• 대한의생명과학회지
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• 제23권1호
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• pp.25-29
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• 2017

This study was to investigate the role of antioxidants on development in arsenite exposed porcine embryos. Oocytes were collected from porcine ovary, and then matured for 44 h. Maturated oocytes were incubated with sperm for 6 h, and fertilized oocytes with sperm (embryos) cultured for 48 h. After, embryos were culture with arsenite and/or antioxidants (melatonin, silymarin, curcumin and vitamin) for 120 h. Formation of pre-morulae, morulae and blastosysts rate was measured using microscope. In results, 10, 100 and 100 nM arsenite significantly decreased morulae and blastocysts formation compared to control in pigs (P<0.05). $10{\mu}M$ silymarin and $100{\mu}M$ vitamin E increased blastocyst formation compared to 10 nM arsenite exposed embryos, but there were no significantly among the treatment, and 1 nM melatonin and $5{\mu}M$ curcumin did not influence blastocysts formation in 10 nM arsenite exposed embryos. In summary, arsenite decreased embryo development, $10{\mu}M$ silymarin, $100{\mu}M$ vitamin E, 1 nM melatonin and $5{\mu}M$ curcumin had no positive effect to blastocyst formation in arsenite exposed porcine embryos. Therefore, we suggest that little arsenite may have negative effect to embryo development, and silymarin, vitamin E, melatonin and curcumin could not rescue embryo development from damage by arsenite in pigs.

• 한국콘텐츠학회논문지
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• 제9권12호
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• pp.270-278
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• 2009

그동안 공무원의 스트레스 요인과 직무만족에 대한 연구는 대부분이 역할 관계와 인간 관계에 따른 스트레스 증가로 인한 직무만족의 저하에 대한 연구가 대부분을 이루고 있었다. 그러나 소방공무원의 경우, 직무상 위험성, 긴급성, 대기성, 교대성의 특징을 지니고 있기 때문에 직무 특성에 따른 스트레스가 다소 발생할 것으로 사료된다. 따라서 본 연구는 소방공무원의 스트레스 요인을 직무특성(위험성, 긴급성, 대기성, 교대성)요인과 조직내역할(역할갈등, 역할모호, 역할과다)요인으로 구분하여 각각 직무만족에 어떠한 영향력을 미치는지를 실증 분석 하였다. 결과적으로 소방공무원의 직무특성 요인과 조직내역할 요인은 직무만족에 유의미한 영향력을 미치고 있었고, 구체적으로 직무특성 요인 중에서는 긴급성과 대기성이 직무만족에 정(+)의 영향력을 미치고 있었고, 조직내역할 요인 중에서는 역할 갈등과 역할 과다가 직무만족에 부(-)의 영향력을 미치고 있는 것으로 결과가 나타났다. 또한 업무형태(화재 진압, 구조 구급, 소방 행정, 기타)에 따라 스트레스 요인 및 직무만족에 유의미한 차이를 보이고 있었고, 근무기간(소방본부, 소방서, 119안전센터)에 따라서도 스트레스 요인 및 직무만족에 유의미한 차이를 보이고 있었다.

• The Korean Journal of Pain
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• 제29권1호
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• pp.34-39
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• 2016

Background: Although opioids are the most commonly used medications to control postoperative pain in children, the analgesic effects could have a large inter-individual variability according to genotypes. The aim of this study was to investigate the association between single nucleotide polymorphisms and the analgesic effect of morphine for postoperative pain in children. Methods: A prospective study was conducted in 88 healthy children undergoing tonsillectomy, who received morphine during the operation. The postoperative pain score, frequency of rescue analgesics, and side effects of morphine were assessed in the post-anesthesia care unit. The children were genotyped for OPRM1 A118G, ABCB1 C3435T, and COMT Val158Met. Results: Children with at least one G allele for OPRM1 (AG/GG) had higher postoperative pain scores compared with those with the AA genotype at the time of discharge from the post-anesthesia care unit (P = 0.025). Other recovery profiles were not significantly different between the two groups. There was no significant relationship between genotypes and postoperative pain scores in analysis of ABCB1 and COMT polymorphisms. Conclusions: Genetic polymorphism at OPRM1 A118G, but not at ABCB1 C3435T and COMT Val158Met, influences the analgesic effect of morphine for immediate acute postoperative pain in children.

• The Korean Journal of Physiology and Pharmacology
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• 제21권5호
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• pp.495-507
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• 2017

The effect of clonidine administered intrathecally (i.t.) on the mortality and the blood glucose level induced by sepsis was examined in mice. To produce sepsis, the mixture of D-galactosamine (GaLN; 0.6 g/10 ml)/lipopolysaccharide (LPS; $27{\mu}g/27{\mu}l$) was treated intraperitoneally (i.p.). The i.t. pretreatment with clonidine ($5{\mu}g/5{\mu}l$) increased the blood glucose level and attenuated mortality induced by sepsis in a dose-dependent manner. The i.t. post-treatment with clonidine up to 3 h caused an elevation of the blood glucose level and protected sepsis-induced mortality, whereas clonidine post-treated at 6, 9, or 12 h did not affect. The pre-treatment with oral D-glucose for 30 min prior to i.t. post-treatment (6 h) with clonidine did not rescue sepsis-induced mortality. In addition, i.t. pretreatment with pertussis toxin (PTX) reduced clonidine-induced protection against mortality and clonidine-induced hyperglycemia, suggesting that protective effect against sepsis-induced mortality seems to be mediated via activating PTX-sensitive G-proteins in the spinal cord. Moreover, pretreatment with clonidine attenuated the plasma tumor necrosis factor ${\alpha}$ ($TNF-{\alpha}$) induced by sepsis. Clonidine administered i.t. or i.p. increased $p-AMPK{\alpha}1$ and $p-AMPK{\alpha}2$, but decreased p-Tyk2 and p-mTOR levels in both control and sepsis groups, suggesting that the up-regulations of $p-AMPK{\alpha}1$ and $p-AMPK{\alpha}2$, or down-regulations of p-mTOR and p-Tyk2 may play critical roles for the protective effect of clonidine against sepsis-induced mortality.

• 디지털융복합연구
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• 제12권10호
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• pp.415-423
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• 2014

본 연구는 보건계열 대학생을 대상으로 일반적 특성에 따른 학과만족도, 진로의사결정유형, 진로준비행동의 차이를 파악하고, 변수들 간의 관계와 진로준비행동에 미치는 영향을 파악하여, 진로지도 프로그램을 개발하는데 기초자료를 제공하고자 시행하였다. 연구의 대상자는 B와 G광역시에 소재한 3년제 학과인 응급구조과, 물리치료과, 보건행정과, 치위생과 대학생을 임의선정하고 편의표집 한 450명으로 하였다. 조사기간은 2013년 11월 12일부터 15일까지이었고, 배부된 설문지 450부 중 418부를 최종 분석하였다. 자료분석은 SPSS 14.0 for Window 프로그램을 사용하였다. 연구결과, 변수들 간의 상관관계를 보였고, 학과만족도는 진로준비행동에 가장 큰 영향은 적성학습이었고, 다음으로 대인관계, 진로직업이었다. 진로의사결정유형의 합리적 유형은 진로준비행동에 정(+)의 영향을 보였고, 의존적 유형, 직관적 유형은 진로준비행동에 부(-)의 영향을 보였다.

• 한국컴퓨터정보학회논문지
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• 제23권1호
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• pp.103-109
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• 2018

The purpose of the study is to investigate the effect of a simulation training of BLS in paramedics in pre-hospital situation. This a nonequivalence control quasi-experimental study. The study subjects were 8 paramedics of experimental group and 8 paramedics of control group in K fire department. An informed consent was written by the subjects after explaining of the purpose of the study. The study methods consisted of conventional education and practice training. The conventional education was done for 30 minutes and the practice training was taken by four trainees of one group and the instructor demonstrated Basic Iife Support (BLS) performance for three minutes. Each trainer peformed BLS for ten minutes. In the beginning of the course, two paramedics got off from the ambulance and performed BLS including 5 cycles of Cardiopulmonary Resuscitation (CPR). Soon after the BLS, another two paramedics performed pre-hospital BLS survey. The education was guided by two professors of emergency medical technology, two Basic Iife Support instructors, and two emergency rescue directors. Pre-hospital BLS was measured by a 5-point Likert scale. Higher score means higher performance skills. The data were analyzed using SPSS/WIN 22.0 program set at significance level of p<05. The effect of simulation education was much more significant than the conventional education in BLS. The simulation education is very important and effective in improving the clinical performance skills of paramedics than the conventional education. The simulation education can provide the virtual environment of cardiac arrest to the paramedics. In conclusion, the simulation education can provide the effective teaching methods for various practice performance skills and solution by critical thinking in the paramedics and healthcare providers in the future.

• 한국융합학회논문지
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• 제12권2호
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• pp.295-300
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• 2021

본 연구는 Celeriac Extract의 전 염증성 사이토카인의 생성 억제에 미치는 영향을 살펴보고 억제 정도를 확인하기 위하여 실시되었다. 염증은 전 염증성 사이토카인인 TNF-α나 IL-6, IL-1β 및 활성산소와 같은 매개인자에 의해 나타난다. 이에 RAW264.7 세포에 lipopolysaccharide(LPS) 로 자극하여 생성된 TNF-α나 IL-6, IL-1β과 같은 전염증성 사이토카인과 NO 같은 활성산소에 대한 Celeriac Extract(1ug/mL, 10ug/mL, 100ug/mL)의 영향을 살펴보았다. 그 결과 Celeriac Extract은 세포 독성 없이 TNF-α나 IL-6의 생성과 NO생성을 유의성 있게 저해하였다. 따라서 이러한 결과는 셀러리악 추출물이 전 염증성 사이토카인 및 NO 생성을 억제하여 염증 반응을 약화시킬 수 있음을 시사한다.

• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.197-206
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• 2021

microRNA-361-3p (miR-361-3p) is involved in the carcinogenesis of oral cancer and pancreatic catheter adenocarcinoma, and has anti-carcinogenic effects on non-small cell lung cancer (NSCLC). However, its effect on multiple myeloma (MM) is less reported. Here, we found that upregulating the expression of miR-361-3p inhibited MM cell viability and promoted MM apoptosis. We measured expressions of tumor necrosis factor receptor-associated factor 6 (TRAF6) and miR-361-3p in MM cells and detected the viability, colony formation rate, and apoptosis of MM cells. In addition, we measured expressions of apoptosis-related genes Bcl-2, Bax, and Cleaved caspase-3 (C caspase-3). The binding site between miR-361-3p and TRAF6 was predicted by TargetScan. Our results showed that miR-361-3p was low expressed in the plasma of MM patients and cell lines, while its overexpression inhibited viability and colony formation of MM cells and increased the cell apoptosis. Furthermore, TRAF6, which was predicted to be a target gene of miR-361-3p, was high-expressed in the plasma of patients and cell lines with MM. Rescue experiments demonstrated that the effect of TRAF6 on MM cells was opposite to that of miR-361-3p. Upregulation of miR-361-3p induced apoptosis and inhibited the proliferation of MM cells through targeting TRAF6, suggesting that miR-361-3p might be a potential target for MM therapy.

• 대한조선학회논문집
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• 제37권2호
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• pp.46-56
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• 2000

본 논문은 손상선박의 안전대책에 관한 연구의 일환으로, 황천항행중인 선박이 충돌, 좌초 등 원인에 의해 손상을 받았을 때를 가상하고, 손상선박을 다른 안전한 장소로 예항하고자 할 때의 침로안정성 문제를 다루고 있다. 외력으로는 바람의 영향만을 고려할 때, 예선 피예선계의 침로안정성 평가를 위한 특성방정식을 도출하고, 피예선의 각 손상상태에 따른 침로안정성을 수치계산하였다. 그 결과 손상상태, 풍속, 풍향 및 예항삭의 길이 등이 침로안정성에 미치는 영향을 평가할 수 있었다.

• 대한물리의학회지
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• 제10권1호
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• pp.107-113
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• 2015

PURPOSE: This study was to investigate the effect of stabilization exercise with abdominal breath on Balance and Oswestry Disability Index for Low Back Pain Patients. METHODS: The subjects were 18 low back pain patients in their twenties. The subjects were randomly assigned into experimental group and control group. Control group and experimental group both participated in 30 minutes of stabilization exercise for 8 weeks(5 times/week), and experimental group participated in stabilization exercise with abdominal breath. The subjects were tested balance and pain using BioRescue and Oswestry Diability Index respectively, before and after intervention. RESULTS: The experimental group showed a statistical significance in movement areas of the body's center(p<.05), but there was no significant differences between groups (p>.05). The experimental group showed a statistical significance in movement distances of the body's center with eyes open(p<.05), and there was significant differences between groups(p<.05). Both groups showed a statistical significance in ODI between pre and post test(p<.05) but there was no significant differences between groups(p>.05). CONCLUSION: As a result, the group participating in stabilization exercise with abdominal breath had a more effective improvement than the control group. Therefore, the stabilization exercise with abdominal breath may be used improving balance and pain in low back pain patients.