Cu>Cd>Ni. The specific surFace area of Nocardia cells was determined by a dye adsorption method. N.amarae cells growing at ewponential phase had significantly less specific surface area than that of stationary phase, indicating that the lower metal sorption capacity of Nocardia cells growing at exponential phase may be due to the lower specific surface area. The growth conditions of Nocardia cells in continuous culture affect their cell surface properties, thereby governing the adsorption capacity of heavy metal. The comparison of dye sorption isotherms for Nocardia cells growing at various growth rates revealed that the cell surface area increased with increasing sludge age, indicating that the cell surface area is highly dependent on the steady-state growth rate. The highest specific surface area of 199m21g was obtained from N.amarae cell harvested at 0.33 day-1 of growth rate. This result suggests that growth condition not only alters the structure of Nocardia cell wall but also affects the surface area, thus yielding more binding sites of metal removal. After reaching the steady-state condition at dilution rate, metal adsorption isotherms were used to determine the equilibrium distributions of metals between aqueous and Nocardia cell surfaces. The metal sorption capacity of Nocardia biomass harvested from 0.33 day-1 of growth rate was significantly higher than that of cells harvested from 0.5- and 1-day-1 operation, indicatng that N.amarae cells with a lower growth rate have higher sorpion capacity. This result was in close agreement with the trend observed from the batch study. To evaluate the effect of Nocardia cells on the metal binding capacity of activated sludge, specific surface area and metal sorption capacity of the mixture of Nocardia pure cultures and activated sludge biomass were determined by a series of batch experiments. The higher levels of Nocardia cells in the Nocardia-activated sludge samples resulted in the higher specific surface area, explaining the higher metal sorption sites by the mixed luquor samples containing greater amounts on Nocardia cells. The effect of Nocardia cells on the metal sorption capacity of activated sludge was evaluated by spiking an activated sludge sample with various amounts of pre culture Nocardia cells. The results of the Langmuir isotherm model fitted to the metal sorption by various mixtures of Nocardia and activated sludge indicated that the mixture containing higher Nocardia levels had higher metal adsorption capacity than the mixture containing lower Nocardia levels. At Nocardia levels above 100mg/g VSS, the metal sorption capacity of activate sludge increased proportionally with the amount of Noeardia cells present in the mixed liquor, indicating that the presence of Nocardia may increase the viosorption capacity of activated sludge.

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Development of Lotus Root Bugak with Plasma Lipid Reduction Capacity by Addition of Opuntia ficus-indica var. saboten or Green Tea as a Coloring Agent (백년초 및 녹차 가루 첨가 연근 부각의 지질저하 기능성)

  • Kim, Mijeong;Hong, Sun Hee;Chung, Lana;Choe, Eunok;Song, Yeong-Ok
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.3
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    • pp.333-340
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    • 2014
  • The purpose of this study was to develop functional lotus root bugak with plasma lipid reduction capacity by controlling the color of batter used for bugak preparation. Lotus root, nearly colorless, was selected to observe color effects. Gardeniae fructus (GF), Opuntia ficus-indica var. saboten (OF), and green tea (GT), which are colored yellow, red, and green, respectively, were used as coloring agents. Fermented glutinous rice was prepared naturally during winter season by placing glutinous rice and water (1:2, w/w) together in a crock pot for 7 days. Coloring materials (10%, w/w) were blended with glue made from fermented glutinous rice flour to prepare the batter. Cooked lotus root was then mixed with a 1.1-fold amount of batter (w/w) and dried at room temperature. Lotus root bugak (LRB) is pan-fried with un-roasted sesame oil, which is traditionally used as frying oil in Korea. Low-density lipoprotein receptor knockout ($LDLr^{-/-}$) mice (n=36) were fed an atherogenic diet (AD) containing various types of LRB (10 g%) for 10 weeks. Plasma triglyceride, total cholesterol, and LDL-C concentrations decreased significantly in mice fed LRB prepared with OF batter (OFB) and GT batter (GTB) (P<0.05). Protein expression levels of fatty acid synthase (FAS) and 3-hydroxyl-3-methylglutaryl coenzyme A reductase (HMGCR) in the OFB and GTB groups were suppressed compared with the LRB group (P<0.05). In accordance with the results on FAS and HMGCR expression, sterol regulatory element binding protein-I and II (SREBP-I and II), which are responsible for the regulation of FAS and HMGCR gene expression, respectively, were down-regulated compared to the LRB group (P<0.05). In conclusion, the plasma lipid reduction activities of OFB and GTB could be mediated through down-regulation of FAS and HMGCR mRNA expression via suppression of regulatory molecules, SREBP-I and II, in $LDLr^{-/-}$ mice.

The Stakeholder's Response and Future of Mountain Community Development Program in Rep. of Korea (한국 산촌개발사업에 대한 이해관계자의 의식과 향후 발전방안)

  • Yoo, Byoung Il;Kim, So Heui;Seo, Jeong-Weon
    • Journal of Korean Society of Forest Science
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    • v.94 no.4 s.161
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    • pp.214-225
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    • 2005
  • The mountain village development program in Korea started in the mountain villages, the 45.9% of total land and one of the typical marginal region, from 1995 to achieve the equilibrium development of national land and the sustainable mountain development in Chapter 13 in Agenda 21, and it has been accelerated to increase the happiness and the quality of life of mountain community residents through the expansion by province and the improvement of related laws and regulations. This study has been aimed to analyze the response of main stakeholder's -mountain village residents and local government officials - on mountain villages development, and to provide the future plan as community development. The survey and interview data were collected from the mountain villages which already developed 59 villages and developing 15 villages in 2003. The mountain village development program has achieved the positive aspects as community development plan in the several fields, - the voluntary participation of residents, the establishment of self-support spirit as the democratic civilians, the development of base of income increasement, the creation of comfortable living environment, the equilibrium development with the other regions. Especially the mountain residents and local government officials both highly satisfy with the development of base of income increasement and the creation of comfortable living environment which are the main concerns to both stakeholder. However through the mountain development program, it is not satisfied to increase the maintenance of local community and the strengthening of traditional value of mountain villages. Also to improve the sustainable income improvement effects, it is necessary to develop the income items and technical extension which good for the each region. In the decentralization era, it is necessary for local government should have the more active and multilateral activities for these. With this, the introduction of methods which the mountain community people and the local government officials could co-participate in the mountain villages' development from the initial stages and the renovation of related local government organizations and the cooperatives will be much helpful to the substantiality of mountain development program. Also it is essential for the assistance of central government to establish the complex plan and the mountain villages network for all mountain area and the exchange of information, the education and training of mountain villages leader who are the core factor for the developed mountain villages maintenance, the composition of national mountain villages representatives. In case the development proposals which based on the interests of the main stakeholder's on mountain community could be positively accepted, then the possibility of the mountain village development as one of community development will be successfully improved in future.

Study on Public Awareness of establishing Marine Protected Areas - Case Study of Guimaras Province, Philippines using Contingent Valuation Method (해양보호구역의 설정에 대한 대중인식도 연구 - 조건부 가치측정법을 이용한 필리핀 기마라스주의 사례)

  • Kim, Tae-Goun
    • Journal of Navigation and Port Research
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    • v.38 no.6
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    • pp.663-672
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    • 2014
  • In Korea and the Philippines, as well as all over the world, with the recognition of the importance of marine ecological resources, the marine protected areas(MPA) have been established and managed to protect and preserve these resources. While the number of marine protected areas for marine ecological resources protection has been increased, there is main problem that the most of MPAs do not achieve their intended management objectives. the effective management. Because of the positive and negative impacts on local communities and fishermen as direct stockholders, there has been ongoing debate on the pros and cons of implementing MPAs. Accordingly, this research conducted a case study of establishing Marine Protected Areas in Guimaras, Philippines because Philippines fisheries code of 1998 (Republic Act 8550), which is enacted to manage, conserve and protect fishery resources, obliged local governments to designate no less than 15% of jurisdictional municipal water as fisheries resource protection areas for a long time. To do this, a dichotomous-choice contingent-valuation survey was conducted in the two municipalities of Guimaras, Philippines to investigate public opinion in debates over MPAs and to estimate willingness to pay (WTP) for MPAs to protect and conserve marine habitats for fishery resources. Because of the expected economic costs by prohibiting fishing activities within the establishing newMPA, 58.7% of respondents thought the costs should be compensated, but 91.4% respondents voted in favor of increasing MPAs for fisheries resources as a protective measure. Finally, with Contingent Valuation Method(CVM), the aggregate mean WTP (375.5ha) of San Lorenzo and Sibunag residents in Guimaras Province, Philippines for establishing the additional MPA in their municipality waters was estimated to $1,046,791. Therefore, these findings could be used as a valuable data for establishing effective management plan of MPAs in Korea.

Analysis of Morphological Characteristics for Normal Maize Inbred Lines (종실옥수수 자식계통들에 대한 형태적 특성 연구)

  • Park, Jong Yeol;Sa, Kyu Jin;Park, Ki Jin;Lee, Ju Kyong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.3
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    • pp.312-318
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    • 2014
  • We evaluated the morphological characteristics of 156 maize inbred lines, which were developed to breeding normal maize variety at Maize Experiment Station, Gangwon Agricultural Research and Extension Services, by examining 11 quantitative and three qualitative characteristics. On the evaluation of three qualitative traits for 156 maize inbred lines, most inbred lines showed yellow (85 and 84 inbred lines) at tassel color (QL1) and silk color (QL2), and showed semi erect (105 inbred lines) at plant type (QL3). While, the evaluation of 11 quantitative traits for 156 maize inbred lines, they showed the morphological variation in days of tasseling (QN1, 56.5 to 76.0 days), days of silking (QN2, 59.0 to 85.5 days), stem thickness (QN3, 12.7 to 42.9 mm), plant height (QN4, 111.8 to 239.8 cm), ear height (QN5, 48.2 to 126.5 cm), 100 kernel weight (QN6, 14.9 to 36.4 g), ear length (QN7, 10.0 to 79.0 cm), setted kernel length (QN8, 8.0 to 70.5 cm), ear thickness (QN9, 4.0 to 22.0 cm), total kernel weight (QN10, 22.0 to 490.0 kg) and water content (QN11, 9.3 to 11.9%), respectively. As a result, 11 inbred lines (00hf3, 00hf19, 00hf30, 00hf36, 02S8069, 02S8072, 02S8090, 02S8099, 05S10011, 06S8085-6, 07S8011) in the 156 normal maize inbred lines have showed comparatively high values. While, the results of PCA (principal component analysis) indicated that the ear length (QN7), setted kernel length (QN8), ear thickness (QN9) and total kernel weight (QN10) greatly contributed in positive direction on the first principal components. And also, days of tasseling (QN1), days of silking (QN2), plant height (QN4) and ear height (QN5) contributed in negative direction on the second principal component. Thus these morphological characters, which were greatly contributed in the first and second principal components, might be considered to be useful for discrimination among 156 normal inbred lines. Specifically, this study's assessment of morphological characteristics of 156 normal inbred lines will be helpful useful for normal maize breeding programs such activities as planning crosses for hybrid and line development at Maize Experiment Station, Gangwon Agricultural Research and Extension Services.

Effect of Sand Extraction on Meiobenthic Community of Jangbong-do in the Eastern Yellow Sea of Korea (서해 주문도 연안 사질 조하대에서의 해사채취가 중형저서동물 군집에 미치는 영향 연구)

  • Kang, Teawook;Min, Won-Gi;Hong, Jae-Sang;Kim, Dongsung
    • Korean Journal of Environmental Biology
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    • v.32 no.2
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    • pp.138-152
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    • 2014
  • The objective of the study survey was to determine the effect of marine sand extraction on community composition and rate of recolonization of the meiobenthos following cessation of mining activities. Because of meiobenthic distribution in nature, high abundance, intimate association with sediments, fast reproduction, benthic larva period, sensitivity to pollution and rapid life histories, meiobenthos are widely regarded as ideal organisms to study the potential ecological indicator of natural and anthropogenic stresses. The community structure of meiobenthos was studied at seven stations within sandy tidal and sub tidal zones in Jangbongdo in the Yellow Sea, Korea from Aug. 2006 to Dec. 2007. Meiobenthic samples were collected by three core samples, with a 3.6 cm in diameter, from each sediment sample taken with a Smith-McIntyre Grab. It was found that sand mining often causes complete removal of the sediment and the damage to the habitats of meiobenthos. This study in the effect showed that sand mining resulted in a reduction in total abundance and biomass of meiobenthos in mining area. The finding of this study further showed that initial restoration of abundance and biomass within one year of the cessation of sand mining.